摘要：
INTRODUCTION: Essential Tremor (ET) is one of the most common movement disorders. Oral drugs play a crucial role in treating ET, with various available options such as propranolol, primidone and topiramate. However, the medication status and related factors among Chinese ET patients are unknown yet. METHODS: This study used the baseline data from the National Survey of Essential Tremor Plus in China cohort. ET patients with information related to medication intake were included. Medication patients was defined as patients who were taking medication at the time of the survey. We further defined recommended medication users according to Chinese guideline recommendations and clinical knowledge. We used mean and standard deviation (SD), median and interquartile range (IQR), or frequencies and percentages when appropriate for descriptive analysis. We used multivariate logistic regression analyses to explore factors related to medication intake in all ET patients, and in recommended medication users respectively. RESULTS: Of 1153 included ET participants, 207 (18.0%) took medication. Arotinolol (115, 55.6%) and propranolol (63, 30.4%) were the top 2 used medicines. Patients with middle school education (odds ratio 0.57, 95% confidence interval 0.39 to 0.83), college or higher level education (0.46, 0.28 to 0.76) and late onset ET (LO-ET) (0.38, 0.23 to 0.63) were less likely to take medication. Patients with intention tremor (1.90, 1.38 to 2.62), every 10-unit increase in age (1.10, 1.00 to 1.21), Tremor Research Group Essential Tremor Rating Assessment Scale (TETRAS) Part 1 (1.63, 1.37 to 1.93), and TETRAS Part 2 (1.81, 1.48 to 2.22) were more likely to take medication. Among 332 recommended medication users, only 104 (31.3%) took medicine. The associations of LO-ET (0.36, 0.17 to 0.75), intention tremor (2.27, 1.35 to 3.81), TETRAS Part1 (1.52, 1.09 to 2.13) and TETRAS Part 2 (1.59, 1.15 to 2.20) with medication were similar to all ET patients. CONCLUSION: The proportion of medication intake is low among both all ET patients and recommended medication users. The top two commonly used medications among all ET patients are arotinolol and propranolol. Influencing factors of medication intake are different between all ET patients and recommended medication users. Clinicians are suggested to provide counseling and education on ET medication to promote medication intake.
作者机构：
[Xiao, Hao; Wu, Yinyin; Fu, Qian; Tang, Li; Li, Na; Li, Zelong; Liu, Fen; Tan, Xiaofeng; Zhou, Wei; Wang, Wenjie; Lin, Nanyun; Wang, Feirong; Wang, Cheng-kun; Yang, Qinglai; Kang, Qiang; Tan, XF; Wu, Gui-long; Yang, Sha] Univ South China, Ctr Mol Imaging Probe, Hunan Prov Key Lab Tumor Cellular & Mol Pathol, Canc Res Inst,Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Chen, Guodong; Yang, Qinglai] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Hepatopancreatobiliary Surg, Hengyang 421001, Hunan, Peoples R China.;[Tan, Xiaofeng; Yang, Qinglai; Tan, XF] Hunan Prov Maternal & Child Hlth Care Hosp, Key Lab Birth Defect Res & Prevent, Natl Hlth Commiss, Changsha 410008, Hunan, Peoples R China.;[Tang, Li] Hainan Normal Univ, Coll Chem & Chem Engn, Key Lab Trop Med Plant Chem, Minist Educ, Haikou 571158, Hainan, Peoples R China.;[Tan, Xiaofeng; Yang, Qinglai; Tan, XF] Univ South China, Hengyang Med Sch, MOE Key Lab Rare Pediat Dis, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Tan, XF; Yang, QL ; Chen, GD] U;Univ South China, Ctr Mol Imaging Probe, Hunan Prov Key Lab Tumor Cellular & Mol Pathol, Canc Res Inst,Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Hepatopancreatobiliary Surg, Hengyang 421001, Hunan, Peoples R China.;Hunan Prov Maternal & Child Hlth Care Hosp, Key Lab Birth Defect Res & Prevent, Natl Hlth Commiss, Changsha 410008, Hunan, Peoples R China.;Univ South China, Hengyang Med Sch, MOE Key Lab Rare Pediat Dis, Hengyang 421001, Hunan, Peoples R China.
关键词：
activatable fluorescence imaging;chemodynamic therapy;gas therapy;glutathione depletion;hypothermal photothermal therapy (HPTT);near-infrared II;photoinduced electron transfer
摘要：
Tumor microenvironment (TME)-triggered phototheranostic platform offers a feasible strategy to improve cancer diagnosis accuracy and minimize treatment side effects. Developing a stable and biocompatible molecular phototheranostic platform for TME-activated second near-infrared (NIR-II) fluorescence imaging-guided multimodal cascade therapy is a promising strategy for creating desirable anticancer agents. Herein, a new NIR-II fluorescence imaging-guided activatable molecular phototheranostic platform (IR-FEP-RGD-S-S-S-Fc) is presented for actively targeted tumor imaging and hydrogen sulfide (H2S) gas-enhanced chemodynamic-hypothermal photothermal combined therapy (CDT/HPTT). It is revealed for the first time that the coupling distance between IR-FE and ferrocene is proportional to the photoinduced electron transfer (PET), and the aqueous environment is favorable for PET generation. The part of Cyclic-RGDfK (cRGDfk) peptides can target the tumor and benefit the endocytosis of nanoparticles. The high-concentration glutathione (GSH) in the TME will separate the fluorescence molecule and ferrocene by the GSH-sensitive trisulfide bond, realizing light-up NIR-II fluorescence imaging and a cascade of trimodal synergistic CDT/HPTT/gas therapy (GT). In addition, the accumulation of hydroxyl radicals (center dot OH) and down-regulation of glutathione peroxidase 4 (GPX4) can produce excessive harmful lipid hydroperoxides, ultimately leading to ferroptosis. Trisulfide bond-mediated molecular phototheranostic platform for "activatable" NIR-II imaging-guided enhanced gas-chemo-hypothermal photothermal therapy. A new phototheranostic platform is developed, utilizing cyclic-RGDfk (cRGDfk) peptide for active tumor cell targeting and achieving glutathione (GSH)-rich tumor environment photoinduced electron transfer (PET) blockade for fluorescence-specific illumination and therapy. image
作者机构：
[Li, Yu; Chen, Xiguang; Yin, Liyang; Luo, Gang; Zu, Xuyu; Cao, Renxian; Liu, Zhuoqing; Wu, Ying; Zhou, Xiaolong; Shen, Yingying] Univ South China, Hengyang Med Sch, Canc Res Inst, Affiliated Hosp 1, Hengyang 421001, Peoples R China.;[He, Jun] Univ South China, Hengyang Med Sch, Nanhua Affiliated Hosp, Dept Spine Surg, Hengyang 421001, Peoples R China.
通讯机构：
[Zu, XY; Shen, YY ] U;Univ South China, Hengyang Med Sch, Canc Res Inst, Affiliated Hosp 1, Hengyang 421001, Peoples R China.
关键词：
FUNDC2;GLI1;therapeutic target;triple-negative breast cancer (TNBC)
摘要：
Triple-negative breast cancer (TNBC) lacks effective therapeutic targets and has a poor prognosis, easy recurrence and metastasis. It is urgent and important to explore TNBC treatment targets. Through mass spectrometry combined with qRT-PCR validation in luminal A cells and TNBC cells, high-content screening and clinical sample analysis, FUNDC2 was discovered as a novel target. The function of the outer mitochondrial membrane protein FUNDC2 in breast cancer is still unclear. In this study, we find that FUNDC2 expression in TNBC tissues is significantly higher than that in luminal subtype breast cancer tissues. FUNDC2 silencing in TNBC cells significantly reduces cell proliferation, migration and invasion. As demonstrated in vivo using subcutaneous tumor xenografts in mice, FUNDC2 suppression significantly inhibits tumor growth. The underlying mechanism might be mediated by inactivating its downstream signal AKT/GSK3β and GLI1, a key factor of the Hedgehog signaling pathway. Therefore, FUNDC2 may promote TNBC progression and provide a therapeutic target for treating TNBC.
作者机构：
[Li, Jun; Yan, Wen; Chen, Xiguang; Zu, Xuyu; Luo, Yan; Xiang, Shasha; Wang, Qi; Feng, Jianbo; Zhu, Qingyun] Univ South China, Affiliated Hosp 1, Canc Res Inst, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Zu, XY ; Feng, JB] U;Univ South China, Affiliated Hosp 1, Canc Res Inst, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.
摘要：
Breast cancer (BC) is the most commonly diagnosed cancer and the leading cause of cancer-related death among females. Metastasis accounts for the majority of BC related deaths. One feasible strategy to solve this challenging problem is to disrupt the capabilities required for tumor metastasis. Herein, we verified a novel metastasis suppressive circRNA, circPOKE in BC. circPOKE was downregulated in primary and metastatic BC tissues and overexpression of circPOKE inhibited the metastatic potential but not the proliferative ability of BC cells in vitro and in vivo. Mechanistically, circPOKE competitively binds to USP10, and reduces its binding to Snail, a key transcriptional regulator of EMT, thereby inhibiting Snail stability via the protein-ubiquitination degradation pathway. In addition, we found that circPOKE could be secreted into the extracellular space via exosomes and that exosome-carried circPOKE significantly inhibited the invasive capabilities of BC cells in vitro and in vivo. Furthermore, the levels of circPOKE, USP10 and Snail are clinically relevant in BC, suggesting that circPOKE may be used as a potential therapeutic target for patients with BC metastasis.
作者机构：
[Huang, Yun; Huang, Weiguo; Xiao, Shengjun] Guilin Med Univ, Guangxi Key Lab Tumor Immun & Microenvironm Regula, Guilin 541199, Peoples R China.;[Cheng, Ailan; Huang, Yun; Huang, Weiguo] Univ South China, Canc Res Inst, Hengyang Med Coll, Hengyang 421001, Peoples R China.;[Wei, Dengshuai; Tang, Dongsheng; Wang, Bin] Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, State Key Lab Polymer Phys & Chem, Beijing 100190, Peoples R China.;[Yu, Yingjie] Beijing Univ Chem Technol, State Key Lab Organ Inorgan Composites, Beijing Lab Biomed Mat, Beijing 100029, Peoples R China.
通讯机构：
[Yingjie Yu] S;[Weiguo Huang] G;State Key Laboratory of Organic-Inorganic Composites;Beijing Laboratory of Biomedical Materials;Beijing University of Chemical Technology, Beijing 100029, China<&wdkj&>Guangxi Key Laboratory of Tumor Immunity and Microenvironment Regulation, Guilin Medical University, Guilin 541199, China<&wdkj&>Cancer Research Institute, Hengyang Medical College of University of South China, Hengyang 421001, China
关键词：
DNA damage;Immunogenic cell death;Immunotherapy;Mild hyperthermia;Oxaliplatin
期刊：
CANCER BIOMARKERS,2023年38(2):225-239 ISSN：1574-0153
通讯作者：
Zeng, Zhong;Huang, HF
作者机构：
[Li, Zhitao; Zeng, Zhong; Li, Na; Huang, Hanfei; Wei, Benkai; Fang, Yuan; Jin, Li] Kunming Med Univ, Affiliated Hosp 1, Organ Transplantat Ctr, 295 Xichang Rd, Kunming 650032, Yunnan, Peoples R China.;[Tang, Weiqiang] Univ South China, Hengyang Med Sch, Inst Clin Med, Affiliated Hosp 1, Hengyang, Hunan, Peoples R China.;[Zhao, Dan] Shanghai Jiao Tong Univ, Shanghai Canc Inst, Renji Hosp, Lab Oncogenes & Related Genes,Sch Med, Shanghai, Peoples R China.;[Zhang, Xiaoli] Kunming Med Univ, Gastrointestinal & Hernia Surg, Affiliated Hosp 1, Kunming, Yunnan, Peoples R China.;[Yang, Yang] Baoshan Peoples Hosp, Otorhinolaryngol Head & Neck Surg, Baoshang, Yunnan, Peoples R China.
通讯机构：
[Zeng, Z; Huang, HF ] K;Kunming Med Univ, Affiliated Hosp 1, Organ Transplantat Ctr, 295 Xichang Rd, Kunming 650032, Yunnan, Peoples R China.
关键词：
DNA methylation;HELLS;immune;liver hepatocellular carcinoma;prognosis;tumor-infiltrating
摘要：
BACKGROUND: Lymphoid-specific helicase (HELLS), a SNF2-like chromatin-remodeling enzyme, plays a key role in tumor progression via its DNA methylation function. However, the effects of HELLS on immune infiltration and prognosis in liver hepatocellular carcinoma (LIHC) remain uncertain. METHODS: The Tumor Immune Estimation Resource (TIMER) database was employed to explore the pan-cancer mRNA expression of HELLS and its correlation with immunity. GEPIA2 was used to verify the correlation between HELLS expression and survival. The role of HELLS in cancer was explored via gene set enrichment analysis (Gene Ontology and Kyoto Encyclopedia of Genes and Genomes) and the construction of gene-gene and protein-protein interaction networks (PPI). Additionally, correlations between DNA methylation, HELLS expression, and immune-related genes were explored in LIHC. HELLS expression in LIHC clinical samples was determined using qRT-PCR and western blotting. The effects of downregulated HELLS expression in hepatocellular carcinoma cells was explored via transfection experiments in vitro. RESULTS: High HELLS mRNA expression was identified in several cancers and was significantly associated with poorer prognosis in LIHC. Furthermore, HELLS expression was positively correlated with tumor-infiltrating lymphocytes and immune checkpoint genes in LIHC. Bioinformatics analysis suggested that DNA methylation of HELLS may be associated with the immune response. Results from the TCGA-LIHC dataset, clinical samples, and functional analysis indicated that HELLS contributed to tumor progression in LIHC. CONCLUSION: The study findings demonstrate that HELLS is an important factor in promoting LIHC malignancy and might serve as a potential biomarker for LIHC.
期刊：
Public Health Nursing,2023年40(5):782-789 ISSN：0737-1209
通讯作者：
Zeng, Y
作者机构：
[Huang, Yu-ting; Yue, Qian-qian; Tang, Tian; Zeng, Ying; Hu, Li-feng; Sun, Ying-xue; Zou, Jin-yu] Univ South China, Hengyang Med Sch, Dept Int & Humanist Nursing, Sch Nursing,Hunan Sci Popularizat Educ Base, Hengyang, Peoples R China.;[Zeng, Ying; Zeng, Xi] Univ South China, Canc Res Inst, Hengyang Med Sch, Key Lab Tumor Cellular & Mol Pathol, Hengyang, Peoples R China.;[Zeng, Ying] Univ South China, Hengyang Med Sch, Sch Nursing, Dept Int & Humanist Nursing, Hengyang, Peoples R China.
通讯机构：
[Zeng, Y ] U;Univ South China, Canc Res Inst, Hengyang Med Sch, Key Lab Tumor Cellular & Mol Pathol, Hengyang, Peoples R China.;Univ South China, Hengyang Med Sch, Sch Nursing, Dept Int & Humanist Nursing, Hengyang, Peoples R China.
摘要：
INTRODUCTION: Colorectal cancer (CRC) is associated with a high incidence and mortality rate. Fecal occult blood test (FOBT) is effective in the prevention of CRC. OBJECTIVE: This study aimed to assess knowledge and beliefs regarding FOBT-based screening. METHODS: This study used PubMed, Cochrane Library, MEDLINE Complete, and Web of Science to search for articles. Original full-text studies in English language focusing on knowledge and beliefs of FOBT screening were included. RESULTS: A total of 32 articles were included. This study indicated that the population in most studies had inadequate knowledge and lacked beliefs toward FOBT-based screening. Most of the extracted studies showed that less than half of the participants had heard of FOBT-based screening. Six studies showed that less than 50% of participants had knowledge of FOBT age. Three studies found that less than 40% of participants were aware of the screening interval. Some participants perceived the benefits of FOBT-based screening, while others perceived many barriers to the test. CONCLUSION: Participants' knowledge and belief in FOBT-based screening were insufficient. This review highlights the importance of educational programs to increase knowledge and beliefs regarding FOBT-based screening. It is important to include FOBT-based screening in the health care system to promote the secondary prevention of CRC.
期刊：
AGING AND DISEASE,2023年14(4):1360-1373 ISSN：2152-5250
通讯作者：
Tang, BS;Xu, YM
作者机构：
[Yan, Xinxiang; He, Runcheng; Guo, Jifeng; Chen, Yase; Liu, Zhenhua; Tang, Beisha; Xu, Qian; Jiang, Hong; Sun, Qiying; Zhou, Xun; Shen, Lu] Cent South Univ, Xiangya Hosp, Dept Neurol, Dept Geriatr Neurol, Changsha, Hunan, Peoples R China.;[Yan, Xinxiang; He, Runcheng; Guo, Jifeng; Chen, Yase; Liu, Zhenhua; Tang, Beisha; Xu, Qian; Jiang, Hong; Sun, Qiying; Zhou, Xun; Shen, Lu] Cent South Univ, Xiangya Hosp, Natl Clin Res Ctr Geriatr Disorders, Changsha, Hunan, Peoples R China.;[Xu, Yanming; Huang, Hongyan] Sichuan Univ, West China Hosp, Dept Neurol, Chengdu, Peoples R China.;[Cao, Hongmei] Xi An Jiao Tong Univ, Dept Neurol, Affiliated Hosp 1, Xian, Shaanxi, Peoples R China.;[Wang, Xuejing] Zhengzhou Univ, Dept Neurol, Affiliated Hosp 1, Zhengzhou, Henan, Peoples R China.
通讯机构：
[Tang, BS ] C;[Xu, YM ] S;Cent South Univ, Xiangya Hosp, Changsha, Hunan, Peoples R China.;Sichuan Univ, West China Hosp, Chengdu, Sichuan, Peoples R China.
关键词：
essential tremor;essential tremor plus;the national survey of essential tremor plus in china;registry study;clinical heterogeneity
摘要：
The new term essential tremor (ET) plus was proposed in the 2018 tremor consensus criteria. The National Survey of Essential Tremor Plus in China, a large multicenter registry study, aimed to evaluate the clinical features of pure ET and ET plus and explore possible factors related to ET plus. All patients with ET underwent neurological examination and neuropsychological assessment at 17 clinical sites. The diagnosis was made according to the 2018 consensus criteria. Clinicodemographic characteristics were analyzed. A total of 1160 patients were included, including 546 patients with pure ET and 614 patients with ET plus. The proportion of females was significantly higher in the ET plus than that in the pure ET (P = 0.001). The age at onset (AAO) of pure ET showed a bimodal distribution, with peaks in the 2nd and 5th decades. However, the AAO of the ET plus group demonstrated a skewed distribution, with a single peak in the 6th decade. Female sex (OR=1.645, P<0.001), older age (OR=1.023, P<0.001), lower educational level (OR=0.934, P<0.001), head tremor (OR=1.457, P<0.001), and higher the Tremor Research Group Essential Tremor Rating Assessment Scale (TETRAS)-II scores (OR=1.134, P<0.001) were significantly associated with ET plus. Old age and female sex may contribute to ET plus development. Pure ET showed a bimodal distribution for AAO, whereas ET plus showed a unimodal distribution. It remains unclear whether pure ET and ET plus are merely different stages of a single disease or represent distinct disease entities.
摘要：
Type 2 diabetes mellitus (T2DM) is frequently associated with diabetic cognitive impairment (DCI), and recent studies have shown a strong association between DCI and hippocampal ferroptosis. In this study, we administered dihydromyricetin (DHM) or JNK inhibitor SP600125, to T2DM rats and monitored changes in blood glucose levels, conducted behavioral tests, and detected changes in JNK, inflammatory factors and ferroptosis-related indicators. Our findings demonstrated that T2DM rats displayed signs of cognitive impairment (CI), with ferrozine assays indicating elevated iron content in the hippocampus. Concurrently, there was an increase in p-JNK activity and inflammatory factors IL-6 and TNF-α in the hippocampal region of these rats. Furthermore, we observed elevated levels of Fe(2+), MDA, ROS, LPO, and ACSL4, along with a decrease in GPX4 and GSH, suggesting the occurrence of hippocampal ferroptosis. SP600125 application reversed these changes in the T2DM rats, although it exhibited no significant effects in the control group. Treatment with high and low doses of DHM led to a reduction in p-JNK expression, inflammatory factor-related proteins, and iron accumulation in the hippocampal region, effectively alleviating hippocampal ferroptosis in T2DM rats. No notable effects of DHM were observed in the control group. To conclude, our study suggests that DHM can potentially alleviate hippocampal ferroptosis of T2DM cognitive impairment rats, primarily by suppressing the JNK-inflammatory factor pathway in the hippocampus.
作者机构：
[Zhou, Zhou; Chen, Chen; Zou, JianJun; Zhao, Zheng] Nanjing Med Univ, Nanjing Hosp 1, Dept Clin Pharmacol, Nanjing, Peoples R China.;[Lu, Wei] China Pharmaceut Univ, Sch Basic Med & Clin Pharm, Nanjing, Peoples R China.;[Xiang, Liang; Guo, LeHeng; Shan, YaJie; Zhang, Cheng; Xiang, Lan; Zhao, ZhiHong; Li, XueMei] Hunan Normal Univ, Affiliated Hosp 1, Peoples Hosp Hunan Prov, Dept Neurol, Changsha, Peoples R China.;[Chen, Chen; Zou, JianJun; Zhao, Zheng] China Pharmaceut Univ, Nanjing Hosp 1, Dept Pharm, Nanjing, Peoples R China.;[Wang, BiJun] Univ South China, Affiliated Nanhua Hosp, Clin Res Inst, Hengyang Med Sch, Hengyang, Peoples R China.
通讯机构：
[JianJun Zou; XiaoMing Dai; ZhiHong Zhao] D;Department of Clinical Pharmacology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China<&wdkj&>Department of Pharmacy, Nanjing First Hospital, China Pharmaceutical University, Nanjing, China<&wdkj&>Department of Hepatobiliary Surgery, Hengyang Medical School, The First Affiliated Hospital, University of South China, Hengyang, China<&wdkj&>Department of Neurology, The First Affiliated Hospital (People’s Hospital of Hunan Province), Hunan Normal University, Changsha, China
摘要：
OBJECTIVE: Aneurysmal subarachnoid hemorrhage (aSAH) is an aggressive disease with higher mortality rate in the elderly population. Unfortunately, the previous models for predicting clinical prognosis are still not accurate enough. Therefore, we aimed to construct and validate a visualized nomogram model to predict online the 3-month mortality in elderly aSAH patients undergoing endovascular coiling. METHOD: We conducted a retrospective analysis of 209 elderly aSAH patients at People's Hospital of Hunan Province, China. A nomogram was developed based on multivariate logistic regression and forward stepwise regression analysis, then validated using the bootstrap validation method (n = 1000). In addition, the performance of the nomogram was evaluated by various indicators to prove its clinical value. RESULT: Morbid pupillary reflex, age, and using a breathing machine were independent predictors of 3-month mortality. The AUC of the nomogram was 0.901 (95% CI: 0.853-0.950), and the Hosmer-Lemeshow goodness-of-fit test showed good calibration of the nomogram (p = 0.4328). Besides, the bootstrap validation method internally validated the nomogram with an area under the curve of the receiver operator characteristic (AUROC) of 0.896 (95% CI: 0.846-0.945). Decision curve analysis (DCA) and clinical impact curve (CIC) indicated the nomogram's excellent clinical utility and applicability. CONCLUSION: An easily applied visualized nomogram model named MAC (morbid pupillary reflex-age-breathing machine) based on three accessible factors has been successfully developed. The MAC nomogram is an accurate and complementary tool to support individualized decision-making and emphasizes that patients with higher risk of mortality may require closer monitoring. Furthermore, a web-based online version of the risk calculator would greatly contribute to the spread of the model in this field.
作者机构：
[Zhang, Yang; Yuan, Mei; Sun, Wenjie] Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Dept Neurol, Hengyang, Hunan, Peoples R China.;[Wan, Teng] Shenzhen Univ, Huazhong Univ Sci & Technol, Union Shenzhen Hosp, Dept Neurol,Affiliated Hosp 6,Hlth Sci Ctr, Shenzhen 518060, Peoples R China.;[Guo, Weiming] Shenzhen Univ, Huazhong Univ Sci & Technol, Union Shenzhen Hosp, Dept Sports Med,Affiliated Hosp 6,Hlth Sci Ctr, Shenzhen 518060, Peoples R China.;[Jiang, Weiwei; Xia, Beibei; Wan, Teng; Shen, Xiangru; Fu, Mingyuan] Univ South China, Hengyang Med Coll, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Teng Wan; Mei Yuan] D;Department of Neurology, Huazhong University of Science and Technology Union Shenzhen Hospital, The 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China<&wdkj&>Hengyang Medical College, University of South China, Hengyang, China<&wdkj&>Department of Neurology, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, China
摘要：
Apelin is a natural ligand for the G protein-coupled receptor APJ, and the apelin/APJ system is widely distributed in vivo. Among the apelin family, apelin-13 is the major apelin isoform in the central nervous system and cardiovascular system, and is involved in the regulation of various physiopathological mechanisms such as apoptosis, neuroinflammation, angiogenesis, and oxidative stress. Apelin is currently being extensively studied in the nervous system, and apelin-13 has been shown to be associated with the onset and progression of a variety of neurological disorders, including stroke, neurodegenerative diseases, epilepsy, spinal cord injury (SCI), and psychiatric diseases. This study summarizes the pathophysiological roles of apelin-13 in the development and progression of neurological related diseases.
作者机构：
[Huang, Zhixin; Lu, Haike; Dai, Yingyi; Zhang, Wenli; Huang, Ying] Guangdong Second Prov Gen Hosp, Dept Neurol, 466 Middle Xingang Rd, Guangzhou 510317, Peoples R China.;[Huang, Zhixin] Jinan Univ, Dept Neurol, Fac Med Sci, Guangzhou, Peoples R China.;[Huang, Zhixin] Univ South China, Dept Neurol, Hengyang, Peoples R China.;[Chen, Zhenru] Univ Missouri, Dept Mech & Aerosp Engn, Columbia, MO USA.;[Zou, Fengyuan] Guangzhou AID Cloud Technol, Dept Data Sci, Guangzhou, Peoples R China.
通讯机构：
[Zhixin Huang] D;Department of Neurology, Guangdong Second Provincial General Hospital, Guangzhou, China<&wdkj&>Department of Neurology, Jinan University Faculty of Medical Science, Guangzhou, China<&wdkj&>Department of Neurology, University of South China, Hengyang, China
摘要：
OBJECTIVES: To determine the factors that affect recurrent stroke after acute ischemic stroke, specifically between male and female groups. METHODS: We examined relative factors associated with recurrent stroke in Chinese patients with first-ever ischemic stroke. LASSO (least absolute shrinkage and selection operator) Cox regression were used to determine the predictors of recurrent stroke in the male and female groups. Next, We used Kaplan-Meier survival curves and interactions among these predictors to assess the association between relapse-related factors and recurrent stroke. RESULTS: During one year of follow-up, we documented 42 incidents of recurrent stroke in males and 15 in females. There was no significant difference in the overall recurrence rate between men and women. We finally identified three variables in males and one variable in females associated considerably with recurrent stroke by LASSO Cox regression. In females, good sleep appeared to be the most significant protective factor against recurrent stroke(hazard ratio [HR], 0.21; 95% CI, 0.08-0.57). In the male group, we found two risk factors: atherosclerotic burden (HR, 2.42; 95% CI, 1.30-4.51) and coronary heart disease (HR, 2.98; 95% CI, 1.16-7.66); and one protective factor: domestic/physical activities (HR, 0.45; 95% CI, 0.24-0.83). We also found an interaction between good sleep and domestic/physical activities in males (P(interaction) = 0.016). DISCUSSION: Our data indicate that the factors for recurrent stroke may differ by sex. Engaging in domestic/physical activities may substantially lower recurrent strokes in Chinese adult males. And good sleep in females appears to be more important in preventing stroke recurrence.
摘要：
Glioblastoma multiform (GBM), one of the most common, aggressive primary brain tumours, demonstrates resistance to radiotherapy and chemotherapy after surgical resection and treatment failure. Metformin (MET) has been shown to suppress the proliferative capacity and invasion ability of GBM cells by activating AMPK and inhibiting mTOR, but the effective dose exceeded the maximum tolerated dose. Artesunate (ART) can exert certain anti-tumour effects by activating the AMPK-mTOR axis and inducing autophagy in tumour cells. Therefore, this study investigated the effects of MET combined with ART combination therapy on autophagy and apoptosis in GBM cells. MET combined with ART treatment effectively suppressed the viability, mono-cloning ability, migration and invasion capacities, as well as metastatic ability of GBM cells. The underlying mechanism involved modulation of the ROS-AMPK-mTOR axis, which was confirmed using 3-methyladenine and rapamycin to inhibit or promote the effects of MET combined with ART, respectively. The study findings suggest that MET used in combination with ART can induce autophagy-dependent apoptosis in GBM cells by activating the ROS-AMPK-mTOR pathway, providing a potential new treatment for GBM.
摘要：
In diabetic patients, concomitant cardiovascular disease is the main factor contributing to their morbidity and mortality. Diabetic cardiomyopathy (DCM) is a form of cardiovascular disease associated with diabetes that can result in heart failure. Transforming growth factor-beta (TGF-beta) isoforms play a crucial role in heart remodeling and repair and are elevated and activated in myocardial disorders. Alterations in certain microRNAs (miRNA) are closely related to diabetic cardiomyopathy. One or more miRNA molecules target the majority of TGF-beta pathway components, and TGF-beta directly or via SMADs controls miRNA synthesis. Based on these interactions, this review discusses potential cross-talk between TGF-beta signaling and miRNA in DCM in order to investigate the creation of potential therapeutic targets.
摘要：
Background: Ischemic stroke is the obstruction of cerebral blood flow with a high morbidity. Microglial polarization is a contributing factor for ischemic strokeinduced injury. Here, we focused on function and mechanism of RNA binding protein RPS3 in microglial polarization after ischemic stroke. Methods: Transient middle cerebral artery occlusion (tMCAO) was conducted in SD rats. Infarct area was detected by TTC staining and neurological score was assessed. Fluorescence staining tested neuronal apoptosis and microglial differentiation. Oxygen and glucose deprivation/reoxygenation (OGD/R) was applied for treating microglia. Levels of RPS3, SIRT1, M1 and M2 polarization markers (CD86, iNOS, CD206, Arg-1) were determined by RT-qPCR. Western blot detected RPS3, SIRT1, NLRP3, ASC and Cleaved-caspase-1 expression. RIP assay validated that RPS3 interacted with SIRT1. CCK-8 measured cell viability. Flow cytometry and ELISA assessed M1 and M2 polarization markers. LDH release was detected using colorimetric CytoTox 96 Cytotoxicity kit. Results: RPS3 depletion improved neurological dysfunction and reduced infarction area in rats after tMCAO. Knockdown of RPS3 resulted in increased SIRT1 expression and decreased NLRP3 inflammasome activation, and induced microglia M2 polarization after ischemia-reperfusion (I/R). Besides, RPS3 directly targeted SIRT1 and reduced its expression in microglia. RPS3 silencing suppressed OGD/R-triggered neuronal and microglial cell death through SIRT1. Moreover, RPS3 activated NLRP3 inflammasome and regulated microglial polarization via SIRT1. Conclusion: RPS3 regulates microglial polarization and neuronal injury through SIRT1/NLRP3 pathway, suggesting a novel target for ischemic stroke treatment.Keywords: Ischemic stroke-Microglial polarization-NLRP3 inflammasome-(c) 2023 Elsevier Inc. All rights reserved.
摘要：
BACKGROUND: Alzheimer's disease (AD) is a progressive neurodegenerative disease with high incidence in the elderly population. MicroRNAs have been reported to abnormally expressed in patients with AD. In this study, we investigated the role of inflammation-related miR-511-3p in AD patients and AD cell models. METHOD: The level of miR-511-3p was quantified by Real-Time PCR. The diagnostic value was evaluated by receiver operating characteristic curve (ROC) analysis. The correlation between miR-511-3p expression levels and ini-mental state examination (MMSE) scores, Montreal Cognitive Assessment (MoCA) scores and inflammatory factors was analyzed. The concentrations of IL-1β, IL-6 and TNF-α were measured by Enzyme-Linked Immunosorbent Assay (ELISA) in AD cell model and serum from AD patients. RESULT: Serum miR-511-3p expression was decreased in AD patients and correlated with MMSE score, MoCA score and inflammatory response. MiR-511-3p mimics significantly reversed the effects of Aβ (1)(-)(40) on inflammation in AD cells. ROC curve results showed that miR-511-3p had high diagnostic accuracy in distinguishing normal controls from AD patients. CONCLUSION: Our results show that miR-511-3p is down-regulated in AD patients and has high diagnostic value. MiR-511-3p may participate in the development of AD by regulating the levels of neuroinflammatory factors in AD cells. MiR-511-3p may provide a new perspective for the prevention and pathogenesis of AD.
通讯机构：
[Hu, Hengjing] D;Department of Cardiology, The First Affiliated Hospital of University of South China, Hunan Province, Hengyang, China.;Institute of Cardiovascular Disease and Key Lab for Arteriosclerology of Hunan Province, University of South China, Hengyang, Hunan, China.
期刊：
Journal of Translational Medicine,2023年21(1):1-13 ISSN：1479-5876
通讯作者：
Xiao, ZJ
作者机构：
[He, Bing; Deng, Limin; Zhou, Guijuan; Xu, Yan; Xie, Juan; Chen, Shuangxi; Xiao, Zijian; Wen, Xuanwei; Li, Sijing; Zhu, Guanghua; Xiao, ZJ; Wu, Lin] Univ South China, Affiliated Hosp 1, Multi Res Ctr Brain Disorders, Hengyang Med Sch,Dept Neurol, Hengyang 421001, Hunan, Peoples R China.;[He, Bing; Deng, Limin; Zhou, Guijuan; Xu, Yan; Xie, Juan; Chen, Shuangxi; Xiao, Zijian; Wen, Xuanwei; Li, Sijing; Zhu, Guanghua; Xiao, ZJ; Wu, Lin] Univ South China, Affiliated Hosp 1, Clin Res Ctr Immune Related Encephalopathy Hunan P, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Wu, Lin] Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Dept Neurol, Hengyang 421000, Hunan, Peoples R China.
通讯机构：
[Xiao, ZJ ] U;Univ South China, Affiliated Hosp 1, Multi Res Ctr Brain Disorders, Hengyang Med Sch,Dept Neurol, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Hosp 1, Clin Res Ctr Immune Related Encephalopathy Hunan P, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.
摘要：
Brachial plexus root avulsion (BPRA), a disabling peripheral nerve injury, induces substantial motoneuron death, motor axon degeneration and denervation of biceps muscles, leading to the loss of upper limb motor function. Acetylglutamine (N-acetyl-L-glutamine, NAG) has been proven to exert neuroprotective and anti-inflammatory effects on various disorders of the nervous system. Thus, the present study mainly focused on the influence of NAG on motor and sensory recovery after BPRA in rats and the underlying mechanisms. Male adult Sprague Dawley (SD) rats were subjected to BPRA and reimplantation surgery and subsequently treated with NAG or saline. Behavioral tests were conducted to evaluate motor function recovery and the mechanical pain threshold of the affected forelimb. The morphological appearance of the spinal cord, musculocutaneous nerve, and biceps brachii was assessed by histological staining. Quantitative real-time PCR (qRT‒PCR) was used to measure the mRNA levels of remyelination and regeneration indicators in myocutaneous nerves. The protein levels of inflammatory and pyroptotic indicators in the spinal cord anterior horn were measured using Western blotting. NAG significantly accelerated the recovery of motor function in the injured forelimbs, enhanced motoneuronal survival in the anterior horn of the spinal cord, inhibited the expression of proinflammatory cytokines and pyroptosis pathway factors, facilitated axonal remyelination in the myocutaneous nerve and alleviated atrophy of the biceps brachii. Additionally, NAG attenuated neuropathic pain following BPRA. NAG promotes functional motor recovery and alleviates neuropathic pain by enhancing motoneuronal survival and axonal remyelination and inhibiting the pyroptosis pathway after BPRA in rats, laying the foundation for the use of NAG as a novel treatment for BPRA.
期刊：
FRONTIERS IN NUTRITION,2023年10:1120168 ISSN：2296-861X
通讯作者：
Gui, Q.;Yin, K.
作者机构：
[Zhang, Yaoyuan; Yu, Xinyuan; Gui, Qingjun; Novak, Petr; Zhu, Xiao] Univ South China, Inst Translat Med, Hengyang Med Sch, Hengyang, Hunan, Peoples R China.;[Yu, Xinyuan; Novak, Petr; Zhu, Xiao] Guilin Med Univ, Guangxi Key Lab Diabet Syst Med, Guilin, Peoples R China.;[Gui, Qingjun; Yin, Kai] Southern Med Univ, Dept Gen Practice, Affiliated Hosp 5, Guangzhou, Guangdong, Peoples R China.
通讯机构：
[Gui, Q.] I;[Yin, K.] D;Department of General Practice, Guangdong, China;Institute of Translational Medicine, Hunan, China
关键词：
intestinal barrier;Metabolic Diseases;Natural medicine;gut microbiota remodeling;lifestyle intervention;Bariatric Surgery
摘要：
Physiologically, the intestinal barrier plays a crucial role in homeostasis and nutrient absorption and prevents pathogenic entry, harmful metabolites, and endotoxin absorption. Recent advances have highlighted the association between severely damaged intestinal barriers and diabetes, obesity, fatty liver, and cardiovascular diseases. Evidence indicates that an abated intestinal barrier leads to endotoxemia associated with systemic inflammation, insulin resistance, diabetes, and lipid accumulation, accelerating obesity and fatty liver diseases. Nonetheless, the specific mechanism of intestinal barrier damage and the effective improvement of the intestinal barrier remain to be explored. Here, we discuss the crosstalk between changes in the intestinal barrier and metabolic disease. This paper also highlights how to improve the gut barrier from the perspective of natural medicine, gut microbiota remodeling, lifestyle interventions, and bariatric surgery. Finally, potential challenges and prospects for the regulation of the gut barrier-metabolic disease axis are discussed, which may provide theoretical guidance for the treatment of metabolic diseases.
