A novel method of resonance light scattering (RLS) was developed for the analysis of trace metallothioneins (MTs) in human urine. In a CH 3COOH-CH3COONa buffer solution of pH 4.5, the formation of a complex between levofloxacin (LEV)-Pd and MTs led to enhance the RLS intensity of the system, and the enhanced RLS intensity at 468 nm was proportional to the concentration of MTs in the range of 0.059-22.4 μg mL-1. The linear regression equation was ΔI = 127.5 ρ(μg mL-1)-88.02 with a correlation coefficient of 0.9992, and the detection limit of 17.8 ng mL-1. The relative standard d...