Determination of Semicarbazide-Sensitive Amine Oxidase Activity in Blood Plasma by a Light Scattering Technique
作者：
Tan, Xuan;Rang, Wei-Qing;Wang, Yong-Sheng* ;Yang, Hui-Xian;Xue, Jin-Hua;...
期刊：
Analytical Letters ,2012年45(18):2774-2784 ISSN：0003-2719
通讯作者：
Wang, Yong-Sheng
作者机构：
[Rang, Wei-Qing; Zhou, Bin; Yang, Hui-Xian; Shi, Lin-Fei; Tan, Xuan; Xue, Jin-Hua; Yang, Hong-Mei; Wang, Yong-Sheng; Liu, Lu] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Tan, Xuan] Hengyang City Ctr Dis Prevent & Control, Hengyang, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
2,4-Dinitrophenylhydrazine;Benzaldehyde;Benzylamine;Light scattering;Semicarbazide-sensitive amine oxidase
摘要：
A novel method for the determination of semicarbazide-sensitive amine oxidase (SSAO) activity in blood plasma has been developed. The method was based on the change of light scattering (LS) intensity resulting from the derivative product of the interaction of 2,4-dinitrophenylhydrazine (DNPH) with benzaldehyde produced by catalyzing of SSAO to benzylamine. In Britton-Robinson (B-R) buffer solution, the intensity of system's LS at 514.6 nm was significantly enhanced and was directly proportional to the concentration of benzaldehyde. In this method, SSAO enzyme activity is defined as the concentration of benzaldehyde (nmol) formed per mL plasma per hour. The range of determination of SSAO enzyme activity was 6.40 × 10-3 -0.340 nmol mL-1 h-1 with a detection limit of 1.92 × 10-3 nmol mL-1 h-1. The relative standard deviation was 2.8-4.1% and the average recovery was 67.9% (n = 6). © 2012 Copyright Taylor and Francis Group, LLC.
语种：
英文
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A gold nanoparticles-modified aptamer beacon for urinary adenosine detection based on structure-switching/fluorescence-“turning on” mechanism
作者：
Zhang, Jin-Quan;Wang, Yong-Sheng* ;Xue, Jin-Hua;He, Yan;Yang, Hui-Xian;...
期刊：
Journal of Pharmaceutical and Biomedical Analysis ,2012年70:362-368 ISSN：0731-7085
通讯作者：
Wang, Yong-Sheng
作者机构：
[Yang, Hui-Xian; Shi, Lin-Fei; Zhang, Jin-Quan; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[He, Yan] Univ S China, Coll Life Sci & Technol, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin; Liang, Jun] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Adenosine;Aptamer beacon;Fluorescence-" turning on";Gold nanoparticles;Structure-switching
摘要：
A novel small molecule probe, aptamer beacon (AB), was introduced for adenosine (Ade) recognition and quantitative analysis. The Ade aptamer was engineered into an aptamer beacon by adding a gold nanoparticle-modified nucleotide sequence which is complementary to aptamer sequence (FDNA) at the 3'-end of FDNA. The fluorescence signal "turning on" was observed when AB was bound to Ade, which is attributed to a significant conformational change in AB from a FDNA/QDNA duplex to a FDNA-Ade complex. The Ade measurement was carried out in 20 mmol L-1 Tris-HCl buffer solution of pH 7.4, Delta F signal linearly correlated with the concentration of Ade over the range of 2.0 x 10(-8) to 1.8 x 10(-6) mol L-1. The limit of detection (LOD) for Ade is 6.0 x 10(-9) mol L-1 with relative standard deviations (R.S.D) of 3.64-5.36%, and the recoveries were 98.6%, 100%, 102% (n = 6), respectively. The present method has been successfully applied to determine Ade in human urine samples, and the obtained results were in good agreement with those obtained by the HPLC method. Our investigation shows that the unique properties of the AB could provide a promising potential for small molecules detection, and be benefit to extend the application of aptamer beacon technique. (C) 2012 Elsevier B.V. All rights reserved.
语种：
英文
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Determination of trace formaldehyde in blood plasma by resonance fluorescence technology
作者：
Wang, Yong-Sheng* ;Tan, Xuan;Xue, Jin-Hua;Li, Gui-Rong;Shi, Lin-Fei;...
期刊：
Analytica Chimica Acta ,2011年690(2):234-239 ISSN：0003-2670
通讯作者：
Wang, Yong-Sheng
作者机构：
[Zhou, Bin; Shi, Lin-Fei; Tan, Xuan; Li, Gui-Rong; Xue, Jin-Hua; Yang, Hong-Mei; Wang, Yong-Sheng; Liu, Lu] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Blood plasma;Formaldehyde;Potassium bromate;Pyronine Y;Resonance fluorescence
摘要：
A novel method for the determination of trace formaldehyde in blood plasma has been established by using resonance fluorimetry technique. It was based on the fact that oxidation of pyronine Y by potassium bromate was catalyzed by formaldehyde in sulfuric acid. When the wavelength interval was at Δλ=0nm, it was found that the decreased intensity (ΔF) of resonance fluorescence at 574.6nm was proportional to the concentration of formaldehyde in the range of 1.27×10-2 to 2.28μgmL-1. The limit of detection and the average recovery for formaldehyde were 3.80ngmL-1 and 101.6% (n=6), respectively. The present method had been applied to the determination of trace formaldehyde in blood plasma, and the obtained results were in good agreement with those obtained by the resonance light scattering method. © 2011 Elsevier B.V.
语种：
英文
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A Wireless magnetoelastic sensor for urinary 2-naphthol detection based on the precipitation of 2-naphthol with diazonium salts
作者：
Yuan, Yu-Kun;Wang, Yong-Sheng* ;Xiao, Xi-Lin;Xue, Jin-Hua;Li, Gui-Rong;...
期刊：
Analytical Sciences ,2011年27(5):517-521 ISSN：0910-6340
通讯作者：
Wang, Yong-Sheng
作者机构：
[Yuan, Yu-Kun; Shi, Lin-Fei; Kang, Rong-Hui; Li, Gui-Rong; Zhang, Jin-Quan; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Yuan, Yu-Kun] Chenzhou Fourth Peoples Hosp, Clin Lab, Chenzhou 423000, Peoples R China.;[Xiao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
摘要：
A wireless magnetoelastic sensor has been developed for the determination of 2-naphthol (2-NAP) in human urine. This method is based on the precipitation of 2-NAP with diazonium salts produced by the diazo-reaction of sulfamethoxazole (SMZ) with nitrite under a weak alkaline condition, resulting in a descending of the resonance frequency of a wireless magnetoelastic sensor. The frequence shift values (ΔF) of the sensor were directly proportional to the concentration in the range of 1.13 - 139 μmol L -1 for 2-NAP with a correlation coefficient of 0.997 and a detection limit of 0.340 μmol L -1 . The relative standard deviations were 2.38, 2.40 and 2.44%, and the average recovery was 107% (n = 6). The proposed method has additional advantages of being less time-consuming, low cost and remote query, and can be applied for realtime and in situ monitoring of 2-NAP in human urine. It would be a benefit to extend the scope of applications of magnetoelastic sensing techniques. 2011 © The Japan Society for Analytical Chemistry.
语种：
英文
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毛细管气相色谱测定染发剂中苯二胺
作者：
薛金花;梁超;李程
期刊：
应用化工 ,2011年40(2):349-352 ISSN：1671-3206
作者机构：
[薛金花; 梁超; 李程] 南华大学公共卫生学院
关键词：
气相色谱法;苯二胺;染发剂;毛细管柱
摘要：
建立了毛细管气相色谱法测定染发剂中邻,间,对苯二胺含量的分析方法.样品用乙酸乙酯,超声波提取,离心除去杂质,然后采用毛细管分离,FID检测,保留时间定性,峰面积定量.苯二胺三种异构体在0.0110 g/L浓度范围内线性关系良好,相关系数为0.999 50.999 6;邻苯二胺,对苯二胺,间苯二胺加标回收率分别为88.6%97.8%,93.3%105.0%,88.5%99.1%,相对标准偏差为1.94%2.05%(n=7),检出限分别为2,2.5,2 ng/mL.该方法简单,快速应用于实际样品测定,结果满意
语种：
中文
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Simultaneous determination of a-naphthol, b-naphthol and 1-hydroxypyrene in urine by synchronous fluorescence spectrometry using b-cyclodextrin as a sensitiser
作者：
Wang, Ying;Wang, Yong-Sheng* ;Yang, Hong-Mei;Xue, Jin-Hua;Tan, Xuan;...
期刊：
International Journal of Environmental Analytical Chemistry ,2011年91(1):87-96 ISSN：0306-7319
通讯作者：
Wang, Yong-Sheng
作者机构：
[Guo, Yu; Wang, Ying; Tan, Xuan; Yang, Hong-Mei; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Wang, Ying] Hengyand Blood Ctr, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
alpha-naphthol;beta-naphthol;1-hydroxypyrene;synchronous fluorescence;beta-cyclodextrin;double scans;urine
摘要：
A novel method for the simultaneous determination of alpha-naphthol (alpha-NAP), beta-naphthol (beta-NAP) and 1-hydroxypyrene (1-OHP) in human urine has been established by using synchronous fluorescence spectrometry. The measurement was carried out in sodium acetate-boroborax buffer solution (pH = 5.0) with beta-cyclodextrin (beta-CD) enhancing fluorescence. At D Delta lambda = 23 nm, 1-OHP and beta-NAP exhibit maximum signal with minimum interferences from alpha-NAP. At Delta lambda = 175 nm, the signal of alpha-NAP is not influenced by the presence of 1-OHP and beta-NAP. The signals detected at these three wavelengths, 360.2 nm, 330.6nm and 296.4nm, vary linearly when the concentration of 1-OHP, beta-NAP and alpha-NAP is in the range of 0.65-218.3 ng mL(-1), 2.8-1441.0 ng mL(-1) and 3.6-1586.0 ng mL(-1), respectively. The limits of detection (LOD) for alpha-NAP, beta-NAP and 1-OHP were 1.53 ng mL(-1), 0.78 ng mL(-1) and 0.020 ng mL(-1) with relative standard deviations (RSD) of 2.3%, 2.4% and 1.8%, respectively. The proposed method was successfully applied for the simultaneous determination of alpha-NAP, beta-NAP and 1-OHP in human urine samples, and the obtained results were in good agreement with those obtained by the method of high-performance liquid chromatography (HPLC).
语种：
英文
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常德地区学龄儿童碘营养水平调查与分析
作者：
薛金花;杨欢欢;谢燕湘
期刊：
临床合理用药杂志 ,2011年4(01X):10-12 ISSN：1674-3296
作者机构：
南华大学公共卫生学院,湖南省衡阳市,421001;421001,湖南省衡阳市,南华大学公共卫生学院;湖南省常德市疾病预防控制中心;[薛金花; 杨欢欢] 南华大学;[谢燕湘] 常德市疾病预防控制中心
关键词：
常德;儿童;学龄前;尿碘;盐碘
摘要：
目的 调查常德地区8～10岁儿童尿碘水平,了解该地区居民的碘营养状况.方法 通过人口容量比例概率抽样法(PPS)采集儿童尿样,同时采集食用碘盐,用分光光度法和滴定法分别测定尿样和盐样中碘含量,对测定结果进行统计分析.结果 8～10岁儿童尿碘中位数为364μg/L;经秩和检验,各个县尿碘水平之间差异有统计学意义(P＜0.05);男生与女生尿碘水平间差异有统计学意义(P＜0.05);其中澧县男、女生尿碘水平间差异有统计学意义(P＜0.05).居民户盐碘均值和合格率分别为23.5mg/kg、97.2%.结论 常德地区学龄儿童尿碘水平达到国家卫生部消除碘缺乏病的标准,学龄儿童碘营养状况良好,但仍存在地区和性别差异.
语种：
中文
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表面活性剂增敏荷移反应紫外分光光度法测定三聚氰胺
作者：
程健琳;吕昌银;冯伟;严成艳;薛金花;...
期刊：
分析试验室 ,2011年30(8):65-68 ISSN：1000-0720
作者机构：
[程健琳; 吕昌银; 冯伟; 严成艳; 薛金花; 蒋艺] 南华大学公共卫生学院
关键词：
三聚氰胺;荷移反应;四氯对苯醌;十六烷基三甲基溴化铵
摘要：
在pH=7.55的Tris-HC l缓冲溶液中,以十六烷基三甲基溴化铵（CTMAB）为增敏剂,三聚氰胺与四氯对苯醌之间发生荷移反应,研究了其反应产物的紫外光谱性质,据此建立了荷移反应紫外分光光度法测定三聚氰胺的方法。三聚氰胺的线性范围为8.61×10-8～2.60×10-6mol/L,r=0.9995,检出限为2.58×10-8mol/L,相对标准偏差为1.9%。在测定波长309nm处,配合物的表观摩尔吸光系数为7.50×105L.mol-1.cm-1。按国标法处理样品,采用本法测定,回收率偏低;利用自制分子印迹聚合物固相萃取柱对牛奶样品进行预处理后,检测结果满意。方法为奶制品中三聚氰胺的筛查和检测提供了新方法。
语种：
中文
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金纳米粒子共振光散射法测金属硫蛋白
作者：
薛金花;李巧玲;张锦泉;王永生
期刊：
环境与职业医学 ,2011年28(4):243-246 ISSN：2095-9982
作者机构：
南华大学公共卫生学院,湖南,衡阳,421001;[薛金花; 李巧玲; 张锦泉; 王永生] 南华大学
关键词：
金纳米粒子;金属硫蛋白;共振光散射
摘要：
[目的]建立并应用金纳米粒子共振光散射（RLS）法，测定人尿样中金属硫蛋白（MTs）的含量。[方法]在pH4．56的B-R缓冲液中，MTs与金纳米粒子结合形成较大聚集体，导致体系RLS增强，于545nm波长处RLS强度与MTs的浓度有线性关系，据此建立测定MTs的新方法。[结果]在最佳实验条件下，在545nm波长处RLS强度与MTs在7．98×10^-9-1．0×10^-7g／mL范围内线性关系良好，其线性回归方程为A／Rts=16．69p-23．09，相关系数r=0．9995，检出限为2．39×10^-9g／mL。[结论]本方法操作简便、快速、仪器设备简单，检出限低，灵敏度高，可用于尿中痕量MTs的测定。
语种：
中文
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卫生检验专业创新性实践教学模式的探讨
作者：
薛金花;王永生;杨慧仙;陈云生;于军晖;...
期刊：
人力资源管理 ,2010年(04):171,173 ISSN：1673-8209
作者机构：
南华大学公共卫生学院,湖南,衡阳,421001
关键词：
卫生检验专业;实践教学模式;创新性人才
摘要：
高校实践教学是培养创新性人才的重要环节,探索构建创新性实践教学模具有重要意义.本文讨论了传统教学模式的缺陷和不足,探讨构建"开放式,三环节,多层次,一体化"的实践教学新体系和创新性实践教学模式,为高校实践教学改革提供新思路和可供借鉴模式.
语种：
中文
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胶束增敏分光光度法水相测定乳制品中三聚氰胺
作者：
冯伟;吕昌银;严成艳;陈兴会;薛金花;...
期刊：
分析试验室 ,2010年29(4):49-52 ISSN：1000-0720
作者机构：
[冯伟; 吕昌银; 严成艳; 陈兴会; 薛金花; 程健琳] 南华大学公共卫生学院;大理学院公共卫生学院
关键词：
三聚氰胺;紫外-可见分光光度法;茜素红;胶束增敏;水相
摘要：
建立了一种基于胶束增敏缔合反应水相测定乳制品中三聚氰胺的分光光度法,研究了在阴离子表面活性剂十二烷基硫酸钠(SDS)形成的胶束体系中,茜素红(AR)阴离子与三聚氰胺阳离子反应产物的紫外-可见光谱特性.实验发现,加入三聚氰胺后,体系λmax由426 nm红移到516 nm,加入SDS对缔合物有显著的增稳、增敏效应.三聚氰胺质量浓度在1.9～25 mg/L范围内符合比尔定律,缔合物的表观摩尔吸光系数为6.9×10~3 L·mol~(-1)·cm~(-1).本方法用于液态奶及奶粉中三聚氰胺的测定,回收率分别为94.5%～104.8%和95.5%～100.4%,相对标准偏差分别为1.6%～2.2%和1.7%～2.6%.考察了酸效应、胶束对体系的作用机理,发现三聚氰胺与AR缔合物之间主要是通过静电引力和疏水作用力相结合.
语种：
中文
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Quartz crystal microbalance with β-cyclodextrin/TiO2 composite films coupled with chemometrics for the simultaneous determination of urinary 1- and 2-naphthol
作者：
Yuan, Yu-Kun;Xiao, Xi-Lin;Wang, Yong-Sheng* ;Xue, Jin-Hua;Li, Gui-Rong;...
期刊：
Sensors and Actuators B-Chemical ,2010年145(1):348-354 ISSN：0925-4005
通讯作者：
Wang, Yong-Sheng
作者机构：
[Yuan, Yu-Kun; Shi, Lin-Fei; Kang, Rong-Hui; Li, Gui-Rong; Zhang, Jin-Quan; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, W Changsheng Rd 28, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, W Changsheng Rd 28, Hengyang 421001, Peoples R China.
关键词：
Naphthol;beta-Cyclodextrin;TiO2;Quartz crystal microbalance;Chemometrics
摘要：
A novel sensitive method was presented for the simultaneous determination of 1-naphthol (1-NAP) and 2-naphthol (2-NAP) in human urine samples. This method is based on the combination of the high sensitivity of quartz crystal microbalance (QCM) and excellent predictive ability of chemometrics. Nanocrystalline TiO<inf>2</inf> films were used as substrates for immobilizing β-cyclodextrin (β-CD) onto the QCM surface. The sensing layer consisting of β-CD and nanocrystalline TiO<inf>2</inf> particles was characterized by X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TG) and IR spectroscopy. In the Britton-Robinson (B-R) buffer solution of pH 6.37, frequency shift (ΔF) of the QCM was directly proportional to the concentration of 1-NAP and 2-NAP as 1.0 ×10<sup>-6</sup> to 1.3 ×10<sup>-4</sup> mol L<sup>-1</sup>, and 1.0 ×10<sup>-6</sup> to 1.2 ×10<sup>-4</sup> mol L<sup>-1</sup>, respectively. The correlation coefficients were 0.993 for 1-NAP, and 0.990 for 2-NAP. The limits of detection (LOD) were found to be 8.362 ×10<sup>-7</sup> mol L<sup>-1</sup> for 1-NAP and 2.146 ×10<sup>-7</sup> mol L<sup>-1</sup> for 2-NAP. The proposed method has been successfully applied for detecting 1-NAP and 2-NAP in human urine with a satisfactory result. The QCM with β-CD/TiO<inf>2</inf> composite films coupled with chemometrics can lead to significant improvements in the sensor performance, and would be benefit to extend the scope of application of QCM and chemometrics. ©2009 Elsevier B.V. All rights reserved.
语种：
英文
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基体改进剂石墨炉原子吸收测定发镉的研究
作者：
向艳;薛金花;马会会
期刊：
应用化工 ,2010年39(10):1590-1593 ISSN：1671-3206
作者机构：
1. 南华大学公共卫生学院;2. 连云港市疾病预防控制中心
关键词：
石墨炉原子吸收;基体改进剂;镉;加标回收率;基体干扰
摘要：
探讨石墨炉原子吸收检测发镉的基体干扰,选择适宜的基体改进剂建立一种测定发镉的新方法。样品经前处理后,分别加入单种基体改进剂和不同组合基体改进剂,用石墨炉原子吸收测定样品及加标样品,通过加标回收率筛选出测定发镉的优良基体改进剂。研究得出测定发镉的最佳基体改进剂为硝酸铵-抗坏血酸-硝酸镧混合基体改进剂。在0.219 4~11.236 1 ng/mL的范围内,镉原子吸光强度与其含量有良好的线性关系,标准曲线y=0.060＋0.017 3ρ（ng/mL）,r=0.999 8,相对标准偏差为2.9%~5.7%,方法的检出限为0.131 7 ng/mL,平均回收率94.5%。该方法简便易行、灵敏度高、选择性好、准确度高,测定结果令人满意。
语种：
中文
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Rapid simultaneous analysis of 1-hydroxypyrene, 2-hydroxyfluorene, 9-hydroxyphenanthrene, 1- and 2-naphthol in urine by first derivative synchronous fluorescence spectrometry using Tween-20 as a sensitizer
作者：
Kang, Rong-Hui;Wang, Yong-Sheng* ;Yang, Hong-Mei;Li, Gui-Rong;Tan, Man;...
期刊：
Analytica Chimica Acta ,2010年658(2):180-186 ISSN：0003-2670
通讯作者：
Wang, Yong-Sheng
作者机构：
[Yuan, Yu-Kun; Tan, Man; Shi, Lin-Fei; Kang, Rong-Hui; Li, Gui-Rong; Zhang, Jin-Quan; Yang, Hong-Mei; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Derivative synchronous fluorescence;Polycyclic aromatic hydrocarbons;Tween-20;Urine
摘要：
A novel method of first derivative synchronous fluorescence was developed for the rapid simultaneous analysis of trace 1-hydroxypyrene (1-OHP), 1-naphthol (1-NAP), 2-naphthol (2-NAP), 9-hydroxyphenanthrene (9-OHPe) and 2-hydroxyfluorene (2-OHFlu) in human urine. Only one single scan was needed for quantitative determination of five compounds simultaneously when Δλ = 10 nm was chosen. In the optimal experimental conditions, there was a linear relationship between the fluorescence intensity and the concentration of 1-OHP, 1-NAP, 2-NAP, 9-OHPe and 2-OHFlu in the range of 1.75 × 10-9 to 4.50 × 10-6 mol L-1, 3.64 × 10-8 to 2.20 × 10-4 mol L-1, 8.18 × 10-9 to 1.20 × 10-4 mol L-1, 3.26 × 10-9 to 8.50 × 10-5 mol L-1 and 4.88 × 10-9 to 5.50 × 10-6 mol L-1, respectively. The limits of detection (LOD) were found to be 5.25 × 10-10 mol L-1 for 1-OHP, 1.10 × 10-8 mol L-1 for 1-NAP, 2.46 × 10-9 mol L-1 for 2-NAP, 9.77 × 10-10 mol L-1 for 9-OHPe and 1.46 × 10-9 mol L-1 for 2-OHFlu. The proposed method is reliable, selective and sensitive, and has been used successfully in the determination of traces of 1-OHP, 1-NAP, 2-NAP, 9-OHPe and 2-OHFlu in human urine samples, whose results were in good agreement with those gained by the HPLC method. © 2009 Elsevier B.V. All rights reserved.
语种：
英文
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LGSF-MTs体系的共振散射光谱及分析应用
作者：
薛金花;王永生;何爱桃;郝玉娥
期刊：
中南医学科学杂志 ,2010年38(1):31-33 ISSN：2095-1116
作者机构：
南华大学,公共卫生学院,湖南,衡阳,421001;[薛金花; 何爱桃; 郝玉娥; 王永生] 南华大学
关键词：
亮绿SF淡黄;金属硫蛋白;生物标志物;共振光散射
摘要：
目的 建立LGSF-RLS增强定量检测尿样中金属硫蛋白(MTs)分析的新方法 . 方法 在pH2.5 BR缓冲溶液中,亮绿SF淡黄(LGSF)与MTs作用,导致体系的共振光散射(RLS)明显增强. 结果 LGSF-MTs体系在468 nm波长处的RLS增强值(ΔI)与MTs的浓度(ρMTs)有良好的线性关系,线性范围0.05~5.00 μg/mL,相关系数为0.9992,方法 检出限15.50 ng/mL,相对标准偏差1.01%～5.15%(n=9),平均加标回收率94.7%. 结论 该方法 最突出的特点是灵敏、准确、易于推广,用于样品测定结果较准确.
语种：
中文
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衡阳市食用菌中铅、镉、汞调查评价
作者：
向艳;薛金花;袁良华
期刊：
山东化工 ,2010年39(10):24-27,31 ISSN：1008-021X
作者机构：
南华大学,公共卫生学院,湖南,衡阳,421001;[薛金花; 袁良华; 向艳] 南华大学
关键词：
食用菌;铅;镉;汞;调查评价
摘要：
检测衡阳市食用菌中重金属铅、镉、汞的含量，探讨重金属污染来源，提出相应的预防和干预措施。通过随机采样的方法采集鲜、千食用菌，利用石墨炉原子吸收光谱法和冷原子荧光法，分别检测食用菌中铅、镉与汞的含量。80份食用菌中，铅未见超标，其中含量最高的为干松乳菇达1．0222mg／kg；镉总超标率为15％，其中含量最高的为干香菇达0．2642mg／kg；汞也未见超标，其含量最高的为鲜香菇达0．0251mg,／kg。本研究表明衡阳市市场上销售的食用菌存在重金属污染的风险不大。不同类型的食用菌样品以干、鲜香菇、干松乳菇镉超标较多；不同重金属类型以镉污染的风险相对较大，铅、汞污染的风险较小。
语种：
中文
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静态顶空-毛细管柱气相色谱法检测水中甲基叔丁基醚
作者：
眭世闺;薛金花;何爱桃;贺性鹏
期刊：
中国卫生检验杂志 ,2010年20(3):525-526+540 ISSN：1004-8685
作者机构：
南华大学公共卫生学院劳动卫生与环境卫生学教研室,湖南衡阳,421001
关键词：
甲基叔丁基醚;静态顶空;毛细管柱;气相色谱法检测;加标回收率;检测器温度;分流比;色谱柱温;峰面积;检出限
摘要：
目的:建立适用于水中甲基叔丁基醚的静态顶空气相色谱测定方法.方法:采用静态顶空极性毛细管柱气相色谱法测定水中甲基叔丁基醚.结果:在所建立的实验条件下水中甲基叔丁基醚的检出限为0.0163μg/L,线性范围为0.0156～120 mg/L,其相关系数r=0.9997,样品的加标回收率为92.6%～100.1%,相对标准偏差(RSD)低于1.1%.结论:此方法能够用于分析水中甲基叔丁基醚.
语种：
中文
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巯基棉富集分离二甲酚橙光度法测定水中痕量镉
作者：
薛金花;康荣慧;李程;向艳;王永生
期刊：
中国工业医学杂志 ,2009年22(3):226-228 ISSN：1002-221X
作者机构：
南华大学公共卫生学院,湖南,衡阳,421001
关键词：
镉;二甲酚橙;分光光度法;原子吸收分光光度法
摘要：
在pH6.3 KH2PO42-Na2B4O7缓冲溶液中,镉与二甲酚橙发生反应生成紫红色配合物,在578 nm处吸光度值明显增强,据此建立测定镉的新方法.方法线性范围0.020～6.0μg/ml,线性回归方程Y=0.063 2 P(μg/mal)+0.119 8,相关系数0.9982,加标回收率在95.0%～106.0%.该方法检出范围宽、选择性好、简便快速,与原子吸收分光光度法的测定结果基本一致.
语种：
中文
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Pb-XO配合物显色光度法测定水样中微量铅
作者：
肖锡林;魏永卷;薛金花;袁玉坤;王永生
期刊：
应用化工 ,2009年38(2):296-299 ISSN：1671-3206
作者机构：
[肖锡林; 魏永卷; 薛金花; 袁玉坤; 王永生] 南华大学,公共卫生学院化学化工学院
关键词：
分光光度法;二甲酚橙;铅
摘要：
二甲酚橙是微量分析中一种很重要的光度显色剂,在pH 6.0的磷酸-醋酸-硼酸缓冲溶液中,二甲酚橙与铅形成稳定的1∶1红色配合物,导致吸光度的增强.研究了铅与三苯甲烷类染料二甲酚橙的显色反应体系,发现在波长580 nm处吸光度的增强值与铅的加入量有很好的线性关系,据此建立了测定微量铅的新方法.该方法的摩尔吸光数为3.312×10~4 L/(mol · cm),铅离子的浓度在0.075～1.2 mg/L范围内符合比耳定律.方法简便、灵敏、选择性好,可广泛应用.
语种：
中文
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MTs-Hg(Ⅱ)-KI-PVA-XO体系的共振光散射光谱及其分析应用
作者：
薛金花;王永生;向艳
期刊：
应用化工 ,2009年38(5):744-747,751 ISSN：1671-3206
作者机构：
[薛金花; 向艳] 南华大学公共卫生学院;[王永生] 南华大学,公共卫生学院
关键词：
生物标志物;金属硫蛋白;共振光散射;二甲酚橙;汞
摘要：
建立检测MTs的新方法.在pH 2.5的缓冲溶液中,MTs-Hg(Ⅱ)-KI-PVA-XO体系在505 nm处共振光散射增强最大,且增强的强度与MTs的加入量呈线性关系,据此检测MTs.结果表明,检出限21.58 ng/mL,线性范围0.072～6.3 μg/mL,相关系数0.994 9,相对标准偏差3.67%～5.61%, 平均回收率95.6% (n=6).该方法用于测定尿样MTs结果满意.
语种：
中文
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