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Construction of artificial peroxidase based on myoglobin scaffold for efficient degradation of meloxicam

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成果类型:
期刊论文
作者:
Weikang Zhang;Yadan Yang;Xiangmin Meng;Fang Wang;Ying-Wu Lin;...
通讯作者:
Xiangmin Meng<&wdkj&>Ying-Wu Lin<&wdkj&>Jiakun Xu
作者机构:
[Yadan Yang; Weikang Zhang; Fang Wang] State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Drugs and Bioproducts, Qingdao Marine Science and Technology Center, Qingdao 266237, China
[Yadan Yang; Weikang Zhang] College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
[Xiangmin Meng] College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: mengxiangmin@qust.edu.cn
[Ying-Wu Lin] School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China. Electronic address: ywlin@usc.edu.cn
[Jiakun Xu] State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Drugs and Bioproducts, Qingdao Marine Science and Technology Center, Qingdao 266237, China. Electronic address: xujk@ysfri.ac.cn
通讯机构:
[Xiangmin Meng] C
[Ying-Wu Lin; Jiakun Xu] S
School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China<&wdkj&>State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Drugs and Bioproducts, Qingdao Marine Science and Technology Center, Qingdao 266237, China<&wdkj&>College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
语种:
英文
关键词:
Biodegradation;Degradation;Environmental remediation;Meloxicam;Myoglobin;Structure-function relationship
期刊:
Journal of Inorganic Biochemistry
ISSN:
0162-0134
年:
2025
卷:
262
页码:
112733
基金类别:
Nagoya University National Natural Science Foundation of China#&#&#32171270
机构署名:
本校为通讯机构
院系归属:
化学化工学院
摘要:
A novel artificial peroxidase has been developed for the efficient degradation of the non-steroidal anti-inflammatory drug meloxicam by combining computer simulation and genetic engineering techniques. The results showed that the artificial peroxidase was able to completely degrade meloxicam within 90 s, with a degradation rate of 100 %, which was much higher than that of natural lacquer (46 %). The reaction time of the artificial enzyme was significantly shorter than that of natural peroxidase (10 min) and laccase (48 h). Further studies showed that the amino acid arrangement of the active si...

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