作者：
Ling, H. Y.;Ou, H. S.;Lei, X. Y.;Zhu, B. Y.;Yin, W. D.;...
期刊：
RECENT ADVANCES IN CARDIOVASCULAR DISEASE: PROCEEDINGS OF THE 13TH WORLD CONGRESS HEART DISEASE,2007年:263-267
通讯作者：
Liao, D. F.
作者机构：
[Zhu, B. Y.; Yin, W. D.; Lei, X. Y.; Ou, H. S.; Liao, D. F.; Ling, H. Y.] Univ S China, Inst Pharm & Pharmacol, Div Pharmacoprote, Hengyang, Peoples R China.
通讯机构：
[Liao, D. F.] U;Univ S China, Inst Pharm & Pharmacol, Div Pharmacoprote, Hengyang, Peoples R China.
关键词：
MicroRNA;adipocyte;3T3-L1;differentiation;insulin signal
摘要：
This study investigated the MicroRNA expression profile during the differentiation from 3T3-L1 preadipocytes into adipocytes by miRNAs microarray analysis. We found both 3T3-L1 preadipocytes and adipocytes express 331 miRNAs. In adipocyte, 26 miRNAs were upregulated and 2 miRNAs were downregulated compared to 3T3-L1 preadipocytes. Interestingly, we observed that the target genes of miR-21, miR-298, let-7e were associated with insulin signal pathway.
摘要：
To develop a minipig model of type 2 diabetes that simulates the common manifestations of the metabolic abnormalities and resembles the kidney pathology of type 2 diabetes in the human population, male Chinese Bama minipigs were divided into 2 groups (5 in each) and fed with a control diet (CD) or high-fat/ high-sucrose/ high-cholesterol diet (HFSCD) for 5 months. The biochemical parameters of blood and urine, and the oral glucose tolerance test were monitored after the feeding program. The insulin resistance was estimated by the HOMA-IR index and the glucose elimination constant (K(G)), and beta-cell function by the HOMA-beta index and the acute insulin response (AIR). Glomerulosclerosis index (GSI) was semi-quantitated by the degree of glomerular lesions in kidney sections stained with Masson trichrome. Extracellular matrix deposition in the kidney was examined by the protein expression of type IV collagen, connective tissue growth factor (CTGF) and matrix metalloproteinases 2 (MMP-2) using immunohistochemistry. Feeding HFSCD to minipigs markedly caused hyperglycaemia, hyperinsulinaemia and dyslipidaemia. HOMA-IR was significantly increased while HOMA-beta, AIR and K(G) were obviously decreased in the HFSCD group compared with control group. Microalbuminuria, glucosuria and moderate glomerulosclerosis were exhibited in HFSCD-fed minipigs. The expression of type IV collagen and CTGF was elevated whereas that of MMP-2 was reduced in the kidneys of HFSCD group compared with the CD group. We concluded that feeding HFSCD to Chinese Bama minipigs for 5 months can induce humanoid type 2 diabetes and early-stage diabetic nephropathy, and accelerate extracellular matrix deposition and glomerulosclerosis.
作者：
Ling, H. Y.;Ou, H. S.;Lei, X. Y.;Gao, Z. P.;Yin, W. D.;...
期刊：
RECENT ADVANCES IN CARDIOVASCULAR DISEASE: PROCEEDINGS OF THE 13TH WORLD CONGRESS HEART DISEASE,2007年:269-274
通讯作者：
Liao, D. F.
作者机构：
[Gao, Z. P.; Yin, W. D.; Lei, X. Y.; Ou, H. S.; Liao, D. F.; Ling, H. Y.] Univ S China, Inst Pharm & Pharmacol, Div Pharmacoprote, Hengyang, Peoples R China.
通讯机构：
[Liao, D. F.] U;Univ S China, Inst Pharm & Pharmacol, Div Pharmacoprote, Hengyang, Peoples R China.
关键词：
microRNA;adipocyte;3T3-L1;insulin resistance
摘要：
MicroRNAs (miRNAs) are a class of small non-coding RNAs which play an important role in metabolism of glucose and lipid in adipocyte. This study investigated miRNAs expression profiles during the formation process of insulin-resistant adipocyte derived from 3T3-L1 adipocyte. miRNAs were examined by miRNAs microarray analysis..The results showed that 71 miRNAs were upregulated and 33 miRNAs were downregulated during the formation of insulin resistance. Among them, miRNA (miR)-320, -494, -503, -202, -185 and -129-5p were significantly increased. Western blotting analysis showed that Anti-miR-320 increased GLUT4 expression.
摘要：
To investigate the effects of recombinant human adiponectin on the metabolism of diabetic swine induced by feeding a high-fat/high-sucrose diet (HFSD), diabetic animal models were constructed by feeding swine with HFSD for 6 months. The effects of recombinant adiponectin were assessed by detecting the change of plasma glucose levels by commercially available enzymatic method test kits and evaluating the insulin sensitivity by oral glucose tolerance test (OGTT). About 1.5 g purified recombinant adiponectin was produced using a 15-liter fermenter. A single injection of purified recombinant human adiponectin to diabetic swine led to a 2- to 3-fold elevation in circulating adiponectin, which triggered a transient decrease in basal glucose level (P<0.05). This effect on glucose was not associated with an increase in insulin level. Moreover, after adiponectin injection, swine also showed improved insulin sensitivity compared with the control (P<0.05). Adiponectin might have the potential to be a glucose-lowering agent for metabolic disease. Adiponectin as a potent insulin enhancer linking adipose tissue and glucose metabolism could be useful to treat insulin resistance.
摘要：
Inflammation, closely associated with obesity, is emerging as an important risk factor for the pathophysiological development of atherosclerosis and diabetes mellitus. Fat balance is critical in the aetiology of obesity. Lipoprotein lipase is an important enzyme in lipid metabolism. The aim of this study was to investigate the long-term effect of the lipoprotein lipase activator, NO-1886, on inflammation cytokines, adiposity and related diseases in miniature pigs fed a high-fat/high-sucrose/high-cholesterol diet (HFSC diet). Chinese Bama-miniature pigs were fed a control diet or HFSC diet with or without NO-1886 for 5 months. The levels of inflammation-associated cytokines were determined using the antibody arrays. Feeding of the HFSC diet to miniature pigs markedly increased the expression of inflammatory cytokines. On the other hand, supplementation of NO-1886 to HFSC diet decreased the expression of inflammatory cytokines significantly, protecting against the development of atherosclerosis and diabetes mellitus. NO-1886 may have a beneficial effect on the most inflammation-associated cytokines, and this effect may contribute to improving atherosclerosis and diabetes mellitus.
