摘要：
Objectives The Shh pathway may shed new light on developing new cell death inhibitors for the therapy of ischemic stroke. We aimed to examine whether the Shh co-reporter SMO or its agonist halcinonide can upregulate Bcl-2 to suppress neuronal cell death, ultimately improving behavioral deficits and reducing cerebral infarction in an ischemic stroke model.
The Shh pathway may shed new light on developing new cell death inhibitors for the therapy of ischemic stroke. We aimed to examine whether the Shh co-reporter SMO or its agonist halcinonide can upregulate Bcl-2 to suppress neuronal cell death, ultimately improving behavioral deficits and reducing cerebral infarction in an ischemic stroke model.
Methods Halcinonide or genetic manipulation of SMO was conducted in PC12 cells to examine their impacts on oxidative or OGD/R stress, and the chemical, along with AAV- SMO or AAV- EGFP were tested in MCAO rats to investigate their potential protective effects against neuronal damages due to cerebral I/R injury. The amounts or activities of L-LA, LDH, ROS, MDA, SOD, MPO, GSSG, and GSH were detected using the corresponding biochemical kits. The levels of TNF-α and IL-6 were analyzed by ELISA.
Halcinonide or genetic manipulation of SMO was conducted in PC12 cells to examine their impacts on oxidative or OGD/R stress, and the chemical, along with AAV- SMO or AAV- EGFP were tested in MCAO rats to investigate their potential protective effects against neuronal damages due to cerebral I/R injury. The amounts or activities of L-LA, LDH, ROS, MDA, SOD, MPO, GSSG, and GSH were detected using the corresponding biochemical kits. The levels of TNF-α and IL-6 were analyzed by ELISA.
Results The results show that halcinonide alleviated neurological score and cerebral infarction, and the abnormal changes in L-LA, LDH, MDA, SOD, MPO, GSH, GSSG, TNF-α, and IL-6 were also reversed in MCAO rats. Through expression or knockout of SMO , we discovered that SMO worked similarly to halcinonide, protecting neuronal cells from oxidative or OGD/R stress, and AAV- SMO prevented cerebral damages of MCAO rats caused by ischemia and reperfusion. Halcinonide inhibited Bcl-2/Bax-mediated apoptosis, at least partially by promoting the Shh signaling pathway through enhancing SMO expression in vivo and in vitro.
The results show that halcinonide alleviated neurological score and cerebral infarction, and the abnormal changes in L-LA, LDH, MDA, SOD, MPO, GSH, GSSG, TNF-α, and IL-6 were also reversed in MCAO rats. Through expression or knockout of SMO , we discovered that SMO worked similarly to halcinonide, protecting neuronal cells from oxidative or OGD/R stress, and AAV- SMO prevented cerebral damages of MCAO rats caused by ischemia and reperfusion. Halcinonide inhibited Bcl-2/Bax-mediated apoptosis, at least partially by promoting the Shh signaling pathway through enhancing SMO expression in vivo and in vitro.
Conclusion This study identified a new target and a candidate chemical for therapy of ischemic stroke, hopefully reducing its morbidity and mortality.
This study identified a new target and a candidate chemical for therapy of ischemic stroke, hopefully reducing its morbidity and mortality.
摘要：
BACKGROUND AND AIM: Long term high-dose erythropoietin (EPO) had been reported inducing the formation of abdominal aortic aneurysm (AAA) in mice. When using this model, we found that EPO treated mice showed significant splenomegaly. This is an interesting phenomenon, and its mechanism has not been reported. Therefore, this study aims to explore its mechanism. METHODS: C57BL/6 mice were given intraperitoneal injection of recombinant human EPO at 10000 IU/kg/day, and the control mice were treated with normal saline (vehicle). After 3 weeks, the spleens were harvested. Pathological changes in histology were observed using Hematoxylin and Eosin (H&E) staining. The differential expression genes (DEGs) were identified using RNA sequencing (RNA-Seq), verified with the real-time quantitative polymerase chain reaction (RT-qPCR). The functional-enrichment analysis including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome enrichment analysis were performed to reveal the functional characteristics and related biological pathways of DEGs. Immunohistofluorescence (IHF) and flow cytometry (FCM) were used to detect immune cell subsets and proliferation markers. RESULTS: EPO treatment resulted in splenomegaly, spleen microstructure disorder, splenic corpuscular atrophy, indistinct germinal center, and unclear boundary between white and red pulp structures. RNA-Seq showed that EPO treatment suppressed gene expression associated with immune responses, while promoted cell cycle and DNA replication. IHF and FCM validated that, at the cellular level, T, B, M1 cells were significantly reduced, and M2 cells were significantly decreased after EPO treatment. The proliferation analysis showed that the portion of EDU(+) or Ki-67(+)cells consisted of granulocytes and macrophages, and after EPO treatment, only macrophages showed a significant increase in their number and proportion, while granulocytes did not show a significant response to EPO stimulation. CONCLUSION: Long term high-dose EPO treatment may lead to splenomegaly and immunosuppression of the local immune microenvironment in mice. The mechanism may be related to the increased anti-inflammatory and immunomodulatory functions caused by M2 cells. The study provides, for the first time, the transcriptomic characteristics and immunological of the spleens of EPO treated mice, providing a new perspective for the study of the effects of EPO on mice.
作者：
Ye, Yongjun;Zong, Yifan;Li, Mengyi;Song, Bangzhi
期刊：
Journal of Radioanalytical and Nuclear Chemistry,2025年334(3):2195-2202 ISSN：0236-5731
通讯作者：
Ye, YJ
作者机构：
[Li, Mengyi; Ye, Yongjun; Song, Bangzhi; Zong, Yifan] Univ South China, Sch Resource Environm & Safety Engn, Hengyang 421001, Peoples R China.;[Ye, Yongjun] Univ South China, Key Discipline Lab Natl Def Biotechnol Uranium Min, Hengyang 421001, Peoples R China.
通讯机构：
[Ye, YJ ] U;Univ South China, Sch Resource Environm & Safety Engn, Hengyang 421001, Peoples R China.;Univ South China, Key Discipline Lab Natl Def Biotechnol Uranium Min, Hengyang 421001, Peoples R China.
关键词：
Uranium tailings;The free radon production rate;Temperature;Water-solid mass ratio;Particle size
摘要：
The effect of conventional factors on the free radon production rate above 0 degrees C has been widely studied, but rarely explored under frozen conditions. In order to investigate the effect on the free radon production rate of uranium tailings under frozen and non-frozen conditions, uranium tailings from southern China were selected for screening, and temperature, water-solid mass ratio and particle size were used as influencing factors for research. The stable radon concentration and the free radon production rate of uranium tailings of varying particle sizes at different temperatures (20 degrees C, 0 degrees C, - 10 degrees C, - 20 degrees C) and different water-solid mass ratios (0, 0.14, 0.28) were measured by the homemade radon collection tanks. The experimental results showed that: (1) The free radon production rate decreases with temperature decreases, more significantly under frozen conditions, dropping 3.49-4.16% per 1 degrees C on average. (2) Under non-frozen conditions, the free radon production rate rises with water-solid mass ratio rises, while under frozen conditions, the free radon production rate of uranium tailings first increases and then decreases with the increase of water-solid mass ratio. (3) The larger particle size, the lower the free radon production rate. The free radon production rate of uranium tailings with particle size > 450 mu m is 21.3-81.1% lower than that of uranium tailings with other particle sizes.
作者机构：
[Liu, Xing; Xiao, Xilin; Xiao, XL; Liu, Zhen; Sun, Jie] Univ South China, Sch Publ Hlth, Hengyang Sch Med, Hengyang 421001, Peoples R China.;[Xiao, Xilin; Xiao, XL] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;[Xiao, Xilin; Xiao, XL] Univ South China, Dept Publ Hlth Lab Sci, Hengyang 421001, Peoples R China.
通讯机构：
[Xiao, XL ] U;Univ South China, Sch Publ Hlth, Hengyang Sch Med, Hengyang 421001, Peoples R China.;Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;Univ South China, Dept Publ Hlth Lab Sci, Hengyang 421001, Peoples R China.
关键词：
fluorescent sensor;mercury ion detection;silver ion detection;cascade isothermal signal amplification;catalytic hairpin assembly
摘要：
In this study, novel fluorescent DNA biosensors for mercury (Hg2+) and silver (Ag+) ions were developed based on thymine (T)- and cytosine (C)-rich recognition elements in combination with exonuclease III and a mismatch-catalyzed hairpin assembly (MCHA)-based cascade isothermal signal-amplification strategy. In the presence of the respective target analytes, the recognition element terminals form so-called T-Hg2+-T or C-Ag+-C structures, resulting in cleavage by Exo III and the release of the trigger strand for MCHA. This binds to the H1 hairpin, which is fluorescently labeled with carboxyfluorescein (FAM) and tetramethylrhodamine (TAMRA), disrupting fluorescence resonance energy transfer between them and, thus, restoring FAM fluorescence, generating a strong signal at 520 nm. The linear range of the Hg2+ sensor is 0.5 to 3 pM, with a detection limit of 0.07 pM. The recovery range in actual spiked water samples is between 98.5% and 105.2%, with a relative standard deviation (RSD) ranging from 2.0% to 4.2%. The linear range of the Ag+ sensor is 10 to 90 pM, with a detection limit of 7.6 pM. The recovery range in actual spiked water samples is between 96.2% and 104.1%, with an RSD ranging from 3.2% to 6.3%. The cascade isothermal signal amplification strategy effectively enhances sensor sensitivity, while MCHA decreases the false-positive rate. The aptamer sensor exhibits high specificity, is resistant to interference, and can be used for the detection of Hg2+ and Ag+ in environmental water samples.
期刊：
Reliability Engineering & System Safety,2025年256:110794 ISSN：0951-8320
通讯作者：
Zeng, WJ
作者机构：
[Li, Zheng; Chen, Chuqi; Li, Xiaoyu; Wang, Linna; Zeng, Wenjie; Zeng, WJ] Univ South China, Sch Nucl Sci & Technol, Hengyang City 421001, Peoples R China.
通讯机构：
[Zeng, WJ ] U;Univ South China, Sch Nucl Sci & Technol, Hengyang City 421001, Peoples R China.
关键词：
Small pressurized water reactor;Nuclear power plant;Coordinated control system;Uncertainty quantification;Sensitivity analysis
摘要：
Parameter uncertainty is an inherent characteristic of control systems. To assess the impact of parameter uncertainty on the system, the dynamic performance assessment with uncertainty quantification and sensitivity analysis is proposed. The input sample set is obtained by Wilks' method and Latin hypercube sampling, then dynamic simulation and statistics are used to realize the uncertainty quantification of output parameters and the identification of key output parameters. The central composite design and second-order polynomial are used to build the agent model for the Sobol's method. Taking the small pressurized water reactor nuclear power plant as an example, a performance assessment platform of coordinated control system is developed through graphical user interface with fast load decrease conditions. The study shows that all the transient safety parameters except reactor core relative power and secondary-side outlet temperature of once-through steam generator (OTSG) satisfy the technical requirements, and the control system has a certain safety margin. Meanwhile, the adjustment time of reactor core relative power and secondary-side outlet temperature of OTSG is highly sensitive to primary-side flowrate of OTSG under the small range of variable load, and sensitive to the opening pressure of the steam dump valve under the large range of variable load.
Parameter uncertainty is an inherent characteristic of control systems. To assess the impact of parameter uncertainty on the system, the dynamic performance assessment with uncertainty quantification and sensitivity analysis is proposed. The input sample set is obtained by Wilks' method and Latin hypercube sampling, then dynamic simulation and statistics are used to realize the uncertainty quantification of output parameters and the identification of key output parameters. The central composite design and second-order polynomial are used to build the agent model for the Sobol's method. Taking the small pressurized water reactor nuclear power plant as an example, a performance assessment platform of coordinated control system is developed through graphical user interface with fast load decrease conditions. The study shows that all the transient safety parameters except reactor core relative power and secondary-side outlet temperature of once-through steam generator (OTSG) satisfy the technical requirements, and the control system has a certain safety margin. Meanwhile, the adjustment time of reactor core relative power and secondary-side outlet temperature of OTSG is highly sensitive to primary-side flowrate of OTSG under the small range of variable load, and sensitive to the opening pressure of the steam dump valve under the large range of variable load.
期刊：
INTERDISCIPLINARY SCIENCES-COMPUTATIONAL LIFE SCIENCES,2025年:1-11 ISSN：1913-2751
通讯作者：
Yan, SY
作者机构：
[Yan, Shiyu; Chen, Lingna; Yan, SY; Yu, Gang] Univ South China, Comp Sch, Hengyang 421001, Peoples R China.;[Yang, Jiaoxing] Univ South China, Affiliated Hosp 1, Hengyang 421001, Peoples R China.
通讯机构：
[Yan, SY ] U;Univ South China, Comp Sch, Hengyang 421001, Peoples R China.
关键词：
Deep Learning technique;Synergistic drug combination;Computational model;Feature extraction
摘要：
Drug combination therapy has shown improved efficacy and decreased adverse effects, making it a practical approach for conditions like cancer. However, discovering all potential synergistic drug combinations requires extensive experimentation, which can be challenging. Recent research utilizing deep learning techniques has shown promise in reducing the number of experiments and overall workload by predicting synergistic drug combinations. Therefore, developing reliable and effective computational methods for predicting these combinations is essential. This paper proposed a novel method called Drug-molecule Connect Cell (DconnC) for predicting synergistic drug combinations. DconnC leverages cellular features as nodes to establish connections between drug molecular structures, allowing the extraction of pertinent features. These features are then optimized through self-augmented contrastive learning using bidirectional recurrent neural networks (Bi-RNN) and long short-term memory (LSTM) models, ultimately predicting the drug synergy. By integrating information about the molecular structure of drugs for the extraction of cell features, DconnC uncovers the inherent connection between drug molecular structures and cellular characteristics, thus improving the accuracy of predictions. The performance of our method is evaluated using a five-fold cross validation approach, demonstrating a 35
$$\%$$
reduction in the mean square error (MSE) compared to the next-best method. Moreover, our method significantly outperformed alternative approaches in various evaluation criteria, particularly in predicting different cell lines and Loewe synergy score intervals.
作者机构：
[Huang, Wenhua; Zhang, Jiankai; Zhan, Jianan; Zhang, JK] Guangdong Med Univ, Sch Basic Med Sci, Dept Human Anat, Zhanjiang 524000, Peoples R China.;[Wang, YL; Wang, Yilin] China Med Univ, Coll Basic Med Sci, Dept Human Anat, Shenyang 110122, Peoples R China.;[Huang, Wenhua; Chen, Qiwei; Zhou, Xi; Zhang, Xianlin] Southern Med Univ, Sch Basic Med Sci, Guangdong Prov Key Lab Med Biomech, Natl Key Discipline Human Anat, Guangzhou 510515, Peoples R China.;[Huang, Wenhua; Kong, Yueying] Univ South China, Clin Anat & Reprod Med Applicat Inst, Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Gong, Haihuan] Guangzhou Med Univ, Affiliated Stomatol Hosp, Guangzhou Key Lab Basic & Appl Res Oral Regenerat, Dept Periodont, Guangzhou 510182, Peoples R China.
通讯机构：
[Huang, WH ; Zhang, JK] G;[Wang, YL ] C;Guangdong Med Univ, Sch Basic Med Sci, Dept Human Anat, Zhanjiang 524000, Peoples R China.;China Med Univ, Coll Basic Med Sci, Dept Human Anat, Shenyang 110122, Peoples R China.;Southern Med Univ, Sch Basic Med Sci, Guangdong Prov Key Lab Med Biomech, Natl Key Discipline Human Anat, Guangzhou 510515, Peoples R China.
摘要：
Myoelectric biofeedback (EMG-BF) is a widely recognized and effective method for treating movement disorders caused by impaired nerve function. However, existing EMG-feedback devices are almost entirely located in large medical centers, which greatly limits patient accessibility. To address this critical limitation, there is an urgent need to develop a portable, cost-effective, and real-time monitoring device that can transcend the existing barriers to the treatment of EMG-BF. Our proposed solution leverages polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP) as core materials, ingeniously incorporating wood pulp nano celluloses (CNF-P)-Na(+) to enhance the structural integrity. Additionally, the inclusion of nano-silica particles further augments the sensor's capabilities, enabling the creation of a stress-sensitive mineral ionization hydrogel sensor. This innovative approach not only capitalizes on the superior rheological properties of the materials but also, through advanced 3D printing technology, facilitates the production of a micro-scale structural hydrogel sensor with unparalleled sensitivity, stability, and durability. The potential of this sensor in the realm of human motion detection is nothing short of extraordinary. This development can potentially improve the treatment landscape for EMG-BF offering patients more convenient and efficient therapeutic options.
摘要：
In uranium(VI) (U(VI)) photoreduction, it is still a challenge to simultaneously degrade naturally coexisting organics and explore their impact on U(VI) photoreduction. Meanwhile, how to boost the separation and transport capability of photo-induced carriers is also a hot topic of current research. In this work, an ultrathin Bi 2 WO 6 /Bi 2 MoO 6 Z-type heterojunction was developed for the simultaneous treatment of U(VI) and its co-existing organics (tannic acid (TA)). Ultrathin interface engineering increased the contact area and shortened the transmission distance of photo-induced carriers. As a result, ultrathin Bi 2 WO 6 /Bi 2 MoO 6 exhibited highly efficient U(VI) removal rate (95.8 %) and TA degradation rate (97.2 %), which were much higher than those of bulk Bi 2 WO 6 /Bi 2 MoO 6 and single ultrathin Bi 2 WO 6 . In addition, the material possessed excellent stability and recyclability. Importantly, TA not only enhanced U(VI) removal by eliminating holes, but also favored U(VI) removal by serving as a bridge to the catalyst for U(VI) adsorption. Finally, a new mechanism was proposed.
In uranium(VI) (U(VI)) photoreduction, it is still a challenge to simultaneously degrade naturally coexisting organics and explore their impact on U(VI) photoreduction. Meanwhile, how to boost the separation and transport capability of photo-induced carriers is also a hot topic of current research. In this work, an ultrathin Bi 2 WO 6 /Bi 2 MoO 6 Z-type heterojunction was developed for the simultaneous treatment of U(VI) and its co-existing organics (tannic acid (TA)). Ultrathin interface engineering increased the contact area and shortened the transmission distance of photo-induced carriers. As a result, ultrathin Bi 2 WO 6 /Bi 2 MoO 6 exhibited highly efficient U(VI) removal rate (95.8 %) and TA degradation rate (97.2 %), which were much higher than those of bulk Bi 2 WO 6 /Bi 2 MoO 6 and single ultrathin Bi 2 WO 6 . In addition, the material possessed excellent stability and recyclability. Importantly, TA not only enhanced U(VI) removal by eliminating holes, but also favored U(VI) removal by serving as a bridge to the catalyst for U(VI) adsorption. Finally, a new mechanism was proposed.
摘要：
BACKGROUND AND OBJECTIVE: Alpha-ketoglutarate (AKG), is a major intermediate metabolite of the tricarboxylic acid cycle, and is closely associated with cardiometabolic disease prognosis. Previous studies indicated that AKG is related to myocardial energy expenditure levels and reflects adverse short-term outcomes in heart failure (HF) patients. In this prospective cohort study, we examined the long-term prognostic value of AKG levels in acute HF (AHF) patients. METHODS: Plasma AKG levels were assessed in patients hospitalized with AHF. Hazard ratios (HRs) and 95% confidence intervals (CIs) for all-cause mortality were calculated via multiple Cox regression. All-cause mortality was compared between patients with NT-proBNP < 1000 pg/ml and those with NT-proBNP ≥ 1000 pg/ml via subgroup analysis. RESULTS: Patients with AKG ≥ 9.83 μg/ml had higher heart rates and NT-proBNP and lower left ventricular ejection fraction (LVEF) and systolic blood pressure (SBP). After multiple adjustment, higher AKG was associated with an increased all-cause mortality risk (HR = 1.078, p < 0.001). Compared with AKG < 9.83 μg/ml, AKG ≥ 9.83 μg/ml nearly doubled (HR = 1.929, p < 0.001) and quadrupled (HR = 4.160, p < 0.001) the all-cause mortality risk in patients with NT-proBNP ≥ 1000 pg/ml and those with NT-proBNP < 1000 pg/ml, respectively. CONCLUSIONS AND RELEVANCE: Plasma AKG was independently associated with greater all-cause mortality risk in patients with AHF. Higher AKG levels retained prognostic value for patients with relatively low NT-proBNP.
期刊：
Journal of the Franklin Institute,2025年362(1):107397 ISSN：0016-0032
通讯作者：
Wang, H
作者机构：
[Nie, Liang] Univ South China, Sch Elect Engn, Hengyang 421001, Peoples R China.;[Wang, Hui] Liaocheng Univ, Sch Math Sci, Liaocheng 252000, Peoples R China.;[Sun, Yichong] Chinese Univ Hong Kong, Multiscale Med Robot Ctr, Hong Kong 999077, Peoples R China.
通讯机构：
[Wang, H ] L;Liaocheng Univ, Sch Math Sci, Liaocheng 252000, Peoples R China.
摘要：
In this paper, a switched linear parameter-varying (LPV) resilient tracking controller is designed for rigid-body under actuator faults, uncertainties in measurement of scheduling parameters and time-delay in detection of system modes. The nonlinear attitude dynamics of rigid-body is constructed as a switched LPV system in which persistent dwell-time switching rule is used to regulate the switches caused by abrupt and intermittent actuator failures. Thereafter, by constructing a class of both parameter-dependent and time-dependent multiple Lyapunov functions (MLFs), a switched LPV resilient tracking controller is developed in order that the global uniform exponential stability and desired L∞ performance of the underlying system are achieved even with uncertain scheduling parameters, mismatched modes and persistent external disturbances. Furthermore, the nonconvex conditions of control synthesis are converted into parameterized linear matrix inequalities that can be readily resolved via gridding technique. Finally, the availability of the provided approach is evaluated with a numerical simulation.
In this paper, a switched linear parameter-varying (LPV) resilient tracking controller is designed for rigid-body under actuator faults, uncertainties in measurement of scheduling parameters and time-delay in detection of system modes. The nonlinear attitude dynamics of rigid-body is constructed as a switched LPV system in which persistent dwell-time switching rule is used to regulate the switches caused by abrupt and intermittent actuator failures. Thereafter, by constructing a class of both parameter-dependent and time-dependent multiple Lyapunov functions (MLFs), a switched LPV resilient tracking controller is developed in order that the global uniform exponential stability and desired L∞ performance of the underlying system are achieved even with uncertain scheduling parameters, mismatched modes and persistent external disturbances. Furthermore, the nonconvex conditions of control synthesis are converted into parameterized linear matrix inequalities that can be readily resolved via gridding technique. Finally, the availability of the provided approach is evaluated with a numerical simulation.
通讯机构：
[Li, YH ] U;[Jiang, YJ ] C;Cent Hosp Yongzhou, Dept Oncol, Yongzhou 425000, Hunan, Peoples R China.;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Oncol, 69 Chuanshan Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
bioinformatics;biomarker;predictive factor;ribophorin family 1;tumor microenvironment
摘要：
The ribophorin family, including RPN1, has been associated with tumor progression, but its specific role in pan-cancer dynamics remains unclear. Using data from TCGA, GTEx, and Ualcan databases, we investigated the relationship of RPN1 with prognosis, genomic alterations, and epigenetic modifications across various cancers. Differential analysis revealed elevated RPN1 expression in multiple cancer types, indicating a potential prognostic value. Amplification was the predominant mutation type of RPN1 in pan-cancer, with notable correlations with DNA methylation and copy number variation. Gene set variation analysis identified RPN1's involvement in cancer development, immunity, and metabolism. Additionally, RPN1 expression correlated with the tumor microenvironment, immune response factors, and response to anti-tumor therapies. Functional validation in triple-negative breast cancer, glioblastoma, and bladder cancer cell lines demonstrated the role of RPN1 in tumor cell proliferation and migration. Our findings highlight RPN1 as a potential biomarker for cancer diagnosis and treatment response in pan-cancer therapy.
作者机构：
[Fan, Chenzhou; Wang, Tianlin] Univ South China, Sch Civil Engn, Hengyang 421001, Peoples R China.;[Xu, Feng; Xu, F; Guo, Changqing] Univ South China, Sch Math & Phys, Hengyang 421001, Peoples R China.
通讯机构：
[Xu, F ] U;Univ South China, Sch Math & Phys, Hengyang 421001, Peoples R China.
关键词：
Foundation excitation;Gap constraint;Fluid-conveying pipe;Impact vibration
摘要：
In practical engineering, the unilateral gap constraint impact vibration in fluid-conveying pipes is a critical concern, often arising from constraint loosening or the presence of barriers around the pipe. These factors can significantly affect the service life and safety reliability of the pipes. A tension and compression anisotropy spring with rapidly increasing restoring force in compression but almost zero restoring force in tension is proposed to simulate the unilateral gap constraint, model a fluid-conveying pipe with a Pfluger column. Its reliability is also verified based on the bifurcation phase diagram comparison between the small and large stiffness conditions. Subsequently, we establish a vibration mechanics analysis model for the cantilever fluid-conveying pipe under foundation excitation to assess the effects of basic excitation frequency, fluid velocity, distributed follower force, position coordinate of unilateral gap constraint, and the viscoelastic coefficient on the impact vibration stability of the fluid-conveying pipe. Our findings reveal that when the period-doubling bifurcation sequence is interrupted by Bare-grazing bifurcation, the system directly transitions into chaotic vibration or induces a new period-doubling bifurcation sequence, followed by re-entry into chaotic vibration. These results provide valuable insights into the intricate dynamics of fluid-conveying pipes under foundation excitation, offering a deeper understanding of the impact of various parameters on the pipe's vibration.
In practical engineering, the unilateral gap constraint impact vibration in fluid-conveying pipes is a critical concern, often arising from constraint loosening or the presence of barriers around the pipe. These factors can significantly affect the service life and safety reliability of the pipes. A tension and compression anisotropy spring with rapidly increasing restoring force in compression but almost zero restoring force in tension is proposed to simulate the unilateral gap constraint, model a fluid-conveying pipe with a Pfluger column. Its reliability is also verified based on the bifurcation phase diagram comparison between the small and large stiffness conditions. Subsequently, we establish a vibration mechanics analysis model for the cantilever fluid-conveying pipe under foundation excitation to assess the effects of basic excitation frequency, fluid velocity, distributed follower force, position coordinate of unilateral gap constraint, and the viscoelastic coefficient on the impact vibration stability of the fluid-conveying pipe. Our findings reveal that when the period-doubling bifurcation sequence is interrupted by Bare-grazing bifurcation, the system directly transitions into chaotic vibration or induces a new period-doubling bifurcation sequence, followed by re-entry into chaotic vibration. These results provide valuable insights into the intricate dynamics of fluid-conveying pipes under foundation excitation, offering a deeper understanding of the impact of various parameters on the pipe's vibration.
摘要：
Aim To explore and explain the mechanisms that influence surface acting in nursing students with different characteristics.
To explore and explain the mechanisms that influence surface acting in nursing students with different characteristics.
Background Nurses are now expected to deliver patient-centered care which necessitates the emotional labor. Surface acting, a form of emotional labor, can lead to negative outcomes. Given that nursing students are the backbone of the future nursing profession, there is an urgent need to investigate their surface acting tendencies and identify potential factors for early intervention.
Nurses are now expected to deliver patient-centered care which necessitates the emotional labor. Surface acting, a form of emotional labor, can lead to negative outcomes. Given that nursing students are the backbone of the future nursing profession, there is an urgent need to investigate their surface acting tendencies and identify potential factors for early intervention.
Design A cross-sectional study.
A cross-sectional study.
Methods This study was surveyed in a vocational college in Gansu, China. Participants completed the general information questionnaire, Bem Sex Role Inventory, Professional Identity Questionnaire of Nursing Students and Surface Acting Scale. K-means cluster analysis was performed, followed by random forest algorithm and SHapley Additive exPlanations based on Python program.
This study was surveyed in a vocational college in Gansu, China. Participants completed the general information questionnaire, Bem Sex Role Inventory, Professional Identity Questionnaire of Nursing Students and Surface Acting Scale. K-means cluster analysis was performed, followed by random forest algorithm and SHapley Additive exPlanations based on Python program.
Results A total of 1241 nursing students from vocational college were investigated and were clustered into 4 groups. The five dimensions of professional identity had higher feature importance in all four groups, with professional self-image having the highest feature importance in Cluster 3. Professional self-image and understanding retention benefits and turnover risks were negative predictors of surface acting in all four groups. Social comparison and self-reflection, independence of career choice and social modeling regarding nursing profession were positively correlated with surface acting in specific groups. In Cluster 1, there exists a positive correlation between professional self-image and the constructs of social comparison and self-reflection; as well as a negative correlation between maternal education and understanding of retention benefits and turnover risks.
A total of 1241 nursing students from vocational college were investigated and were clustered into 4 groups. The five dimensions of professional identity had higher feature importance in all four groups, with professional self-image having the highest feature importance in Cluster 3. Professional self-image and understanding retention benefits and turnover risks were negative predictors of surface acting in all four groups. Social comparison and self-reflection, independence of career choice and social modeling regarding nursing profession were positively correlated with surface acting in specific groups. In Cluster 1, there exists a positive correlation between professional self-image and the constructs of social comparison and self-reflection; as well as a negative correlation between maternal education and understanding of retention benefits and turnover risks.
Conclusions Professional identity significantly influences surface acting behaviors among nursing students, with professional self-image serving as a key negative predictor. Positive family conditions, access to educational resources, parental literacy, masculine or feminine gender roles and first-year nursing students, these traits have implications when dimensions of professional identity are used to predict surface acting behaviors.
Professional identity significantly influences surface acting behaviors among nursing students, with professional self-image serving as a key negative predictor. Positive family conditions, access to educational resources, parental literacy, masculine or feminine gender roles and first-year nursing students, these traits have implications when dimensions of professional identity are used to predict surface acting behaviors.
通讯机构：
[Zeng, QY ; Xiao, Y ; Zhang, QS] U;Univ South China, Sch Resource & Environm & Safety Engn, Hengyang 421001, Peoples R China.;Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.
摘要：
Photocatalytic extraction of uranium from solution is one of the most promising technologies for uranium recovery. However, the presence of axial bonds between the two oxygen atoms in UO 2 2+ creates a high activation barrier for electron transfer, which hinders the reduction of UO 2 2+ , making it more challenging to reduce U( VI ) to U( IV ) with conventional photocatalysis. Herein, an ingenious strategy for the efficient removal of uranium from aqueous solutions, utilizing the photocatalytic hydrogen evolution reaction (HER) process, is proposed. In this process, a novel and highly stable bimetallic Co 9 Ni 1 -HOF was employed as a high HER performance photocatalyst, with an overpotential of 355 mV at 10 mA cm −2 . Benefiting from the excellent HER activity of Co 9 Ni 1 -HOF, efficient uranium removal (∼95.6%) within 60 min of illumination was achieved. The superior uranium removal performance could be ascribed to the enhanced HER performance due to the incorporation of Ni and the high affinity between U and OH*, resulting from the electron transfer between U and O and N, as confirmed by a series of experiments and density functional theory calculations. This work provides a new approach for the efficient removal of uranium using photocatalytic HER performance.
摘要：
Predicting the binding affinity of drug target is essential to reduce drug development costs and cycles. Recently, several deep learning-based methods have been proposed to utilize the structural or sequential information of drugs and targets to predict the drug-target binding affinity (DTA). However, methods that rely solely on sequence features do not consider hydrogen atom data, which may result in information loss. Graph-based methods may contain information that is not directly related to the prediction process. Additionally, the lack of structured division can limit the representation of characteristics. To address these issues, we propose a multimodal DTA prediction model using graph local substructures, called MLSDTA. This model comprehensively integrates the graph and sequence modal information from drugs and targets, achieving multimodal fusion through a cross-attention approach for multimodal features. Additionally, adaptive structure aware pooling is applied to generate graphs containing local substructural information. The model also utilizes the DropNode strategy to enhance the distinctions between different molecules. Experiments on two benchmark datasets have shown that MLSDTA outperforms current state-of-the-art models, demonstrating the feasibility of MLSDTA.
摘要：
Synovial inflammation plays a crucial role in osteoarthritis (OA) development, leading to chronic inflammation and cartilage destruction. Although targeting synovitis can alleviate OA, clinical outcomes have been disappointing due to poor drug targeting and joint cavity heterogeneity. This study presents pH-responsive lipid nanoparticles (LNPs@UA), loaded with Urolithin A (UA), as a potential OA treatment. LNPs@UA showed uniform particle size, low zeta potential, and effective mitochondria-targeting and pH-responsive capabilities. In vitro, LNPs@UA reduced reactive oxygen species (ROS), pro-inflammatory factors (IL-1β, IL-6, TNF-α), and promoted M2 macrophage polarization. It improved mitochondrial structure, enhanced autophagy, and inhibited ferroptosis. In vivo, LNPs@UA alleviated OA progression in an ACLT-induced OA mouse model. Transcriptomic analysis revealed inhibition of NF-κB signaling and activation of repair pathways. These results suggest LNPs@UA could offer a promising therapeutic approach for OA.
Synovial inflammation plays a crucial role in osteoarthritis (OA) development, leading to chronic inflammation and cartilage destruction. Although targeting synovitis can alleviate OA, clinical outcomes have been disappointing due to poor drug targeting and joint cavity heterogeneity. This study presents pH-responsive lipid nanoparticles (LNPs@UA), loaded with Urolithin A (UA), as a potential OA treatment. LNPs@UA showed uniform particle size, low zeta potential, and effective mitochondria-targeting and pH-responsive capabilities. In vitro, LNPs@UA reduced reactive oxygen species (ROS), pro-inflammatory factors (IL-1β, IL-6, TNF-α), and promoted M2 macrophage polarization. It improved mitochondrial structure, enhanced autophagy, and inhibited ferroptosis. In vivo, LNPs@UA alleviated OA progression in an ACLT-induced OA mouse model. Transcriptomic analysis revealed inhibition of NF-κB signaling and activation of repair pathways. These results suggest LNPs@UA could offer a promising therapeutic approach for OA.
摘要：
Sepsis-associated acute respiratory distress syndrome (ARDS) is a heterogeneous disease with high morbidity and mortality. Lactylation plays a crucial role in sepsis and sepsis-induced lung injury. This study aimed to identify distinct lactylation-based phenotypes in patients with sepsis-associated ARDS and determine relevant molecular biomarkers. We analyzed blood transcriptome and clinical data from patients with sepsis-associated ARDS and calculated the lactylation activity. KEGG pathway analysis, drug sensitivity prediction, and immune cell infiltration analysis were performed. Candidate molecular biomarkers were identified by intersecting the feature genes extracted from four machine learning models. Lactylation activity showed significant heterogeneity among patients with sepsis-associated ARDS, which enabled the classification into low- and high-lactylation activity phenotypes. Patients with high-lactylation experienced longer hospital stays and higher mortality rates, as well as distinct signaling pathways, drug responses, and circulating immune cell abundances. Six key markers (ALDOB, CCT5, EP300, PFKP, PPIA, and SIRT1) were identified to differentiate the two lactylation activity phenotypes, all significantly correlated with circulating immune cell populations. This study revealed significant heterogeneity in lactylation activity phenotypes among patients with sepsis-associated ARDS and identified potential biomarkers to facilitate the application of these phenotypes in clinical practice.
作者机构：
[Zhong, Jing; Zhao, Hu; Zhong, J; Xiao, Qian] Univ South China, Affiliated Hosp 1, Canc Res Inst, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Qian] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Clin Lab Med, Hengyang 421001, Hunan, Peoples R China.;[An, Yangfang] Yiyang Cent Hosp, Yiyang 413099, Hunan, Peoples R China.;[Wang, Mu] Univ South China, Affiliated Nanhua Hosp, Clin Res Inst, Clin Mass Spectrometry Lab, Hengyang, Peoples R China.;[Zhong, Jing] Univ South China, Affiliated Hosp 1, Inst Clin Med, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Zhong, J ; Wang, M ] U;Univ South China, Affiliated Hosp 1, Canc Res Inst, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Nanhua Hosp, Clin Res Inst, Clin Mass Spectrometry Lab, Hengyang, Peoples R China.
摘要：
Phospholipids, complex lipids prevalent in the human body, play crucial roles in various pathophysiological processes. Beyond their synthesis and degradation, phospholipids can influence chemoresistance by participating in ferroptosis. Extensive evidence highlights the significant link between tumor drug resistance and phospholipids. Therefore, drugs targeting phospholipid metabolism itself or the synthesis of corresponding composite materials will effectively overcome the difficulties of clinical tumor treatment.
Phospholipids, complex lipids prevalent in the human body, play crucial roles in various pathophysiological processes. Beyond their synthesis and degradation, phospholipids can influence chemoresistance by participating in ferroptosis. Extensive evidence highlights the significant link between tumor drug resistance and phospholipids. Therefore, drugs targeting phospholipid metabolism itself or the synthesis of corresponding composite materials will effectively overcome the difficulties of clinical tumor treatment.
