期刊论文

Wang, Yong-Song

英文

Biosensors and Bioelectronics

0956-5663

2014

58

22-26

10.1016/j.bios.2014.02.047

The authors gratefully acknowledge the support of the National Natural Science Foundation of China (No. 21177052 ), the Foundation of National 863 Plan of China (No. 2012AA063501 ), the Science and Technology Program of Hunan Province in China (No. 2010SK3039 ) and the Construct Program of the Key Discipline (Public Health and Preventive Medicine) in Hunan Province .

本校为第一且通讯机构

We developed a highly sensitive label-free assay of 8-hydroxy-2'-deoxyguanosine (8-OHdG) using 8-OHdG-aptamer (Apt) as the recognition element. The Apt was adsorbed onto the surface of gold nanoparticles (AuNPs), which prevents them from aggregating under high-salt conditions. Upon addition of 8-OHdG, the conformation of the Apt changes to form a G-quadruplex structure, which leads to the aggregation of the AuNPs along with the increase of the resonance light scattering intensity. The mechanism of 8-OHdG that induces Apt to form G-quadruplexes ...