作者机构:
[乃爱桃; 艾小红] Department of Radiation Oncology, The First Affiliated Hospital of University of South China, Hengyang, 421001, China;[王五洲; 陈熙; Wan W.; 曹文宇; 王逸轩] Clinical Anatomy & Reproductive Medicine Application Institute, Medical College, University of South China, Hengyang, 421001, China;[罗丹; 何洁] Department of Pathology, Medical College, University of South China, Hengyang, 421001, China;[万炜; 何淑雅] Department of Radiation Medicine, School of Public Health, University of South China, Hengyang, 421001, China;[徐杨] Department of Physiology, Medical College, University of South China, Hengyang, 421001, China
通讯机构:
[Xu, Y.; Ai, X.] D;Department of Physiology, China;Department of Radiation Oncology, China
关键词:
电离辐射;前额叶皮质区;NLRP3炎性小体;认知功能障碍
摘要:
探讨电离辐射诱导小鼠认知功能障碍的可能机制.20只雌性昆明小鼠随机分为对照组和辐照组,辐照组小鼠接受137Cs γ射线单次全身辐照至吸收剂量4 Gy.35 d后采用新旧事物识别实验检测小鼠认知功能;免疫组织化学法检测小鼠前额叶皮质区小胶质细胞标记物离子钙接头蛋白-1(IBA-1)的表达;蛋白质印迹法检测前额叶皮质区核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、凋亡相关斑点样蛋白(ASC)、半胱氨酸天门冬氨酸蛋白酶-1(Caspase-1)、白细胞介素-1β(IL-1 β)和白细胞介素-18(IL-18)蛋白的表达变化;荧光定量PCR(RT-PCR)检测前额叶皮质IBA-1、NLRP3、ASC和Caspase-1 mRNA的表达情况.结果显示:与对照组相比,辐照组小鼠在新旧事物识别实验中对新事物的分辨率明显降低(38.39 ± 3.69 vs. 28.82 ± 2.08, p<0.05);前额叶皮质区IBA-1阳性细胞数(138.2 ± 3.7 vs. 159.6 ± 6.9, p<0.05)和mRNA表达(1.000 ± 0.031 vs. 1.173 ± 0.055, p<0.05)均显著上调;前额叶皮质区NLRP3、ASC、Caspase-1蛋白(1.000 ± 0.066 vs. 1.341 ± 0.119, p<0.05;1.000 ± 0.073 vs. 1.298 ± 0.083, p<0.05;1.000 ± 0.039 vs. 1.603 ± 0.159, p<0.01)及NLRP3、 ASC mRNA表达(1.000 ± 0.046 vs. 1.372 ± 0.071, p<0.01; 1.000 ± 0.068 vs. 1.225 ± 0.069, p<0.05)均明显上调;前额叶皮质区炎性细胞因子IL-1β和IL-18的表达(1.000 ± 0.033 vs. 1.167 ± 0.059, p<0.05;1.000 ± 0.196 vs. 1.614 ± 0.163, p<0.05)均明显上调.结果提示,电离辐射可能是通过激活前额叶皮质区小胶质细胞,活化NLRP3炎性小体,诱导炎症因子释放引起认知功能障碍的.
摘要:
Dear Editor, The DNA-dependent protein kinase catalytic subunit(DNA-PKcs)forms a serine/threonine protein kinase complex with the Ku heterodimer(Ku70/Ku80)and plays an important role in the DNA damage response(DDR)and maintenance of genomic stability through nonhomologous end joining(NHEJ), wherein the Ku heterodimer recognizes and binds broken DNA ends, facilitating the recruitment and activation of DNA-PKcs. Activated DNA-PKcs phosphorylates and alters the function of factors that mediate NHEJ, including DNA-PKcs itself. In addition, DDR-independent roles of DNA-PKcs have been demonstrated. Studies further identified DNA-PKcs as a modulator of cancer-associated pathways distinct from DNA repair, including hypoxia, metabolism, the inflammatory response, and transcriptional regulation.
作者机构:
[罗金定; 伍迪; Lyu H.-J.; 奉水东; 杨丝丝; 许拓; 何剑琴] Dept of Physiology, Hengyang Medical College, University of South China, Hengyang Hunan, 421000, China;Dept of Physiology, Xiangya Medical College, Central South University, Changsha, 410000, China;Dept of Social Medicine and Health Management, School of Public Health, University of South China, Hengyang Hunan, 421000, China;[凌宏艳] Dept of Physiology, Xiangya Medical College, Central South University, Changsha, 410000, China, Dept of Social Medicine and Health Management, School of Public Health, University of South China, Hengyang Hunan, 421000, China
作者机构:
[Ma Quan; Xu Xin Yun; Wang Bing Yu; Tan Qin; Qin Xiao Yun; Zheng Kai] Shenzhen Ctr Dis Control & Prevent, Inst Toxicol, Shenzhen 518055, Guangdong, Peoples R China.;[Wang Bing Yu; Zheng Kai] Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Xu Xin Yun] S;Shenzhen Ctr Dis Control & Prevent, Inst Toxicol, Shenzhen 518055, Guangdong, Peoples R China.
关键词:
PROTEIN;EXPRESSION;Hormone-regulated
摘要:
Phthalates are multifunctional compounds that are used in various commercial and industrial products,such as food packagings,toys,cosmetics,building materials,and personal care products[1-3],Di(2-ethylhexyl) phthalate (DEHP),one of the most commonly used phthalates,is ubiquitously present in humans[4].Studies have shown that DEHP has some potentially adverse effects on health,including abnormal timing of parturition,reproductive dysfunction,and low birth weight[5-7].Some authors reported that DEHP affects the function of the hypothalamus-pituitary-ovarian axis and influences the mRNA and protein levels of GnRHR in the pituitary[8].Furthermore,limited studies have shown that DEHP affects oocyte maturation by modulating the expression of luteinizing hormone receptor (LHR)[9-10].In this study,we explored the effects of DEHP exposure on ovarian histology and ultra-structure,and then we studied the possible involvement of oncogenes and apoptosis-related genes mechanisms after DEHP treatment were studied,then we attempted to explore the role of LHR and GnRHR in the ovary.
作者机构:
[Chenqi Y.; Weufing R.] School of Public Health, University of South China, Hengyang, 421001, China;[Haican L.; MacHao L.; Tongyarig X.; Kanglin W.; Ruibai W.; Yi J.; Sfuqteng Y.] State Key Laboratory for Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Beijing, 102206, China
通讯机构:
[Kanglin, W.] S;State Key Laboratory for Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Beijing, China