期刊论文

Determination of Metallothioneins by Resonance Light Scattering Method with Gold Nanoparticles

中文

环境与职业医学

2095-9982

2011

28

4

243-246

10.13213/j.cnki.jeom.2011.04.022

湖南省自然科学基金资助项目（编号：03JJY3030） 衡阳市基础研究计划项目（编号：2009KJ01）

本校为第一机构

[目的]建立并应用金纳米粒子共振光散射（RLS）法，测定人尿样中金属硫蛋白（MTs）的含量。[方法]在pH4．56的B-R缓冲液中，MTs与金纳米粒子结合形成较大聚集体，导致体系RLS增强，于545nm波长处RLS强度与MTs的浓度有线性关系，据此建立测定MTs的新方法。[结果]在最佳实验条件下，在545nm波长处RLS强度与MTs在7．98×10^-9-1．0×10^-7g／mL范围内线性关系良好，其线性回归方程为A／Rts=16．69p-23．09，相关系数r=0．9995，检出限为2．39×10^-9g／mL。[结论]本方法操作简便、快速、仪器设备简单，检出限低，灵敏度高，可用于尿中痕量MTs的测定。

To establish a resonance light scattering（ RLS ）method for the determination of metallothioneins （ MTs ） based on the interaction of MTs with gold nanoparticles （ GNPs ）. [ Methods ] In B-R buffer solution of pH 4.56, the conjugates of MTs-GNPs were formed by Au-S covalent bond between the mereapto group of eysteine of MTs and GNPs, resulting in the enhancement of RLS intensity of the system owing to their aggregating. RLS intensity linearly correlated with the concentration of MTs at 2m~x=545nm, which suggested that a new method for dete...