期刊论文

Wang, Yong-Sheng

英文

Analytical Biochemistry

0003-2697

2014

458

4-10

10.1016/j.ab.2014.04.031

The sensing principle for the colorimetric aptasensor is based on the idea that binding of Apt with 8-OHdG results in the conformational change of Apt from a random coil structure to a G-quadruplex structure, which interacts with hemin to form a peroxidase-like DNAzyme. The resulting G-quadruplex DNAzyme could catalyze the oxidation of ABTS2− by H2O2 to ABTS+, concomitantly generating a color change of the solution. Therefore, the strategy for designing an aptasensor can be summarized as how to

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A highly sensitive, low-cost colorimetric aptasensor was developed for the determination of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in human urine. The method is based on a conformational switching of the 8-OHdG aptamer to form a G-quadruplex structure in the presence of 8-OHdG. The resulting G-quadruplex assembles into a peroxidase-like DNAzyme with hemin, which effectively catalyzes the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS2-) by H2O2 to ABTS +, resulting in an increase in the absorption sign...