塞来昔布诱导人椎间盘细胞表达神经生长因子
作者:
陈小明;周润梅;欧斌;曹奇
期刊:
基础医学与临床 ,2014年34(7):945-949 ISSN:1001-6325
作者机构:
[陈小明; 欧斌; 曹奇] 南华大学附属第二医院脊柱外科;[周润梅] 南华大学药学与生命科学学院药物药理研究所
关键词:
神经生长因子;椎间盘细胞;前列腺素E_2;塞来昔布
摘要:
目的研究环氧化酶2抑制剂塞来昔布以及前列腺素E_2(PGE_2)对神经生长因子(NGF)表达的影响。方法分离培养人椎间盘(IVD)细胞,用不同浓度的塞来昔布预处理30 min后,加入10 ng/mL IL-1β作用6 h。RT-PCR和ELISA分别检测NGF mRNA和蛋白的表达,ELISA检测PGE_2的分泌。同时加入外源性PGE_2以及其受体(EPs 1~4)激动剂,观察其对NGF产生的影响。结果IL-1β作用3 h即可以一定的时间依赖性诱导IVD细胞表达NGF mRNA。塞来昔布处理后能使NGF mRNA水平进一步增加1.8倍。IL-1β处理后,PGE_2含量达(5.46±0.64)ng/mL。10 mmol/L塞来昔布能将其降低至(1.07±0.04)ng/mL(P<0.05)。IVD细胞经100 μmol/L PGE_2处理后,IL-1β诱导的NGF降低了59%(P<0.05)。此外,采用EP2和EP4受体激动剂处理IVD细胞后,也得到了类似的结果,而EP1和EP3激动剂处理对NGF产生无明显影响。结论塞来昔布能促进IVD细胞表达NGF,其机制可能与抑制PGE_2的分泌有关。
语种:
中文
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某铀尾矿区植物组成及其对铀的积累作用研究
作者:
谢红艳;胡劲松;殷杰;丁德馨
期刊:
原子能科学技术 ,2014年48(11):1954-1959 ISSN:1000-6931
通讯作者:
Xie, Hong-Yan
作者机构:
[殷杰; 谢红艳; 胡劲松] School of Pharmacy and Biological Sciences, University of South China, Hengyang, China;[殷杰; 丁德馨] Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, China
通讯机构:
School of Pharmacy and Biological Sciences, University of South China, Hengyang, China
关键词:
植物组成;超富集植物;铀;植物修复
摘要:
分析研究了某铀尾矿区植物资源,并对这些植物的铀积累作用进行了研究。结果显示,该尾矿区污染土壤上植物群落较简单,共有高等植物31种,隶属12科,其中:禾本科最多,12种;其次是菊科植物,5种;凤尾蕨科和莎草科植物各3种;其他科各1种。1年生或多年生草本植物有28种,占总数的90.3%,其他3种为灌木或小乔木。对这31种植物进行了铀含量测定,结果发现,铀富集量在200 mg/kg以上的植物有14种,占总数的45%,其中富集量在600 mg/kg以上的有3种,分别是水莎草、牧草、小飞蓬。植物体内铀迁移系数大于1的有9种,其中燕麦、牧草、鼠曲草、青蒿中铀的迁移系数较大。这31种植物中,可考虑将水莎草、牧草作为超富集植物应用于铀污染土壤的修复,小飞蓬、盐肤木、枸骨、燕麦、鼠曲草、碎米莎草、龙葵等对铀污染土壤的修复具有潜在应用价值,可进一步研究。
语种:
中文
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大孔吸附树脂联合葡聚糖凝胶Sephadex LH20分离制备鹅膏肽类毒素的研究
作者:
胡劲松;陈作红
期刊:
菌物学报 ,2014年33(3):549-559 ISSN:1672-6472
作者机构:
湖南师范大学生命科学学院 湖南长沙410081;南华大学生物研究所 湖南衡阳421001;[胡劲松; 陈作红] 湖南师范大学
关键词:
大孔吸附树脂;高效液相色谱;鹅膏毒肽;鬼笔毒肽
摘要:
鹅膏菌肽类毒素是蘑菇中毒导致死亡的最主要因素,同时也是生物学研究领域的一种重要工具试剂,但由于其结构相似,分离制备单体化合物比较困难。通过4种大孔吸附树脂对致命鹅膏Amanita exitialis 子实体中主要肽类毒素组分的静态吸附与解吸附性能比较,结果表明XAD16的吸附能力最强,同时也有较强的解吸附能力,但XAD16柱层析梯度洗脱时对毒素分离效果差,表明XAD16只具有吸附富集鹅膏肽类毒素的特性但不适于分离。经XAD16柱层析富集的毒素成分,通过葡聚糖凝胶sephadex LH20柱层析分离,结果表明sephadex LH20柱层析对鹅膏肽类毒素具有较好的分离效果,经过2次sephadex LH20柱层析,获得了5个纯度达50%–80%的鹅膏肽类毒素单体化合物,进一步利用半制备高效液相色谱系统(HPLC)纯化,分离得到了7个纯度达95%以上的鹅膏肽类毒素单体,其中5个经质谱分析鉴定为:α-鹅膏毒肽(α-amanitin)、β-鹅膏毒肽(β-amanitin)、脱氧二羟毒伞素(desoxoviroidin)、羧基三羟鬼笔毒肽(phallisacin)和羧基二羟鬼笔毒肽(phallacidin)。
语种:
中文
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A static pressure sensitive receptor APJ promote H9c2 cardiomyocyte hypertrophy via PI3K-autophagy pathway
作者:
Xie, Feng;Liu, Wei;Feng, Fen;Li, Xin;Yang, Li;...
期刊:
生物化学与生物物理学报 ,2014年46(8):699-708 ISSN:1672-9145
通讯作者:
Li, Lanfang
作者机构:
[Xie, Feng; Liu, Wei; Li, Lanfang; Lv, Deguan; Qin, Xuping; Yang, Li; Feng, Fen; Li, Xin; Chen, Linxi] Univ South China, Inst Pharm & Pharmacol, Learning Key Lab Pharmacoprote, Hengyang 421001, Peoples R China.;[Liu, Wei] Cent S Univ, Dept Pharm, Xiangya Hosp 3, Changsha 410013, Hunan, Peoples R China.
通讯机构:
[Li, Lanfang] U;Univ South China, Inst Pharm & Pharmacol, Learning Key Lab Pharmacoprote, Hengyang 421001, Peoples R China.
关键词:
apelin;static pressure;APJ;hypertrophy;PI3K;autophagy
摘要:
This study is designed to investigate whether APJ receptor acts as a sensor in static pressure-induced cardiomyocyte hypertrophy and to investigate the mechanism of PI3K-autophagy pathway. The left ventricular hypertrophy rat model was established by coarctation of abdominal aorta. H9c2 rat cardiomyocytes were cultured in the presence of static pressure which was given by a custom-made pressure incubator. The results revealed that the expression of apelin/APJ system, PI3K, Akt and their phosphorylation were significantly increased in the operation group. Static pressure up-regulated the APJ expression, PI3K phosphorylation, Akt phosphorylation, LC3-II/I and beclin-1 expression in cardiomyocytes. APJ shRNA pGPU6/Neo-rat-399, PI3K inhibitor LY294002, Akt inhibitor 1701-1 blocked the up-regulation of APJ, PI3K phosphorylation, Akt phosphorylation, LC3-II/I and beclin-1 expression, respectively. Moreover, static pressure increased the diameter, volume, protein content of cells, and these could be reversed when the cells were treated with pGPU6/Neo-rat-399, LY294002, and autophagy inhibitor 3-methyladenine, respectively. These results suggested that static pressure up-regulates APJ expression to promote cardiomyocyte hypertrophy by a PI3K-autophagy pathway.
语种:
英文
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ELABELA: a novel hormone in cardiac development acting as a new endogenous ligand for the APJ receptor
作者:
Xie, Feng;Lv, Deguan;Chen, Linxi
期刊:
生物化学与生物物理学报 ,2014年46(7):620-622 ISSN:1672-9145
通讯作者:
Linxi Chen *
作者机构:
[Xie, Feng; Lv, Deguan] Learning Key Laboratory for Pharmaco-proteomics, Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, China;[Chen, Linxi] Learning Key Laboratory for Pharmaco-proteomics, Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, China lxchen6@126.com
通讯机构:
[Chen, L.] L;Learning Key Laboratory for Pharmaco-proteomics, Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, China
关键词:
hormones;cardiac development
摘要:
Human ELA consists of three exons on chromosome 4, which generates a transcript (AK092578) that is annotated as a non-coding RNA. However, Chng et al. [1] has found that this gene contains a conserved open reading frame predicted to express a conserved vertebrate protein of 54 amino acids (aa) consisting of a secretory signal and a mature 32-aa peptide, which was called as ELABELA (ELA). The sequence of human mature ELA is Gln-Arg-Pro-Val-Asn-Leu-Thr-Met-Arg-Arg-Lys-Leu-Arg-Lys-His-Asn-Cys-Leu-Gln-Arg-Arg-Cys-Met-Pro-Leu-His-Ser-Arg-Val-Pro-Phe-Pro. Phylogenetic analysis revealed that the 32-aa mature peptide is evolutionarily highly conserved, with the last 13 residues being nearly invariant in all vertebrate species. ELA has also been previously reported to be highly expressed in undifferentiated human embryonic stem cells (hESCs) and be sharply down-regulated during differentiation [2]. Chng et al. [1] used an allelic series of zebrafish ELA mutants to show that ELA deficiency leads to severe defects in cardiac morphogenesis and often results in the complete absence of a heart. ELA mutant displayed specific defects in the mesen-dodermal lineage during gastrulation, as observed by the reduction of gata5 and sox17 expression. Taking together, these results suggested that ELA plays a role in the regulation of heart development. Whereas till now, no hormonal peptides has been reported to be involved in early development, particularly in the formation of the three embryonic germ layers. Chng et al. [1] first discovered an endogenous peptide hormone with potent embryonic signaling activity, which has great prospects in therapeutic applications such as heart repair and gene therapy in development.
语种:
英文
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安徽野菊花挥发油化学成分分析
作者:
何小珍;蒋军辉;赵雷;徐小娜
期刊:
应用化工 ,2014年43(8):1536-1539,1546 ISSN:1671-3206
作者机构:
[何小珍] 南华大学药学与生命科学院;[蒋军辉] 南华大学化学化工学院;[赵雷; 徐小娜] 南华大学公共卫生学院
关键词:
野菊花;挥发油;气相色谱-质谱联用;直观推导式演进特征投影法
摘要:
采用水蒸气蒸馏法提取野菊花挥发油,利用气相色谱-质谱法( GC-MS)测定挥发油化学成分,同时结合直观推导式演进特征投影法解析重叠色谱峰,并采用总体积积分法进行定量。鉴定出66种化合物,占总含量的75.32%,主要成分为石竹烯氧化物、匙叶桉油烯醇、6-isopropenyl-4,8a-dimethyl-1,2,3,5,6,7,8,8a-octahydro-naph-thalen-2-ol和反式长松香芹醇。安徽产野菊花挥发油的化学成分主要为单萜烯类、倍半萜烯类及其含氧衍生物等。采用GC-MS 联用技术结合直观推导式演进特征投影法分析安徽产野菊花挥发油,提高了定性定量结果的准确性。
语种:
中文
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金樱子总黄酮体外抗氧化活性的研究
作者:
文红波;曹运长;吴玉兰;张秋菊;冷超群
期刊:
天然产物研究与开发 ,2014年26(7):1127-1131 ISSN:1001-6880
作者机构:
[文红波; 曹运长; 吴玉兰; 张秋菊; 冷超群] 南华大学药学与生物科学学院,生化与分子生物学教研室
关键词:
金樱子;总黄酮;自由基;清除作用;抗氧化酶
摘要:
本文研究了金樱子总黄酮(RLTF)体外清除自由基的能力,并观察其对过氧化氢诱导损伤的人脐静脉内皮细胞(HUVEC)抗氧化作用的影响。采用邻苯三酚自氧化法、水杨酸法、DPPH 法分别测定RLTF 对超氧阴离子自由基(O_2~-·)、羟自由基(·OH)、二苯代苦味酰基自由基(DPPH·)的清除率; Hoechst 染色法分析各组细胞凋亡情况; MTT 法测定细胞活力; 分光光度法检测各组细胞中SOD、CAT、GSH-Px 活性。结果显示,在20 ~ 200μg 范围内,随着样品加入量增大,RLTF 对·OH 和DPPH·的清除率逐渐增大,而对O_2~-·的清除率则是先增大后减少; RLTF 对O_2~-·的清除作用偏低,对·OH 的清除率与芦丁相当,对DPPH·的清除能力则强于芦丁而弱于 Vc。Hoechst 染色观察结果表明RLTF 预处理细胞后,能明显提高细胞的抗凋亡作用。与H_2O_2损伤组比较,不同浓度RLTF 预处理组的细胞活力均显著升高(P < 0. 01); 不同浓度RLTF 预处理组细胞中的SOD、CAT 和 GSH-Px 活性均明显增加(P < 0. 01),且呈剂量依赖性。上述结果表明,RLTF 具有较强的自由基体外清除能力,能有效提高氧化损伤HUVEC 细胞中SOD、CAT、GSH-Px 的酶活性,其可能通过清除细胞内自由基和提高细胞抗氧化酶活性来提高细胞的抗氧化活性。
语种:
中文
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二烯丙基二硫活化NADPH氧化酶诱导人白血病K562细胞凋亡
作者:
易岚;伍尤华;谭晖;何洁;李林蔚;...
期刊:
中国药理学通报 ,2014年30(8):1107-1112 ISSN:1001-1978
通讯作者:
Yi, L.
作者机构:
[苏琦; 谭晖; 何洁; Shan J.] Cancer Research Institute, First Affiliated Hospital, University of South China, Hengyang Hunan, 421001, China;[李林蔚] College of Pharmacy and Biological Sciences, First Affiliated Hospital, University of South China, Hengyang Hunan, 421001, China;[伍尤华] Dept of Oncology, First Affiliated Hospital, University of South China, Hengyang Hunan, 421001, China;[易岚] Cancer Research Institute, First Affiliated Hospital, University of South China, Hengyang Hunan, 421001, China, College of Pharmacy and Biological Sciences, First Affiliated Hospital, University of South China, Hengyang Hunan, 421001, China
通讯机构:
[Yi, L.] C;Cancer Research Institute, First Affiliated Hospital, University of South China, Hengyang Hunan, China
关键词:
NADPH氧化酶;活性氧;凋亡;人白血病;K562细胞
摘要:
目的研究DADS诱导人白血病K562细胞凋亡的分子机制。方法应用MTT法检测细胞的活性;流式细胞术检测细胞内的活性氧(reactive oxygen species,ROS)水平以及凋亡细胞百分率;Real-time PCR检测NADPH氧化酶各亚基mRNA水平;免疫共沉淀检测蛋白Rac2与蛋白p67phox的结合;Western blot检测Rac2蛋白的表达。结果DADS能明显抑制K562细胞的增殖,呈时间和剂量依赖性;6 mg·L~(-1) DADS作用人白血病K562细胞6 h后,NADPH氧化酶复合物的6个亚基mRNA水平都明显上调;5.0、10.0 mg?L~(-1) DADS作用人白血病K562细胞24 h后蛋白Rac2的表达水平明显上调;免疫共沉淀结果显示,DADS诱导的K562细胞凋亡过程中有Rac2与p67phox结合;流式细胞术检测凋亡细胞百分率结果显示,PMA能明显提高DADS诱导K562细胞凋亡的作用,而DPI能抑制DADS诱导K562细胞凋亡。PMA能提高DADS诱导K562细胞活性氧的水平,而DPI明显抑制了活性氧的产生。结论NADPH氧化酶的活化是DADS诱导K562细胞凋亡过程中活性氧的主要来源,DADS通过活化NADPH氧化酶诱导K562细胞凋亡。
语种:
中文
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RNA修复研究进展
作者:
Li Wei;Su Ze-Hong;Yang Jie;He Shu-Ya*
期刊:
生物化学与生物物理进展 ,2014年41(6):525-531 ISSN:1000-3282
通讯作者:
He Shu-Ya
作者机构:
[Yang Jie; Su Ze-Hong; He Shu-Ya; Li Wei] Univ South China, Inst Biochem & Mol Biol, Hengyang 421001, Peoples R China.
通讯机构:
[He Shu-Ya] U;Univ South China, Inst Biochem & Mol Biol, Hengyang 421001, Peoples R China.
关键词:
RNA损伤;RNA修复
摘要:
机体DNA损伤修复的机制目前已研究得比较全面,而RNA损伤修复的研究却没有引起广泛的认识.主要由于人们长期以来认为损伤的RNA会被机体特异性降解而不是修复.近年来,随着多个RNA损伤修复系统的相继发现,揭示机体对损伤RNA可能优先选择进行修复.本文从噬菌体型RNA修复系统、细菌型RNA修复系统、酵母型RNA修复系统和人类的RNA损伤修复系统四个方面对目前RNA损伤修复研究的最新进展做一综述.
语种:
中文
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GW501516通过TGFβ-Smad3信号途径促进人脐静脉内皮细胞PAI-1的表达
作者:
Mo Zhong-Cheng;Li Xia;Zhang Da-Di;Zhu Ming-Yan;Zhang Qing-Hai;...
期刊:
生物化学与生物物理进展 ,2014年41(7):674-681 ISSN:1000-3282
通讯作者:
Yi Guang-Hui
作者机构:
[Lu Yan-Ju; Zhang Da-Di; Zeng Ying; Wu Rong; Mo Zhong-Cheng; Chen Yi; Li Xia; Zhu Ming-Yan; Zhang Qing-Hai; Yi Guang-Hui] Univ South China, Inst Cardiovasc Res, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Peoples R China.
通讯机构:
[Yi Guang-Hui] U;Univ South China, Inst Cardiovasc Res, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Peoples R China.
关键词:
纤溶酶原激活物抑制剂1;过氧化物酶体增殖物激活型受体δ;转化生长因子β
摘要:
为探讨过氧化物酶体增殖物激活型受体δ(peroxisome proliferator- activated receptor-δ,PPARδ)激动剂GW501516对人脐静脉内皮细胞纤溶酶原激活物抑制剂1 (PAI-1)表达的影响及机制,采用siRNA、TGFβ-Smad3信号通路阻滞剂等处理细胞,经实时定量PCR、Western blot 方法分别检测细胞中PAI-1及磷酸化Smad3蛋白的表达.结果显示,与对照组比较, GW501516可诱导人脐静脉内皮细胞(HUVEC)中PAI-1表达,且此效应呈浓度和时间依赖性(P < 0.05);siRNA 沉默PPARδ的表达后,可阻抑GW501516对HUVEC细胞PAI-1表达的促进作用;TGFβ-Smad3信号通路抑制剂SB-431542与SIS3均可降低HUVEC细胞pSmad3蛋白的表达,而细胞PAI-1表达也随之降低.结果提示,GW501516可促进HUVEC细胞PAI-1的表达,其机制可能与TGFβ-Smad3信号通路有关.
语种:
中文
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讨论式教学法在卫生微生物学理论教学中的运用
作者:
封少龙;曹朝晖;胡小波;龙石银
期刊:
中国病原生物学杂志 ,2014年9(7):I0001-I0003 ISSN:1673-5234
作者机构:
[封少龙] 南华大学公共卫生学院;[曹朝晖; 胡小波; 龙石银] 南华大学药学与生物科学学院
关键词:
讨论式教学法;教学模式;卫生微生物学
摘要:
研究讨论式教学法在卫生微生物学理论教学中的运用,从该教学法运用的必要性、组织与实施及存在的问题与解决办法等方面探讨卫生微生物学课程理论教学改革的基本思路。通过积极探索课程教学新方法、新手段,充分发挥学生主体作用,提高教学质量并提升学生综合素养,以适应我国社会发展对卫生检验高级人才的需求。
语种:
中文
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Neu - P11下调JNK及其磷酸化水平改善睡眠限制大鼠葡萄糖稳态
作者:
佘美华;杨升华;尹卫东
期刊:
中国动脉硬化杂志 ,2014年22(9):881-884 ISSN:1007-3949
作者机构:
[佘美华; 尹卫东] 南华大学药学与生物科学学院生物技术系;[杨升华] 浙江大学医学院附属第一医院;[Moshe Laudon] Drug Discovery,Neurim Pharmaceuticals lad
关键词:
褪黑素;睡眠限制;胰岛素敏感性
摘要:
目的研究新型褪黑素(MLT)受体激动剂 Neu-P11对睡眠限制大鼠胰岛素敏感性的影响及其作用机制。方法采用转笼法对SD大鼠进行8天的睡眠限制,期间每天分别给予腹腔注射 Neu-P11 (20 mg / kg)、MLT (5 mg / kg)、生理盐水。实验末测定空腹血糖、胰岛素、丙二醛(MDA)水平及GSH-Px、SOD 活性,检测骨骼肌组织 JNK 及其磷酸化水平。结果与正常对照组比较,睡眠限制大鼠空腹血糖、胰岛素、HOMA-IR 及 MDA 水平明显升高,SOD、GSH-Px 活性降低,JNK 及其磷酸化水平显著增高;而 Neu-P11、MLT 处理的睡眠限制鼠血糖、胰岛素、MDA、JNK 及其磷酸化水平均下降,同时 SOD、GSH-Px 活性增强。提示 Neu-P11、MLT 可以改善睡眠限制鼠的抗氧化能力和葡萄糖稳态。结论Neu-P11通过下调 JNK 及其磷酸化水平而改善其抗氧化能力,从而改善睡眠限制引起的胰岛素抵抗。
语种:
中文
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Synthesis of New 7-O-Modified Chrysin Derivatives and Their Anti-proliferative and Apoptotic Effects on Human Gastric Carcinoma MGC-803 Cells
作者:
Liu Yunmei* ;Song Xiudao;Ma Jin;He Jun;Zheng Xing;...
期刊:
高等学校化学研究:英文版 ,2014年30(6):925-930 ISSN:1005-9040
通讯作者:
Liu Yunmei
作者机构:
[Liu Yunmei; Zhao Zihao; Song Xiudao; Zheng Xing; Lei Xiaoyong; Pan Xia] Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.;[Jiang Guorong; Song Xiudao] Suzhou Hosp Tradit Chinese Med, Suzhou Acad Wumen Chinese Med, Suzhou 215003, Peoples R China.;[Ma Jin] Soochow Univ, Affiliated Childrens Hosp, Suzhou 215003, Peoples R China.;[He Jun] Univ South China, Inst Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构:
[Liu Yunmei] U;Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.
关键词:
7-O-Modified chrysin;Anti-proliferative effect;Apoptotic effect;Flavanoid
摘要:
Two series of 7-O-modified chrysin derivatives were prepared from 7-O-carboxymethyl chrysin(2a), 7-O-carboxypropylchrysin(2b) and short-chain alcohols by using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride(EDCI), N-hydroxybenzotriazole(HOBt) and 4-dimethylamiopryidine(DMAP) as coupling reagents. Taking cisplatin as a reference substance, their anti-proliferative activities in vitro against human gastric carcinoma MGC-803 cells were evaluated by the standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide(MTT) method. The results showed that among the compounds tested, compound hepty 4-(5-hydroxy-4-oxo-2-phenyl-4H-chromen-7-yloxy) acetate(3f) displayed the most potent growth-inhibitory effect on MGC-803 cells with half maximal inhibitory concentration(IC50) value of 3.23 μmol/L. The preliminary mechanism of inhibitory effect of compound 3f was also detected by flow cytometry(FCM), and the compound exerted anticancer activity via inducing the apoptosis of MGC-803 cells in a dose dependent manner, which suggested that compound 3f would be a potential anti-cancer agent. © 2014, Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH.
语种:
英文
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脂蛋白脂酶与糖尿病心肌病
作者:
刘翔宇;尹卫东;唐朝克
期刊:
生理科学进展 ,2014年45(1):16-20 ISSN:0559-7765
作者机构:
[刘翔宇; 尹卫东; 唐朝克] 南华大学心血管病研究所
关键词:
糖尿病;心肌
摘要:
脂蛋白脂酶(lipoprotein lipase,LPL)通过水解血浆中富含甘油三酯( triglyceride,TG)的脂蛋白,为心肌组织提供游离脂肪酸(free fatty acid,FFA)供能。糖尿病期间,由于心肌组织减弱对葡萄糖的利用能力,导致心脏供能不足。此时,机体通过一系列机制上调心肌LPL 活性,促进血浆极低密度脂蛋白(very low density lipoprotein,VLDL)和乳糜微粒(chylomicrons,CM)的水解,以增强FFA 为心肌组织代偿性供能。糖尿病患者通过上调心肌LPL 活性,进而促使血浆FFA 浓度显著升高,导致大量活性氧、脂质等在心肌细胞内蓄积,并潜在地诱发糖尿病心肌病( diabetic cardiomyopathy,DCM) 。因此,本文主要针对糖尿病对心肌LPL 的调控机制及LPL 如何潜在地诱发DCM 做—综述,以期为DCM 提供新的治疗靶点和途径。
语种:
中文
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大孔吸附树脂联合葡聚糖凝胶Sephadex LH20分离制备鹅膏肽类毒素的研究
作者:
胡劲松;陈作红
期刊:
菌物学报 ,2014年33(3):549-559 ISSN:1672-6472
作者机构:
湖南师范大学生命科学学院 湖南长沙410081;南华大学生物研究所 湖南衡阳421001;[胡劲松; 陈作红] 湖南师范大学
关键词:
大孔吸附树脂;高效液相色谱;鹅膏毒肽;鬼笔毒肽
摘要:
鹅膏菌肽类毒素是蘑菇中毒导致死亡的最主要因素,同时也是生物学研究领域的一种重要工具试剂,但由于其结构相似,分离制备单体化合物比较困难。通过4种大孔吸附树脂对致命鹅膏Amanita exitialis 子实体中主要肽类毒素组分的静态吸附与解吸附性能比较,结果表明XAD16的吸附能力最强,同时也有较强的解吸附能力,但XAD16柱层析梯度洗脱时对毒素分离效果差,表明XAD16只具有吸附富集鹅膏肽类毒素的特性但不适于分离。经XAD16柱层析富集的毒素成分,通过葡聚糖凝胶sephadex LH20柱层析分离,结果表明sephadex LH20柱层析对鹅膏肽类毒素具有较好的分离效果,经过2次sephadex LH20柱层析,获得了5个纯度达50%–80%的鹅膏肽类毒素单体化合物,进一步利用半制备高效液相色谱系统(HPLC)纯化,分离得到了7个纯度达95%以上的鹅膏肽类毒素单体,其中5个经质谱分析鉴定为:α-鹅膏毒肽(α-amanitin)、β-鹅膏毒肽(β-amanitin)、脱氧二羟毒伞素(desoxoviroidin)、羧基三羟鬼笔毒肽(phallisacin)和羧基二羟鬼笔毒肽(phallacidin)。
语种:
中文
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Hydrogen sulfide inhibits homocysteine-induced endoplasmic reticulum stress and neuronal apoptosis in rat hippocampus via upregulation of the BDNF-TrkB pathway
作者:
Wei, Hai-jun;Xu, Jin-hua;Li, Man-hong;Tang, Ji-ping;Zou, Wei;...
期刊:
中国药理学报 ,2014年35(6):707-715 ISSN:1671-4083
通讯作者:
Zhang, Ping
作者机构:
[Wei, Hai-jun; Tang, Ji-ping; Tang, Xiao-qing; Li, Man-hong] Univ South China, Coll Med, Inst Neurosci, Hengyang 421001, Peoples R China.;[Xu, Jin-hua] Univ South China, Lab Ctr Biochem & Mol Biol, Hengyang 421001, Peoples R China.;[Tang, Ji-ping; Zou, Wei; Li, Man-hong; Zhang, Ping] Univ South China, Nanhua Affiliated Hosp, Dept Neurol, Hengyang 421001, Peoples R China.;[Wang, Li] Univ South China, Coll Med, Dept Anthropot, Hengyang 421001, Peoples R China.;[Wang, Chun-yan] Univ South China, Coll Med, Dept Pathophysiol, Hengyang 421001, Peoples R China.
通讯机构:
[Zhang, Ping] U;Univ South China, Nanhua Affiliated Hosp, Dept Neurol, Hengyang 421001, Peoples R China.
关键词:
hydrogen sulfide;homocysteine;neurotoxicity;hippocampus;apoptosis;ER stress;BDNF;tyrosine protein kinase B;Alzheimer's disease
摘要:
Aim:Homocysteine (Hcy) can elicit neuronal cell death, and hyperhomocysteinemia is a strong independent risk factor for Alzheimer's disease. The aim of this study was to examine the effects of hydrogen sulfide (H 2 S) on Hcy-induced endoplasmic reticulum (ER) stress and neuronal apoptosis in rat hippocampus.Methods:Adult male SD rats were intracerebroventricularly (icv) injected with Hcy (0.6 μmol/d) for 7 d. Before Hcy injection, the rats were treated with NaHS (30 or 100 μmol·kg -1 ·d -1, ip) and/or k252a (1 μg/d, icv) for 2 d. The apoptotic neurons were detected in hippocampal coronal slices with TUNEL staining. The expression of glucose regulated protein 78 (GRP78), C/EBP homologous protein (CHOP), cleaved caspase-12, and BDNF in the hippocampus were examined using Western blotting assays. The generation of H 2 S in the hippocampus was measured with the NNDPD method.Results:Hcy markedly inhibited the production of endogenous H 2 S and increased apoptotic neurons in the hippocampus. Furthermore, Hcy induced ER stress responses in the hippocampus, as indicated by the upregulation of GRP78, CHOP, and cleaved caspase-12. Treatment with the H 2 S donor NaHS increased the endogenous H 2 S production and BDNF expression in a dose-dependent manner, and significantly reduced Hcy-induced neuronal apoptosis and ER stress responses in the hippocampus. Treatment with k252a, a specific inhibitor of TrkB (the receptor of BDNF), abolished the protective effects of NaHS against Hcy-induced ER stress in the hippocampus.Conclusion:H 2 S attenuates ER stress and neuronal apoptosis in the hippocampus of Hcy-treated rats via upregulating the BDNF-TrkB pathway. © 2014 CPS and SIMM All rights reserved.
语种:
英文
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Periostin过表达对鼻咽癌6-10B细胞侵袭和迁移的影响及机制
作者:
王惠洁;石金凤;谢远杰;曾谷清;杜雅兰;...
期刊:
解剖学报 ,2014年45(4):500-506 ISSN:0529-1356
通讯作者:
Li, M.-X.
作者机构:
Department of Histology and Embryology, School of Medicine, University of South China, Hu'nan Hengyang, 421001, China;Laboratory of Cell Differentiation and Apoptosis, School of Medicine, University of South China, Hu'nan Hengyang, 421001, China;[Zeng G.-Q.] Nursing Research Institute, School of Life Science and Technology, University of South China, Hu'nan Hengyang, 421001, China;[庾江东] Otolaryngology Department, Hengyang Central Hospital, Hu'nan Hengyang, 421001, China;[黄欣琼; 龙治峰; 王惠洁; 石金凤; 杜雅兰; 谢远杰; 李美香] Department of Histology and Embryology, School of Medicine, University of South China, Hu'nan Hengyang, 421001, China, Laboratory of Cell Differentiation and Apoptosis, School of Medicine, University of South China, Hu'nan Hengyang, 421001, China
通讯机构:
[Li, M.-X.] L;Laboratory of Cell Differentiation and Apoptosis, School of Medicine, University of South China, Hu'nan Hengyang, China
关键词:
鼻咽癌;转移;转染;免疫组织化学;人
摘要:
目的探讨外源表达periostin蛋白对鼻咽癌(NPC)6-10B细胞侵袭和迁移能力的影响及机制。方法采用脂质体转染技术建立稳定高表达periostin的6-10B细胞,RT-PCR结合Western blotting检测转染效率,Tanswell分析periostin高表达后6-10B细胞侵袭和迁移能力的改变,明胶酶谱法检测基质金属蛋白酶(MMPs)活性的变化;免疫组织化学检测整合素(integrin)α_vβ_5在转染periostin前后6-10B细胞中的表达以及其与periostin在鼻咽癌和正常鼻咽黏膜组织中的表达, image-pro Plus 6.0软件进行图像分析及吸光度测定,统计学分析鼻咽癌组织中periostin和integrinα_vβ_5表达强度的相关性。结果 Periostin过表达的6-10B细胞侵袭、迁移能力明显增强,细胞培养上清中MMP-2、MMP-9活性增加,integrin α_vβ_5在periostin过表达的6-10B细胞及NPC组织的表达明显强于对照组,Spearman相关性分析显示, integrin α_vβ_5和 periostin 在 NPC 组织中的表达强度成正相关(r =0.682, P =0.000)。结论外源表达periostin能促进NPC的侵袭和转移,其机制可能是通过与NPC细胞膜上integrinα_vβ_5受体结合而提高MMPs的活性。
语种:
中文
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Apolipoprotein A-I inhibits LPS-induced atherosclerosis in ApoE~(–/–) mice possibly via activated STAT3-mediated upregulation of tristetraprolin
作者:
Yin, Kai;Tang, Shi-lin;Yu, Xiao-hua;Tu, Guang-hui;He, Rong-fang;...
期刊:
中国药理学报 ,2013年34(6):837-846 ISSN:1671-4083
通讯作者:
Tu, Jian
作者机构:
[Tang, Chao-ke; Jiang, Zhi-sheng; Yu, Xiao-hua; Yin, Kai; Xie, Di; Li, Jin-feng; Tang, Shi-lin] Univ South China, Life Sci Res Ctr, Key Lab Atherosclerol Hunan Prov, Inst Cardiovasc Res, Hengyang 421001, Peoples R China.;[Gui, Qing-jun; Yin, Kai; Xie, Di] Univ South China, Coll Med, Dept Diagnost, Hengyang 421001, Peoples R China.;[Tang, Shi-lin] Univ South China, Affiliated Hosp 1, Dept Intens Care Unit, Hengyang 421001, Peoples R China.;[Tu, Guang-hui] Nan Xian Peoples Hosp, Dept Pathol, Yiyang 413200, Peoples R China.;[He, Rong-fang] Univ South China, Affiliated Hosp 1, Dept Pathol, Hengyang 421001, Peoples R China.
通讯机构:
[Tu, Jian] U;Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.
关键词:
apolipoprotein A-I;heart;atherosclerosis;inflammation;cytokines;JAK2/STAT3 signaling pathway;tristetraprolin;lipopolysaccharide;ApoE-/-mice;macrophage;apolipoprotein A-I;heart;atherosclerosis;inflammation;cytokines;JAK2/STAT3 signaling pathway;tristetraprolin;lipopolysaccharide;ApoE-/-mice;macrophage
摘要:
Aim:To investigate the effects of the major component of high-density lipoprotein apolipoprotein A-I (apoA-I) on the development of atherosclerosis in LPS-challenged ApoE -/- mice and the underlying mechanisms.Methods: Male ApoE-KO mice were daily injected with LPS (25 μg, sc) or PBS for 4 weeks. The LPS-challenged mice were intravenously injected with rAAV-apoA-I-GFP or rAAV-GFP. After the animals were killed, blood, livers and aortas were collected for biochemical and histological analyses. For ex vivo experiments, the abdominal cavity macrophages were harvested from each treatment group of mice, and cultured with autologous serum, then treated with LPS.Results:Chronic administration of LPS in ApoE -/- mice significantly increased the expression of inflammatory cytokines (TNF-α, IL-1β, IL-6, and MCP-1), increased infiltration of inflammatory cells, and enhanced the development of atherosclerosis. In LPS-challenged mice injected with rAAV-apoA-I-GFP, viral particles and human apoA-I were detected in the livers, total plasma human apoA-I levels were grammatically increased; HDL-cholesterol level was significantly increased, TG and TC were slightly increased. Furthermore, overexpression of apoA-I significantly suppressed the expression of proinflammatory cytokines, reduced the infiltration of inflammatory cells, and decreased the extent of atherosclerotic lesions. Moreover, overexpression of apoA-I significantly increased the expression of the cytokine mRNA-destabilizing protein tristetraprolin (TTP), and phosphorylation of JAK2 and STAT3 in aortas. In ex vivo mouse macrophages, the serum from mice overexpressing apoA-I significantly increased the expression of TTP, accompanied by accelerated decay of mRNAs of the inflammatory cytokines.Conclusion:ApoA-I potently suppresses LPS-induced atherosclerosis by inhibiting the inflammatory response possibly via activation of STAT3 and upregulation of TTP. © 2013 CPS and SIMM.
语种:
英文
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抑制T细胞增殖的抗CD98重链单克隆抗体的建立
作者:
练高建;吴端生;苏泽红;安友康二
期刊:
中国实验动物学报 ,2013年21(2):64-67 ISSN:1005-4847
作者机构:
南华大学实验动物学部,湖南衡阳,421001;南华大学药学与生命科学学院,湖南衡阳,421001;日本德岛大学大学院免疫学教研室,日本德岛770-8503;[练高建; 吴端生; 苏泽红] 南华大学;[安友康二] 德岛大学
关键词:
CD98重链;单克隆抗体;抑制;T细胞增殖
摘要:
目的寻找能调节T细胞功能的相关分子,进行与T细胞介导的自身免疫性疾病相关的研究。方法收集BALB/c小鼠脾细胞,免疫Wistar大鼠,进行细胞融合,建立杂交瘤细胞系。筛选得到43株能调节T细胞功能的杂交瘤细胞系,对其中一株最能抑制T细胞增殖的杂交瘤细胞系进行了进一步的深入研究。结果显示其目标分子是CD98重链,同时后续实验显示抗CD98单克隆抗体能抑制纤连蛋白介导的细胞分布,但不影响氨基酸转运。而且混合淋巴细胞反应显示该抗体能显著抑制T细胞增殖反应。结论抗CD98单克隆抗体能有效抑制T细胞增殖,有望将本抗体用于T细胞介导的自身免疫性疾病的相关预防及治疗中。
语种:
中文
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ABCA1的胞内运输及功能研究新进展
作者:
Tang Yan-Yan;Chen Wu-Jun;Lu Qian;Tang Chao-Ke*
期刊:
生物化学与生物物理进展 ,2013年40(6):510-519 ISSN:1000-3282
通讯作者:
Tang Chao-Ke
作者机构:
[Chen Wu-Jun; Lu Qian; Tang Yan-Yan; Tang Chao-Ke] Univ South China, Inst Cardiovasc Dis, Key Lab Atherosclerol Hunan Prov, Life Sci Res Ctr, Hengyang 421001, Peoples R China.;[Chen Wu-Jun] Univ South China, Insitute Pharm & Pharmacol, Hengyang 421001, Peoples R China.
通讯机构:
[Tang Chao-Ke] U;Univ South China, Inst Cardiovasc Dis, Key Lab Atherosclerol Hunan Prov, Life Sci Res Ctr, Hengyang 421001, Peoples R China.
关键词:
三磷酸腺苷结合盒转运体A1(ABCA1);胞内运输;胆固醇流出;免疫;炎症
摘要:
三磷酸腺苷结合盒转运体A1(ABCA1)具有介导细胞内脂质流出,维持细胞脂质稳态的功能.新生的ABCA1必须经过胞内运输和各种化学修饰等过程,最终成为具有功能的成熟转运体,才能行使其转运脂质的功能,因此,ABCA1在胞内的运输过程和正确质膜定位对其介导胆固醇流出的功能至关重要.目前ABCA1相关研究主要集中于脂质转运方面,并提出各种胆固醇流出机制的模型,如通道转运模型、蘑菇状突起模型和胞吞-胞吐转运模型等.最近研究显示,ABCA1还具有调节质膜脂筏结构、参与免疫和炎症调节等新功能.本文主要针对ABCA1的胞内运输过程以及各种功能做一综述,以期为动脉粥样硬化相关疾病提供新的治疗靶点和途径.
语种:
中文
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