期刊：
Molecular and cellular biochemistry,2016年414(1-2):57-66 ISSN：0300-8177
通讯作者：
Tang, Zhi-Han;Jiang, Zhi-Sheng
作者机构：
[Liu, Lu-Shan; Li, Guo-Hua; Qu, Shun-Lin; Ren, Zhong; Tang, Hui; Luo, Xin-Yuan; Peng, Juan; Peng, Li-Jun; Liu, Mi-Hua] Institute of Cardiovascular Disease and Key Lab for Arteriosclerology of Hunan Province, University of South China, 28 W Changsheng Road, Hengyang, 421001, Hunan, People's Republic of China;[Zhang, Yuan] Department of Pathology, Affiliated Mawangdui Hospital, University of South China, Changsha, Hunan, 410016, People's Republic of China;[Jiang, Zhi-Sheng] Institute of Cardiovascular Disease and Key Lab for Arteriosclerology of Hunan Province, University of South China, 28 W Changsheng Road, Hengyang, 421001, Hunan, People's Republic of China. zsjianglab@aliyun.com;[Liu, Yao] Chuanshan College, University of South China, Hengyang, 421001, Hunan, People's Republic of China;[Peng, Li-Jun] Department of Science and Education, The Pediatric Academy of University of South China, Changsha, Hunan, 410007, People's Republic of China
通讯机构：
[Jiang, ZS] Univ South China, Key Lab Arteriosclerol Hunan Prov, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.;Institute of Cardiovascular Disease and Key Lab for Arteriosclerology of Hunan Province, University of South China, 28 W Changsheng Road, Hengyang, 421001, Hunan, People's Republic of China.
摘要：
Cardiovascular disease is a growing major global public health problem. Oxidative stress is regarded as one of the key regulators of pathological physiology, which eventually leads to cardiovascular disease. However, mechanisms by which FGF-2 rescues cells from oxidative stress damage in cardiovascular disease is not fully elucidated. Herein this study was designed to investigate the protective effects of FGF-2 in H2O2-induced apoptosis of H9c2 cardiomyocytes, as well as the possible signaling pathway involved. Apoptosis of H9c2 cardiomyocytes was induced by H2O2 and assessed using methyl thiazolyl tetrazolium assay, Hoechst, and TUNEL staining. Cells were pretreated with PI3K/Akt inhibitor LY294002 to investigate the possible PI3K/Akt pathways involved in the protection of FGF-2. The levels of p-Akt, p-FoxO3a, and Bim were detected by immunoblotting. Stimulation with H2O2 decreased the phosphorylation of Akt and FoxO3a, and induced nuclear localization of FoxO3a and apoptosis of H9c2 cells. These effects of H2O2 were abrogated by pretreatment with FGF-2. Furthermore, the protective effects of FGF-2 were abolished by PI3K/Akt inhibitor LY294002. In conclusion, our data suggest that FGF-2 protects against H2O2-induced apoptosis of H9c2 cardiomyocytes via activation of the PI3K/Akt/FoxO3a pathway.
作者机构：
[Peng, Juan; Tang, Ya Ling; Wang, Shuang; Gu, Hong-Feng; Yang, Yong-Zong] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Peoples R China.;[Jiang, Jian Hong; Liu, Zhu] Univ South China, Chuanshan Coll, Hengyang 421001, Peoples R China.;[Tang, Xiao Qing; Gu, Hong-Feng] Univ South China, Dept Physiol, Hengyang 421001, Peoples R China.;[Tang, Xiao Qing; Gu, Hong-Feng] Univ South China, Inst Neurosci, Hengyang 421001, Peoples R China.
通讯机构：
[Gu, HF] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Peoples R China.
摘要：
Objective Chronic stress is an important risk factor for atherosclerotic diseases. Our previous studies have shown that chronic unpredictable mild stress (CUMS) accelerates atherosclerosis and up-regulates TLR4/NF-kappa B expression in apoE(-/-) mice. However, TLR4/NF-kappa B signaling whether directly contributes to the development of atherosclerosis in CUMS mice is unclear. We hypothesized that the interference of TLR4/NF-kappa B can ameliorate CUMS-induced inflammation and atherosclerosis in apoE(-/-) mice. Methods ApoE(-/-) mice were exposed to 12 weeks CUMS. Ad-siRNA TLR4 was given by tail vein injection (10 mu l/mouse, every 5 days), and PDTC (an inhibitor of NF-kappa B) was given by intraperitoneal injection (60 mg/kg, once a day). Plasma corticosterone concentrations were determined by solid-phase I-125 radioimmunoassay. Atherosclerosis lesions in aortic sinuses were evaluated and quantified by IMAGEPRO PLUS. Western blotting was used to detect the expression of TLR4, NF-kappa B, and IL-1 beta in aortas of the mice. Plasma lipid profiles, IL-1 beta, TNF-alpha, and MCP-1 were measured by ELISA. Results Our results indicated that CUMS apoE(-/-) mice treatment with siRNA TLR4 significantly decreased atherosclerosis and down-regulated TLR4, NF-kappa B, and inflammatory cytokines. PDTC also remarkably reduced atherosclerosis and the levels of IL-1 beta, TNF-alpha and MCP-1 in plasma. However, Treatment with siRNA TLR4 or PDTC had no effect on plasma corticosterone levels, and lipid profiles. Conclusions TLR4/NF-kappa B pathway may participate in CUMS-induced atherosclerosis through activation of proinflammatory cytokines in apoE(-/-) mice. Our data may provide a new potential therapeutic target for prevention of CUMS-induced atherosclerosis.
作者机构：
[Le-Xian Xia; Guan-Zhou Qiu; Wen-Juan Sun; Zhen Shen] Key Laboratory of Biohydrometallurgy of Ministry of Education, School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083 Hunan, China;[Le-Xian Xia; Tomas Vargas] Centro de Estudios en Hidro/Electrometalurgia, Departamento de Ingenierfa de Minas, Universidad de Chile, Beaucheff 861, Santiago, Chile;[Ren-Man Ruan] Institute of Process Engineering, Chinese Academy of Science, Beijing, 100190, China;[Zhen-Da Xie] Chuanshan College, University of South China, Hengyang, 421001 Hunan, China
通讯机构：
[Xia, Le-Xian] Key Laboratory of Biohydrometallurgy of Ministry of Education, School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, Hunan, China,
摘要：
Attachments of Acidithiobacillus ferrooxidans ATCC 23270 onto elemental sulfur, quartz and complex chalcopyrite were investigated by analysis of its extracellular polymeric substances as well as applying Langmuir and Freundlich equations. The two equations fitted the adsorption equilibrium data with significant correlation coefficient over 0.9. This indicated that bacterial attachment is complicated and involves Langmuir and Freundlich characterizations. Sulfur-grown cells showed the highest affinity for the three solid substrates. The investigated complex chalcopyrite possessed a higher maximum adsorption capacity for A. ferrooxidans than elemental sulfur or quartz. The Freundlich fitting parameters suggested that quartz had a weaker adsorption capacity and smaller adsorption areas than elemental sulfur or the complex chalcopyrite. It is not the content of total carbohydrates or proteins in EPS but their ratios that determine the affinity differences between cells and substrates.
期刊：
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2014年38(2):460-468 ISSN：1382-6689
通讯作者：
Du, Shi-Xin
作者机构：
[Wang, Yun-Gong] Wang Chuanshan College, University of South China, Hengyang, Hunan, 421001, China;[Du, Shi-Xin] Department of Orthopedics, the Second Hospital of Tianjin Medical University, Tianjin, 300211, China. Electronic address: 8760715@163.com;[Wang, Yun-Guo; Xie, Peng] Department of Orthopedics, the Second Hospital of Tianjin Medical University, Tianjin, 300211, China;[Li, Xue-Dong; Zhang, Tao-Gen; Liu, Zhao-Yong; Hong, Quan] Department of Orthopedics, the First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, 515041, China
通讯机构：
[Du, Shi-Xin] Department of Orthopedics, the Second Hospital of Tianjin Medical University, Tianjin, 300211, China. Electronic address:
摘要：
P63 null mice have no or truncated limbs and mutations in human p63 cause several skeletal syndromes that also show limb and digit abnormalities, suggesting its essential role in bone development. In the current study, we investigated the effect of ATRA on chondrogenesis using mesenchymal cells from rat hind limb bud and further examined the mRNA and protein expression of Sox9 and Col2a1 and p63 in rat hind limb bud cells. Limb buds were isolated from embryos from euthanized female rats. Growth of hind limb bud mesenchymal cells was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assays. Formation of cartilage nodules was examined by Alcian blue-nuclear fast red staining. The expression of Sox9, Col2al and p63 was determined by Real-time RT-PCR and immunoblotting assays, respectively. Our MTT assays revealed that ATRA at 1 and 10 mu M significantly suppressed the growth of mesenchymal cells from rat hind limb bud at 24 and 48 h (P <0.01 vs. controls). Alcian blue staining further showed that ATRA caused a significant dose-dependent reduction in the area of cartilage nodules (P <0.05 in all vs. controls). At 1 mu M ATRA, the area of cartilage nodules from hind limb bud cells was reduced to 0.05 +/- 0.03 mm from 0.15 +/- 0.01 mm in controls. Real-time RT-PCR assays further indicated that 1 and 10 mu M ATRA markedly reduced the mRNA expression of Sox9, Col2al and p63 in hind limb bud cells (P < 0.05 in all vs. controls). In addition, ATRA time-dependently inhibits the mRNA expression of p63, Sox9 and Col2al. Western blotting assays additionally showed that ATRA dose-dependently reduced the expression of Sox9, Col2al and p63 (P < 0.05 in all vs. controls). Together, our results suggest that ATRA suppresses chondrogenesis by modulating the expression of Sox9, Col2al and p63 in primary hind limb bud mesenchymal cells. (C) 2014 Elsevier B.V. All rights reserved.
期刊：
Zhongguo Youse Jinshu Xuebao/Chinese Journal of Nonferrous Metals,2015年25(11):3237-3245 ISSN：1004-0609
通讯作者：
Peng, Guo-Wen(pgwnh@sohu.com)
作者机构：
[ Zhang, Zhi-Jun ; Ding, De-Xin ; Peng, Guo-Wen ; Xiao, Fang-Zhu ; Hu, Nan ] Key Discipline Laboratory for National Defence for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, China;[Peng, Guo-Wen ; Wang, Xiao-Liang ] School of Chemistry and Chemical Engineering, University of South China, Hengyang, China;[ Zhou, Gui ; Huang, Hong ; Yang, Jin-Ran ] Wang Chuanshan College, University of South China, Hengyang, China;[Peng, Guo-Wen ] School of Resources and Safety Engineering, Central South University, Changsha, China
关键词：
Adsorption isotherm model - Adsorption kinetics - Adsorption temperature - Correlation coefficient - Functional modification - Initial concentration - Mesoporous Silica - Pseudo-second order model
期刊：
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2013年40(09):834-844 ISSN：1000-3282
通讯作者：
Gu, HF
作者机构：
[Liao Duanfang; Tong Qiaozhen] Hunan Univ Chinese Med, Div Stem Cell Regulat & Applicat, State Key Lab Chinese Med Powder & Med Innovat Hu, Changsha 410208, Hunan, Peoples R China.;[Jiang Jianhong] Nanhua Univ, Chuanshan Coll, Hengyang 421001, Peoples R China.;[Yang Yongzong; Gu Hongfeng] Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Peoples R China.;[Gu Hongfeng] Univ South China, Dept Physiol, Hengyang 421001, Peoples R China.
通讯机构：
[Gu, HF] Univ South China, Dept Physiol, Hengyang 421001, Peoples R China.
关键词：
GP Ⅱb/Ⅲa;atherosclerosis;HMGB-1;TLR4;NF-κB
摘要：
The effects of glycoprotein (GP) Ⅱb/Ⅲa inhibitors on the development of the atherosclerotic process has received scant attention. To investigate whether GP Ⅱb/Ⅲa blockade influences atherosclerosis lesion and HMGB-1/TLR4 signaling, we compared plaque formation in ApoE~(-/-) mice: control group (n=10); IgG group (n=10, 50 μg) and GP Ⅱb/Ⅲa mAb group (n=10, 50 μg). All mice were fed on a Western diet (10% fat and 1.25% cholesterol) for 10 weeks. GP Ⅱb/Ⅲa blockade significantly decreased the atherosclerotic lesion and platelet adhesion to the vessel wall. Immunohistochemistry analysis showed that blocking GP Ⅱb/Ⅲa diminished MOMA-2 and VCAM-1 expression in aortic plaque in ApoE~(-/-) mice. Western blot results indicated that HMGB-1, TLR4, and NF-κB levels were markedly reduced in arteries of ApoE-/- mice treated with GP Ⅱb/Ⅲa mAb (P < 0.05). Moreover, GP Ⅱb/Ⅲa mAb decreased plasma HMGB-1, IL-1β, TNF-α and MCP-1 concentrations. Our findings demonstrated that GP Ⅱb/Ⅲa mAb significantly decreased atherosclerotic lesions and HMGB-1, TLR4 and NF-κB expression in ApoE~(-/-) mice (P < 0.05). The present study has suggested a possibility that GP Ⅱb/Ⅲa blockade attenuates atherosclerosis by inhibiting the HMGB-1/TLR4 pathway..
作者机构：
[ Xie, Xionggang ] Mining College, Guizhou University, Guiyang 550004, China;[Wang, Shuyun ] School of Environment Protection and Safety Engineering, University of South China, Hengyang 421001, China;[ Chen, Xi ] Chuanshan College, University of South China, Hengyang, 421001, China
关键词：
Computer aided - Computer techniques - Construction procedures - Design for safety - Elasto-plastic - Finite difference - In-field - Laboratory test - Lagrangian - Large deformation analysis - Numerical simulation method - Practical problems - Rapid development - Rock-mass - Simple geometries - Stratified media - Stratified rock mass - Stratified rocks - Tunnel engineering - Tunnel excavation - Underground excavation
作者机构：
[Jiang, Guan Min] 1.Chuanshan College, University of South China, Hengyang 421001, China 2.School of Civil Engineering, Central South University, Changsha 410004, China 3.Department of Clinical Laboratory, The First Affiliated Hospital of University of South China, Hengyang 421001, China
摘要：
Spectacles are vital for correcting vision. Individuals who need vision correction can choose to wear spectacles or contact lenses. Alternatively, they may choose to have a vision-correcting operation. Therefore, researchers focus on vision science and spectacle design. Although some studies have compared spectacles and contact lenses for better visual quality, there is no consensus regarding which are better for the correction of vision. With the development of technology and the improvement of living standards, the design and production of spectacles will focus on personalized style, lenses, and frames. In this paper, we introduced trends in spectacles and studies of the relationship between frame sizes and face sizes, attempting to provide a feasible program for the personalization of spectacles-the Personalized Spectacles Program. Furthermore, certain limitations, such as non-personalized and misused spectacles, which have elevated the importance of the design and production of personalized spectacles were discussed.
