作者机构：
[Xu, Jia; Zhang, Chang-E] Guangzhou Med Univ, Affiliated Canc Hosp, Guangzhou Municipal & Guangdong Prov Key Lab Prot, State Key Lab Resp Dis,Sch Basic Med Sci, Guangzhou 510000, Guangdong, Peoples R China.;[He, Kaiwu; Nie, Lulin; Liu, Jianjun; Xu, Jia; Dan, Ding; Yang, Xifei; Zhang, Kaiqin] Shenzhen Ctr Dis Control & Prevent, Key Lab Modern Toxicol Shenzhen, Shenzhen Med Key Subject Modern Toxicol, Shenzhen 518055, Peoples R China.;[Zhang, Kaiqin] Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Yang, Chao] Shenzhen Univ, Cognit Impairment Ward Neurol Dept, Affiliated Hosp 3, Med Coll, Shenzhen 518001, Peoples R China.
通讯机构：
[Jia Xu; Chang-E. Zhang] G;[Ding Dan; Lulin Nie; Kaiwu He; Jianjun Liu; Kaiqin Zhang; Xifei Yang] K;[Chao Yang] C;Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation,State Key Laboratory of Respiratory Disease,School of Basic Medical Sciences,China<&wdkj&>Key Laboratory of Modern Toxicology of Shenzhen,Shenzhen Medical Key Subject of Modern Toxicology,Shenzhen Center for Disease Control and Prevention,China<&wdkj&>Cognitive Impairment Ward of Neurology Department,The Third Affiliated Hospital of Shenzhen University Medical College,Shenzhen 518001,China<&wdkj&>Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation,State Key Laboratory of Respiratory Disease,School of Basic Medical Sciences,China<&wdkj&>Key Laboratory of Modern Toxicology of Shenzhen,Shenzhen Medical Key Subject of Modern Toxicology,Shenzhen Center for Disease Control and Prevention,China<&wdkj&>Key Laboratory of Modern Toxicology of Shenzhen,Shenzhen Medical Key Subject of Modern Toxicology,Shenzhen Center for Disease Control and Prevention,China<&wdkj&>School of Public Health,University of South China,Hengyang Hunan 421001,China
关键词：
Introduction;Materials and Methods;Results;Discussion;Conclusion;Abstract;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interests;Authors’ Contributions;Funding Statement;Acknowledgements;Acknowledgments;Supplementary Materials;Reference;Dataset Description;Dataset Files;Abstract;Introduction;Introduction and Materials;Introduction and Methods;Materials;Materials and Methods;Methods;Results;Discussion;Results and Discussion;Discussion and Conclusion;Results and Conclusion;Conclusion;Conclusions;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interest;Authors’ Contributions;Funding Statement;Acknowledgements;Supplementary Materials;References;Appendix;Abbreviations;Preliminaries;Introduction and Preliminaries;Notation;Proof of Theorem;Proofs;Analysis of Results;Examples;Numerical Example;Applications;Numerical Simulation;Model;Model Formulation;Systematic Palaeontology;Nomenclatural Acts;Taxonomic Implications;Experimental;Synthesis;Overview;Characterization;Background;Experimental;Theories;Calculations;Model Verification;Model Implementation;Geographic location;Study Area;Geological setting;Data Collection;Field Testing;Data and Sampling;Dataset;Literature Review;Related Works;Related Work;System Model;Methods and Data;Experimental Results;Results and Analysis;Evaluation;Implementation;Case Presentation;Case Report;Search Terms;Case Description;Case Series;Background;Limitations;Additional Points;Case;Case 1;Case 2 etc.;Concern Details;Retraction Details;Copyright;Related Articles
摘要：
Depression is one of the most common neuropsychiatric disorders. Although the pathogenesis of depression is still unknown, environmental risk factors and genetics are implicated. Copper (Cu), a cofactor of multiple enzymes, is involved in regulating depression-related processes. Depressed patients carrying the apolipoprotein ε4 allele display more severe depressive symptoms, indicating that ApoE4 is closely associated with an increased risk of depression. The study explored the effect of low-dose Cu exposure and ApoE4 on depression-like behavior of mice and further investigates the possible mechanisms. The ApoE4 mice and wild-type (WT) mice were treated with 0.13 ppm CuCl2 for 4 months. After the treatment, ApoE4 mice displayed obvious depression-like behavior compared with the WT mice, and Cu exposure further exacerbated the depression-like behavior of ApoE4 mice. There was no significant difference in anxiety behavior and memory behavior. Proteomic analysis revealed that the differentially expressed proteins between Cu-exposed and nonexposed ApoE4 mice were mainly involved in the Ras signaling pathway, protein export, axon guidance, serotonergic synapse, GABAergic synapse, and dopaminergic synapse. Among these differentially expressed proteins, immune response and synaptic function are highly correlated. Representative protein expression changes are quantified by western blot, showing consistent results as determined by proteomic analysis. Hippocampal astrocytes and microglia were increased in Cu-exposed ApoE4 mice, suggesting that neuroglial cells played an important role in the pathogenesis of depression. Taken together, our study demonstrated that Cu exposure exacerbates depression-like behavior of ApoE4 mice and the mechanisms may involve the dysregulation of synaptic function and immune response and overactivation of neuroinflammation.
摘要：
Using renewable microalgal biomass as active feedstocks for biofuels and bioproducts is explored to substitute petroleum-based fuels and chemicals. In the last few years, the importance of microalgae biomass has been realized as a renewable feedstock due to several positive attributes associated with it. Biorefinery via anaerobic digestion (AD) of microalgal biomass is a promising and sustainable method to produce value-added chemicals, edible products and biofuels. Microalgal biomass pretreatment is a significant process to enhance methane production by AD. Findings on the AD microbial community's variety and organization can give novel in turn on digester steadiness and presentation. This review presents a vital study of the existing facts on the AD microbial community and AD production. Co-digestion of microalgal biomass with different co-substrates was used in AD to enhance biogas production, and the process was economically viable with improved biodegradability. Microcystins, which are produced by toxic cyanobacterial blooms, create a severe hazard to environmental health. Anaerobic biodegradation is an effective method to degrade the microcystins and convert into nontoxic products. However, for the cost-effective conversion of biomass to energy and other beneficial byproducts, additional highly developed research is still required for large-scale AD of microalgal biomass.
期刊：
Current Microbiology,2021年78(9):3577-3585 ISSN：0343-8651
通讯作者：
Jang-Seu Ki
作者机构：
[Wang, Hui; Ki, Jang-Seu] Sangmyung Univ, Dept Biotechnol, Seoul 03016, South Korea.;[Wang, Hui] Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang 421001, Peoples R China.
通讯机构：
[Jang-Seu Ki] D;Department of Biotechnology, Sangmyung University, Seoul, South Korea
摘要：
Programmed cell death (PCD) in dinoflagellates has been introduced as a new concept that facilitates the demise of harmful algal blooms. Metacaspases (MCAs) play a role in PCD, but their function in dinoflagellates is unclear. Here, we cloned a novel MCA gene (PmMCA) from the harmful dinoflagellate Prorocentrum minimum and examined its molecular characteristics and gene expression during cell death. The gene was encoded in the nuclear genome with two introns. The putative protein contained 288 amino acids and three conserved MCA signature motifs. Phylogenetic analysis showed that PmMCA may have the same ancestor as other dinoflagellates. PmMCA expression and cell apoptosis were significantly induced under copper exposure, considerably affecting cell growth. These results suggest that PmMCA could be involved in PCD triggered by copper stress.
作者机构：
[Feng, Shui-Dong; Yang, Zheng-Rong; Mao, Sha-Sha; Tang, Jie] Univ South China, Sch Publ Hlth, Hengyang, Hunan, Peoples R China.;[Zhao, Jin; Zheng, Chen-Li; Li, Hao; Yang, Zheng-Rong; Mao, Sha-Sha; Tang, Jie] Shenzhen Ctr Dis Control & Prevent, Dept HIV AIDS Control & Prevent, Shenzhen, Guangdong, Peoples R China.;[Hu, Wei] Shenzhen Ctr Chron Dis Control, Shenzhen, Guangdong, Peoples R China.
摘要：
OBJECTIVES: To analyze the trends of HIV/syphilis/HSV-2 seropositive rate and explore the related factors with HSV-2 infection to provide the basis for adjusting STD intervention strategies and formulating prevention and control measures among MSM in Shenzhen. METHODS: Time-location sampling was conducted among MSM in Shenzhen in 2012, 2014, 2016, and 2018. Data on demographics, sexual behaviors and the laboratory test results of HIV, syphilis, HSV-2 were collected. The χ2 trend test was used to analyze the trends of HIV/syphilis/HSV-2 seropositive rate. The binary logistic regression model was used to explore the factors associated with HSV-2 infection. RESULTS: The seropositive rate of HIV fell significantly from 15.9% in 2012 to 8.7% in 2018 (Ptrend = 0.003), syphilis seropositive rate was significantly decreased from 20.4% in 2012 to 14.8% in 2018 (Ptrend = 0.025), HSV-2 seropositive rate had no significant change (16.7% in 2012 to 14.0% in 2018; Ptrend = 0.617). In principal component logistic regression analysis showed that FAC1_1 (X1 = Ever had sex with female, X2 = Gender of first sexual partner, X3 = Marital status, X4 = Age group), FAC2_1 (X5 = Education, X6 = Monthly income (RMB), X7 = Frequency of condom use in anal sex with men in the past 6 months), and FAC4_1 (X9 = History of STDs) were significantly associated with HSV-2 infection. CONCLUSIONS: The seropositive rates of HIV and syphilis have dropped significantly but are still high. HSV-2 seropositive rate had no significant change and maintained a high level. It is necessary to continue strengthening HIV and syphilis interventions among MSM in Shenzhen. HSV-2 detection and intervention are urgently required for MSM, which might be another effective biological strategy further to control the HIV epidemic among MSM in Shenzhen.
摘要：
Burning municipal waste produces a great deal of harmful heavy metals, which may lead to elevated exposure in incinerator workers and residents living nearby. However, relevant human exposure studies remain scarce, especially in China. This study aimed to determine the concentrations of ten heavy metals in urine of incinerator workers (n = 119, as the exposed group) and residents living nearby (n = 215, as the control group) from Shenzhen (China), and explore the associations between heavy metal exposure and DNA oxidative stress (indicated by 8-hydroxy-2'-deoxyguanosine or 8-OHdG) in humans. The median urinary concentrations of manganese (Mn), iron (Fe), chromium (Cr), arsenic (As), selenium (Se) and 8-OHdG in the exposed group were significantly higher than those in the control group (p < 0.05), suggesting occupational exposure elevated heavy metal intake for the incinerator workers. Nevertheless, there is no correlation statistically significant between the concentrations of any heavy metal and 8-OHdG in urine in the exposed group, indicating heavy metals releasing from waste incineration were not important factors to induce DNA oxidative stress. To our knowledge, this study firstly reported the concentrations of heavy metal in urine and their associations with DNA oxidative damage in waste incinerator workers in Shenzhen, China. (C) 2020 Elsevier Ltd. All rights reserved.
摘要：
A superior electrochemical biosensor was designed for the determination of UO2(2+) in aqueous solution by integration of DNAzyme and DNA-modified gold nanoparticle (DNA-AuNP) network structure. Key features of this method include UO2(2+) inducing the cleavage of the DNAzyme and signal amplification of DNA-AuNP network structure. In this electrochemical method, the DNA-AuNP network structure can be effectively modified on the surface of gold electrode and then employed as an ideal signal amplification unit to generate amplified electrochemical response by inserting a large amount of electrochemically active indicator methylene blue (MB). In the presence of UO2(2+), the specific sites on DNA-AuNP network structure can be cleaved by UO2(2+), releasing the DNA-AuNP network structure with detectable reduction of electrochemical response intensity. The electrochemical response intensity is related to the concentration of UO2(2+). The logarithm of electrochemical response intensity and UO2(2+) concentration showed a wide linear range of 10~100 pM, and the detection limit reached 8.1 pM (S/N = 3). This method is successfully used for determination of UO2(2+) in water samples. Graphical abstract Fabricated DNAzyme network structure for enhanced electrical signal. Numerical experiments show that the current signal decreases as the concentration of UO2(2+) increases. It can be seen that the biosensors could be used to detect UO2(2+) in aqueous solution effectively.
摘要：
Cyanobacterial blooms triggered by eutrophication and climate change have become a global public health issue. The toxic metabolites microcystins (MCs) generated by cyanobacteria can accumulate in food chain and contaminate water, thus posing a potential threat to human and animals health. Studies have suggested that aside liver, the kidney may be another target organ of MCs intoxication. Therefore, this review provides various evidences on the nephrotoxicity of MCs. The review concludes that nephrotoxicity of MCs may be related to inhibition of protein phosphatases and excessive production of reactive oxygen species, cytoskeleton disruption, endoplasmic reticulum stress, DNA damage and cell apoptosis. To protect human from MCs toxic consequences, this paper also puts forward some directions for further research.
摘要：
Objective To screen the differentially expressed proteins (DEPs) in human bronchial epithelial cells (HBE) treated with atmospheric fine particulate matter (PM2.5). Methods HBE cells were treated with PM2.5 samples from Shenzhen and Taiyuan for 24 h. To detect overall protein expression, the Q Exactive mass spectrometer was used. Gene ontology (GO), Kyoto encyclopedia of genes and genomes (KEGG), and Perseus software were used to screen DEPs. Results Overall, 67 DEPs were screened in the Shenzhen sample-treated group, of which 46 were upregulated and 21 were downregulated. In total, 252 DEPs were screened in the Taiyuan sample-treated group, of which 134 were upregulated and 118 were downregulated. KEGG analysis demonstrated that DEPs were mainly enriched in ubiquitin-mediated proteolysis and HIF-1 signal pathways in Shenzhen PM2.5 samples-treated group. The GO analysis demonstrated that Shenzhen sample-induced DEPs were mainly involved in the biological process for absorption of various metal ions and cell components. The Taiyuan PM2.5-induced DEPs were mainly involved in biological processes of protein aggregation regulation and molecular function of oxidase activity. Additionally, three important DEPs, including ANXA2, DIABLO, and AIMP1, were screened. Conclusion Our findings provide a valuable basis for further evaluation of PM2.5-associated carcinogenesis.
作者机构：
[Zhang Zhao Hui; Cai Ying; Li Run Bing; Zheng Kai] Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Ji Jia Jia; Yu Shu Yuan; Li Bo Ru; Wu De Sheng; Xu Xin Yun; Qin Shuang Jian; Liu Ning; Cai Ying; Li Run Bing; Zheng Kai; Yang Chen] Shenzhen Ctr Dis Control & Prevent, Inst Environm & Hlth, Shenzhen 518055, Guangdong, Peoples R China.;[Li Bo Ru; Qin Shuang Jian] Cent South Univ, Xiangya Sch Publ Hlth, Changsha 410078, Hunan, Peoples R China.
通讯机构：
[Zhang Zhao Hui] U;[Xu Xin Yun] S;Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;Shenzhen Ctr Dis Control & Prevent, Inst Environm & Hlth, Shenzhen 518055, Guangdong, Peoples R China.
摘要：
PM_(2.5) are fine inhalable particles with diameters that are generally 2.5 micrometers and smaller and are characterized by a small particle size, a large surface area, and a strong toxin absorption ability[1-3]. PM_(2.5) contains heavy metals and organic pollutants that are harmful to human health and exert carcinogenic, teratogenic, and mutagenic effects[4-7]. PM_(2.5) has been classified as a human carcinogen by the International Agency for Research on Cancer (IARC)[2]. The economy has developed rapidly making air pollution a serious environmental health problem in China. The purpose of this study was to explore the differentially expressed genes and pathways after a PM_(2.5) exposure in the human bronchial epithelial (HBE) cells via gene chip technology and bioinformatics analysis.
通讯机构：
[Long, Ding-Xin] U;Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
autophagy;OPIDN;protein kinase C alpha;tri-ortho-cresyl phosphate;ubiquitin-proteasome system
摘要：
As an organophosphorus ester, tri-ortho-cresyl phosphate (TOCP) has been widely used in agriculture and industry. It is reported that TOCP can induce organophosphate-induced delayed neuropathy (OPIDN) in sensitive animal and human species. However, the exact molecular mechanisms underlying TOCP-induced neurotoxicity are still unknown. In this study, we found that TOCP could induce autophagy by activating protein kinase C alpha (PKC alpha) signaling in neuroblastoma SK-N-SH cells. PKC alpha activators could positively regulate TOCP-induced autophagy by increasing the expression levels of neighbor BRCA1 gene protein 1 (NBR1), LC3 and P62 autophagic receptor protein. Furthermore, PKC alpha activation impaired the ubiquitin-proteasome system (UPS), resulting in inhibition of proteasome activity and accumulation of ubiquitinated proteins. UPS dysfunction could stimulate autophagy to serve as a compensatory pathway, which contributed to the accumulation of the abnormally hyperphosphorylated tau proteins and degradation of impaired proteins of the MAP 2 and NF-H families in neurodegenerative disorders.
摘要：
A novel material called m-carboxyphenyl azo calix[4]arene amine oxime derivatives (M-CCAOD) was synthesized by diazotization-coupling reaction and substitution reaction. Then the composite consisting of Fe3O4 and M-CCAOD was prepared through a facile self-assembly method. The as-synthesized Fe3O4/M-CCAOD composite was used to remove U(VI) from aqueous solutions under different conditions. The calculated data indicate that the adsorption process of uranium by Fe3O4/M-CCAOD is an exothermic spontaneous process, which well fits with the pseudo-second-order and Freundlich model. More significantly, the composite showed high selectivity for uranium and excellent reusability, demonstrating that Fe3O4/M-CCAOD may be a potential uranium adsorbent material.
摘要：
Uranium is a kind of natural radionuclide, when the human body is exposed to higher uranium radioactive conditions, it will cause adverse effects on their health. Therefore, the analysis and detection of uranyl ions in the environment is a vital prerequisite for preventing uranium pollution. A bipolar bidentate ligand isophthalaldehyde-tetrapyrrole (IPTP) was first synthesized and a study was characterized by its structure and spectral properties. In the present work, an innovative electrochemical sensor by using IPTP as a ligand for uranyl (VI) ion (UO22+), alpha-methacrylic acid as functional monomer and uranyl (VI) ion as a template, a relatively stable structure was constructed by stirring at 35 degrees C. Then, the polymer sol-gel was acquired by combining sol-gel processing under alkaline conditions, which was added dropwise to the surface of a carbon paste electrode (CPE) which had been modified with graphene (GR). The introduction of graphene allowed the reduction peak current of uranyl ions to be increased significantly. Under the optimum experimental conditions (pH=5 and the adsorption time=20 min), the ion-imprinted sensor was designed by adopting differential pulse voltammetry (DPV) for the sensing of uranyl (VI) ions. The detection technique successfully achieved the determination of uranyl ion in the concentration range 1.0x10(-10) mol L-1 to 1.0x10(-7) mol L-1, in practical soil samples with excellent repeatability and recovery (97.3%-102.9%).
