期刊论文

中文

环境与健康杂志

1001-5914

2014

31

6

525-527

10.16241/j.cnki.1001-5914.2014.06.006

国家自然科学基金（31200140）；

本校为第一机构

目的建立水中痕量镉(Ⅱ)的荧光素-曙红Y能量转移荧光猝灭测定法。方法取0.50ml的水样置于5.0 ml的比色管中,分别加入1×10~(-4) mol/L荧光素溶液0.30 ml,1×10~(-5) mol/L曙红Y溶液0.10 ml,BR缓冲液(pH 6.5)0.50 ml,定容后在502 nm处测定溶液荧光强度(F)值,以内标法定量。结果在3.43×10~(-7) ~1.246×10~(-5) mol/L的线性范围为,该方法所得镉(Ⅱ)的线性回归方程为ΔF=14.221+546.81×10~(-5)c,r=0.999 5。该方法的检出限为1.03×10~(-8) mol/L,平均回收率为94.53%~98.15%,RSD为2.44%~4.67%。结论该方法操作简便、灵敏、选择性好,适用于水中镉(Ⅱ)的测定。

Objective To develop a novel energy transfer fluorescence quenching method for the determination of cadmium using fluorescein-eosine Y. Methods A total of 0.50 ml water sample, 1×10^-4 mol/L fluorescein solution 0.30 ml, 1 ×10^-5 mol/L eosin Y solution 0.10 ml and BR buffer （pH 6.5） 0.50 ml were added into 5 ml eolorimetric tube, then the fluorescence intensity was determined at 502 nm after constant volume. Results Under the optimal experimental conditions,there was a linear relationship between fluorescence intensity and the concentration...