摘要：
The production of hydrogen peroxide (H 2 O 2 ) through photosynthesis represents a highly efficient and environmentally sustainable approach. Nonetheless, the scalability of photocatalytic H 2 O 2 production remains constrained by factors such as slow reaction kinetics and the rapid recombination of charge carriers. This study elaborates on the rational design of a uniform S-scheme SCN/V S -SnS 2 , which effectively minimizes the charge transfer distance while simultaneously enhancing the electric field at the interface. The charge transfer by the S-scheme pathway was confirmed by in situ irradiated XPS spectroscopy and ultraviolet photoelectron spectroscopy (UPS). The improvement effectively optimizes electron transfer pathways via an S-scheme mechanism, showcasing a promising advancement in charge transport efficiency. The optimized SCN/V S -SnS 2 demonstrates an H 2 O 2 evolution rate of 232.4 μ mol g -1 h −1 with pure water at a pH of 7, which surpasses that of most CN-based materials. Experimental and theoretical results reveal that S vacancies in V S -SnS 2 facilitates hole-water oxidation reaction (WOR), whereas S doping site in SCN promotes oxygen activation during oxygen reduction reaction (ORR), ultimately lowering the energy barrier for H 2 O 2 evolution. The present interface regulation strategy presents a novel perspective on the design of efficient 2D/2D S-scheme photocatalysts to produce H 2 O 2 .
The production of hydrogen peroxide (H 2 O 2 ) through photosynthesis represents a highly efficient and environmentally sustainable approach. Nonetheless, the scalability of photocatalytic H 2 O 2 production remains constrained by factors such as slow reaction kinetics and the rapid recombination of charge carriers. This study elaborates on the rational design of a uniform S-scheme SCN/V S -SnS 2 , which effectively minimizes the charge transfer distance while simultaneously enhancing the electric field at the interface. The charge transfer by the S-scheme pathway was confirmed by in situ irradiated XPS spectroscopy and ultraviolet photoelectron spectroscopy (UPS). The improvement effectively optimizes electron transfer pathways via an S-scheme mechanism, showcasing a promising advancement in charge transport efficiency. The optimized SCN/V S -SnS 2 demonstrates an H 2 O 2 evolution rate of 232.4 μ mol g -1 h −1 with pure water at a pH of 7, which surpasses that of most CN-based materials. Experimental and theoretical results reveal that S vacancies in V S -SnS 2 facilitates hole-water oxidation reaction (WOR), whereas S doping site in SCN promotes oxygen activation during oxygen reduction reaction (ORR), ultimately lowering the energy barrier for H 2 O 2 evolution. The present interface regulation strategy presents a novel perspective on the design of efficient 2D/2D S-scheme photocatalysts to produce H 2 O 2 .
摘要：
A method for the facile synthesis of valuable 3‐selenospiro[4,5]decatrienones via CuBr2‐catalyzed three‐component reaction of N‐(4‐methoxyaryl)‐N‐methyl‐3‐phenylpropiolamide, Se powder, and boronic acids under air is disclosed. This method feature wide substrate scope, good functional group tolerance, employing earth‐abundant metal as the catalyst and green air as the oxidant, which increase its practicability. Abstract Herein, a method for the assembly of biologically valuable 3‐selenospiro[4,5]decatrienones through CuBr2‐catalyzed ispo‐cyclization of Se powder, boronic acids, and N‐(p‐methoxyaryl)propiolamides has been established. In this protocol, noble transition metal, prefunctionalized selenylation reagent, and strong chemical oxidant are not employed. This method feature wide substrate scope, good functional group tolerance, easy operation, and employing earth‐abundant metal as catalyst and green air as oxidant. Furthermore, several derivatizations of 3‐selenospiro[4,5]decatrienones are performed to showcase the practicability of our strategy.
摘要：
Abstract Herein, we demonstrate the synthesis of azafluoranthene derivatives under mild conditions via [2+2+2] cycloaddition of 1,6‐diynes and nitriles catalyzed by CoBr2/dppp, exhibiting good atom economy. This procedure uses an inexpensive ligand and an earth‐abundant metal as catalyst to lower the cost of synthesis. Several azafluoranthenes are produced in 28–95% yields using this approach, featuring mild conditions, wide substrate scope, and good functional group tolerance. The fluorescence spectra of some azafluoranthenes indicate that these scaffolds have good photophysical properties for material science.
通讯机构：
[Cai, JH ] U;[Zhao, F ] H;[Wang, DH ] S;Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;Hunan Univ Med, Sch Pharmaceut Sci, Hunan Prov Key Lab Synthet Biol Tradit Chinese Med, Huaihua 418000, Hunan, Peoples R China.
摘要：
Herein, we disclosed a highly efficient pathway toward 3-selenylated chromone derivatives via electrocatalytic cascade selenylation/cyclization/deamination of 2-hydroxyaryl enaminones with diselenides. This method showed mild conditions, easy operation, wide substrate scope, and good functional group tolerance. Furthermore, this electrosynthesis strategy was amendable to scale-up the reaction. Additionally, the preliminary experiments revealed that this reaction probably proceeded via a cation pathway instead of a radical pathway.
期刊：
CURRENT PHARMACEUTICAL BIOTECHNOLOGY,2024年25(12):1814-1824 ISSN：1389-2010
通讯作者：
Yang, XY
作者机构：
[Yang, Xiaoyan; Yang, XY; Xie, Haimei; Lei, Xiaoyong; Yu, Jia] Univ South China, Hengyang Med Coll, Sch Pharmaceut Sci, 28 Western Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.;[Ou, Zhiwen] Univ South China, Chuanshan Coll, 28 Western Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.;[Yang, Xiaoyan; Lei, Xiaoyong] Univ South China, Hunan Prov Key Lab Tumor Microenvironm Respons Dru, 28 Western Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Yang, XY ] U;Univ South China, Hengyang Med Coll, Sch Pharmaceut Sci, 28 Western Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
PKM2;cancer;circRNA.;lncRNA;miRNA;ncRNAs
摘要：
Cancer is one of the main reasons for death, and it threatens human life and health. Both the environment and genes can lead to cancers. It dates back more than a million years; more importantly, tumor cells can not be detected until they grow to a large number. Currently, cancers are treated with surgical excision or non-surgical procedures. By studying the interaction between ncRNAs and PKM2, we aim to provide new targets for diagnosis, treatment, and prognosis for cancers. Read relevant articles and made a summary and classification. Non-coding RNAs (ncRNAs) are RNAs that do not code for proteins. They perform a function in transcription and translation and can be used as targets for cancer therapy. Pyruvate kinase M2 (PKM2) is a form of PKM, and it catalyzes the glycolysis of the final cellular processes to promote tumorigenesis. Not only that, but it also plays non-metabolic functions, including the expression of the gene, cell proliferation, cell migration, and tumor angiogenesis in cancer cells. The existing studies have found that microRNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) can promote or inhibit the aerobic glycolysis of cancer cells by affecting PKM2, which increases or decrease the risk of cancers and affect the progression of cancers. This review focuses on the mechanism of ncRNAs regulating PKM2 in cancers and summarizes the roles of some ncRNAs.
摘要：
As lncRNAs have increasingly been investigated, they are no longer simply defined as RNAs with no transcription capability. Studies have identified significant associations between the abnormal expression of lncRNAs and human diseases, particularly the mechanisms by which lncRNAs play a part in cancers, which are of considerable attention to researchers. As a result of the complex spatial structure, the mechanisms of interaction of lncRNAs in cancer cells are also complicated and diversified. Among a series of lncRNAs, TUG1, which is now considered to be a very high-value lncRNA, has recently been identified to express abnormally in some malignancies, leading to different alterations in cancer cells proliferation, migration, invasion, apoptosis, and drug resistance, and hence promoting or inhibiting cancer progression. Current studies have implicitly indicated that TUG1 can be used as a therapeutic target for human cancers. However, the biological functions of TUG1 have been studied for a short period of time, and the complete molecular mechanism still needs to be clarified. Accordingly, this review focuses on the principal molecular mechanisms of TUG1 in human cancers and the specific mechanisms of action in different cancer development processes based on existing studies.
摘要：
<jats:title>Abstract</jats:title><jats:p>A copper‐catalyzed direct C(<jats:italic>sp</jats:italic><jats:sup><jats:italic>2</jats:italic></jats:sup>)−H bond aminosulfonylation of maleimides is demonstrated. This protocol enables concurrent construction of C(<jats:italic>sp</jats:italic><jats:sup><jats:italic>2</jats:italic></jats:sup>)−N and C(<jats:italic>sp</jats:italic><jats:sup><jats:italic>2</jats:italic></jats:sup>)−SO<jats:sub>2</jats:sub> bonds in one‐step and features high efficiency, broad substrate scopes, good functional‐group tolerance, and mild reaction conditions. Preliminary mechanistic studies indicate that the reaction probably involves a radical way. Significantly, this method is applicable to synthesize derivatives of pharmaceuticals such as Desloratadine, Fluoxetine, Atomoxetine, and Maprotiline.</jats:p>
摘要：
PIWI proteins have a strong correlation with PIWI-interacting RNAs (piRNAs), which are significant in development and reproduction of organisms. Recently, emerging evidences have indicated that apart from the reproductive function, PIWI/piRNAs with abnormal expression, also involve greatly in varieties of human cancers. Moreover, human PIWI proteins are usually expressed only in germ cells and hardly in somatic cells, so the abnormal expression of PIWI proteins in different types of cancer offer a promising opportunity for precision medicine. In this review, we discussed current researches about the biogenesis of piRNA, its epigenetic regulatory mechanisms in human cancers, such as N6-methyladenosine (m6A) methylation, histone modifications, DNA methylation and RNA interference, providing novel insights into the markers for clinical diagnosis, treatment and prognosis in human cancers.
摘要：
Sirtuins are a family of NAD + -dependent deacetylases that regulate some important biological processes, including lipid metabolism and autophagy, through their deacetylase function. Autophagy is a new discovery in the field of lipid metabolism, which may provide a new idea for the regulation of lipid metabolism. There are many tandem parts in the regulation process of lipid metabolism and autophagy of sirtuins protein family. This paper summarized these tandem parts and proposed the possibility of sirtuins regulating lipid autophagy, as well as the interaction and synergy between sirtuins protein family. Currently, some natural drugs have been reported to affect metabolism by regulating sirtuins, some of which regulate autophagy by targeting sirtuins.
Lipid metabolism disorders, such as diabetes and atherosclerosis, have seriously harmed human health and caused economic and social burden. The role of sirtuins protein family in aging has been widely concerned, but they also show value in the regulation of lipid metabolism. Autophagy is a new discovery in the field of lipid metabolism, which may provide a new idea for the regulation of lipid metabolism. There are many tandem parts in the regulation process of lipid metabolism and autophagy of sirtuins protein family. This paper summarized these tandem parts and proposed the possibility of sirtuins regulating lipid autophagy, as well as the interaction and synergy between sirtuins protein family.
摘要：
An electrochemical synthesis of various 3‐selenylated chromones via selenylation of alkynyl aryl ketones without the employment of catalyst and chemical‐oxidant is described here. This method features mild conditions, high efficiency, and wide substrate scope. Furthermore, the scale‐up reaction runs smoothly which indicates the practicability of this electrosynthesis strategy.
An electrochemical synthesis of various 3‐selenylated chromones via selenylation of alkynyl aryl ketones without the employment of catalyst and chemical‐oxidant is described here. This method features mild conditions, high efficiency, and wide substrate scope. Furthermore, the scale‐up reaction runs smoothly which indicates the practicability of this electrosynthesis strategy.
摘要：
Atherosclerosis (AS) is the pathophysiologic basis of many cardiovascular diseases. A number of studies have shown that post-translational modification (PTM) contributes to the initiation and progression of AS. For example, recent studies found that SUMOylation, ie, small ubiquitin-like modifier (SUMO) conjugation to target substrate proteins, was involved in AS. This PTM appears related to endothelial cell dysfunction (ECD), dyslipidemia and vascular smooth muscle cell (VSMC) proliferation. This review focuses on the molecular effects of SUMOylation in the initiation and progression of AS, including ECD, dyslipidemia and VSMC proliferation to better understand this pathologic process.
期刊：
Biochemical and Biophysical Research Communications,2019年516(1):278-284 ISSN：0006-291X
通讯作者：
Zhou, Rongrong
作者机构：
[Cheng, Tingting] Cent S Univ, Xiangya Hosp, Dept Prevent Hlth Care, Changsha, Hunan, Peoples R China.;[Zhang, Jing; Zhou, Rongrong; Liang, Zhan; Liu, Zhiyuan; Zhang, Zijian; Chen, Taili; Li, Jiahui; Tan, Zhaohua; Cheng, Tingting; Tang, Jianbing] Cent S Univ, Xiangya Hosp, Dept Oncol, Changsha, Hunan, Peoples R China.;[Zhang, Jing; Zhou, Rongrong; Liang, Zhan; Liu, Zhiyuan; Zhang, Zijian; Chen, Taili; Li, Jiahui; Tan, Zhaohua; Tang, Jianbing] Cent S Univ, Xiangya Hosp, Natl Clin Res Ctr Geriatr Disorders, Changsha, Hunan, Peoples R China.;[Cheng, Yuyu] Univ South China, Chuanshan Coll, Hengyang, Peoples R China.;[Zhao, Jin] Cent S Univ, Key Lab Carcinogenesis, Chinese Minist Hlth, Hunan Canc Hosp, Changsha, Hunan, Peoples R China.
通讯机构：
[Zhou, Rongrong] C;Cent S Univ, Xiangya Hosp, Dept Oncol, Natl Clin Res Ctr Geriatr Disorders, Changsha 410078, Hunan, Peoples R China.
关键词：
ETV4;Lung adenocarcinoma;MSI2;Proliferation and invasion;Transcription regulation
摘要：
The oncogenic roles of ETV4 have been revealed in multiple cancers. However, its expression and functions in lung cancer are rarely explored. Here, we firstly detected the expression of ETV4 in lung adenocarcinoma (LUAD) via online data and local experiment validation. Furthermore, we explored the functions and corresponding mechanisms of ETV4 in LUAD. Upregulation of ETV4 in LUAD is indicated by online data and our results of qPCR, Western blot and immunohistochemistry in collective tissue samples. ETV4 knockdown significantly inhibits proliferation and invasion in LUAD indicated by the outcomes of CCK8, plate clone formation, and Transwell invasion assays. Mechanistically, chromatin immunoprecipitation and luciferase reporter system assays indicated that ETV4 could directly bind at the promoter of MSI2 and promote its transcription. Furthermore, ectopic expression MSI2 can rescue the inhibitory effects caused by ETV4 knockdown in LUAD. Therefore, we proved that upregulation of ETV4 could promote proliferation and invasion of LUAD by transcriptionally upregulating MSI2 offering a potential therapy treatment target of LUAD. (C) 2019 Elsevier Inc. All rights reserved.
摘要：
Cardiovascular disease is a growing major global public health problem. Oxidative stress is regarded as one of the key regulators of pathological physiology, which eventually leads to cardiovascular disease. However, mechanisms by which FGF-2 rescues cells from oxidative stress damage in cardiovascular disease is not fully elucidated. Herein this study was designed to investigate the protective effects of FGF-2 in H2O2-induced apoptosis of H9c2 cardiomyocytes, as well as the possible signaling pathway involved. Apoptosis of H9c2 cardiomyocytes was induced by H2O2 and assessed using methyl thiazolyl tetrazolium assay, Hoechst, and TUNEL staining. Cells were pretreated with PI3K/Akt inhibitor LY294002 to investigate the possible PI3K/Akt pathways involved in the protection of FGF-2. The levels of p-Akt, p-FoxO3a, and Bim were detected by immunoblotting. Stimulation with H2O2 decreased the phosphorylation of Akt and FoxO3a, and induced nuclear localization of FoxO3a and apoptosis of H9c2 cells. These effects of H2O2 were abrogated by pretreatment with FGF-2. Furthermore, the protective effects of FGF-2 were abolished by PI3K/Akt inhibitor LY294002. In conclusion, our data suggest that FGF-2 protects against H2O2-induced apoptosis of H9c2 cardiomyocytes via activation of the PI3K/Akt/FoxO3a pathway.
摘要：
Objective Chronic stress is an important risk factor for atherosclerotic diseases. Our previous studies have shown that chronic unpredictable mild stress (CUMS) accelerates atherosclerosis and up-regulates TLR4/NF-kappa B expression in apoE(-/-) mice. However, TLR4/NF-kappa B signaling whether directly contributes to the development of atherosclerosis in CUMS mice is unclear. We hypothesized that the interference of TLR4/NF-kappa B can ameliorate CUMS-induced inflammation and atherosclerosis in apoE(-/-) mice. Methods ApoE(-/-) mice were exposed to 12 weeks CUMS. Ad-siRNA TLR4 was given by tail vein injection (10 mu l/mouse, every 5 days), and PDTC (an inhibitor of NF-kappa B) was given by intraperitoneal injection (60 mg/kg, once a day). Plasma corticosterone concentrations were determined by solid-phase I-125 radioimmunoassay. Atherosclerosis lesions in aortic sinuses were evaluated and quantified by IMAGEPRO PLUS. Western blotting was used to detect the expression of TLR4, NF-kappa B, and IL-1 beta in aortas of the mice. Plasma lipid profiles, IL-1 beta, TNF-alpha, and MCP-1 were measured by ELISA. Results Our results indicated that CUMS apoE(-/-) mice treatment with siRNA TLR4 significantly decreased atherosclerosis and down-regulated TLR4, NF-kappa B, and inflammatory cytokines. PDTC also remarkably reduced atherosclerosis and the levels of IL-1 beta, TNF-alpha and MCP-1 in plasma. However, Treatment with siRNA TLR4 or PDTC had no effect on plasma corticosterone levels, and lipid profiles. Conclusions TLR4/NF-kappa B pathway may participate in CUMS-induced atherosclerosis through activation of proinflammatory cytokines in apoE(-/-) mice. Our data may provide a new potential therapeutic target for prevention of CUMS-induced atherosclerosis.
摘要：
P63 null mice have no or truncated limbs and mutations in human p63 cause several skeletal syndromes that also show limb and digit abnormalities, suggesting its essential role in bone development. In the current study, we investigated the effect of ATRA on chondrogenesis using mesenchymal cells from rat hind limb bud and further examined the mRNA and protein expression of Sox9 and Col2a1 and p63 in rat hind limb bud cells. Limb buds were isolated from embryos from euthanized female rats. Growth of hind limb bud mesenchymal cells was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assays. Formation of cartilage nodules was examined by Alcian blue-nuclear fast red staining. The expression of Sox9, Col2al and p63 was determined by Real-time RT-PCR and immunoblotting assays, respectively. Our MTT assays revealed that ATRA at 1 and 10 mu M significantly suppressed the growth of mesenchymal cells from rat hind limb bud at 24 and 48 h (P <0.01 vs. controls). Alcian blue staining further showed that ATRA caused a significant dose-dependent reduction in the area of cartilage nodules (P <0.05 in all vs. controls). At 1 mu M ATRA, the area of cartilage nodules from hind limb bud cells was reduced to 0.05 +/- 0.03 mm from 0.15 +/- 0.01 mm in controls. Real-time RT-PCR assays further indicated that 1 and 10 mu M ATRA markedly reduced the mRNA expression of Sox9, Col2al and p63 in hind limb bud cells (P < 0.05 in all vs. controls). In addition, ATRA time-dependently inhibits the mRNA expression of p63, Sox9 and Col2al. Western blotting assays additionally showed that ATRA dose-dependently reduced the expression of Sox9, Col2al and p63 (P < 0.05 in all vs. controls). Together, our results suggest that ATRA suppresses chondrogenesis by modulating the expression of Sox9, Col2al and p63 in primary hind limb bud mesenchymal cells. (C) 2014 Elsevier B.V. All rights reserved.
摘要：
Attachments of Acidithiobacillus ferrooxidans ATCC 23270 onto elemental sulfur, quartz and complex chalcopyrite were investigated by analysis of its extracellular polymeric substances as well as applying Langmuir and Freundlich equations. The two equations fitted the adsorption equilibrium data with significant correlation coefficient over 0.9. This indicated that bacterial attachment is complicated and involves Langmuir and Freundlich characterizations. Sulfur-grown cells showed the highest affinity for the three solid substrates. The investigated complex chalcopyrite possessed a higher maximum adsorption capacity for A. ferrooxidans than elemental sulfur or quartz. The Freundlich fitting parameters suggested that quartz had a weaker adsorption capacity and smaller adsorption areas than elemental sulfur or the complex chalcopyrite. It is not the content of total carbohydrates or proteins in EPS but their ratios that determine the affinity differences between cells and substrates.
