期刊论文

The Effect of NO - 1886 on the Expression of Protein Kinase C in the Kidneys of Diabetic Minipig Model Induced by High - sucrose and High - fat Diet

中文

医学研究杂志

1673-548X

2008

37

11

59-63

10.3969/j.issn.1673-548X.2008.11.020

ws-c2005034:湖南省卫生计生委科研项目 04TH4001,05TH4002,06TH400:湖南省科技厅科研项目 05JJ20006,04JJ3058:湖南省自然科学基金

本校为第一机构

目的 探讨合成化合物脂蛋白酯酶活化剂NO-1886对高糖高脂喂养小型猪肾脏蛋白激酶C表达的影响.方法 广西巴马小型猪共15只,按体重随机分为3组,5只/组:正常对照组,喂正常猪饲料;高糖高脂组,喂高糖高脂饲料;加药组,喂加入1.0%NO-1886的高糖高脂饲料.动物分栏喂养,每日投食3次,日粮为体重的4%,自由饮水.实验期为5个月.实验前和实验过程中每月末分别抽取血样,葡萄糖氧化酶法检测空腹血糖;GPO-PAP酶法测定血浆三酰甘油;放射免疫法测血浆胰岛素.第5个月从左肾下极的相同部位取部分组织(包括皮质和髓质),HE染色以观察细胞显微结构,同时免疫组化染色;免疫组织化学方法和Western blot方法分别检测肾脏蛋白...

Objective To investigate the protective the effect of NO - 1886 on the expression of protein kinase C in the kidneys of diabetic minipig model induced by high - sucrose and high - fat diet. Methods 15 Guangzhou minipigs aged 3 months were randomly divided into 3 groups of normal control,diabetes, diabbetes treatment, which were fed by basaldiet, high sucrose and high fat feed or with 1. 0% No - 1886 respectively. These minipigs were killed at the end of 5th month. Minipigs fed with high fat/high sucrose diet were treated with No- 1886,and The f...