期刊论文

Isolation and preparation of cyclopeptide toxins by macroporous adsorptive resins combined with sephadex LH20 column chromatography from Amanita exitialis

中文

菌物学报

Mycosystema

1672-6472

2014

33

3

549-559

10.13346/j.mycosystema.140075

国家自然科学基金(No.31372118,30972073)；

本校为其他机构

鹅膏菌肽类毒素是蘑菇中毒导致死亡的最主要因素,同时也是生物学研究领域的一种重要工具试剂,但由于其结构相似,分离制备单体化合物比较困难。通过4种大孔吸附树脂对致命鹅膏Amanita exitialis 子实体中主要肽类毒素组分的静态吸附与解吸附性能比较,结果表明XAD16的吸附能力最强,同时也有较强的解吸附能力,但XAD16柱层析梯度洗脱时对毒素分离效果差,表明XAD16只具有吸附富集鹅膏肽类毒素的特性但不适于分离。经XAD16柱层析富集的毒素成分,通过葡聚糖凝胶sephadex LH20柱层析分离,结果表明sephadex LH20柱层析对鹅膏肽类毒素具有较好的分离效果,经过2次sephadex LH20柱层析,获得了5个纯度达50%–...

The cyclopeptide toxins are mainly responsible for the death of humans in the mushroom poisoning, and these toxins are also the important tools in the biology research field. It is difficult to separate the morphon toxins from the fruiting bodies of Amanito species due to their similar structures. The static adsorption and desorption properties of four macroporous adsorptive resins on the cyclopeptide toxins of Amanita exitialis were compared and analyzed in this study The results showed that XAD16 possessed the highest static adsorption proper...