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Construction of P450 scaffold biocatalysts for the biodegradation of five chloroanilines

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成果类型:
期刊论文
作者:
Yang, Ridong;Shoji, Osami;Lin, Yingwu;Wang, Fang;Che, Hongxia;...
通讯作者:
Xu, JK
作者机构:
[Yang, Ridong; Xu, Jiakun; Wang, Fang] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Lab Marine Drugs & Bioprod,Qingdao Marine Sci & Te, Qingdao 266071, Peoples R China.
[Yang, Ridong; Che, Hongxia] Qingdao Univ Sci & Technol, Coll Biol Engn, Shandong Prov Key Lab Biochem Engn, Qingdao 266042, Shandong, Peoples R China.
[Shoji, Osami] Nagoya Univ, Grad Sch Sci, Dept Chem, Furo Cho,Chikusa Ku, Nagoya 4648602, Japan.
[Lin, Yingwu] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构:
[Xu, JK ] C
Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Lab Marine Drugs & Bioprod,Qingdao Marine Sci & Te, Qingdao 266071, Peoples R China.
语种:
英文
关键词:
Artificial metalloenzyme;Biodegradation;Chloroanilines;H(2)O(2);P450BM3
期刊:
Journal of Hazardous Materials
ISSN:
0304-3894
年:
2025
卷:
488
页码:
137305
基金类别:
National Natural Science Foundation of China [32171263]; Central Public-Interest Scientific Institution Basal Research Fund, CAFS [2023TD71]
机构署名:
本校为其他机构
院系归属:
化学化工学院
摘要:
Chloroanilines represent a class of persistent and highly toxic environmental pollutants, posing significant challenges for green remediation strategies. While P450BM3 monooxygenases are renowned for their ability to catalyze the monooxidation of inert C-H bonds, costly NAD(P)H and complex electron transport systems required for P450BM3 catalysis limit their practical applications. This study pioneers the development of innovative artificial biocatalysts by strategically engineering the active site of P450BM3. Specifically, the substitution of ...

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