期刊论文

中文

应用化工

1671-3206

2012

41

3

507-509

10.16581/j.cnki.issn1671-3206.2012.03.016

(2010SK3039):湖南省科技厅科技计划资助项目

本校为第一机构

在pH=6的BR缓冲溶液中,环丙沙星-铜配合物与金属硫蛋白作用,导致体系吸光强度增强,在272 nm波长处,吸光强度与金属硫蛋白的浓度呈线性关系,据此建立了测定金属硫蛋白的新方法。方法的线性范围为0.259～1.54μmol/L,检出限7.76×10-8mol/L,样品平均加标回收率为91.8%。本方法操作简便、快速,灵敏,可用于实际样本测定,结果满意。

In the BR buffer solution（pH=6）,CIP-Cu chelate interacted with MTs to lead to enhance the absorbance intensity of system,which was proportional to the concentration of MTs at 272 nm.Based on this phenomenon,a new method for the determination of MTs was proposed.The linear range for MTs was 0.259~1.54 μmol/L with a determination limit of 7.76×10-8 mol/L.The average recovery was 91.8%.The method is simple,rapid,sensitive,and had been applied to det...