作者机构：
[刘丽苹; 刘健; 全淑芬; 谢德春; 郝玉娥] Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hunan, Hengyang, 421001, China;[王秋平] The First Affiliated Hospital of South China University, Hunan, Hengyang, 421001, China
作者机构：
[李琪; 罗金定; 王紫涵; 李梦伟; 凌宏艳] Department of Physiology, Hengyang Medical College, China;[陈碾] Department of Medical Cosmetology, The First Affiliated Hospital, China;[伍惠霖] Clinical Medicine Class 10, Grade 2018, Hengyang Medical College, China;[奉水东] Department of Social Medicine and Health Management, School of Public Health, University of South China, Hengyang, 421001, China
摘要：
[目的]分析1990—2019年中国卵巢癌发病与死亡现状及其流行趋势。[方法]收集并整理全球健康数据交换数据库(the Global Health Data Exchange,GHDx)中1990—2019年20~<85岁中国卵巢癌的发病与死亡数据,利用Joinpoint线性回归模型分析卵巢癌发病与死亡的变化趋势,并计算年度变化百分比(APC)与平均年度变化百分比(AAPC)值。运用年龄-时期-队列模型分析年龄、时期、队列对发病率与死亡率变化的影响。[结果] 1990—2019年中国卵巢癌发病率与死亡率均呈上升趋势,分别平均每年上升3.85%、3.86%。模型结果显示,发病与死亡的净漂移值均>0,分别为1.82%、0.89%。发病与死亡风险随年龄和时期的增加而增高,其队列效应表现不同,出生越晚的人群发病风险高,死亡风险低。[结论] 1990—2019年中国卵巢癌总体发病与死亡率均呈上升趋势,生活时代越近的老年人群发病与死亡的风险越高,对卵巢癌的防治工作应加强关注。
作者机构：
[龙鼎新] School of Public Health, Hengyang Medical School, University of South China, Hunan, Hengyang, 421001, China;[李静芸; 陈娜; 贾乐乐; 张忠彬] National Center for Occupational Safety and Health, NHC/NHC Key Laboratory for Engineering Control of Dust Hazard, Beijing, 102308, China;[卜千] School of Public Health, Hengyang Medical School, University of South China, Hunan, Hengyang, 421001, China<&wdkj&>National Center for Occupational Safety and Health, NHC/NHC Key Laboratory for Engineering Control of Dust Hazard, Beijing, 102308, China
作者机构：
[Xie, Min; Sheng, Liping] Hunan Normal Univ, Coll Chem & Chem Engn, Natl Local Joint Engn Lab New Petro Chem Mat & Fi, Changsha 410081, Peoples R China.;[Xie, Min; Huang, Chaobo] Nanjing Forestry Univ NFU, Coll Chem Engn, Nanjing 210037, Peoples R China.;[Xie, Min; Liang, Yongqi; Li, Shuang] Xiangtan Univ, Coll Chem, Key Lab Environm Friendly Chem & Applicat, Lab Biochem,Minist Educ, Xiangtan 411105, Peoples R China.;[Cao, Yi] Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang 421001, Peoples R China.
通讯机构：
[Sheng, Liping] H;[Cao, Yi] U;Hunan Normal Univ, Coll Chem & Chem Engn, Natl Local Joint Engn Lab New Petro Chem Mat & Fi, Changsha 410081, Peoples R China.;Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang 421001, Peoples R China.
关键词：
3D Caco-2 spheroids;Fat digestion and absorption (map04975);Lipidomics;MoS2 nanosheets (NSs);Transcriptomics
摘要：
MoS(2 )nanosheets (NSs) are novel 2D nanomaterials (NMs) with potential uses in many areas, and therefore oral exposure route to MoS2 NSs is plausible. Currently, MoS2 NSs are considered as biocompatible NMs, but there is lacking of systemic investigations to study the interactions of MoS2 NSs with intestinal cells. In this study, we exposed the 3D Caco-2 spheroids to MoS2 NSs or MoS2 powders (denoted as MoS2-bulk), and investigated the potential adverse effects of MoS2-materials based on transcriptomics and lipidomics analysis. As expected, both MoS(2)NSs and MoS2 -bulk were dose-dependently internalized into 3D Caco-2 spheroids but did not induce cytotoxicity, membrane disruption or decrease of thiols. However, the Gene Ontology (GO) and Kyoto Encyclopedia of Gene and Genomes (KEGG) analysis indicated that nutrient absorption and metabolism was decreased. One of the most significantly decreased KEGG pathways is fat digestion and absorption (map04975), and Western blotting analysis further showed that fatty acid binding protein 1 and apolipoprotein Al, key proteins involved in fat digestion and absorption, were down-regulated by MoS2 NSs or MoS2-bulk. In addition, BODIPY 493/503 staining suggested that exposure to MoS2 NSs and MoS2-bulk decreased lipid levels in the spheroids. However, lipidomics data indicated that MoS2 materials only decreased 8 lipid classes, including Iysophosphatidylcholine, Iysodimethylphosphatidylethanolamine, N-acylethanolamine, ceramide phosphoethanolamines, gangliosides, lysosphingomyelin and sulfatide, whereas most of the lipid classes were indeed increased. In addition, MoS2 NSs was more potent to decrease the lipid classes compared with MoS2 -bulk. Combined, the results from this study showed that MoS2 NSs and bulk materials were non-cytotoxic but altered lipid profiles in 3D Caco-2 spheroids. (C) 2021 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
作者机构：
[Dingxin Long] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China;[Qiang Xiong; Fei Chen; Jing Liang; Zhen Zhang] National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China;[Hailan Yang] China Institute of Radiation Protection, Taiyuan, 030006, China;[Xiao Ge; Linlin Yan] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China<&wdkj&>National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China
通讯机构：
[Fei Chen] N;[Dingxin Long] H;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China<&wdkj&>National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China
摘要：
Objective: To establish a rapid analysis method for the activity concentration of carbon-14 (~(14)C) in urine, in order to estimate the internal dose of ~(14)C exposure, and to protect the health of occupational population. Methods: Liquid scintillation counting(LSC) combined with the function of transformed spectral index of the external standard spectrum (tSIE) was used to measure the quenching level and counting rate, and the spiked urine samples with different shades of color were measured by LSC. After establishing the efficiency-quenching curve, the quenching correction and activity concentration analysis of the actual samples were carried out. Results: By LSC and the data fitting, the relationship between efficiency and quenching index could be represented using the equation y = 0.0013x-0.0177 (R~2 = 0.978). Three actual spiked samples were tested to verify this method, with recoveries of 97%, 102%, and 89%, respectively. ~(14)C activity concentration of 4 actual urine samples were 0.12, 0.11, 0.10 and 0.08 Bq/mL, respectively, while the corresponding extended relative uncertainties were 0.0652, 0.0929, 0.0893 and 0.1043, respectively. Conclusion: The direct analysis method of ~(14)C activity concentration in urine samples was established using LSC. The recovery of ~(14)C activity concentration in urine samples showed that the proposed method had relatively high accuracy. By studying on the source of uncertainty, the uncertainty of the analysis results mainly came from the statistical error of LSC, and the uncertainty component of counting efficiency.
作者机构：
[李刚; 赖淑婷] School of Public Health, University of South China，Hengyang421001，, China;[高山山; 周平坤; 韩阳; 关华; 白琛俊] Institute of Radiation Medicine，Academy of Military Medical Sciences，Academy of Military Sciences, Beijing Key iMboratory for Radiobiology, Beijing, 100850, China
通讯机构：
[Gao, S.] I;Institute of Radiation Medicine，Academy of Military Medical Sciences，Academy of Military Sciences, China
