期刊论文

中文

动物学杂志

0250-3263

2006

41

6

41-48

10.3969/j.issn.0250-3263.2006.06.007

国家自然科学基金项目（No.30370675）； 湖南省自然科学基金项目（No.05JJ20006,04JJ3058）； 湖南省科技计划项目（No.04TH4001,05TH4002）；

本校为第一机构

Oo为研究高脂高糖高胆固醇(HFSCD)诱导的2型糖尿病小型猪肾脏细胞外基质(ECM)的表达情况，将10头广西巴马小型猪分别喂正常和HFSCD饲料，收集血、尿液，测定其生化指标，进行口服葡萄糖耐量试验。5个月后取部分肾组织，行H．E和Masson染色，免疫组织化学方法检测ECM相关蛋白的表达。结果表明，喂养HFSCD后，小型猪血糖、血脂和胰岛素明显升高，出现胰岛素抵抗和β细胞功能紊乱；尿糖和微量白蛋白升高；肾脏纤维连接蛋白(FN)和Ⅳ型胶原的表达明显增加，而Ⅰ型胶原无表达。这说明HFSCD喂养广西巴马小型猪5个月能成功建立早期糖尿病肾病模型，促进FN和Ⅳ型胶原的表达。

To investigate the differential expressions of extraeellular matrix in the kidneys of type 2 diabetic mini-pigs induced by high-fat/high-sucrose/high-cholesterol diet （HFSCD）, 10 male Guangxi Bama mini-pigs were fed normal control diet or HFSCD （5 for each group）, and biochemical parameters in blood and urine and oral glucose tolerance test were monitored after the feeding programme. Five months later, each left kidney was removed, routinely stained with HE and Masson＇ s trichrome staining. Extracellular matrix expression in the kidneys wa...