摘要：
The purpose of this study was to construct and purify a fusion expression and purification of the protein CFP10-ESAT6(CE)of Mycobacterium tuberculosis(MTB)and then preliminarily evaluated its immune effect.The recombinant plasmid pET32a(+)-CE was constructed,with which CE was expressed in E.coli BL21(DE3)and purified by Ni-NTA chromatography.BALB/c mice were immunized with CE adjuvanted with aluminum for three times,the dosage of CE was 50μg per mouse per time.Serum IgG antibodies were determined by ELISA;cytokines were detected by ELISpot and Luminex;In vitro growth inhibition test of MTB was performed.BCG was used as the positive control.CE fusion protein immunized group induced high expression of IgG,IgG1 and IgG2a,and the levels of IgG1(t=19.1,P<0.0001)and IgG2a(t=8.7,P<0.0001)antibodies in group CE were significantly higher than those in group BCG.No statistical difference was found in the number of IFN-γ-secreting spot-forming cells(SFC)induced by the CE and BCG groups(t=0.4,P>0.05),while the number of IL-4-secreting SFCs induced by group CE was significantly higher than that by group BCG(t=8.0,P<0.0001).The levels of GM-CSF(t=8.4,P<0.05),IL-6(t=8.3,P<0.05),IL-10(t=2.5,P<0.05)and IL-4(t=7.0,P<0.05)induced by the CE group were significantly higher than those induced by the BCG group,however,no statistically significant difference was found in the levels of IFN-γ(t=1.4,P>0.05),TNF-α(t=1.8,P>0.05),IL-2(t=2.0,P>0.05)、IL-12(t=0.9,P>0.05)和IL-17(t=1.3,P>0.05)induced by these two groups.In vitro growth inhibition of MTB with spleen cells of the mice in the CE group was similar to that in the BCG group(t=0.8,P>0.05).Mice immunized with CE can induce a strong Th1/Th2-mixed-type immune response,and had a strong ability to inhibit the growth of MTB in vitro,indicating that CE has a good value in the development and application of new tuberculosis vaccine.
作者机构：
[Fei Chen; Qiang Xiong; Zhen Zhang; Jingguang Fan] National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China;[Fei Gao; Feng Zhang] Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100123, China;School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China;[Linlin Yan; Xiao Ge] National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China<&wdkj&>School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China
通讯机构：
[Fei Chen; Jingguang Fan] N;National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China<&wdkj&>National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China
摘要：
Objective: To explore the separation condition for ~(90)Sr using crown ether resin, in order to establish a fast method for analysis of ~(90)Sr in food and environmental samples. Methods: Stable strontium isotope (~(88)Sr) was used to simulate the radioactive ~(90)Sr, and the concentration of ~(88)Sr in elution solution was detected using inductively coupled plasma mass spectrometry (ICP-MS). The separation conditions were investigated through batch experiment. Results: Under the optimized experimental conditions, the maximum adsorption capacity of crown ether resin was 22.28 mg/g. 4.0-8.0 mol/L nitric acid made it easier for the Sr~(2+) to load on the resin, and 20 mL ultrapure water was used to rinse the chromatographic column to achieve a good separation effect, by which the recoveries of Sr~(2+) were nearly close to 100%. The average recovery rates of 4 samples were 60.25%, 69.26%, 75.09% and 66.33%, respectively. Conclusions: Crown ether resin has very good selectivity and adsorption for ~(90)Sr. The optimized conditions were high-efficiency and easy to operate. The separation method has great potential for the fast analysis of ~(90)Sr in food and environmental samples.
作者机构：
[王紫涵; 何剑琴; 凌宏艳; 罗金定; 杨丝丝; 李梦伟] Hengyang Medical College, University of South China, Dept of Physiology,, Hunan, Hengyang, 421001, China;Physical Education Institute, Jishou University, Xiangxi, Hunan416000, China;[丁星星] Chuanshan College University of Sowth China, Hunan, 421001, China;[奉水东] Public Health College, University of South China.Hengyang, Dept of Social Medicine and Health Management,, Hunan, 421001, China;[Lyu H.-J.] Hengyang Medical College, University of South China, Dept of Physiology,, Hunan, Hengyang, 421001, China, Physical Education Institute, Jishou University, Xiangxi, Hunan416000, China
通讯机构：
[Hu, CG ; Naranmandura, H ; Hsu, CH ] Z;Zhejiang Univ, Affiliated Hosp 1, Dept Publ Hlth, Sch Med, Hangzhou 310058, Peoples R China.;Zhejiang Univ, Affiliated Hosp 1, Dept Hematol, Sch Med, Hangzhou 310058, Peoples R China.;Zhejiang Univ, Canc Ctr, Hangzhou 310058, Peoples R China.;Zhejiang Prov Ctr Dis Control & Prevent, Hangzhou 310051, Peoples R China.
摘要：
Despite extensive efforts, COVID-19 pandemic caused by the SARS-CoV-2 virus is still at large. Vaccination is an effective approach to curb virus spread, but several variants (e.g., delta, delta plus, omicron, and IHU) appear to weaken or possibly escape immune protection. Thus, novel and quickly scalable approaches to restrain SARS-CoV-2 are urgently needed. Multiple evidences showed thermal sensitivity of SARS-CoV-2 and negative correlation between environmental temperature and COVID-19 transmission with unknown mechanism. Here, we reveal a potential mechanism by which mild heat treatment destabilizes the wild-type RNA-dependent RNA polymerase (also known as nonstructural protein 12 (NSP12)) of SARS-CoV-2 as well as the P323L mutant commonly found in SARS-CoV-2 variants, including omicron and IHU. Mechanistically, heat treatment promotes E3 ubiquitin ligase ZNF598-dependent NSP12 ubiquitination leading to proteasomal degradation and significantly decreases SARS-CoV-2 RNA copy number and viral titer. A mild daily heat treatment maintains low levels of both wild-type and P323L mutant of NSP12, suggesting clinical potential. Collectively, this novel mechanism, heat-induced NSP12 degradation, suggests a prospective heat-based intervention against SARS-CoV-2.
作者机构：
[Xu, XY; Zhang Zhao Hui; Xu Xin Yun; Liu Yan Ping; Wang Ye; Cai Ying; Lei Yuan Di; Zheng Kai] Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Xu Xin Yun; Cai Ying; Zheng Kai] Shenzhen Ctr Dis Control & Prevent, Shenzhen 518055, Guangdong, Peoples R China.;[Liu Yan Ping; Wang Ye; Zhang Zhao Hui; Cai Ying; Lei Yuan Di] Hunan Key Lab Typical Environm Pollut & Hlth Haza, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Xu, XY; Zhang, ZH] U;[Xu Xin Yun] S;[Zhang Zhao Hui] H;Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;Shenzhen Ctr Dis Control & Prevent, Shenzhen 518055, Guangdong, Peoples R China.
关键词：
ELEMENT;EXPOSED;EXPRESS
摘要：
Beryllium (Be) is a non-radioactive element with carcinogenic properties, and presents serious occupational and environmental hazards from its resulting toxicants, which could significantly impact the health of the occupational population[1-3]. Beryllium and its compounds can cause diseases such as acute chemical pneumonia, lung cancer, and chronic beryllium disease, which predominantly occurs alongside either lung granuloma or pulmonary fibrosis[4,5]. Furthermore, the mechanism by which beryllium is toxic has not been fully elucidated; therefore, studies on its health hazards as well of its compounds are important. An in-depth study of the mechanism of beryllium toxicity is especially essential to prevent and control its associated health hazards. Tandem mass tag (TMT) technology is a reliable technology in quantitative proteomics, which can be implemented to perform relative quantification and identification analysis of proteins, peptides, nucleic acids, and other biological macromolecules.
