摘要：
A simple and sensitive colorimetric sensing method was constructed for detection of Hg(2+) in aqueous solutions and based on silver nanoparticles functionalized with l-cysteine (l-Cys-Ag NPs). In this method, adenosine triphosphate (ATP) induced aggregation of l-Cys-Ag NPs. Simultaneously, the solution colour changed from bright yellow to brown. In the presence of Hg(2+) , Hg(2+) chelated ATP to form a complex and reduce the degree of aggregation of l-Cys-Ag NPs and was accompanied by a colour change from brown to bright yellow. The changing values of absorbance at 390 nm were linearly correlated with concentration of Hg(2+) over the 4.00 × 10(-8) to 1.04 × 10(-6) mol·L(-1) range, with a detection limit of 8 nM. This method was used successfully for detection of Hg(2+) in real water samples and performed good selectivity and sensitivity. The recovery range was 91.5-109.1%, indicating that the method has vast application potential for determination of Hg(2+) in the environment.
摘要：
Understanding miRNAs regulatory roles in epithelial-mesenchymal transition (EMT) would help establish new avenues for further uncovering the mechanisms underlying radiation-induced pulmonary fibrosis (RIPF) and identifying preventative and therapeutic targets. Here, we demonstrated that miR-541-5p repression by Myeloid Zinc Finger 1 (MZF1) promotes radiation-induced EMT and RIPF. Irradiation could decrease miR-541-5p expression in vitro and in vivo and inversely correlated to RIPF development. Ectopic miR-541-5p expression suppressed radiation-induced-EMT in vitro and in vivo. Knockdown of Slug, the functional target of miR-541-5p, inhibited EMT induction by irradiation. The upregulation of transcription factor MZF1 upon irradiation inhibited the expression of endogenous miR-541-5p and its primary precursor (pri-miR-541-5p), which regulated the effect of the Slug on the EMT process. Our finding showed that ectopic miR-541-5p expression mitigated RIPF in mice by targeting Slug. Thus, irradiation activates MZF1 to downregulate miR-541-5p in alveolar epithelial cells, promoting EMT and contributing to RIPF by targeting Slug. Our observation provides further understanding of the development of RIPF and determines potential preventative and therapeutic targets.
摘要：
Human bronchial epithelial (HBE) cells and c-fos-silenced HBE cells were first exposed to fine particulate matter (PM2.5) and the resulting miRNA sequenced. Thereafter, a weighted gene co-expression network analysis was performed using Cytoscape software to visualize the interactions between identified hub miRNAs and their target genes. Nine differentially expressed miRNAs in hub miRNAs were identified in the different treatment groups, of which miR-25-3p, miR-215-5p, and miR-145-5p were selected for further study. Following qPCR validation, both miR-25-3p and miR-215-5p were found to be significantly up-regulated whilst, miR-145-5p was significantly down-regulated (p < 0.05) in the PM2.5 group. Furthermore, miR-25-3p and miR-145-5p were also significantly down-regulated in the untreated group of c-fos silenced HBE cells. However, miR-215-5p was significantly down-regulated in both the untreated and PM2.5-treated groups of c-fos silenced HBE cells. Subsequent analysis of their target genes also illustrated differential gene expression when comparing the treatment groups of the two cell types. The present data indicated that the c-fos gene has an important effect on the miRNA expression profiles and the related signaling pathways in PM2.5-treated HBE cells. Therefore, each of miR-25-3p, miR-145-5p, and miR-215-5p may potentially provide future research information for additional exploration of a PM2.5-induced carcinogenesis mechanism.
摘要：
A novel adsorbent magnetic functionalized m-carboxyphenyl azo calix[4]arene symmetrical sulfide derivative (Fe3O4/M-CCSSD) was fabricated by a series of simple reactions and characterized by FT-IR and SEM. The effects on U (VI) adsorption process were investigated at several conditions. The calculated data disclose that the adsorption process of U (VI) by Fe3O4/M-CCSSD is an endothermic spontaneous process, which well fits with the pseudo-second-order kinetic and Freundlich isotherm model. In the most favorable conditions, accounting for approximately 89% of U (VI) was removed. In addition, Fe3O4/M-CCSSD showed high selectivity for U (VI) and exceptional reusability.
期刊：
Molecular and Cellular Biochemistry,2021年476(12):4265-4275 ISSN：0300-8177
通讯作者：
Chen, Shenghua;Chen, Lili
作者机构：
[Bai, Qinqin; Zhou, You; Chen, Lili; Huang, Qiaoling; Chen, Li; Tong, Ting] Univ South China, Hengyang Med Sch, Coll Publ Hlth, Dept Publ Hlth,Lab Sci, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.;[Bai, Qinqin; Zhou, You; Chen, Lili; Huang, Qiaoling; Chen, Li; Tong, Ting] Univ South China, Coll Publ Hlth, Hunan Prov Key Lab Typical Environm Pollut & Hlth, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.;[Li, Zhongyu; Lu, Chunxue; Chen, Shenghua] Univ South China, Hengyang Med Sch, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Chen, Shenghua] H;[Chen, Lili] D;Hengyang Medical School, University of South China, 28 West Changsheng Rd, Hengyang, 421001, Hunan, China.;Department of Public Health Laboratory Sciences, College of Public Health, Hengyang Medical School, University of South China, 28 West Changsheng Rd, Hengyang, 421001, Hunan, China.;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, College of Public Health, University of South China, 28 West Changsheng Rd, Hengyang, 421001, Hunan, China.
关键词：
MicroRNA;Lung;Apoptosis
摘要：
MicroRNAs (miRNAs) are a type of endogenous non-coding short-chain RNA, which plays a crucial role in the regulation of many essential cellular functions, including cellular migration, proliferation, invasion, autophagy, oxidative stress, apoptosis, and differentiation. The lung can be damaged by pathogenic microorganisms, as well as physical or chemical factors. Research has confirmed that miRNAs and lung cell apoptosis can affect the development and progression of several lung diseases. This article reviews the role of miRNAs in the development of lung disease through regulating host cell apoptosis.
摘要：
Proteomics and bioinformatics were applied to explore PM(2.5)-induced differentially expressed proteins (DEPs) in hepatocytes (L02 cells) and c-Myc-silenced hepatocytes. L02 cells and c-Myc-silenced hepatocytes were treated with PM(2.5) for 24h. Fifty-two DEPs were screened in L02 hepatocytes, of which 28 were upregulated and 24 were downregulated. Forty-one DEPs were screened in the c-Myc-silenced hepatocytes, of which 31 were upregulated and 10 were downregulated. GO analysis showed that DEPs in L02 cells were mainly concentrated in the cytosol and were involved in biological processes such as the response to metal ions. DEPs in c-Myc-silenced cells were mainly enriched in the extracellular space and were involved in lipoprotein metabolism. KEGG analysis showed that DEPs in L02 cells were mainly involved in arachidonic acid metabolism and mineral absorption. DEPs in c-Myc-silenced cells were mainly enriched in pathways involving nerve absorption, complement and coagulation cascades, and other pathways. Twenty key proteins, including Metallothionein-2A (MT2A), Metallothionein-1X (MT1X), zinc transporter ZIP10 (SLC39A10) and Serine protease 23 (PRSS23) were screened in two groups through analysis of protein-protein interactions. Based on the identification of the selected DEPs, PRSS23 and SLC39A10 might be the potential biomarker of PM(2.5)-induced carcinogenesis, which provide the scientific basis for further research into the carcinogenic mechanisms of PM(2.5).
摘要：
Despite of growing evidence linking PM(2.5) exposure to autophagic activity in various human cells, the functional significance of PM(2.5) exposure affecting autophagy in the pathogenesis of human cardiovascular disease and the underlying molecular mechanisms remain unclear. In this study, the effects of ambient PM(2.5) (with final concentration 0, 1, 5, 25µg/mL) on the autophagic activity in human umbilical vein endothelial cells (HUVECs) were systematically studied. The results showed that the internalized PM(2.5) mainly localized in the membrane-surrounded vacuoles in the cytoplasm. Compared with the negative control, dose-dependent increase of autophagosomes, puncta and protein levels of LC3-II and p62, and both dose- and time-dependent increase of AKT phosphorylation, with inversely time-dependent reduction of Beclin 1, ATG3 and ATG5 proteins, were presented in the PM(2.5)-treated HUVECs, indicating a clear impairment of autophagic degradation in the PM(2.5)-exposed HUVECs. Meanwhile, increase in lysosomes, LAMP1, proteases of CTSB and CTSD, and protein phosphorylation of ERK1/2 and TFEB was identified in the PM(2.5)-treated HUVECs, showing a PM(2.5)-mediated enhancement in lysosomal activity. A novel finding in this study is that both Sntaxin-17 and LAMP2, two key proteins involved in the control of membrane fusion between autophagosome and lysosome, were significantly decreased in the PM(2.5)-exposed HUVECs, suggesting that the fusion of autophagosome-lysosome was blocked up. Collectively, ambient PM(2.5) exposure may block up the autophagic flux in HUVECs through inhibiting the expression of Sntaxin-17 and LAMP2. Autophagic activity in HUVECs is a useful biomarker for assessing risks of environmental factors to human cardiovascular health.
期刊：
Journal of Radioanalytical and Nuclear Chemistry,2021年328(3):1265-1278 ISSN：0236-5731
通讯作者：
Fangzhu Xiao<&wdkj&>Guowen Peng
作者机构：
[Luo, Jiaqi; Peng, Guowen] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Fangzhu; Zhu, Qiqi; Li, Shanshan] Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Chen, Fang; Peng, Guowen] Univ South China, Hunan Prov Engn Technol Res Ctr Uranium Tailings, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Fangzhu; Peng, Guowen] Univ South China, Hunan Engn Res Ctr Safety Control & Recycling Rad, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Fangzhu Xiao; Guowen Peng] S;School of Public Health, University of South China, Hengyang, People’s Republic of China<&wdkj&>Hunan Engineering Research Center for the Safety Control and Recycling of Radioactive Heavy Metal Pollutants, University of South China, Hengyang, People’s Republic of China<&wdkj&>School of Chemistry and Chemical Engineering, University of South China, Hengyang, People’s Republic of China<&wdkj&>Hunan Province Engineering Technology Research Center of Uranium Tailings Treatment, University of South China, Hengyang, People’s Republic of China<&wdkj&>Hunan Engineering Research Center for the Safety Control and Recycling of Radioactive Heavy Metal Pollutants, University of South China, Hengyang, People’s Republic of China
摘要：
In order to solve the problem of lack of resistance of active microorganisms to the complex ion environment in the treatment of uranium-containing wastewater, the Deino-flr-2 radiation-resistant genetically engineered bacteria containing flr-2 fluorine-resistant gene was constructed and characterized. Utilizing the reductase activity of Deinococcus radiodurans (DR) itself and the enrichment of U(VI) by the surface active functional groups of the bacteria, the treatment of simulated low-concentration uranium-containing wastewater will be realized. In the most favorable conditions, accounting for approximately 90% of U(VI) was removed. The calculated data disclosed that the enrichment process of U(VI) by Deino-flr-2 well fit with the pseudo-second-order kinetic and Freundlich isotherm model.
作者机构：
[Xu, Yue-Xin; Wan, Yi-Le; Hao, Yu-E] Univ South China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Bao, Dan-Feng; Su, Hong-Yan; Wan, Yi-Le; Luo, Zong-Long] Dali Univ, Coll Agr & Biol Sci, Dali 671003, Yunnan, Peoples R China.;[Bao, Dan-Feng] Mae Fah Luang Univ, Ctr Excellence Fungal Res, Chiang Rai 57100, Thailand.;[Bao, Dan-Feng] Chiang Mai Univ, Fac Agr, Dept Entomol & Plant Pathol, Chiang Mai 50200, Thailand.;[Bhat, Darbhe-Jayarama] Azad Coop Housing Soc, 128-1-J, Curca 403108, Goa Velha, India.
通讯机构：
[Hao, Yu-E] U;[Su, Hong-Yan] D;Univ South China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;Dali Univ, Coll Agr & Biol Sci, Dali 671003, Yunnan, Peoples R China.
关键词：
2 new taxa;freshwater fungi;morphology;phylogeny;taxonomy
摘要：
During a survey on diversity of freshwater fungi along a north-south latitudinal gradient in Asia, three fresh specimens of Minimelanolocus were collected from submerged wood in streams in northwestern Yunnan Province, China. Based on their distinct morphological features and phylogenetic analyses of combined ITS, LSU and SSU sequence data, Minimelanolocus nujiangensis and M. clavatus are described as new species and M. submersus was recollected from Yunnan, China. Illustrations and descriptions with notes of the three species are provided. This study increases the known diversity of Minimelanolocus and enriches freshwater fungal resources.
摘要：
Purpose: To explore the esophageal cancer (EC) incidence and mortality trends and risk factors in China during 2005-2015. Materials and Methods: The data were stratified by area (urban, rural), gender (male, female), and age groups (0 similar to, 5 similar to, ..., 85 similar to). The age-standardized incidence rate (ASIR) and mortality rate (ASMR), age-specific incidence and mortality were calculated to describe the trends, which were analyzed by Joinpoint software, negative binomial regression model, and age-period-cohort model. Results: Trends in EC ASIR decreased markedly during 2010-2015 (APC=-6.14%, P<0.05), and the average annual percent change (AAPC) value was -8.07% (95% confidence interval (CI): -9.98 similar to-6.12) for rural areas during 2005-2015. The ASMR was on a fast-downward trend after 2011 (APC=-6.67%, P<0.05), with AAPC values of -1.34% (95% CI: - 2.56 similar to-0.19) for males, -3.39% (95% CI: -5.65, -1.07) for females, and -9.67% (95% CI: - 10.56 similar to-8.77) for rural areas during 2005-2015. The age-specific incidence and mortality increased with age. The risk of EC for males was 3.1675 times higher than females (P<0.001), and for urban areas, it was 0.58 times larger than rural (P<0.001). The age and period effects presented an increasing trend, with a decreasing trend for the cohort effects in incidence and mortality risk. Later birth cohorts presented lower risks than previous birth cohorts. Conclusion: ASIR and ASMR in China are higher in males than females, and higher in rural than urban areas, which have decreased during 2005-2015, especially in rural areas. The incidence increased with age up to the peak age group of 75. Area, gender, and age were independent risk factors for EC incidence.
摘要：
Deinococcus radiodurans (DR) was used as a biological adsorbent for uranium adsorption of low-concentration uranium wastewater.The enrichment performance of the DR was investigated at several conditions,and characterized by SEM and EDS, genes with relatively different expression of DR enriched uranium were screened through RNA-seq. Through GO analysis and KEGG pathway enrichment analysis, to find the main regulatory pathways.The results show that: The DR under the culture condition, pH = 5, temperature 30 celcius, initial concentration of 10 mg/L, dosage of DR 16 mL, adsorption time for 60 min, uranium(VI) adsorption rate was 91%, the solution pH = 6.1 after enrichment; several genes were up-regulated or down-regulated, which involved in a variety of signaling pathways, mainly involving oxidative phosphorylation, various glucose metabolism and amino acid synthesis metabolism. Cytochrome C oxidase was significantly up-regulated.
摘要：
Chlamydia psittaci is an important zoonotic factor associated with human and animal atypical pneumonia. Resisting host cell apoptosis is central to sustaining Chlamydia infection in vivo. Chlamydia can secrete inclusion membrane proteins (Incs) that play important roles in their development cycle and pathogenesis. CPSIT_0846 is an Inc protein in C. psittaci identified by our team in previous work. In the current study, we investigated the regulatory role of CPSIT_0846 in HeLa cell apoptosis, and explored potential mechanisms. The results showed that HeLa cells treated with CPSIT_0846 contained fewer apoptotic bodies and exhibited a lower apoptotic rate than untreated cells either with Hoechst 33258 fluorescence staining or flow cytometry with or without induction by staurosporine (STS). CPSIT_0846 could increase the phosphorylation of the extracellular signal-regulated kinases 1/2 (ERK1/2) or stress-activated protein kinases/c-Jun amino-terminal kinases (SAPK/JNK) signaling pathways, and the Bcl-2 associated X protein (Bax)/B cell lymphoma 2 (Bcl-2) ratio, levels of cleaved caspase-3/9 and cleaved Poly-ADP-ribose polymerase (PARP) were significantly up-regulated following inhibition of ERK1/2 or SAPK/JNK pathways with U0126 or SP600125. After carbonyl cyanide 3-chlorophenylhydrazone (CCCP) treatment, the mitochondrial membrane potential (MMP) of cells was significantly decreased in control group, but stable in the CPSIT_0846 treated one, and less cytochrome c (Cyt.c) was released into the cytoplasm. Inhibition of the ERK1/2 or SAPK/JNK pathway significantly decreased the JC-1 red-green fluorescence signal, and promoted Cyt.c discharge into the cytoplasm in HeLa cells treated with CPSIT_0846. In conclusion, CPSIT_0846 can regulate mitochondrial pathway-mediated apoptosis in HeLa cells by activating the ERK/JNK signaling pathway.
摘要：
The success of graphene oxide (GO) has attracted extensive research interests in developing novel 2D nanomaterials (NMs). Graphdiyne (GDY) is a new member of carbon-based 2D NMs possessing sp- and sp(2) -hybridized carbon atoms. However, the toxicity of GDY is less investigated as GO. In this study, we compared the toxicity of GDY and GO with human umbilical vein endothelial cells (HUVECs). Exposure to up to 100-μg/ml GDY and GO induced cytotoxicity, but there was no statistically significant difference between GDY and GO. At noncytotoxic concentration, 25-μg/ml GDY or GO led to the internalization of NMs, typically in cytoplasm but not in nuclei. Only GO but not GDY significantly increased THP-1 adhesion onto NM-exposed HUVECs. Meanwhile, compared with GDY, GO more effectively promoted the release of soluble intracellular cell adhesion molecule-1 (sICAM-1), indicating the differential effects of GDY and GO on endothelial activation. Neither GDY nor GO induced intracellular superoxide. However, GO significantly promoted the expression of endoplasmic reticulum (ER) stress genes activating transcription factor 4 (ATF4) and X-box binding protein 1 spliced (XBP-1s), as well pyroptosis genes NLR family pyrin domain containing 3 (NLRP3) and gasdermin D (GSDMD), whereas GDY did not show this effect. The results suggested that GDY and GO could be internalized into HUVECs leading to cytotoxic effects. However, GO was more potent to activate endothelial activation probably due to the activation of ER stress and pyroptosis genes.
通讯机构：
[Long, Ding-Xin] U;Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
摘要：
The biological effects and regulatory mechanisms of low-dose and low-dose-rate radiation are still rather controversial. Therefore, in this study we investigated the effects of low-dose-rate radiation on zebrafish neurodevelopment and the role of miRNAs in radiation-induced neurodevelopment. Zebrafish embryos received prolonged gamma-ray irradiation (0 mGy/h, 0.1 mGy/h, 0.2 mGy/h, 0.4 mGy/h) during development. Neurodevelopmental indicators included mortality, malformation rate, swimming speed, as well as the morphology changes of the lateral line system and brain tissue. Additionally, spatiotemporal expression of development-related miRNAs (dre-miR-196a-5p, dre-miR-210-3p, dre-miR-338) and miRNA processing enzymes genes (Dicer and Drosha) were assessed by qRT-PCR and whole mount in situ hybridization (WISH). The results revealed a decline in mortality, malformation and swimming speed, with normal histological and morphological appearance, in zebrafish that received 0.1 mGy/h; however, increased mortality, malformation and swimming speed were observed, with pathological changes, in zebrafish that received 0.2 mGy/h and 0.4 mGy/h. The expression of miRNA processing enzyme genes was altered after irradiation, and miRNAs expression was downregulated in the 0.1 mGy/h group, and upregulated in the 0.2 mGy/h and 0.4 mGy/h groups. Furthermore, ectopic expression of dre-miR-210-3p, Dicer and Drosha was also observed in the 0.4 mGy/h group. In conclusion, the effect of low-dose and low-dose-rate radiation on neurodevelopment follows the threshold model, under the regulation of miRNAs, excitatory effects occurred at a dose rate of 0.1 mGy/h and toxic effects occurred at a dose rate of 0.2 mGy/h and 0.4 mGy/h. (C) 2021 by Radiation Research Society
作者：
Wei, Jia;Huang, Feiyu;Feng, Hai;Massey, Isaac Yaw;Clara, Tezi;...
期刊：
Frontiers in Microbiology,2021年12:646084 ISSN：1664-302X
通讯作者：
Yang, F.
作者机构：
[Wei, Jia; Massey, Isaac Yaw; Huang, Feiyu; Feng, Hai; Clara, Tezi; Yang, Fei; Luo, Jiayou] Cent South Univ, Xiangya Sch Publ Hlth, Hunan Prov Key Lab Clin Epidemiol, Changsha, Peoples R China.;[Long, Dingxin; Yang, Fei; Cao, Yi] Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang, Peoples R China.;[Yang, Fei] Southeast Univ, Key Lab Environm Med Engn, Minist Educ, Sch Publ Hlth, Nanjing, Peoples R China.
通讯机构：
[Yang, F.] H;[Yang, F.] K;Hunan Provincial Key Laboratory of Clinical Epidemiology, China;Key Laboratory of Environmental Medicine Engineering, China;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, China
摘要：
Microcystins (MCs) are extremely hazardous to the ecological environment and public health. How to control and remove MCs is an unsolved problem all over the world. Some microbes and their enzymes are thought to be effective in degrading MCs. Microcystinase can linearize microcystin-leucine-arginine (MC-LR) via a specific locus. However, linearized MC-LR is also very toxic and needs to be removed. How linearized MC-LR was metabolized by linearized-microcystinase, especially how linearized-microcystinase binds to linearized MC-LR, has not been defined. A combination of in vitro experiments and computer simulation was applied to explore the characterization and molecular mechanisms for linearized MC-LR degraded by linearized-microcystinase. The purified linearized-microcystinase was obtained by recombinant Escherichia coli overexpressing. The concentration of linearized MC-LR was detected by high-performance liquid chromatography, and linearized MC-LR degradation products were analyzed by the mass spectrometer. Homology modeling was used to predict the structure of the linearized-microcystinase. Molecular docking techniques on the computer were used to simulate the binding sites of linearized-microcystinase and linearized MC-LR. The purified linearized-microcystinase was obtained successfully. The linearized-microcystinase degraded linearized MC-LR to tetrapeptide efficiently. The second structure of linearized-microcystinase consisted of many alpha-helices, beta-strands, and colis. Linearized-microcystinase interacted the linearized MC-LR with hydrogen bond, hydrophobic interaction, electrostatic forces, and the Van der Waals force. This study firstly reveals the characterization and specific enzymatic mechanism of linearized-microcystinase for catalyzing linearized MC-LR. These findings encourage the application of MC-degrading engineering bacteria and build a great technique for MC-LR biodegradation in environmental engineering.
