期刊论文

Gao, S.

中文

中华放射医学与防护杂志

0254-5098

2022

42

4

241-247

10.3760/cma.j.cn112271-20210930-00400

国家自然科学基金（31870847, 32101165）；

本校为第一机构

目的:探讨TAB182促进同源重组(HR)修复的相关调控分子及机制。方法:利用shRNA敲低人乳腺癌MCF-7细胞中的TAB182基因，TAB182敲低的MCF-7细胞为沉默组，使用shRNA阴性对照物的MCF-7细胞为TAB182阴性对照组。通过RNA测序分析，筛选与TAB182相关差异表达的HR修复基因。qRT-PCR验证相关基因的mRNA表达，Western blot验证相关蛋白的表达。RAD51和BrdU免疫荧光检测DNA断裂末端形成的3′单链DNA(ssDNA)；流式细胞术检测细胞周期阻滞和细胞凋亡；集落形成实验检测细胞的放射敏感性。结果:RNA测序分析和qRT-PCR验证结果一致，TAB182沉默组细胞中RPA2的mRNA表达降低( t＝17.97， P

Objective:To investigate the regulating molecules and acting mechanism of TAB182 in HR pathway.Methods:TAB182 in human breast cancer MCF-7 cells was knocked down by shRNA strategy, the TAB182 knockdown MCF-7 as the TAB182 knockdown group, and the MCF-7 cell using the shRNA negative control as the TAB182 negative control group. RNA sequencing and qRT-PCR were performed to screen and verify the differentially expressed genes of HR pathway related to TAB182 depression. Western blot was used to detect protein expression. Immunofluorescence staining...