期刊论文

中文

南方医科大学学报

1673-4254

2023

43

2

294-299

10.12122/j.issn.1673-4254.2023.02.19

国家自然科学基金(81572011) 湖南省自然科学基金(2020JJ4527) 湖南省教育厅重点项目(20A438)。

本校为第一机构

目的 探讨鹦鹉热衣原体（Cps）SINC蛋白对宿主细胞自噬的影响，及MAPK/ERK信号通路在其中的调控作用。方法 用重组的SINC蛋白刺激小鼠单核巨噬细胞（RAW 264.7）,Western blot检测LC3-Ⅱ和Beclin-1的水平及ERK1/2的磷酸化程度，并用间接免疫荧光法检测SINC蛋白刺激后细胞LC3的水平，透射电镜检测自噬特殊结构。用50μmol MEK1/2抑制剂U0126预处理RAW 264.7细胞后1 h，再用不同浓度SINC蛋白刺激不同时间，用间接免疫荧光检测LC3的水平，并用Western blot检测LC3-Ⅱ和Beclin-1的表达水平。结果 Western blot检测结果显示，以2μg/mL SINC蛋白刺激RAW 264.7细胞12 h时，LC3-Ⅱ和Beclin-1的表达水平...

Objective To investigate the effects of SINC, a secreted protein of Chlamydia psittaci, on autophagy of host cells and the role of MAPK/ERK signaling pathway in mediating SINC-induced autophagy. Methods RAW 264.7 cells treated with recombinant SINC were examined for cha...MORE Objective To investigate the effects of SINC, a secreted protein of Chlamydia psittaci, on autophagy of host cells and the role of MAPK/ERK signaling pathway in mediating SINC-induced autophagy. Methods RAW 264.7 cells treated with recombinant SINC were examined for chang...