A highly sensitive fluorescence probe for metallothioneins based on tiron–copper complex
作者：
Xiao, Xilin* ;Xue, Jinhua;Liao, Lifu;Huang, Mingyang;Zhou, Bin;...
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2015年145:85-89 ISSN：1386-1425
通讯作者：
Xiao, Xilin
作者机构：
[Zhou, Bin; Huang, Mingyang; Xiao, Xilin; Liao, Lifu; Xue, Jinhua; He, Bo] Univ South China, Inst Pathogen Biol, Coll Publ Hlth, Coll Chem & Chem Engn, Hengyang City 421001, Hunan Province, Peoples R China.
通讯机构：
[Xiao, Xilin] U;Univ South China, Inst Pathogen Biol, Coll Publ Hlth, Coll Chem & Chem Engn, Hengyang City 421001, Hunan Province, Peoples R China.
关键词：
Metallothioneins;Tiron-copper complex;Fluorescence probe
摘要：
The fabrication of tiron-copper complex as a novel fluorescence probe for the sensitive directly detection of metallothioneins at nanomolar levels was demonstrated. In Britton-Robinson (B-R) buffer (pH 7.50), the interaction of bis(firon)copper(II) complex cation [Cu(tiron)(2)](2+) and metallothioneins enhanced the fluorescence intensity of the system. The fluorescence enhancement at 347 nm was proportional to the concentration of metallothioneins. The mechanism was studied and discussed in terms of the fluorescence spectra. Under the optimal experimental conditions, at 347 nm, there was a linear relationship between the fluorescence intensity and the concentration of the metallothioneins in the range of 8.80 x 10(-9)-7.70 x 10(-7) mol L-1, with a correlation coefficient of r = 0.995 and detection limit 2.60 x 10(-9) mol L-1. The relative standard deviation was 0.77% (n = 11), and the average recovery 94.4%. The method proposed was successfully reliable, selective and sensitive in determining of trace metallothioneins in fish visceral organ samples with the results in good agreement with those obtained by HPLC. (C) 2015 Elsevier B.V. All rights reserved.
语种：
英文
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Colorimetric determination of 8-hydroxy-2'-deoxyguanosine using label-free aptamer and unmodified gold nanoparticles
作者：
Wang, Jia-Cheng;Wang, Yong-Sheng;Rang, Wei-Qing;Xue, Jin-Hua;Zhou, Bin;...
期刊：
Microchimica Acta ,2014年181(9-10):903-910 ISSN：0026-3672
通讯作者：
Wang, Yong-Song
作者机构：
[Rang, Wei-Qing; Zhou, Bin; Wang, Yong-Song; Yin, Ji-Cheng; Qian, Qiu-Mei; Xue, Jin-Hua; Wang, Yong-Sheng; Liu, Lu; Wang, Jia-Cheng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Song] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Aptamer;Gold nanoparticles;8-Hydroxy-2 '-deoxyguanosine;Colorimetric sensing
摘要：
We report on a sensitive and specific method for the visual and colorimetric detection of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in human urine by using the 8-OHdG-aptamer (Apt) as the recognition element. The aptamer was adsorbed on the surface of gold nanoparticles which enhances their stability. On addition of 8-OHdG, the conformation of the aptamer changes to form a G-quadruplex structure, thereby losing the ability to protect the nanoparticles and causing a color change from red to blue. The conformational changes were also studied by circular dichroism. The response is linearly dependent on the concentration of 8-OHdG in the range from 5.6 to 282 nM, the limit of detection is 1.7 nM, the relative standard deviation ranges from 1.1 to 4.2 % (for n = 6), and the recoveries range from 95.9 to 104.9 %. The method paves the way for visual analysis of samples without the use of costly instruments. [Figure not available: see fulltext.] © 2014 Springer-Verlag Wien.
语种：
英文
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水中痕量镉的曙红Y-中性红能量转移荧光猝灭法测定
作者：
肖锡林;薛金花;廖力夫;肖明春;杜可杰;...
期刊：
南华大学学报(自然科学版) ,2014年(1):91-96 ISSN：1673-0062
作者机构：
南华大学 化学化工学院,湖南 衡阳,421001;南华大学 公共卫生学院,湖南 衡阳,421001
关键词：
曙红Y;中性红;镉离子;能量转移荧光猝灭法
摘要：
在pH 7.9的BR缓冲液中,曙红Y-中性红和镉离子相互作用,在535 nm处荧光强度随着镉浓度的增加,体系的荧光逐渐降低,其荧光强度降低值和镉离子含量在一定范围内有相关关系.据此建立了一种测定痕量镉的曙红Y-中性红体系能量转移荧光猝灭新方法,并优化了实验条件.在优化的实验条件下,体系在535 nm处的荧光强度降低值与镉的摩尔浓度在1.6＆#215;10-8~3.2＆#215;10-6 mol/L范围内线性关系良好,其线性回归方程为：？F=936.60-2425.2c（＆#215;10-6mol/L）,相关系数r=0.998,检出限为4.7＆#215;10-9 mol/L,相对标准偏差是0.45%,加标回收率是90.75%.本方法操作简便、灵敏、选择性好,用于实际样品中镉的测定,其测定结果同石墨炉原子吸收法一致（t检验法）.
语种：
中文
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An ultrasensitive label-free assay of 8-hydroxy-2′-deoxyguanosine based on the conformational switching of aptamer
作者：
Wang, Jia-Cheng;Wang, Yong-Sheng;Xue, Jin-Hua;Zhou, Bin;Qian, Qiu-Mei;...
期刊：
Biosensors and Bioelectronics ,2014年58:22-26 ISSN：0956-5663
通讯作者：
Wang, Yong-Song
作者机构：
[Zhou, Bin; Wang, Yong-Song; Yin, Ji-Cheng; Qian, Qiu-Mei; Zhao, Hui; Liu, Hui; Liu, Shan-Du; Xue, Jin-Hua; Wang, Yong-Sheng; Wang, Jia-Cheng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Wang, Jia-Cheng] Dali Univ, Coll Publ Hlth, Dali 671000, Peoples R China.
通讯机构：
[Wang, Yong-Song] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
8-hydroxy-2'-deoxyguanosine;Aptamer;Conformational switching;Gold nanoparticles;Resonance light scattering;Ultrasensitive assay
摘要：
We developed a highly sensitive label-free assay of 8-hydroxy-2'-deoxyguanosine (8-OHdG) using 8-OHdG-aptamer (Apt) as the recognition element. The Apt was adsorbed onto the surface of gold nanoparticles (AuNPs), which prevents them from aggregating under high-salt conditions. Upon addition of 8-OHdG, the conformation of the Apt changes to form a G-quadruplex structure, which leads to the aggregation of the AuNPs along with the increase of the resonance light scattering intensity. The mechanism of 8-OHdG that induces Apt to form G-quadruplexes structure was studied by circular dichroism. The response signals linearly correlated with the concentration of 8-OHdG ranging from 90.8. pM to 14.1. nM with a detection limit of 27.3. pM, which is much lower than that obtained by other methods. This method does not need any label steps and sophisticated equipment. The application for detection of 8-OHdG in real samples further demonstrated its reliability. This strategy would be helpful for developing a universal analytical method by simply replacing corresponding aptamers for various target molecules. © 2014 Elsevier B.V.
语种：
英文
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A sensitive resonance light scattering assay for uranyl ion based on the conformational change of a nuclease-resistant aptamer and gold nanoparticles acting as signal reporters
作者：
Zhou, Bin;Wang, Yong-Sheng* ;Yang, Hui-Xian;Xue, Jin-Hua;Wang, Jia-Cheng;...
期刊：
Microchimica Acta ,2014年181(11-12):1353-1360 ISSN：0026-3672
通讯作者：
Wang, Yong-Sheng
作者机构：
[Zhou, Bin; Yang, Hui-Xian; Zhao, Hui; Liu, Shan-Du; Liu, Hui; Xue, Jin-Hua; Wang, Yong-Sheng; Wang, Jia-Cheng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Nuclease-resistant DNA aptamer;Gold nanoparticles;Resonance light scattering;Conformation change;Uranyl
摘要：
Various methods have been developed in recent years for the determination of uranyl ion by making use of uranyl-specific DNAzymes. However, many of them suffer from hydrolysis by nucleases present in samples such as body fluids. We report here on an uranyl-specific nuclease-resistant DNA aptamer (UApt) as the recognition element, and how gold nanoparticles (AuNPs) can be used as signal reporters in the respective assay. The presence of uranyl ion leads to a conformational change of UApt, and this results in the dispersion of AuNPs and a decrease in the intensity of resonance light scattering (RLS) at around 573.0 nm. The conformational changes were also studied by polyacrylamide gel electrophoresis, circular dichroism, and UV–vis spectroscopy. The RLS signals are linearly related to the concentration of uranyl ion in the 22 to 550 nM range, with a detection limit of 6.7 nM. This method is more simple and robust than others owing to use of a UApt without a ribonucleotide adenosine. It has been successfully applied to the determination of uranyl ion in real samples. We presume that this method may be extended to the determination of other analytes by making use of the corresponding aptamer for the target. The presence of UO22+ leads to the conformation change of UApt, resulting in the dispersion of AuNPs and decrease of the RLS intensity of the system.
语种：
英文
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水中痕量铜的中性红－曙红Y同步荧光猝灭法检测
作者：
肖锡林;薛金花;廖力夫;贺冬秀;王芳;...
期刊：
分析仪器 ,2014年(6):24-29 ISSN：1001-232X
作者机构：
南华大学化学化工学院，南华大学公共卫生学院，衡阳 421001;江西中医学院现代中药制剂教育部重点实验室,南昌,330004;[薛金花; 何博; 王芳; 肖锡林; 贺冬秀; 廖力夫] 南华大学;[袁金斌] 江西中医学院
关键词：
曙红Y;中性红;铜;同步荧光猝灭
摘要：
在pH8.5的T ris－HCl缓冲液中，曙红Y －中性红和Cu（Ⅱ）相互作用，在538nm处随着Cu（Ⅱ）浓度的增加，体系同步荧光强度逐渐降低，同步荧光强度降低值和Cu（Ⅱ）含量在一定范围内有相关关系。据此应用中性红－曙红Y同步荧光猝灭法建立一种测定铜的新方法。在优化的实验条件下，体系在538nm处的荧光强度降低值与铜的摩尔浓度在7.61＆#215;10－8－3.60＆#215;10－6 mol ＆#183; L －1范围内线性关系良好，其线性回归方程为：△ F＝－0.414＋314.89c（＆#215;10－6 mol ＆#183; L －1），相关系数 r＝0.998，检出限为2.48＆#215;10－8 mol ＆#183; L －1，相对标准偏差是0.26％，加标回收率是101.12％。本方法操作简便、灵敏、选择性好，可用于实际样品中铜的测定。
语种：
中文
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A colorimetric aptasensor for the highly sensitive detection of 8-hydroxy-2′-deoxyguanosine based on G-quadruplex–hemin DNAzyme
作者：
Liu, Hui;Wang, Yong-Sheng* ;Wang, Jia-Cheng;Xue, Jin-Hua;Zhou, Bin;...
期刊：
Analytical Biochemistry ,2014年458:4-10 ISSN：0003-2697
通讯作者：
Wang, Yong-Sheng
作者机构：
[Zhou, Bin; Chen, Si-Han; Li, Ming-Hui; Tang, Xian; Cao, Jin-Xiu; Zhao, Hui; Liu, Hui; Liu, Shan-Du; Xue, Jin-Hua; Wang, Yong-Sheng; Wang, Jia-Cheng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Wang, Jia-Cheng] Dali Univ, Coll Publ Hlth, Dali 671000, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
8-Hydroxy-2'-deoxyguanosine;Colorimetric aptasensor;Conformational switching;G-quadruplex;Hemin
摘要：
A highly sensitive, low-cost colorimetric aptasensor was developed for the determination of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in human urine. The method is based on a conformational switching of the 8-OHdG aptamer to form a G-quadruplex structure in the presence of 8-OHdG. The resulting G-quadruplex assembles into a peroxidase-like DNAzyme with hemin, which effectively catalyzes the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS2-) by H2O2 to ABTS +, resulting in an increase in the absorption signal at 416 nm along with a color change of the solution. The response signals linearly correlated with the concentration of 8-OHdG, ranging from 466 pM to 247 nM with a detection limit of 141 pM. The relative standard deviation and the recovery were 1.97-3.47% (n = 11) and 98.8-100.2%, respectively. The proposed method avoids the label and derivatization steps in common methods and allows direct analysis of the samples by the naked eye without costly instruments, which is reliable, inexpensive, and sensitive. © 2014 Elsevier Inc. All rights reserved.
语种：
英文
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Fluorescence quenching determination of metallothioneins using 8-hydroxyquinoline-5-sulphonic acid-Cd(II) chelate
作者：
Qian, Qiu-Mei;Wang, Yong-Sheng;Zhou, Bin;Xue, Jin-Hua;Li, Le;...
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2014年118:992-998 ISSN：1386-1425
通讯作者：
Wang, Yong-Song
作者机构：
[Zhou, Bin; Wang, Yong-Song; Yin, Ji-Cheng; Qian, Qiu-Mei; Li, Le; Zhao, Hui; Liu, Shan-Du; Liu, Hui; Xue, Jin-Hua; Wang, Yong-Sheng; Wang, Jia-Cheng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Song] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Metallothioneins;8-Hydroxyquinoline-5-sulphonic acid;Cadmium;Fluorescence quenching
摘要：
A novel method for the determination of metallothioneins (MTs) in urine was developed by fluorescence quenching strategy. The response signals linearly correlated with the concentration of MTs in the ranges of 3.12 ×10 <sup>-8</sup>-1.23 ×10<sup>-6</sup> mol L<sup>-1</sup>, and the limit of detection (LOD) was 9.36 ×10<sup>-9</sup> mol L<sup>-1</sup>. The proposed method avoids the label and derivatization steps in common methods, and is reliable, inexpensive and sensitive. Furthermore, the interaction of MTs and 8-hydroxyquinoline-5-sulphonic acid (HQS)-Cd(II) chelate was investigated, and a static quenching mode was proposed to be primarily responsible for the fluorescence quenching event. It could provide a promising potential for the detection of the biomacromolecules which have no native fluorescence, and be benefit to extend the application of fluorescence strategy. ©2013 Elsevier B.V. All rights reserved.
语种：
英文
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水中痕量镉(Ⅱ)的荧光素-曙红Y能量转移荧光猝灭测定法
作者：
肖锡林;薛金花;廖力夫;赵睿;周斌;...
期刊：
环境与健康杂志 ,2014年31(6):525-527 ISSN：1001-5914
作者机构：
南华大学化学化工学院,湖南衡阳421001;[薛金花; 赵睿; 周斌] 南华大学公共卫生学院;[肖锡林; 廖力夫; 何博] 南华大学化学化工学院
关键词：
荧光素;曙红Y;能量转移;荧光猝灭法;镉(Ⅱ)
摘要：
目的建立水中痕量镉(Ⅱ)的荧光素-曙红Y能量转移荧光猝灭测定法。方法取0.50ml的水样置于5.0 ml的比色管中,分别加入1×10~(-4) mol/L荧光素溶液0.30 ml,1×10~(-5) mol/L曙红Y溶液0.10 ml,BR缓冲液(pH 6.5)0.50 ml,定容后在502 nm处测定溶液荧光强度(F)值,以内标法定量。结果在3.43×10~(-7) ~1.246×10~(-5) mol/L的线性范围为,该方法所得镉(Ⅱ)的线性回归方程为ΔF=14.221+546.81×10~(-5)c,r=0.999 5。该方法的检出限为1.03×10~(-8) mol/L,平均回收率为94.53%~98.15%,RSD为2.44%~4.67%。结论该方法操作简便、灵敏、选择性好,适用于水中镉(Ⅱ)的测定。
语种：
中文
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Determination of trace metallothioneins at nanomolar levels using phenanthroline-copper coordination by fluorescence spectra
作者：
Xiao, Xilin* ;Xue, Jinhua;Liao, Lifu;Chen, Xiangcheng;Zeng, Yanhua;...
期刊：
Analytical Sciences ,2014年30(10):999-1004 ISSN：0910-6340
通讯作者：
Xiao, Xilin
作者机构：
[Chen, Xiangcheng; Wu, Yimou; Xiao, Xilin; Liao, Lifu; Xue, Jinhua; Zeng, Yanhua] Univ South China, Coll Chem & Chem Engn, Coll Publ Hlth, Inst Pathogen Biol, Hengyang City 421001, Hunan, Peoples R China.
通讯机构：
[Xiao, Xilin] U;Univ South China, Coll Chem & Chem Engn, Coll Publ Hlth, Inst Pathogen Biol, Hengyang City 421001, Hunan, Peoples R China.
关键词：
Metallothioneins;copper;1,10-phenanthroline;fluorescence
摘要：
A direct fluorescence spectra method was applied for the determination of metallothioneins at nanomolar levels. In Britton-Robison (B-R) buffer (pH 7.0), the interaction of bis(1,10-phenanthroline)copper(II) complex cation [Cu(phen)2]2+ and metallothioneins enhanced the fluorescence intensity of system. The fluorescence enhancement at 365 nm was proportional to the concentration of metallothioneins. The mechanism was studied and discussed in terms of the fluorescence and UV-absorption spectra. Under the optimal experimental conditions, at 365 nm, there was a linear relationship between the fluorescence intensity and the concentration of the metallothioneins in the range of 8.30 × 10-9 - 7.70 × 10-7 mol L-1. The linear regression equation was ΔF = 8.96 + 38.01c (mol L-1), with a correlation coefficient of r = 0.998 and detection limit 2.50 × 10-9 mol L-1. The relative standard deviation was 0.47% (n = 11), and the average recovery 97.2%. The proposed method was successfully reliable, selective and sensitive in determining trace metallothioneins in fish visceral organ samples with the results in good agreement with those obtained by HPLC. © The Japan Society for Analytical Chemistry.
语种：
英文
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Determination of metallothioneins by fluorescence and resonance light scattering strategies based on ciprofloxacin–Cu(II) system
作者：
Liu, Lu;Wang, Yong-Sheng;Xue, Jin-Hua;Yang, Hui-Xian;Li, Qiu;...
期刊：
Journal of Luminescence ,2013年138:251-257 ISSN：0022-2313
通讯作者：
Wang, Yong-Song
作者机构：
[Zhou, Bin; Wang, Yong-Song; Yang, Hui-Xian; Yin, Ji-Cheng; Xue, Jin-Hua; Liu, Lu; Wang, Yong-Sheng; Li, Qiu; Wang, Jia-Cheng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Song] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Metallothioneins;Ciprofloxacin-copper chelate;Fluorescence;Resonance light scattering
摘要：
Based on ciprofloxacin (CIP)-Cu(II) system, the novel methods for the detection of metallothioneins (MTs) have been developed by fluorescence (FL) and resonance light scattering (RLS) strategies. The FL strategy avoids the label and derivatization steps in common methods, while the RLS strategy can be applied for determining bio-macromolecules and small molecules without native fluorescence. The response signals linearly correlated with the concentration of MTs over the ranges of 1.03×10<sup>-8</sup>-1.23×10<sup>-6</sup> mol L<sup>-1</sup> for FL, and of 2.56×10<sup>-7</sup>-1.54×10 <sup>-6</sup> mol L<sup>-1</sup> for RLS. The limits of detection (LOD) are 3.1×10<sup>-9</sup> mol L<sup>-1</sup> for FL and 7.68×10 <sup>-8</sup> mol L<sup>-1</sup> for RLS. This study represents the comparison of these two methods using the same CIP-Cu<sup>2+</sup>-MTs system. They not only allow practical application for MTs detection but also serve as a potential choice for the operators according to their concrete needs. In addition, the mechanisms for FL and RLS enhancement of the system were also discussed. ©2013 Elsevier B.V.
语种：
英文
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Resonance light scattering determination of metallothioneins using levofloxacin−palladium complex as a light scattering probe
作者：
Xue, Jin-Hua;Qian, Qiu-Mei;Wang, Yong-Sheng* ;Meng, Xia-Ling;Liu, Lu
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2013年102:205-211 ISSN：1386-1425
通讯作者：
Wang, Yong-Sheng
作者机构：
[Qian, Qiu-Mei; Xue, Jin-Hua; Wang, Yong-Sheng; Liu, Lu; Meng, Xia-Ling] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Levofloxacin hydrochloride;Metallothioneins;Palladium;Resonance light scattering
摘要：
A novel method of resonance light scattering (RLS) was developed for the analysis of trace metallothioneins (MTs) in human urine. In a CH <inf>3</inf>COOH-CH<inf>3</inf>COONa buffer solution of pH 4.5, the formation of a complex between levofloxacin (LEV)-Pd and MTs led to enhance the RLS intensity of the system, and the enhanced RLS intensity at 468 nm was proportional to the concentration of MTs in the range of 0.059-22.4 μg mL<sup>-1</sup>. The linear regression equation was ΔI = 127.5 ρ(μg mL<sup>-1</sup>)-88.02 with a correlation coefficient of 0.9992, and the detection limit of 17.8 ng mL<sup>-1</sup>. The relative standard deviation and the average recovery were 3.8-5.4% (n = 11) and 92.15%, respectively. The proposed method is convenient, reliable and sensitive, and has been used successfully for the determination of trace MTs in human urine samples. ©2012 Elsevier B.V. All rights reserved.
语种：
英文
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“卓医计划”背景下放射医学实验教学的思考与实践
作者：
肖方竹;何爱桃;薛金花;李乐;唐艳;...
期刊：
医学教育研究与实践 ,2013年21(5):952-954 ISSN：2096-3181
作者机构：
南华大学公共卫生学院,湖南衡阳,421001;[何淑雅; 李乐; 唐艳; 薛金花; 何爱桃; 肖方竹] 南华大学
关键词：
卓医计划;放射医学;实验教学;优化;创新
摘要：
"卓越医生教育培养计划"是国家深化医药卫生体制改革的重大项目,是推进医学院校临床医学教育综合改革、创新人才培养模式、提高人才培养质量的有效途径。本文从放射医学实验课程体系的优化、人才培养模式的改革以及实验教学管理模式的创新等方面进行分析与探讨。
语种：
中文
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不同剂量辐照黄花菜保鲜研究
作者：
郑贤利;屈国普;谢红艳;薛金花
期刊：
安徽农业科学 ,2013年41(11):5032-5033 ISSN：0517-6611
作者机构：
湖南省南华大学核科学技术学院,湖南衡阳,421001;湖南省南华大学公共卫生学院,湖南衡阳,421001;湖南省南华大学药学与生物科学学院,湖南衡阳,421001
关键词：
黄花菜;剂量;剂量率;营养成分;辐照保鲜
摘要：
[目的]研究辐照对鲜黄花菜的保鲜效果。[方法]鲜黄花菜通过包装处理后用60Coγ射线辐照,分析不同剂量和剂量率对其营养成分的影响以及保鲜效果。[结果]鲜黄花菜经不同剂量的60Coγ射线辐照后其营养成分没有明显的变化,同一剂量不同吸收剂量率对鲜黄花菜中的营养成分的影响几乎是一样的,低剂量辐照黄花菜保鲜效果优于高剂量辐照。低剂量辐照加低温贮存,保鲜效果优于常温贮存。[结论]研究可为辐照应用于黄花菜的保鲜提供参考。
语种：
中文
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A wireless magnetoelastic sensor for uranyl using DNAzyme–graphene oxide and gold nanoparticles-based amplification
作者：
Yin, Ji-Cheng;Wang, Yong-Sheng;Zhou, Bin;Xiao, Xi-Lin;Xue, Jin-Hua;...
期刊：
Sensors and Actuators B-Chemical ,2013年188:147-155 ISSN：0925-4005
通讯作者：
Wang, Yong-Song
作者机构：
[Zhou, Bin; Wang, Yong-Song; Yin, Ji-Cheng; Qian, Qiu-Mei; Xue, Jin-Hua; Wang, Yong-Sheng; Wang, Jia-Cheng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Song] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
DNAzyme;Gold nanoparticles;Graphene oxide;Uranyl;Wireless magnetoelastic sensor
摘要：
A wireless magnetoelastic sensor (WMS) for uranyl detection has been developed using DNAzyme-graphene oxide (GO) and gold nanoparticles (AuNPs)-based amplification. The WMS is fabricated by immobilizing a layer of GO film on a magnetoelastic ribbon-like sensor. When the substrate strand of DNAzyme was cleaved in the presence of UO<inf>2</inf><sup>2+</sup>, the single-stranded DNA (ssDNA) was adsorbed onto the GO film of the WMS, resulting in a decrease of resonance frequency of the WMS. HAuCl<inf>4</inf> can be reduced by ascorbic acid (AA) to atom Au coated on AuNPs in cetyltrimethyl ammonium bromide (CTMAB) solution, leading to the signal amplification of the system. When the molar ratio of AA and HAuCl<inf>4</inf> was 3:2, the response signals linearly correlated with the concentration of UO<inf>2</inf><sup>2+</sup> over the range of 9.57 ×10<sup>-9</sup> to 1.20 ×10<sup>-7</sup> M in the MES buffer solution of pH 5.0 with a detection limit of 2.87 ×10<sup>-9</sup> M. The proposed WMS has additional advantages of being less time-consuming, low cost and remote query, and avoids the interference of some metals such as Cu<sup>2+</sup>, Fe<sup>2+</sup>, Fe<sup>3+</sup>, Hg<sup>2+</sup>, and Tb <sup>3+</sup>. It has been successfully applied for detecting UO <inf>2</inf><sup>2+</sup> in real samples with a satisfactory result. A polyacrylamide gel electrophoresis (PAGE) assay, scanning electron microscopy (SEM) and UV-vis spectra were used to confirm the mechanism for structure switching and signal amplification. ©2013 Elsevier B.V.
语种：
英文
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Resonance light scattering determination of uranyl based on labeled DNAzyme-gold nanoparticle system
作者：
Zhou, Bin;Shi, Lin-Fei;Wang, Yong-Sheng;Yang, Hui-Xian;Xue, Jin-Hua;...
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2013年110:419-424 ISSN：1386-1425
通讯作者：
Wang, Yong-Song
作者机构：
[Zhou, Bin; Wang, Yong-Song; Yang, Hui-Xian; Yin, Ji-Cheng; Shi, Lin-Fei; Xue, Jin-Hua; Wang, Yong-Sheng; Liu, Lu; Wang, Jia-Cheng] Univ South China, Coll Publ Hlth, Hengyang 427001, Peoples R China.
通讯机构：
[Wang, Yong-Song] U;Univ South China, Coll Publ Hlth, Hengyang 427001, Peoples R China.
关键词：
DNAzyme/Gold nanoparticles;Resonance light scattering;Uranyl
摘要：
A resonance light scattering (RLS) method has been developed using a uranyl (UO22+) specific DNAzyme and gold nanoparticles (AuNPs). In this strategy, the cleavage of the substrate strand (SDNA) of DNAzyme results in releasing a shorter duplex in the presence of UO22+, leading to the aggregation of AuNPs and the increase of RLS intensity. The response signals linearly correlated with the concentration of UO22+ over the range of 1.36 ×10<sup>-8</sup>-1.50 ×10<sup>-7</sup> mol L<sup>-1</sup>. The limit of detection (LOD) is 4.09 ×10<sup>-9</sup> mol L<sup>-1</sup>. The method has excellent selectivity and higher sensitivity. It could provide a promising potential for the detection of metal ions, and be benefit to extend the application of RLS method. ©2013 Elsevier B.V. All rights reserved.
语种：
英文
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Colorimetric detection of metallothioneins using a thymine-rich oligonucleotide–Hg complex and gold nanoparticles
作者：
Qian, Qiu-Mei;Wang, Yong-Sheng;Yang, Hui-Xian;Xue, Jin-Hua;Liu, Lu;...
期刊：
Analytical Biochemistry ,2013年436(1):45-52 ISSN：0003-2697
通讯作者：
Wang, Yong-Song
作者机构：
[Zhou, Bin; Wang, Yong-Song; Yang, Hui-Xian; Yin, Ji-Cheng; Qian, Qiu-Mei; Xue, Jin-Hua; Wang, Yong-Sheng; Liu, Lu; Wang, Jia-Cheng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Song] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Colorimetric sensing;Gold nanoparticles;Mercury;Metallothioneins;Thymine-rich oligonucleotide
摘要：
A simple and sensitive method for label-free, colorimetric detection of metallothioneins (MTs) has been developed by using a thymine (T)-rich oligonucleotide (TRO)-Hg-AuNP system. In this colorimetric strategy, the thiol groups of MTs could interact with mercury from the T-Hg2+-T complex to release TRO, resulting in a color change of the system. The response signals linearly correlated with the concentration of MTs over the range of 2.56 × 10-8 to 3.08 × 10-7 mol L-1, and the limit of detection was 7.67 × 10-9 mol L-1. The relative standard deviation and the recovery were 2.3-4.8% (n = 11) and 94.2-103.9%, respectively. The proposed method avoids the label and derivatization steps in common methods, allows direct analysis of the samples by the naked eye without costly instruments, and is reliable, inexpensive, and sensitive. © 2013 Elsevier Inc. All rights reserved.
语种：
英文
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水生捕食线虫真菌季节性分布及多样性研究
作者：
郝玉娥;张铭洋;谭胜全;周艺;薛金花;...
期刊：
南华大学学报(自然科学版) ,2012年26(1):87-92 ISSN：1673-0062
作者机构：
南华大学公共卫生学院,湖南衡阳,421001;湖南省怀化铁路疾病预防控制中心,湖南怀化,418000;湖南省益阳市沅江市疾控中心,湖南益阳,413100;[周艺; 全淑芬; 薛金花; 向艳; 郝玉娥] 南华大学;[张铭洋] 怀化铁路疾病预防控制中心
关键词：
水生捕食线虫真菌;季节分布;资源调查;多样性
摘要：
从云南滇池附近的三块水稻田和三口鱼塘中分离到的捕食线虫真菌种类比较相似，但从鱼塘中分离到的捕食线虫真菌种类要比水稻田中的少，两个生境中的优势种也不同．水稻田中，春季和秋季的优势种为Arbrostrysconoides，夏季和冬季的优势种为A．oligospora，秋季的捕食线虫真菌种类最多，春季次之，夏季和冬季最少；鱼塘中，春季、夏季和冬季的优势种均为Dactylellinaellipsosporum，秋季的优势种为A．vet-micola．夏季的捕食线虫真菌种类最多，其他三个季节的捕食线虫真菌种类相差不大．从湖南湘江衡阳段的300份泥土样品中，共分离、鉴定出17种水生捕食线虫真菌，东洲岛和新大桥水域分离的水生捕食线虫真菌比李坳水域分离的种数少．
语种：
中文
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环丙沙星-铜配合物紫外分光光度法测金属硫蛋白
作者：
刘璐;李秋;薛金花;周斌;王永生
期刊：
应用化工 ,2012年41(3):507-509 ISSN：1671-3206
作者机构：
南华大学公共卫生学院,湖南衡阳,421001
关键词：
环丙沙星;铜离子;金属硫蛋白;紫外可见分光光度法
摘要：
在pH=6的BR缓冲溶液中,环丙沙星-铜配合物与金属硫蛋白作用,导致体系吸光强度增强,在272 nm波长处,吸光强度与金属硫蛋白的浓度呈线性关系,据此建立了测定金属硫蛋白的新方法。方法的线性范围为0.259～1.54μmol/L,检出限7.76×10-8mol/L,样品平均加标回收率为91.8%。本方法操作简便、快速,灵敏,可用于实际样本测定,结果满意。
语种：
中文
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Spectroscopic Study on the Interaction of Pyronine Y with Nucleic Acids and Its Analytical Application
作者：
Xiao, Xilin;Huang, Mingyuan;Wang, Yongsheng* ;Gan, Lou;Zhu, Guizhi;...
期刊：
Spectroscopy Letters ,2012年45(8):569-574 ISSN：0038-7010
通讯作者：
Wang, Yongsheng
作者机构：
[Wang, Yongsheng; Xiao, Xilin; Xue, Jinhua] Univ S China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Wang, Yongsheng; Xiao, Xilin; Xue, Jinhua] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Huang, Mingyuan] Guangdong Med Coll, Sch Publ Hlth, Dongguan, Guangdong, Peoples R China.;[Gan, Lou] So Med Univ, Sch Publ Hlth & Trop Med, Guangzhou, Guangdong, Peoples R China.;[Zhu, Guizhi] Univ Florida, Dept Physiol, Shands Canc Ctr, UF Genet Inst, Gainesville, FL USA.
通讯机构：
[Wang, Yongsheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
关键词：
nucleic acids;pyronine Y;resonance Rayleigh scattering
摘要：
The interaction of pyronine Y (PY) with nucleic acids was studied by resonance Rayleigh scattering (RRS) for nucleic acid detection. The enhanced RRS intensity of nucleic acids reacted with PY was proportional to the concentration of nucleic acids in the ranges of 27.0-625ngml -1 for fish sperm DNA, 39.0-500ngml - for calf thymus DNA, and 59.0-375ngml -1 for yeast RNA. The limits of determination were 0.2ngml -1 for fish sperm DNA, 0.6ngml -1 for calf thymus DNA, and 0.7ngml 1 for yeast RNA. The method had been successfully applied to the quick determination of nucleic acids in synthetic and natural samples. © 2012 Copyright Taylor and Francis Group, LLC.
语种：
英文
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