期刊论文

中文

中国细胞生物学学报

1674-7666

2024

46

02

246-255

10.11844/cjcb.2024.02.0006

本校为第一机构

蛋氨酸亚砜还原酶B（methionine sulfoxide reductase, MsrB）是一种重要的氧化还原蛋白。该文探究了肺炎支原体（Mycoplasma pneumoniae, Mp） MsrB对Mp脂质相关膜蛋白（lipidassociated membrane proteins, LAMPs）刺激的人髓系白血病单核细胞（THP-1细胞）分泌促炎细胞因子肿瘤坏死因子α（TNF-α）和白细胞介素1β（IL-1β）的调节及相关信号通路,以进一步了解Mp的免疫逃避机制。论文构建了pET28a（+）-msr B重组质粒,诱导表达、鉴定、纯化重组蛋白（rMsrB）并制备了多克隆抗体; Western blot检测MsrB、TLR1、TLR2、TLR6和MyD88蛋白表达水平及NF-κB p65、P38、ERK和JNK的总蛋白和磷酸化蛋白...

MsrB(methionine sulfoxide reductase)is an essential redox protein in prokaryotic and eukary-otic cells.This study aimed to investigate the regulation and signaling pathway of MsrB on the secretion of the pro-inflammatory cytokines TNF-α(tumor necrosis factor-alpha)and IL-1β(interleukin 1β)in THP-1 cells(human my-eloid leukemia monocytes)which were stimulated with LAMPs(lipid-associated membrane proteins)derived from Mp(Mycoplasma pneumoniae),so as to further understand the immune evade of Mp.In this study,pET28a(+)-msrB recombinant plasmid w...