期刊论文

Gu Hong-Feng

英文

生物化学与生物物理进展

1000-3282

2013

40

9

834-844

10.3724/SP.J.1206.2012.00602

国家自然科学基金(81173047);湖南省教育厅基金(09C835)资助项目.This work was supported by grants from The National Natural Science Foundation of China(81173047);Department of Education Fund of Hunan Province(09C835)

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The effects of glycoprotein (GP) Ⅱb/Ⅲa inhibitors on the development of the atherosclerotic process has received scant attention. To investigate whether GP Ⅱb/Ⅲa blockade influences atherosclerosis lesion and HMGB-1/TLR4 signaling, we compared plaque formation in ApoE~(-/-) mice: control group (n=10); IgG group (n=10, 50 μg) and GP Ⅱb/Ⅲa mAb group (n=10, 50 μg). All mice were fed on a Western diet (10% fat and 1.25% cholesterol) for 10 weeks. GP Ⅱb/Ⅲa blockade significantly decreased the atherosclerotic lesion and platelet adhesion to...

观察膜糖蛋白（GP） Ⅱb/Ⅲa 单抗对小鼠动脉粥样硬化（atherosclerosis，As）病变和HMGB1/TLR4途径基因表达变化的影响，以探讨膜糖蛋白Ⅱb/Ⅲa 受体拮抗剂对As进程的影响及其机制．30只5周龄雄性ApoE-/-小鼠随机均分为3组：溶剂对照组（生理盐水50 μl，腹腔注射），IgG 对照组（50 μg，腹腔注射），GP Ⅱb/Ⅲa 单抗组（50 μg，腹腔注射）．实验ApoE-/-小鼠均已高脂、高胆固醇饲料喂养，10周后处死动物．油红O染色观察主动脉窦As病变；活体荧光显微镜观察颈总动脉As病变处血小板黏附；Western blot 检测HMGB-1、TLR4与NF-κB蛋白的表达；免疫组化观察主动脉窦As病变部位MOMA-2 和VCAM-1的表...