期刊论文

Wang, Yong-Sheng

英文

Analytical Letters

0003-2719

2012

45

18

2774-2784

10.1080/00032719.2012.703738

Received 1 April 2012; accepted 10 June 2012. The authors gratefully acknowledge the support of the National Natural Science Foundation of China (No. 30572191), the Science and Technology Programs of Hunan Province in China (No. 2010SK3039, 2008FJ3083), and the Science Research Program of Educational Department of Hunan Province in China (No. 08C718). Address correspondence to Yong-Sheng Wang, College of Public Health, University of South China, Hengyang 421001, P.R. China. E-mail: yongsheng.w@tom.com

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A novel method for the determination of semicarbazide-sensitive amine oxidase (SSAO) activity in blood plasma has been developed. The method was based on the change of light scattering (LS) intensity resulting from the derivative product of the interaction of 2,4-dinitrophenylhydrazine (DNPH) with benzaldehyde produced by catalyzing of SSAO to benzylamine. In Britton-Robinson (B-R) buffer solution, the intensity of system's LS at 514.6 nm was significantly enhanced and was directly proportional to the concentration of benzaldehyde. In this meth...