A novel sensor based on multi-walled carbon nanotubes and boron-doped double-layer molecularly imprinted membrane for the analysis of SCZ in pharmaceutical and biological samples
作者：
Peng, Pengcheng;Liao, Lifu;Yu, Zhaohui;Jiang, Min;Deng, Jian;...
期刊：
International Journal of Environmental Analytical Chemistry ,2019年99(15):1495-1514 ISSN：0306-7319
通讯作者：
Xiao, Xilin
作者机构：
[Peng, Pengcheng; Liao, Lifu; Jiang, Min; Deng, Jian] Univ South China, Sch Chem & Chem Engn, Key Discipline Lab Natl Def Biotechnol Uranium Mi, Hengyang City, Peoples R China;[Yu, Zhaohui; Xiao, Xilin] Univ South China, Sch Resource & Environm & Safety Engn, Hengyang City, Peoples R China
通讯机构：
[Xiao, Xilin] U;Univ South China, Sch Resource & Environm & Safety Engn, Hengyang City, Peoples R China.
关键词：
Secnidazole;multi-wall carbon nanotubes;electrochemical sensor;differential pulse voltammetry;Molecularly imprinted polymer
摘要：
A molecularly imprinted electrochemical sensor for the rapid detection of the anti-parasitic drug Secnidazole (SCZ) is reported. In this work, the build electrochemical sensor was based on a carbon paste electrode (CPE) modified with multi-wall carbon nanotubes (MWCNTs) and boron-embedded duplex molecularly imprinted composite membranes (B-DMICMs), that significantly increased the efficiency of the sensor for the detection of template molecule SCZ. Density functional theory (DFT) was employed to study the interactions between the template and monomers to select appropriate functional monomers for rational design of the B-DMICMs.The optimal experimental conditions were optimised for the factors affecting the performance of the sensor. Under the optimal conditions, the reduction peak currents of SCZ by differential pulse voltammetry increased linearly with SCZ concentration in the range from 3.0 x 10(-4) to 1.0 x 1.0(-6) mol L-1 and 1.0 x 1.0(-6) to 1.91 x 10(-8) mol L-1 with a detection limit of 1.72 x 10(-8) mol L-1 for secnidazole, which is significantly lower than those in the currently used methods and in previous reports. This method offers low cost, sensitive and effective determination of SCZ and can potentially be used for detection of SCZ in pharmaceutical and biological samples with good precision and accuracy.
语种：
英文
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Preparation and application of a carbon paste electrode modified with multi-walled carbon nanotubes and boron-embedded molecularly imprinted composite membranes
作者：
Wang, Hongjuan;Qian, Duo;Xiao, Xilin* ;Deng, Chunyan;Liao, Lifu;...
期刊：
Bioelectrochemistry ,2018年121:115-124 ISSN：1567-5394
通讯作者：
Xiao, Xilin;Deng, Jian
作者机构：
[Xiao, XL; Deng, Jian; Wang, Hongjuan; Xiao, Xilin; Qian, Duo; Liao, Lifu; Lin, Ying-Wu] Univ South China, Sch Chem & Chem Engn, Key Discipline Lab Natl Def Biotechnol Uranium Mi, Hengyang 421001, Hunan, Peoples R China.;[Deng, Chunyan] Cent S Univ, Coll Chem & Chem Engn, Key Lab Resources Chem Nonferrous Met, Changsha 410083, Hunan, Peoples R China.
通讯机构：
[Xiao, XL; Deng, J] U;Univ South China, Sch Chem & Chem Engn, Key Discipline Lab Natl Def Biotechnol Uranium Mi, Hengyang 421001, Hunan, Peoples R China.
关键词：
Tinidazole;Density functional theory;Multi-walled carbon nanotubes;Boron-embedded molecular imprinting composite membrane;Electrochemical sensor
摘要：
An innovative electrochemical sensor was fabricated for the sensitive and selective determination of tinidazole (TNZ), based on a carbon paste electrode (CPE) modified with multi-walled carbon nanotubes (MWCNTs) and boron-embedded molecularly imprinted composite membranes (B-MICMs). Density functional theory (DFT) calculations were carried out to investigate the utility of template-monomer interactions to screen appropriate monomers for the rational design of B-MICMs. The distinct synergic effect of MWCNTs and B-MICMs was evidenced by the positive shift of the reduction peak potential of TNZ at B-MICMs/MWCNTs modified CPE (B-MICMs/MWCNTs/CPE) by about 200 mV, and the 12-fold amplification of the peak current, compared with a bare carbon paste electrode (CPE). Moreover, the coordinate interactions between trisubstituted boron atoms embedded in B-MICMs matrix and nitrogen atoms of TNZ endow the sensor with advanced affinity and specific directionality. Thereafter, a highly sensitive electrochemical analytical method for TNZ was established by different pulse voltammetry (DPV) at B-MICMs/MWCNTs/CPE with a lower detection limit (1.25 × 10−12 mol L−1) (S/N = 3). The practical application of the sensor was demonstrated by determining TNZ in pharmaceutical and biological samples with good precision (RSD 1.36% to 3.85%) and acceptable recoveries (82.40%–104.0%). © 2017 Elsevier B.V.
语种：
英文
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基于复式分子印迹膜识别的左奥硝唑传感器
作者：
钱多;王红娟;何军;邓健
期刊：
分析试验室 ,2017年36(4):466-470 ISSN：1000-0720
作者机构：
[钱多; 王红娟; 何军; 邓健] 南华大学化学化工学院, 衡阳, 421001
关键词：
奥硝唑;复式分子印迹膜;电化学传感器
摘要：
建立了基于导电聚合物-硅氧缩聚物复式分子印迹膜的左奥硝唑(S-ONZ)对映体检测方法。以3 -(苯氨基)丙基三甲氧基硅烷和2 -氨基苯硼酸为电聚合单体,在碳糊电极表面电沉积连有Si(OCH_3)_3和B(OH)_2基团的聚苯胺型导电膜;以3 -氨丙基三甲氧基硅烷为功能单体、四乙氧基硅烷为交联剂,模板分子S-ONZ存在的条件下经溶胶-凝胶法在导电膜表面共价连接形成硅氧缩聚物分子印迹膜。B(Ⅲ)的引入使印迹位点嵌入路易斯酸中心,由此形成的配位相互作用使印迹位点对模板分子具有更高的对映识别性和更强的结合能力。最优实验条件下,在6.0×10~(-8)~3.5 ×10~(-4) mol/L浓度范围内S-ONZ的还原峰电流与其浓度呈良好的线性关系,检测限为11 nmol/L。传感器已用于检测片剂、血清及尿液中S-ONZ含量,回收率为95.1% ~106%,RSD为3.3% ~4.2%。
语种：
中文
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A highly sensitive and selective sensor based on a graphene-coated carbon paste electrode modified with a computationally designed boron-embedded duplex molecularly imprinted hybrid membrane for the sensing of lamotrigine
作者：
Wang, Hongjuan;Qian, Duo;Xiao, Xilin* ;Gao, Shuqin;Cheng, Jianlin;...
期刊：
Biosensors and Bioelectronics ,2017年94:663-670 ISSN：0956-5663
通讯作者：
Xiao, Xilin;Deng, Jian
作者机构：
[Xiao, XL; Deng, Jian; Wang, Hongjuan; Xiao, Xilin; Qian, Duo; Liao, Lifu; He, Bo] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Gao, Shuqin] Univ South China, Coll Med, Hengyang 421001, Hunan, Peoples R China.;[Cheng, Jianlin] Univ South China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Xiao, XL; Deng, J] U;Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Computational design;Duplex molecularly imprinted hybrid membrane;Electrochemical sensor;Lamotrigine
摘要：
An innovative electrochemical sensor, based on a carbon paste electrode (CPE) modified with graphene (GR) and a boron-embedded duplex molecularly imprinted hybrid membrane (B-DMIHM), was fabricated for the highly sensitive and selective determination of lamotrigine (LMT). Density functional theory (DFT) was employed to study the interactions between the template and monomers to screen appropriate functional monomers for rational design of the B-DMIHM. The distinct synergic effect of GR and B-DMIHM was evidenced by the positive shift of the reduction peak potential of LMT at B-DMIHM/GR modified CPE (B-DMIHM/GR/CPE) by about 300 mV, and the 13-fold amplification of the peak current, compared to a bare carbon paste electrode (CPE). The electrochemical reduction mechanism of lamotrigine was investigated by different voltammetric techniques. It was illustrated that square wave voltammetry (SWV) was more sensitive than different pulse voltammetry (DPV) for the quantitative analysis of LMT. Thereafter, a highly sensitive electroanalytical method for LMT was established by SWV at B-DMIHM/GR/CPE with a good linear relationship from 5.0×10<sup>−8</sup>to 5.0×10<sup>−5</sup>and 5.0×10<sup>−5</sup>to 3.0×10<sup>−4</sup> mol L<sup>−1</sup>with a lower detection limit (1.52×10<sup>−9</sup> mol L<sup>−1</sup>) based on the lower linear range(S/N=3). The practical application of the sensor was demonstrated by determining the concentration of LMT in pharmaceutical and biological samples with good precision (RSD 1.04–4.41%) and acceptable recoveries (92.40–107.0%). ©2017 Elsevier B.V.
语种：
英文
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Enantioselective determination of S-ornidazole by using carbon paste electrode modified with boron-embedded conductive copolymer-polysiloxane-based molecularly imprinted hybrid film
作者：
Wang, Hongjuan;Qian, Duo;Xiao, Xilin* ;He, Bo;Gao, Shuqin;...
期刊：
Electrochimica Acta ,2017年246:338-347 ISSN：0013-4686
通讯作者：
Xiao, Xilin;Deng, Jian
作者机构：
[Xiao, XL; Deng, Jian; Wang, Hongjuan; Xiao, Xilin; Qian, Duo; Liao, Lifu; He, Bo] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Gao, Shuqin] Univ South China, Sch Med, Hengyang 421001, Hunan, Peoples R China.;[Shi, Han] Bur Anim Husb & Fisheries, Shaodong 422800, Hunan, Peoples R China.
通讯机构：
[Xiao, XL; Deng, J] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
S-ornidazole;Enantioselective determination;Molecularly imprinted hybrid film;Electrochemical sensor
摘要：
Highly selective determination of enantiomers by electrochemical sensors with simple recognition elements is of great interest in pharmaceutics, biochemistry and medical fields. The present work developed an innovative electrochemical sensor, which based on boron-embedded molecularly imprinted hybrid film (B-MIHF) modified carbon paste electrode (CPE), for enantioselective determination of S-ornidazole (S-ONZ). A boron-embedded conductive copolymer was firstly electro polymerized on carbon paste electrode (CPE), and then molecularly imprinted polysiloxanes (MIP) were hydrolyzed and condensed over the conductive copolymer layer via a sol-gel process. The as-prepared B-MIHF, comprised the advantages of boron-embedded conductive copolymer and molecularly imprinted polysiloxanes, could not only implement direct signal transformation between imprinted cavities and sensing elements, but show amazing selectivity to template S-ornidazole. Above all, the introduction of coordinate interaction formed between trisubstituted boron atoms embedded in B-MIHF matrix and nitrogen atoms of template endow the sensor with intensive enantioselectivity. Under optimum experiments conditions, the reduction peak currents of S-ONZ by differential pulse voltammetry were linear to S-ONZ concentrations in the range from 6.0 x 10(8) to 1.0 x 10(6) mol L-1 and 1.0 x 10(-6) to 3.5 x 10(4) mol L-1 with a detection limit of 1.1 x 10(-8) mol L-1. The B-MIHF-based sensor has been successfully applied for the determination of S-ONZ in pharmaceutical and biological samples with good precision (RSD 2.25-4.11%) and acceptable recoveries (96.00-108.00%). (C) 2017 Elsevier Ltd. All rights reserved.
语种：
英文
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Computational design and fabrication of core-shell magnetic molecularly imprinted polymer for dispersive micro-solid-phase extraction coupled with high-performance liquid chromatography for the determination of rhodamine 6G
作者：
Xie, Jin;Xie, Jie;Deng, Jian* ;Fang, Xiangfang;Zhao, Haiqing;...
期刊：
JOURNAL OF SEPARATION SCIENCE ,2016年39(12):2422-2430 ISSN：1615-9306
通讯作者：
Deng, Jian
作者机构：
[Zhao, Haiqing; Wang, Hongjuan; Deng, Jian; Qian, Duo; Xie, Jin; Fang, Xiangfang] Univ South China, Sch Chem & Chem Engn, Hengyang, Hunan, Peoples R China.;[Xie, Jie] China Agr Univ, Coll Vet Med, Beijing, Peoples R China.;[Xie, Jie] China Agr Univ, State Key Lab Agrobiotechnol, Beijing, Peoples R China.
通讯机构：
[Deng, Jian] U;Univ South China, Sch Chem & Chem Engn, Hengyang, Hunan, Peoples R China.
关键词：
Dispersive micro-solid-phase extraction;High-performance liquid chromatography;Magnetic surface molecularly imprinted polymers;Rhodamine 6G
摘要：
A novel core–shell magnetic nano-adsorbent with surface molecularly imprinted polymer coating was fabricated and then applied to dispersive micro-solid-phase extraction followed by determination of rhodamine 6G using high-performance liquid chromatography. The molecularly imprinted polymer coating was prepared by copolymerization of dopamine and m-aminophenylboronic acid (functional monomers), in the presence of rhodamine 6G (template). The selection of the suitable functional monomers was based on the interaction between different monomers and the template using the density functional theory. The ratios of the monomers to template were further optimized by an OA<inf>9</inf>(3<sup>4</sup>) orthogonal array design. The binding performances of the adsorbent were evaluated by static, kinetic, and selective adsorption experiments. The results reveal that the adsorbent possesses remarkable affinity and binding specificity for rhodamine 6G because of the enhanced Lewis acid-base interaction between the B(Ш) embedded in the imprinted cavities and the template. The nano-adsorbent was successfully applied to dispersive micro-solid-phase extraction coupled to high-performance liquid chromatography for the trace determination of rhodamine 6G in samples with a detection limit of 2.7 nmol/L. Spiked recoveries ranged from 93.0–99.1, 89.5–92.7, and 86.9–105% in river water, matrimony vine and paprika samples, respectively, with relative standard deviations of less than 4.3%. ©2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
语种：
英文
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铝掺杂表面分子印迹聚合物修饰电极检测食品中罗硝唑残留
作者：
赵海青;江梦银;冯莹莹;吴安乐;詹晓珠;...
期刊：
分析测试学报 ,2016年35(8):993-998 ISSN：1004-4957
作者机构：
南华大学化学化工学院, 湖南, 衡阳, 421001;福建省三明市农产品质量安全检验中心, 福建, 三明, 365000;[赵海青; 江梦银; 冯莹莹; 吴安乐; 邓健] 南华大学化学化工学院, 湖南, 衡阳, 421001;[詹晓珠] 福建省三明市农产品质量安全检验中心, 福建, 三明, 365000
关键词：
罗硝唑;磁性表面分子印迹聚合物;差分脉冲溶出伏安法;铝掺杂
摘要：
以罗硝唑(Ronidazole,RNZ)为模板,3-氨丙基三乙氧基硅烷为功能单体,Al(Ⅲ)为路易斯酸掺杂剂,用溶胶-凝胶法制备核壳型磁性分子印迹聚合物,借助磁力作用将其修饰于磁性玻碳电极表面,制得RNZ电化学印迹传感器。以透射电镜、红外光谱、X-射线衍射及电化学方法等对该传感器进行表征,并对影响传感器性能的主要参数进行优化。结果表明,相比基于非掺杂印迹聚合物和非分子印迹聚合物的传感器,铝掺杂印迹传感器对罗硝唑具有更强的结合能力和更高的识别选择性。用差分脉冲溶出伏安法进行定量测定时,RNZ的还原峰电流与其浓度在0.05 ~ 50.0 μmol/L范围内呈良好线性关系(r = 0.997 3),检出限为0.015 μmol/L。对牛奶、鸡蛋中RNZ的回收率为88.6%~ 97.0%,相对标准偏差(RSD)为2.9%~ 4.6%。
语种：
中文
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核壳型磁性表面分子印迹聚合物的制备及对罗丹明6G吸附性能研究
作者：
谢进;赵海青;邓健
期刊：
精细化工中间体 ,2016年46(1):80-84 ISSN：1009-9212
作者机构：
南华大学化学化工学院,湖南衡阳,421001;[邓健; 谢进; 赵海青] 南华大学
关键词：
罗丹明6G;磁性表面分子印迹聚合物;固相萃取;分光光度法
摘要：
以罗丹明6G为模板分子,多巴胺和3-氨基苯硼酸为功能单体和交联剂,采用表面分子印迹技术,首次制备了对罗丹明6G具有优良吸附作用的核壳型磁性表面分子印迹聚合物。应用扫描电镜、透射电镜、红外光谱等对其进行表征,通过静态、动态和选择性吸附实验,研究了该分子印迹聚合物的识别选择性和吸附容量,并建立了固相萃取-分光光度法测定实际样品中痕量罗丹明6G的新方法,检测限为2.7nmol/L,回收率为86.9%-105.0%。
语种：
中文
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Carbon paste electrode modified with duplex molecularly imprinted polymer hybrid film for metronidazole detection
作者：
Xiao, Ni;Deng, Jian* ;Cheng, Jianlin;Ju, Saiqin;Zhao, Haiqing;...
期刊：
Biosensors and Bioelectronics ,2016年81:54-60 ISSN：0956-5663
通讯作者：
Deng, Jian
作者机构：
[Xiao, Ni; Zhao, Haiqing; He, Jun; Deng, Jian; Qian, Duo; Ju, Saiqin; Xie, Jin] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Cheng, Jianlin] Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Deng, Jian] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Anilinomethyltriethoxysilane;Duplex molecularly imprinted polymer;Electrochemical sensor;Electropolymerization;Metronidazole
摘要：
A novel electrochemical sensor based on duplex molecularly imprinted polymer (DMIP) hybrid film modified carbon paste electrode (CPE) has been developed for highly sensitive and selective determination of metronidazole (MNZ). A conductive poly(anilinomethyltriethoxysilane) film is firstly electrodeposited on the surface of a CPE, and then a molecularly imprinted polysiloxane (MIPS) membrane is covalently covered on the film via sol-gel process. The as-constructed DMIP hybrid film, combining the advantages of MIPS and conducting MIP, can make feasible the direct and efficient signal transformation between the target analyte and the transducer, as well as enhance the imprinting recognition capability, mass transfer efficiency and the detection sensitivity. Under optimized conditions, the reduction peak currents of MNZ are linear to MNZ concentrations in the range from 4.0×10-7 to 2.0×10-4 mol L-1 with a detection limit of 9.1×10-8 mol L-1. The RSD values vary from 2.9% to 4.7% for intra-day and from 3.4% to 4.2% for inter-day precision. The DMIP-based sensor has been successfully applied for the determination of MNZ in biological and pharmaceutical samples. The accuracy and reliability of the method is further confirmed by high performance liquid chromatography. © 2016 Elsevier B.V.
语种：
英文
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基于自主创新的研究生化学实验教学改革与实践
作者：
肖锡林;林英武;王宏青;刘慧君;赵玉宝;...
期刊：
实验科学与技术 ,2016年14(6):87-92 ISSN：1672-4550
作者机构：
南华大学 化学化工学院,湖南 衡阳,421001
关键词：
研究生;化学实验;自主创新;教学改革;培养质量
摘要：
为了培养学生的创新精神,激发学生创新意识,使学生成为高素质的创新人才,该文通过比较实验考核成绩和学生科学研究成果,调查研究生对自主创新实验教学新方法的看法和接受程度来评价新教学方法的优劣,探讨了研究生化学实验教学在研究生培养过程中的作用以及化学实验教学对人才培养的多方面功能,初步研究和设计了适合地方高校研究生培养的化学实验教学方法。实践表明,通过系列化学实验教学的改革,提高了研究生的培养质量,增强了研究生的自主创新能力,具有广泛推广价值。
语种：
中文
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制药工程专业英语教学改革小议
作者：
孙允凯;吉文婷;邓健
期刊：
广东化工 ,2015年42(15):250,231 ISSN：1007-1865
作者机构：
南华大学化学化工学院,湖南衡阳,421001;南华大学经济管理学院,湖南衡阳,421001
关键词：
制药工程;专业英语;教学改革
摘要：
制药工程专业英语教学宗旨在于培养学生在该专业领域内具有扎实的英语读说能力,是培养高素质制药工程科技工作者不可或缺的一部分。针对该专业英语教学目标、教学内容、师资队伍和考核形式等教学方面的不足,提出了合理化建议,以期提高制药工程专业英语教学水平。
语种：
中文
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Determination of dimetridazole using carbon paste electrode modified with aluminum doped surface molecularly imprinted siloxane
作者：
Hu, Canhui;Deng, Jian* ;Xiao, Xilin;Zhan, Xiaozhu;Huang, Kaihui;...
期刊：
Electrochimica Acta ,2015年158:298-305 ISSN：0013-4686
通讯作者：
Deng, Jian
作者机构：
[Huang, Kaihui; Xiao, Ni; Hu, Canhui; Xiao, Xilin; Deng, Jian; Ju, Saiqin] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Zhan, Xiaozhu] Sanming Supervis & Testing Ctr Qual & Safety Agro, Fujian 365000, Peoples R China.
通讯机构：
[Deng, Jian] U;Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
dimetridazole;aluminum doping;surface molecularly imprinted siloxane;carbon paste electrode;differential pulse stripping voltammetry
摘要：
For the first time, a simple and highly sensitive electrochemical dimetridazole (DMZ) sensor based on a carbon paste electrode modified with core-shell aluminum doped surface molecularly imprinted siloxane has been developed and tested. Because of aluminum doping, the surface imprinting sensor exhibits higher rebinding capacity, recognition ability and affinity for DMZ in comparison with the aluminum free and the non-imprinted ones. In addition, the as-prepared sensor shows good stability and satisfactory reproducibility for the determination of DMZ. Under optimal experimental conditions, the peak currents by differential pulse stripping voltammetry were found to vary linearly with DMZ concentrations in the range from 1.0 × 10−8 to 1.0 × 10−6 mol L−1 and 1.0 × 10−6 to 1.0 × 10−4 mol L−1, with a detection limit of 3.6 × 10−9 mol L−1. This sensor was successfully employed to detect DMZ in egg, milk powder, and pig feed samples with acceptable recoveries of 91.4% to 107.6%, which was verified by high performance liquid chromatography.
语种：
英文
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Highly sensitive and selective determination of melamine in milk using glassy carbon electrode modified with molecularly imprinted copolymer
作者：
Deng, Jian* ;Ju, Saiqin;Liu, Yating;Xiao, Ni;Xie, Jin;...
期刊：
Food Analytical Methods ,2015年8(10):2437-2446 ISSN：1936-9751
通讯作者：
Deng, Jian
作者机构：
[Xiao, Ni; Zhao, Haiqing; Deng, Jian; Ju, Saiqin; Xie, Jin] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Liu, Yating] Jiangxi Jingde PVMTech Co Ltd, Jingdezhen 333000, Jiangxi, Peoples R China.
通讯机构：
[Deng, Jian] U;Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Electropolymerization;Gallic acid;Melamine;Molecularly imprinted polymer;o-Phenylenediamine;Square wave voltammetry
摘要：
A novel electrochemical sensor was constructed for highly sensitive and selective determination of melamine by depositing a molecularly imprinted copolymer film, prepared by electrocopolymerization of gallic acid and o-phenylenediamine from aqueous electrolyte solution, onto glassy carbon electrode surface. The morphologic feature of the modified electrode was characterized via cyclic voltammetry, electrochemical impedance spectrometry, and scanning electron microscopy. The interactions between the molecularly imprinted copolymer and melamine cause the decrease in the peak currents of hexacyanoferrate, which could be used for electrochemical sensing of melamine. In this study, square wave voltammetry was selected as transducing mechanism. Several important parameters controlling the performance of the molecularly imprinted polymer (MIP) electrode were investigated and optimized. In the optimal conditions, the sensor response was sensitive to melamine from 5.0 to 100.0 nmol L<sup>−1</sup>with good selectivity. The limit of detection based on a signal to noise ratio of 3 was low up to 1.4 nmol L<sup>−1</sup>. The method was applied successfully to determine melamine in milk products with satisfactory precision (2.11~3.38 % of relative standard deviation (RSD)) and acceptable recoveries (96.6~106.4 %), demonstrating a promising feature for applying the MIP sensor to trace detection of melamine in milk and other related products. [Figure not available: see fulltext.] ©2015, Springer Science+Business Media New York.
语种：
英文
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医学基础化学网络教程设计与探索
作者：
肖锡林;廖力夫;邓健;林英武;袁亚莉;...
期刊：
中国现代教育装备 ,2015年(17):64-66 ISSN：1672-1438
作者机构：
南华大学化学化工学院湖南衡阳 421001
关键词：
医学基础化学;网络教程;多媒体教学
摘要：
医学基础化学是将化学学科中与医学关系密切的内容整合而成的，是医学专业学生的一门重要基础课程。医学基础化学课程教学内容多，概念抽象，学生难以理解。应用网络新手段，设计医学基础化学网络教程，科学安排学习模块，大量使用flash动画演示、文本、图形、音频及视频等多媒体素材，引导学生进行探索和发现式学习，极大地激发了学生学习兴趣，取得了很好的教学效果，提高了教学质量，推动了教学改革发展。
语种：
中文
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Determination of leucomalachite green, leucocrystal violet and their chromic forms using excitation–emission matrix fluorescence coupled with second-order calibration after dispersive liquid–liquid microextraction
作者：
Ju, Saiqin;Deng, Jian* ;Cheng, Jianlin;Xiao, Ni;Huang, Kaihui;...
期刊：
Food Chemistry ,2015年185:479-487 ISSN：0308-8146
通讯作者：
Deng, Jian
作者机构：
[Xiao, Ni; Huang, Kaihui; Hu, Canhui; Zhao, Haiqing; Deng, Jian; Ju, Saiqin; Xie, Jin] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Cheng, Jianlin] Univ South China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Zhan, Xiaozhu] Sanming Supervis & Testing Ctr Qual & Safety Agro, Sanming 365000, Fujian, Peoples R China.
通讯机构：
[Deng, Jian] U;Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Alternating trilinear decomposition;Crystal violet;Dispersive liquid-liquid microextraction;Leucocrystal violet;Leucomalachite green;Malachite green;Parallel factor analysis
摘要：
A novel spectrofluorimetric method has been developed for the simultaneous determination of leucomalachite green (LMG), leucocrystal violet (LCV), malachite green (MG) and crystal violet (CV) by combining the sensitivity of molecular fluorescence and the selectivity of the second-order calibration. Residues of LMG, LCV, MG and CV were simultaneously extracted from fish and shrimp muscle with acetonitrile. The non-fluorescent CV and MG were then reduced to the corresponding fluorescent LMG and LCV by reacting with sodium borohydride. After preconcentration with dispersive liquid-liquid microextraction technique, the extracts were analyzed by using excitation-emission matrix fluorescence coupled with second-order calibration methods based on parallel factor analysis (PARAFAC) and alternating trilinear decomposition (ATLD) algorithms. The limits of detection obtained were 2.21-2.65 ng g-1 by PARAFAC and 2.30-2.86 ng g-1 by ATLD, respectively. The developed method was successfully applied to simultaneous determination of the four analytes in grass carp and shrimp samples with recoveries of 90.53-103.03% for PARAFAC and 90.40-102.75% for ATLD. The accuracy of this novel method was also verified by high performance liquid chromatography. © 2015 Elsevier Ltd.
语种：
英文
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A novel core–shell magnetic nano-sorbent with surface molecularly imprinted polymer coating for the selective solid phase extraction of dimetridazole
作者：
Hu, Canhui;Deng, Jian* ;Zhao, Yubao;Xia, Liangshu;Huang, Kaihui;...
期刊：
Food Chemistry ,2014年158:366-373 ISSN：0308-8146
通讯作者：
Deng, Jian
作者机构：
[Huang, Kaihui; Xiao, Ni; Zhao, Yubao; Hu, Canhui; Deng, Jian; Ju, Saiqin; Xia, Liangshu] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Deng, Jian] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Dimetridazole;Magnetic nano-sorbent;Sol-gel process;Surface molecularly imprinted polymer
摘要：
A novel core-shell magnetic nano-sorbent with surface molecularly-imprinted polymer coating was prepared via a sol-gel process. Methyltrimethoxysilane and 3-aminopropyltriethoxysilane were used as functional monomers, tetraethyl orthosilicate as cross-linker, and Al3+ as dopant to generate Lewis acid sites in the silica matrix for the metal coordinate interactions with the template dimetridazole (DMZ). The ratios of the monomers, the dopant, and the cross-linker, were optimised by a OA9 (34) orthogonal array design. The resultant sorbent was characterised by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, powder X-ray diffraction, and magnetometry. The binding performances of the sorbent were evaluated by static, kinetic and selective adsorption experiments. The nano-sorbent was successfully applied to solid phase extraction followed by spectrophotometric determination of DMZ in real samples. Spiked recoveries ranged from 90.33% to 106.20% for egg, milk powder, and pig feed samples, with relative standard deviations of less than 4.54%. © 2014 Elsevier Ltd. All rights reserved.
语种：
英文
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Application of derivative and derivative ratio spectrophotometry to simultaneous trace determination of rhodamine B and rhodamine 6G after dispersive liquid-liquid microextraction
作者：
Xiao, Ni;Deng, Jian* ;Huang, Kaihui;Ju, Saiqin;Hu, Canhui;...
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2014年128:312-318 ISSN：1386-1425
通讯作者：
Deng, Jian
作者机构：
[Xiao, Ni; Huang, Kaihui; Hu, Canhui; Deng, Jian; Ju, Saiqin; Liang, Jun] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Deng, Jian] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Rhodamine B;Rhodamine 6G;Derivative spectrophotometry;Derivative ratio spectrophotometry;Dispersive liquid-liquid microextraction;Food samples
摘要：
Two novel methods, first derivative spectrophotometric method ( <sup>1</sup>D) and first derivative ratio spectrophotometric method ( <sup>1</sup>DR), have been developed for the simultaneous trace determination of rhodamine B (RhB) and rhodamine 6G (Rh6G) in food samples after dispersive liquid-liquid microextraction (DLLME). The combination of derivative spectrophotometric techniques and DLLME procedure endows the presented methods with enhanced sensitivity and selectivity. Under optimum conditions, the linear calibration curves ranged from 5 to 450 ng mL<sup>-1</sup>, with the correlation coefficients (r) of 0.9997 for RhB and 0.9977 for Rh6G by <sup>1</sup>D method, and 0.9987 for RhB and 0.9958 for Rh6G by <sup>1</sup>DR method, respectively. The calculated limits of detection (LODs) based on the variability of the blank solutions (S/N = 3 criterion) for 11 measurements were in the range of 0.48-1.93 ng mL<sup>-1</sup>. The recoveries ranged from 88.1% to 111.6% (with RSD less than 4.4%) and 91.5-110.5% (with RSD less than 4.7%) for <sup>1</sup>D and <sup>1</sup>DR method, respectively. The influence of interfering substances such as foreign ions and food colorants which might be present in the food samples on the signals of RhB and Rh6G was examined. The developed methods have been successfully applied to the determination of RhB and Rh6G in black tea, red wine and chilli powder samples with the characteristics of simplicity, cost-effectiveness, environmental friendliness, and could be valuable for routine analysis. ©2014 Elsevier B.V. All rights reserved.
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英文
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医用有机化学理论教学改革的探索与实践
作者：
梁俊;邓健;罗虹;魏传晚;何军
期刊：
山西医科大学学报 ,2014年16(6):437-439 ISSN：1007-6611
作者机构：
南华大学化学化工学院基础化学教研室,衡阳,421001
关键词：
医用有机化学;教学改革;教学方法
摘要：
为了提高医学专业学生的学习兴趣和培养学生的创新能力,全面提升医用有机化学的教学质量。通过医用有机化学理论教学改革,从注重理论联系实际,精心组织教学内容;优化教学设计,合理使用多媒体课件进行教学;培养学生的自主性学习等方面进行了探索和实践。
语种：
中文
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研讨式教学方法在医用有机化学教学中的应用
作者：
何军;梁俊;魏传晚;王宏青;邓健;...
期刊：
广州化工 ,2014年(17):211-212,218 ISSN：1001-9677
作者机构：
南华大学化学化工学院化学教研室,湖南 衡阳,421001
关键词：
研讨式教学法;医学有机化学;启发式知识传授;互动式讨论
摘要：
有机化学是医学专业重要基础课程。随着教育教学改革的发展,使得医学有机化学的教学面临不少困境。本文从分析教学现状入手,分析问题,提出具体的解决方法,尝试用研讨式教学方法进行医学有机化学的教学改革。采取启发式知识传授、互动式讨论、课题式研讨、研究性实验、归纳性总结等几个环节,激发学生的学习兴趣,提高学生的综合素质。取得了良好的教学效果。
语种：
中文
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Chiral Separation of Racemic Naproxen by High-Performance Liquid Chromatography with β-CD/SiO2 as the Chiral Stationary Phase
作者：
Wang, Lili;Deng, Jian* ;Ji, Xuezhen;Liu, Wanbin;Liang, Jun;...
期刊：
JOURNAL OF AOAC INTERNATIONAL ,2014年97(1):121-127 ISSN：1060-3271
通讯作者：
Deng, Jian
作者机构：
[Wang, Lili; Yan, Xiaona; Xie, Jie; Chen, Dan; Deng, Jian; Ji, Xuezhen; Liang, Jun] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Liu, Wanbin] Henan Pingdingshan Qual & Technol Inspect, Pingdingshan 467000, Henan, Peoples R China.
通讯机构：
[Deng, Jian] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
摘要：
A novel derivative β-cyclodextrin (β-CD) bonded silica gel (β-CD/SiO2) was prepared via carbodiimide activation and used as a chiral stationary phase (CSP). The β-CD/SiO2 CSP was characterized by X-ray photoelectron spectroscopy and FTIR spectroscopy to prove the successful immobilization of β-CD onto the surface of the silica gel. The thermal behavior of the CSP was studied by differential scanning calorimetry and then used for the chiral separation of racemic naproxen using HPLC. Several factors affecting the separation efficiency of naproxen enantiomers were investigated systematically. The following parameters were selected: methanol–0.01 M phosphate buffer solution (85 + 15, v/v, pH 3.5) was the mobile phase, the flow rate was 1.0 mL/min, and the column temperature was 35°C. Under optimal conditions, the racemic naproxen was efficiently separated with a resolution factor (R S) of 1.70 and a separation factor (α) of 1.25. This method was successfully used for the enantiomeric separation and purity assessment of naproxen in commercial naproxen sustained release tablets.
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英文
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