摘要：
Evidences indicate that a complex relationship exists among sleep disorders, obesity and insulin resistance. NEU-P11 is a novel melatonin agonist used in treatment of psychophysiological insomnia, and in animal studies NEU-P11 showed sleep-promoting effect. In this study, we applied NEU-P11 on obese rats to assess its potential melatoninergic effects in vivo. Obese models were established using high-fat/high-sucrose-fed for 5 months. NEU-P11 (10 mg/kg)/melatonin (4 mg/kg)/vehicle were administered by a daily intraperitoneal injection respectively for 8 weeks. Our results showed that NEU-P11 or melatonin inhibited both body weight gain and deposit of abdominal fat with no influence on food intake. The impaired insulin sensitivity and antioxidative potency were improved and the levels of plasma glucose, total cholesterol (TC), triglycerides (TG) decreased with an increased in HDL-cholesterol (HDL-c) after NEU-P11 or melatonin administration. These data suggest that NEU-P11, like melatonin, decreased body weight gain and improved insulin sensitivity and metabolic profiles in obese rats. We conclude that NEU-P11 has a melatoninergic effect on regulating body weight in obese rats and also improving metabolic profiles and efficiently enhancing insulin sensitivity. (C) 2009 Elsevier Ltd. All rights reserved.
期刊：
Journal of Nanoscience and Nanotechnology,2010年10(8):4868-4874 ISSN：1533-4880
通讯作者：
Yuan, Yali
作者机构：
[Yuan, Yali; Deng, Jian] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Ding, Jianqiang] Zhenhai Ctr Dis Control & Prevent, Ningbo 315200, Peoples R China.;[Xu, Jinsheng] Hengyang Normal Univ, Dept Chem & Mat Sci, Hengyang 421008, Peoples R China.;[Guo, Jianbo] Univ S China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Yuan, Yali] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
会议主办单位：
(1) School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China; (2) Zhenhai Center for Disease Control and Prevention, Ningbo 315200, China; (3) Department of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, China; (4) School of Public Health, University of South China, Hengyang 421001, China
作者：
Ling, H. -y.;Hu, B.;Hu, X. -b.;Zhong, J.;Feng, S. -d.;Qin, L.;Liu, G.;Wen, G. -b.;Liao, D. -f.*
期刊：
Experimental and Clinical Endocrinology & Diabetes,2012年120(9):553-559 ISSN：0947-7349
通讯作者：
Liao, D. -f.
作者机构：
[Ling, H. -y.; Hu, B.] Univ S China, Sch Med, Dept Physiol, Hengyang, Peoples R China.;[Liao, D. -f.] Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Div Stem Cell Regulat & Applicat, Changsha 410208, Hunan, Peoples R China.;[Ling, H. -y.] Univ S China, Ctr Basic Med Postdoctoral Studies, Hengyang, Peoples R China.;[Hu, X. -b.] Univ S China, Sch Life Sci & Technol, Dept Biochem & Mol Biol, Hengyang, Peoples R China.;[Wen, G. -b.; Zhong, J.] Univ S China, Affiliated Hosp 1, Inst Clin Res, Hengyang, Peoples R China.
通讯机构：
[Liao, D. -f.] Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Div Stem Cell Regulat & Applicat, Changsha 410208, Hunan, Peoples R China.
摘要：
A high performance liquid chromatography method with visible detection (HPLC-VIS) for the determination of malachite green (MG), crystal violet (CV), leucomalachite green (LMG), and leucocrystal violet (LCV) in fish has been developed after clean-up through an immunoaffinity column (IAC). Residues were simultaneously extracted from fish muscle with acetonitrile and ammonium acetate buffer. The leuco-forms, LMG and LCV, were oxidized quantitatively to the chromic CV and MG by reaction with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone. Extracts were then purified on an IAC which prepared by immobilizing the anti-MG-CV antibodies by the sol-gel method. Finally, the eluents were analyzed by HPLC-VIS. The limits of detection were 0.15, 0.1, 0.18 and 0.14 ng/g for MG, CV, LMG and LCV, respectively. The average recoveries in samples fortified with MG, CV, LMG and LCV over the range 0.5-10 ng/g were from 71.6% to 96.8% with RSDs of 5.1-12.3% (n = 6). This novel method was confirmed by liquid chromatography-tandem mass spectrometry with electrospray interface in positive mode using multiple reaction monitoring. (c) 2012 Elsevier B.V. All rights reserved.
摘要：
As a novel kind of nanomaterial with wide potential applications, the adverse effects of carbon nanotubes (CNTs) have recently received significant attention after respiratory exposure. In this study, single-wall carbon nanotubes (SWCNTs) containing different metal contents were intratracheally instilled into lungs of spontaneously hypertensive rats. Pulmonary and cardiovascular system alterations were evaluated at 24 and 72 h post-instillation. Biomarkers of inflammation, oxidative stress and cell damage in the bronchoalveolar lavage fluid (BALF) were increased significantly 24 h post-exposure of SWCNTs. The increased endothelin-1 levels in BALF and plasma and angiotensin I-converting enzyme in plasma suggested endothelial dysfunction in the pulmonary circulation and peripheral vascular thrombosis. These findings suggest that respiratory exposure to SWCNTs can induce acute pulmonary and cardiovascular responses and individuals with existing cardiovascular diseases are very susceptible to SWCNTs exposure. The co-existence of metal residues in SWCNTs can aggravate the adverse effects.
摘要：
With more and more potential applications of carbon nanotubes (CNTs) in different fields, the risk of exposure to CNTs is increasing. The interaction between CNTs and protein in biological media can affect the way cells interact with, recognize and process the nanoparticles, and this has important implications for safety considerations. In this study, the interaction of single-walled and multiwall CNTs with various serum proteins was investigated. The adsorption kinetics of protein to CNTs was investigated and a semi-qualitative analysis was provided by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Matrix assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF MS) was used to identify the protein species binding to CNTs and atomic force microscopy (AFM) was used to vividly demonstrate the adsorption model of protein on CNTs. All the experimental results showed that the adsorption capacity of CNTs for protein was highly dependent on the type, arrangement model, size and surface modification of CNTs. Significant quantity of proteins in serum could be quickly adsorbed by CNTs, mainly including albumin, prealbumin, transferrin, and immunoglobulin. Noncovalent functionalization of CNTs by polyethylene glycol (PEG) could decrease the protein adsorption on CNTs. These results provide crucial insights into human serum proteins binding to different kinds of CNTs, which is important for understanding the safe application of carbon nanotubes.
摘要：
Heme proteins perform various biological functions ranging from electron transfer, oxygen binding and transport, catalysis, to signaling. Although adopting proper native states is very important for these functions, progresses in representative heme proteins, including cytochrome c (cyt c), cytochrome b(5) (cyt b(5)), myoglobin (Mb), neuroglobin (Ngb), cytochrome P450 (CYP) and heme-based sensor proteins such as CO sensor CooA, showed that various native functions, or new functions evolved, are also closely associated with non-native states. The structure and function relationship of heme proteins in non-native states is thus as important as that in native states for elucidating the precise roles of heme proteins in biological systems. (C) 2013 Elsevier Inc. All rights reserved.
作者机构：
[Li, Jie; Huang, Chunlin] Univ S China, Sch Pharm & Life Sci, Dept Biochem & Mol Biol, Hengyang 421001, Peoples R China.;[Guo, Bin; Wang, Xiaoying; Zhu, Weitao; Chen, Bo; Yao, Shouzhuo] Hunan Normal Univ, Minist Educ China, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Hunan, Peoples R China.;[Ouyang, Shan] Shenzhen Entry Exit Inspect & Quarantine Bur Peop, Food Inspect & Quarantine Ctr, Shenzhen 518067, Peoples R China.
通讯机构：
[Guo, Bin] Hunan Normal Univ, Minist Educ China, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Hunan, Peoples R China.
关键词：
Sulfonamides<&wdkj&>Multi-residue<&wdkj&>Dispersive solid-phase extraction<&wdkj&>Animal tissues<&wdkj&>Triple-quadrupole linear ion trap mass spectrometer<&wdkj&>Semi-targeted screening
摘要：
A generic and efficient homolog-targeted approach was used to expand screening and detection of target class of sulfonamides and structural analogs, based on a fast single-tube extraction/partitioning-multifunction adsorption cleanup (SEP/MAC) for class-specific fragmentation-dependent acquisition with a liquid chromatography-hybrid triple-quadrupole linear ion trap mass spectrometer (LC-QqLIT). By combining the two-stage process conducted in a single tube as one-pot protocol, the straightforward SEP/MAC procedure was optimized to offer clean extracts with reasonable recovery (71-109% with RSDs < 20%) and decreased matrix interferences (-9 to 19%) of multiresidual sulfonamide extraction from different tissue samples. The novel use of neutral loss scan of 66 Da (NLS) or precursor ion scanning of m/z 108 (PreS) in positive ion mode was found to achieve more comprehensive coverage of protonated molecular ions of a wide array of sulfonamides including N-4-acetyl and hydroxylamine metabolites plus their possible dimers. Moreover, the PreS-triggered automatically enhanced product ion spectral acquisition enabled simultaneous screening, profiling and confirmation of an unlimited number of analytes belonging to the sulfonamide class within a single analysis. The validation and application results of the generic SEP/MAC-based LC-QqLIT strategy consistently demonstrated favorable performances with acceptable accuracy (67-116%), precision (RSDs < 25%), and sensitivity (LOQs <= 7.5 ng g(-1)) to meet the acceptance criteria for all the sulfonamide-tissue combinations. Thus, the integration of the matrix-independent SEP/MAC procedure and the multiparameter matching algorithm with the unit-resolution LC-QqLIT instrument can serve as a valuable semi-targeted discovery strategy for rapid screening and reliable quantitative/confirmatory analysis of real samples. (C) 2012 Elsevier B.V. All rights reserved.
摘要：
Protein design is a useful method to create novel artificial proteins. A rational approach to design a heterodimeric protein using domain swapping for horse myoglobin (Mb) was developed. As confirmed by X‐ray crystallographic analysis, a heterodimeric Mb with two different active sites was produced efficiently from two surface mutants of Mb, in which the charges of two amino acids involved in the dimer salt bridges were reversed in each mutant individually, with the active site of one mutant modified. This study shows that the method of constructing heterodimeric Mb with domain swapping is useful for designing artificial multiheme proteins.
摘要：
In this study, we report a double-receptor sandwich supramolecule sensing method for the determination of adenosine triphosphate (ATP). One receptor is a uranyl-salophen complex which can bind the triphosphate group in ATP selectively, and another is an anti-adenosine aptamer which is a single-stranded oligonucleotide and can recognize the adenosine group in ATP specifically. The uranyl-salophen complex was immobilized on the surface of amino-silica gel particles and used as the solid phase receptor of ATP. The anti-adenosine aptamer was labeled with a fluorescent group and used as the labeled receptor of ATP. In the procedure of ATP detection, ATP was first combined with the solid phase receptor and then conjugated with the labeled receptor to form a sandwich-type supramolecule. The conditions of fabricating solid phase receptor and the influence of manifold variables on the determination were studied. The experimental results demonstrate that the method has a number of advantages such as high selectivity, high sensitivity, good stability and low cost. Under optimal conditions, the linear range for detection of ATP is 0.2-5.0 nmol/mL with a detection limit of 0.037 nmol/mL The proposed method was successfully applied for the determination of ATP in real samples with the recoveries of 96.8-103.3%. (C) 2012 Elsevier B.V. All rights reserved.
摘要：
A novel small molecule probe, aptamer beacon (AB), was introduced for adenosine (Ade) recognition and quantitative analysis. The Ade aptamer was engineered into an aptamer beacon by adding a gold nanoparticle-modified nucleotide sequence which is complementary to aptamer sequence (FDNA) at the 3'-end of FDNA. The fluorescence signal "turning on" was observed when AB was bound to Ade, which is attributed to a significant conformational change in AB from a FDNA/QDNA duplex to a FDNA-Ade complex. The Ade measurement was carried out in 20 mmol L-1 Tris-HCl buffer solution of pH 7.4, Delta F signal linearly correlated with the concentration of Ade over the range of 2.0 x 10(-8) to 1.8 x 10(-6) mol L-1. The limit of detection (LOD) for Ade is 6.0 x 10(-9) mol L-1 with relative standard deviations (R.S.D) of 3.64-5.36%, and the recoveries were 98.6%, 100%, 102% (n = 6), respectively. The present method has been successfully applied to determine Ade in human urine samples, and the obtained results were in good agreement with those obtained by the HPLC method. Our investigation shows that the unique properties of the AB could provide a promising potential for small molecules detection, and be benefit to extend the application of aptamer beacon technique. (C) 2012 Elsevier B.V. All rights reserved.
期刊：
Journal of Nanoscience and Nanotechnology,2011年11(3):2389-2397 ISSN：1533-4880
通讯作者：
Chen, Chunying
作者机构：
[Liu, Ying; Du, Jiangfeng; Li, Wei; Chen, Chunying; Ge, Cuicui; Li, Yufeng; Qiu, Yang] Inst High Energy Phys, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China.;[Liu, Ying; Du, Jiangfeng; Li, Wei; Chen, Chunying; Ge, Cuicui; Li, Yufeng; Qiu, Yang] Natl Ctr Nanosci & Technol China, Beijing 100190, Peoples R China.;[Gao, Yuxi; Li, Wei; Chai, Zhifang; Chen, Chunying; Li, Bai; Ge, Cuicui; Li, Yufeng] Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Nucl Analyt Tech, Beijing 100049, Peoples R China.;[Du, Jiangfeng] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Chen, Chunying] Inst High Energy Phys, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China.
关键词：
CARBON NANOTUBES<&wdkj&>CATALYST ELEMENTS<&wdkj&>INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY<&wdkj&>NEUTRON ACTIVATION ANALYSIS
摘要：
Commercially available carbon nanotubes (CNT) often contain some quantities of metallic and carbonaceous impurities. These impurities influence their physicochemical properties and performance, and accordingly a number of potential applications. The lack of information of metal impurities may also preclude accurate environmental and health risk assessments for specific CNT materials. To address these needs, a quantitative analysis of the metal contents has been made in a number of commercial carbon nanotubes produced by different manufacturers. More than 20 metals or metalloids were determined by neutron activation analysis. The results indicate arranging from 0.44 to 3 wt% of catalyst residues remained although the producers claim to provide a catalyst-free product. Most of the impurity elements are transition metals, such as Fe, Ni, Mo, Y, Co and Cr. In addition to the expected catalyst residues, other unexpected impurity elements were detected including As, Gd, W, Yb, Sm and so on. Metallic impurities in carbon nanotube materials should come from the large-scale production procedures, post fabrication and post-purification treatments. The analytical results determined by inductively-coupled plasma mass spectrometry show that a further deep purification using conventional acid reflux cannot completely remove the metallic impurities from carbon nanotubes. Post-production clean up is difficult and often incompletely.
