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Resonance light scattering determination of metallothioneins using levofloxacin−palladium complex as a light scattering probe

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成果类型:
期刊论文
作者:
Xue, Jin-Hua;Qian, Qiu-Mei;Wang, Yong-Sheng*;Meng, Xia-Ling;Liu, Lu
通讯作者:
Wang, Yong-Sheng
作者机构:
[Qian, Qiu-Mei; Xue, Jin-Hua; Wang, Yong-Sheng; Liu, Lu; Meng, Xia-Ling] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构:
[Wang, Yong-Sheng] U
Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
语种:
英文
关键词:
Levofloxacin hydrochloride;Metallothioneins;Palladium;Resonance light scattering
期刊:
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
ISSN:
1386-1425
年:
2013
卷:
102
页码:
205-211
基金类别:
Natural Science Foundation of Hunan Province in ChinaNatural Science Foundation of Hunan Province [03JJY3030]; Science and Technology Program of Hunan Province in China [2010SK3039]; Science Research Program of Educational Department of Hunan Province in China [08C718]
机构署名:
本校为第一且通讯机构
院系归属:
公共卫生学院
摘要:
A novel method of resonance light scattering (RLS) was developed for the analysis of trace metallothioneins (MTs) in human urine. In a CH 3COOH-CH3COONa buffer solution of pH 4.5, the formation of a complex between levofloxacin (LEV)-Pd and MTs led to enhance the RLS intensity of the system, and the enhanced RLS intensity at 468 nm was proportional to the concentration of MTs in the range of 0.059-22.4 μg mL-1. The linear regression equation was ΔI = 127.5 ρ(μg mL-1)-88.02 with a correlation coefficient of 0.9992, and the detection limit of 17.8 ng mL-1. The relative standard d...

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