期刊论文

Yin, Weidong

英文

Cardiovascular Drugs and Therapy

0920-3206

2007

21

1

37-45

10.1007/s10557-007-6002-x

Acknowledgments The authors are indebted to Dr. Li-Xin Li (Oregon University, USA), Dr. He-Sheng Ou (Baylor College of Medicine, USA) and Dr. Zhen-Gao Liu (Ohio State University, USA) for their critical discussions, suggestions, and valuable help in the preparation of the manuscript. This work was supported by the National Natural Science Foundation of China (30470719, 30600249), the National Major Basic Research Program of China (973) (2006CB503808), and the Education Department of Hunan Province (99B11).

本校为第一且通讯机构

Aim: To study the relationship between Daxx expression and the antiapoptotic effects of probucol in THP-1 macrophage. Materials and methods: Apoptosis of THP-1 derived macrophages was induced by exposure to oxidized low density lipoprotein (oxLDL). The development of apoptosis was determined by flow cytometry analysis and nucleic acid-binding dye acridin orange. Reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting and indirect immunofluorescence were used to evaluate the expression of Daxx and caspase-3 at both mRNA and pr...