摘要：
Background: Triple-negative breast cancer (TNBC) is a subtype of BC with high rates of mortality. The mechanism of PTPRG-AS1 in ferroptosis of TNBC was investigated. Methods: Chromatin immunoprecipitation and dual-luciferase reporter assays were used to measure intermolecular relationships. MTT and colony formation assays detected cell viability and proliferation. Kits detected Fe2+ and reactive oxygen species levels. The role of PTPRG-AS1 in tumor growth was analyzed in vivo. Results: PTPRG-AS1 was increased in TNBC tissues and cells. PTPRG-AS1 silencing increased the reduction of glutathione and GPX4, increased Fe2+ and reactive oxygen species in erastin-treated cells and inhibited proliferation. POU2F2 transcriptionally upregulated PTPRG-AS1. PTPRG-AS1 targeted miR-376c-3p to upregulate SLC7A11. PTPRG-AS1 knockdown suppressed tumor growth in vivo. Conclusion: POU2F2 transcriptionally activates PTPRG-AS1 to modulate ferroptosis and proliferation by miR-376c-3p/SLC7A11, promoting TNBC. Tweetable abstract: Triple-negative breast cancer is the most malignant subtype of BC. POU2F2 transcriptionally upregulated PTPRG-AS1. PTPRG-AS1 targeted miR-376c-3p to increase SLC7A11 expression. POU2F2 upregulated PTPRG-AS1 to inhibit ferroptosis in triple-negative breast cancer by miR-376c-3p/SLC7A11. Plain language summary: Triple-negative breast cancer (TNBC) is a kind of breast cancer with high recurrence and low survival rates. Activation of the ferroptosis pathway can inhibit BC proliferation and distant metastasis. Therefore, identifying effective biomarkers and molecular mechanisms of ferroptosis in TNBC is important for its earlier detection and therapy. PTPRG-AS1 is a new type of lncRNA discovered in recent years that is increased in various diseases and is related to prognosis. In the present study, the authors found that POU2F2 promoted PTPRG-AS1 transcription. PTPRG-AS1 knockdown activated ferroptosis in TNBC and inhibited proliferation. Mechanistically, PTPRG-AS1 targeted miR-376c-3p to upregulate SLC7A11, thereby inhibiting ferroptosis and promoting TNBC development. These results indicate that PTPRG-AS1 is a possible therapeutic target in TNBC.
作者机构：
[Feng, Wen-Jie; Wu, Xiao-Ping; Zhu, Hong -Bo; He, Zhi-Long; Tan, Ye-Ru; Xun, Yi; Li, Yue-Hua; Jiang, Yi-Ling; Zhu, HB] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Med Oncol, Hengyang, Hunan, Peoples R China.;[Jiang, Bao-Hong] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Pharm, Hengyang, Hunan, Peoples R China.
通讯机构：
[Zhu, HB ] U;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Med Oncol, Hengyang, Hunan, Peoples R China.
关键词：
AIFM2;GPX4;circRNAs;hepatocellular cancer (HCC);miR-6085
摘要：
BACKGROUND: Circular RNAs (circRNAs) represent a subset of non-coding RNAs implicated in the regulation of diverse biological processes, including tumorigenesis. However, the expression and functional implications of circ0060467 in hepatocellular carcinoma (HCC) remain elusive. In this study, we aimed to elucidate the role of circ0060467 in modulating the progression of HCC. METHODS: Differentially expressed circRNAs in HCC tissues were identified through circRNA microarray assays. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays revealed the upregulation of circ0060467 in both HCC cell lines and tissues. Various assays were conducted to investigate the roles of circ0060467 in HCC progression. Additionally, RNA immunoprecipitation (RIP) assays and luciferase assays were carried out to assess the interactions between circ0060467, microRNA-6085 (miR-6085), apoptosis-inducing factor mitochondria-associated 2 (AIFM2), and glutathione peroxidase 4 (GPX4) in HCC. RESULTS: Microarray and qRT-PCR analyses demonstrated a marked elevation of circ0060467 in HCC tissues and cell lines. Knockdown of circ0060467 suppressed HCC cell proliferation. Luciferase reporter and RIP assays confirmed the binding of circ0060467, AIFM2, and GPX4 to miR-6805. Subsequent experiments revealed that circ0060467 competes with AIFM2 and GPX4, thereby inhibiting cancer cell ferroptosis by binding to miR-6085 and promoting hepatocellular carcinoma progression. CONCLUSIONS: Collectively, circ0060467 modulates the levels of AIFM2 and GPX4, crucial regulators of tumor cell ferroptosis, by acting as a sponge for miR-6085 in HCC. Thus, circ0060467 may represent a novel diagnostic marker and therapeutic target for HCC.
期刊：
Human & Experimental Toxicology,2024年43 ISSN：0960-3271
通讯作者：
Wang, M;Yang, Q
作者机构：
[Ou, Guifang; Liu, Xiaocheng; Qin, Xinru; Liu, Xu; Tang, Yonghong; Rang, Ouyan; Wang, Mu; Yang, Qing] Univ South China, Affiliated Nanhua Hosp, Clin Mass Spectrometry Lab,Nucl Ind Hyg Sch, Clin Res Inst,Hengyang Med Sch, Hengyang, Peoples R China.;[Ou, Guifang; Liu, Xiaocheng; Qin, Xinru; Liu, Xu; Tang, Yonghong; Rang, Ouyan; Wang, Mu; Yang, Qing] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Basic Med,Nucl Ind Hyg Sch, Hengyang, Peoples R China.;[Qin, Xinru; Liu, Xu] Univ South China, Sch Publ Hlth, Hengyang, Peoples R China.;[Li, Guojuan] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Endocrinol Dept, Hengyang, Peoples R China.;[Wang, Mu] Univ South China, Affiliated Nanhua Hosp, Clin Mass Spectrometry Lab,Clin Res Inst, Hengyang Med Sch,Nucl Ind Hyg Sch, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Wang, M ; Yang, Q ] U;Univ South China, Affiliated Nanhua Hosp, Clin Mass Spectrometry Lab,Clin Res Inst, Hengyang Med Sch,Nucl Ind Hyg Sch, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Nanhua Hosp, Dept Basic Med,Nucl Ind Hyg Sch, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dongfeng Nan Rd 336, Hengyang, Peoples R China.
摘要：
Human & Experimental Toxicology, Volume 43, Issue , January-December 2024. <br/>Adipose tissue is the main organ that stores lipids and it plays important roles in metabolic balance in the body. We recently reported in Human and Experimental Toxicology that the combined exposure to BPA and fructose may interfere with energy metabolism of adipose tissue. However, it is still unclear whether the combined exposure to BPA and fructose has the possibility to induce lipid remodeling in adipose tissue. In the present study, we performed a widely targeted quantitative lipidomic analysis of the adipose tissue of rats after 6 months of BPA and fructose combined exposure. We totally determined 734 lipid molecules in the adipose tissue of rats. Principal component analysis (PCA) showed the group of the combined exposure to higher-dose (25 μg/kg every other day) BPA and fructose can be distinguished from the groups of control, higher-dose BPA exposure and fructose exposure clearly. Partial least squares-discriminant analysis (PLS-DA) and univariate statistical analysis displayed lipids of PC(18:0_ 20:3), TG(8:0_14:0_16:0), TG(12:0_14:0_16:1), TG(10:0_16:0_16:1), TG(12:0_ 14:0_18:1), TG(14:0_ 16:0_16:1), TG(14:0_14:1_16:1), TG(8:0_ 16:1_16:2), TG(14:1_16:1_ 16:1), TG(16:1_18:1_18:1), TG(16:0_16:1_20:4) and TG(15:0_18:1_ 24:1) may contributed the most to the discrimination. These findings indicated that combined exposure to BPA and fructose has the potential to cause lipid remodeling in adipose tissue.
摘要：
The GECAM series of satellites utilizes LaBr
$$_3$$
(Ce), LaBr
$$_3$$
(Ce,Sr), and NaI(Tl) crystals as sensitive materials for gamma-ray detectors (GRDs). To investigate the nonlinearity in the detection of low-energy gamma rays and address the errors in the calibration of the E–C relationship, comprehensive tests and comparative studies of the three aforementioned crystals were conducted using Compton electrons, radioactive sources, and mono-energetic X-rays. The nonlinearity test results of the Compton electrons and X-rays demonstrated substantial differences, with all three crystals presenting a higher nonlinearity for X/
$$\gamma$$
-rays than for Compton electrons. Despite the LaBr
$$_3$$
(Ce) and LaBr
$$_3$$
(Ce,Sr) crystals having higher absolute light yields, they exhibited a noticeable nonlinear decrease in the light yield, especially at energies below 400 keV. The NaI(Tl) crystal demonstrated an "excess" light output in the 6–200 keV range, reaching a maximum "excess" of 9.2% at 30 keV in the X-ray testing and up to 15.5% at 14 keV during Compton electron testing, indicating a significant advantage in the detection of low-energy gamma rays. Furthermore, we explored the underlying causes of the observed nonlinearity in these crystals. This study not only elucidates the detector responses of GECAM, but also initiates a comprehensive investigation of the nonlinearity of domestically produced lanthanum bromide and sodium iodide crystals.
期刊：
Journal of Surgical Oncology,2024年129(6) ISSN：0022-4790
通讯作者：
Chen, LQ
作者机构：
[Chen, Long-Qi; Yan, Cheng-Yi; Shang, Qi-Xin; Wang, Wen-Ping; Gu, Yi-Min; Yuan, Yong; Zhang, Han-Lu; Yang, Yu-Shang] Sichuan Univ, West China Hosp, Dept Thorac Surg, 37 Guoxue Alley, Chengdu 610041, Sichuan, Peoples R China.;[Yan, Cheng-Yi] Univ South China, Changsha Cent Hosp, Dept Cardiothorac Surg, Changsha, Hunan, Peoples R China.;[Shi, Gui-Dong] North Sichuan Med Coll, Affiliated Hosp, Dept Cardiothorac Surg, Nanchong, Sichuan, Peoples R China.
通讯机构：
[Chen, LQ ] S;Sichuan Univ, West China Hosp, Dept Thorac Surg, 37 Guoxue Alley, Chengdu 610041, Sichuan, Peoples R China.
关键词：
depth of muscularis propria invasion;esophageal squamous cell carcinoma;lymph node metastasis;subclassification;survival
摘要：
BACKGROUND: Whether T2 esophageal squamous cell carcinoma should be subclassified remains controversial. We aimed to investigate the impact of the depth of muscularis propria invasion on nodal status and survival outcomes. METHODS: We identified patients with pT2 esophageal squamous cell carcinoma who underwent primary surgery from January 2009 to June 2017. Clinical data were extracted from prospectively maintained databases. Tumor muscularis propria invasion was stratified into superficial or deep. Binary logistic regression was used to determine risk factors for lymph node metastases. The impact of the depth of muscularis propria invasion on survival was investigated using Kaplan-Meier analysis and a Cox proportional hazard regression model. RESULTS: A total of 750 patients from three institutes were investigated. The depth of muscularis propria invasion (odds ratio [OR]: 3.95, 95% confidence interval [CI]: 2.46-6.35; p < 0.001) was correlated with lymph node metastases using logistic regression. T substage (hazard ratio [HR]: 1.37, 95% CI: 1.05-1.79; p < 0.001) and N status (HR: 1.51, 95% CI: 1.05-2.17; p < 0.001) were independent risk factors in multivariate Cox regression analysis. The deep muscle invasion was associated with worse overall survival (HR: 1.52, 95% CI: 1.19-1.94; p = 0.001) than superficial, specifically in T2N0 patients (HR: 1.38, 95% CI: 1.08-1.94; p = 0.035). CONCLUSIONS: We found that deep muscle invasion was associated with significantly worse outcomes and recommended the substaging of pT2 esophageal squamous cell carcinoma in routine pathological examination.
作者机构：
[Li, Xu; Yuan, Jiaying; Liu, Yujing; Zi, Yuheng; Tang, Yuqing; Liao, Haiyan; Liu, Qinru; Shen, Qin; Tan, Changlian; Cai, Sainan; Wang, Min; Liu, Jun] Cent South Univ, Xiangya Hosp 2, Dept Radiol, Changsha, Peoples R China.;[Liu, Jun; Liao, Haiyan] Clin Res Ctr Med Imaging Hunan Prov, Changsha, Peoples R China.;[Zi, Yuheng] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Radiol, Hengyang, Peoples R China.
通讯机构：
[Zi, YH ; Liao, HY] C;Cent South Univ, Xiangya Hosp 2, Dept Radiol, Changsha, Peoples R China.;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Radiol, Hengyang, Peoples R China.
关键词：
depression;Parkinson's disease;structural magnetic resonance imaging;surface-based morphometry (SBM);voxel-based morphometry (VBM)
摘要：
AimsThe aim of this study is to investigate differences in gray matter volume and cortical complexity between Parkinson's disease with depression (PDD) patients and Parkinson's disease without depression (PDND) patients.MethodsA total of 41 PDND patients, 36 PDD patients, and 38 healthy controls (HC) were recruited and analyzed by Voxel-based morphometry (VBM) and surface-based morphometry (SBM). Differences in gray matter volume and cortical complexity were compared using the one-way analysis of variance (ANOVA) and correlated with the Hamilton Depression Scale-17 (HAMD-17) scores.ResultsPDD patients exhibited significant cortical atrophy in various regions, including bilateral medial parietal-occipital-temporal lobes, right dorsolateral temporal lobes, bilateral parahippocampal gyrus, and bilateral hippocampus, compared to HC and PDND groups. A negative correlation between the GMV of left precuneus and HAMD-17 scores in the PDD group tended to be significant (r = -0.318, p = 0.059). Decreased gyrification index was observed in the bilateral insular and dorsolateral temporal cortex. However, there were no significant differences found in fractal dimension and sulcal depth.ConclusionOur research shows extensive cortical structural changes in the insular cortex, parietal-occipital-temporal lobes, and hippocampal regions in PDD. This provides a morphological perspective for understanding the pathophysiological mechanism underlying depression in Parkinson's disease. The first row of pictures shows extensive cortical volume loss in Parkinson's disease with depression patients, primarily concentrated in the parietal-occipital-temporal lobes, parahippocampal gyrus, and hippocampus. The second row of pictures shows decreased gyrification index in the insula.image
摘要：
The permeability of the blood-brain barrier (BBB) is increased in Alzheimer's disease (AD). This plays a key role in the instigation and maintenance of chronic inflammation during AD. Experiments using AD models showed that the increased permeability of the BBB was mainly caused by the decreased expression of tight junction-related proteins occludin and claudin-5. In this study, we found that ZNF787 and HDAC1 were upregulated in β-amyloid (Aβ)(1-42)-incubated endothelial cells, resulting in increased BBB permeability. Conversely, the silencing of ZNF787 and HDAC1 by RNAi led to reduced BBB permeability. The silencing of ZNF787 and HDAC1 enhanced the expression of occludin and claudin-5. Mechanistically, ZNF787 binds to promoter regions for occludin and claudin-5 and functions as a transcriptional regulator. Furthermore, we demonstrate that ZNF787 interacts with HDAC1, and this resulted in the downregulation of the expression of genes encoding tight junction-related proteins to increase in BBB permeability. Taken together, our study identifies critical roles for the interaction between ZNF787 and HDAC1 in regulating BBB permeability and the pathogenesis of AD.
摘要：
BACKGROUND: Morgagni hernias are rare anomalies that are easily misdiagnosed or missed. AIM: To summarize the ultrasound (US) imaging characteristics of Morgagni hernias through a comparison of imaging and surgical results. METHODS: The records of children with Morgagni hernias who were hospitalized at two hospitals between January 2013 and November 2023 were retrospectively reviewed in terms of clinical findings, US features, and operative details. RESULTS: Between 2013 and 2023, we observed nine (five male and four female) children with Morgagni hernias. Upper abdominal scanning revealed a widening of the prehepatic space, with an abnormal channel extending from the xiphoid process to the right or left side of the thoracic cavity. The channel had intestinal duct and intestinal gas echoes. Hernia contents were found in the transverse colon (n = 6), the colon and small intestine (n = 2), and the colon and stomach (n = 1). Among the patients, seven had a right-sided lesion, two had a left-sided lesion, and all of them had hernial sacs. CONCLUSION: US imaging can accurately determine the location, extent, and content of Morgagni hernias. For suspected Morgagni hernias, we recommend performing sonographic screening first.
期刊：
UPB Scientific Bulletin, Series C: Electrical Engineering and Computer Science,2024年:1-17 ISSN：2286-3540
通讯作者：
Wen, J
作者机构：
[Wen, Juan; Xiao, Qiankang; Qu, Xing; Lin, Siyu] Univ South China, Sch Elect Engn, Hengyang 421001, Peoples R China.;[Liu, Jie] Univ South China, Sch Comp Software, Hengyang 421001, Peoples R China.
通讯机构：
[Wen, J ] U;Univ South China, Sch Elect Engn, Hengyang 421001, Peoples R China.
关键词：
Active distribution network;Fault location;Dynamic quantum genetic algorithm;Evaluation objective function
摘要：
The fault location of an active distribution network is a vital analysis to prevent major outages in the power system. Considering the influence of renewable distributed generations on fault characteristics, this paper proposes a novel location method based on a dynamic quantum genetic algorithm to solve for fault locations in active distribution networks. In the method, the fault current code is measured based on feeder terminal units. A universal switching function is presented to convert the feeder switch status into an uploaded fault current code. The fault location model is defined as an optimization problem that presents the evaluation objective function with an anti-false-positive factor. The dynamic quantum genetic algorithm is developed to locate the fault feeder according to the uploaded fault current code of the feeder terminal unit. The algorithm adopts dynamic rotating gate strategy and adaptive quantum crossover strategy to satisfy the requirements of quickness and accuracy for fault location. Moreover, the method avoids easily falling into a local optimum by integrating the discrete quantum mutation. The proposed fault location technique is tested and compared to other existing techniques on a 33-bus active distribution network. The simulation results show that the proposed fault location method can locate fault feeders accurately with fast computational times under conditions of single or multiple faults and with an information distortion of the feeder terminal unit.
作者机构：
[Li, Dan; Zhong, Yangyan; Cao, H; Deng, Junjie; Li, Wei; Deng, Jian; Cao, Hong] Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Dept Breast & Thyroid Surg, Hengyang 421001, Hunan, Peoples R China.;[Li, Dan; Zhong, Yangyan; Deng, Junjie; Li, Wei; Deng, Jian; Cao, Hong] Univ South China, Clin Res Ctr Breast & Thyroid Dis Prevent & Contro, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Cao, H ] U;Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Dept Breast & Thyroid Surg, Hengyang 421001, Hunan, Peoples R China.
关键词：
ROS;bioinformatics analysis;breast cancer
摘要：
BACKGROUND: This study aimed to type breast cancer in relation to reactive oxygen species (ROS), clinical indicators, single nucleotide variant (SNV) mutations, functional differences, immune infiltration, and predictive responses to immunotherapy or chemotherapy, and constructing a prognostic model. METHODS: We used uniCox analysis, ConsensusClusterPlus, and the proportion of ambiguous clustering (PAC) to analyze The Cancer Genome Atlas (TCGA) data to determine optimal groupings and obtain differentially expressed ROS-related genes. Clinical indicators were then combined with the classification results and the Chi-square test was used to assess differences. We further examined SNV mutations, and functional differences using gene set enrichment analysis (GSEA) analysis, the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, immune cell infiltration, and response to immunotherapy and chemotherapy. A prognostic model for breast cancer was constructed using these differentially expressed genes, immunotherapy or chemotherapy responses, and survival curves. RT-qPCR was used to detect the differences in the expression of LCE3D, CA1, PIRT and SMR3A in breast cancer cell lines and normal breast epithelial cell line. RESULTS: We identified two distinct tumor types with significant differences in ROS-related gene expression, clinical indicators, SNV mutations, functional pathways, and immune infiltration. The response to specific chemotherapy drugs and immunotherapy treatments also documented significant differences. The prognostic model constructed with 16 genes linked to survival could efficiently divide patients into high- and low-risk groups. The high-risk group showed a poorer prognosis, higher tumor purity, distinct immune microenvironment, and lower immunotherapy response. RT-qPCR results showed that LCE3D, CA1, PIRT and SMR3A are highly expressed in breast cancer. CONCLUSION: Our methodical examination presented an enhanced insight into the molecular and immunological heterogeneity of breast cancer. It can contribute to the understanding of prognosis and offer valuable insights for personalized treatment strategies. Further, the prognostic model can potentially serve as a powerful tool for risk stratification and therapeutic decision-making in clinical settings.
期刊：
FRONTIERS IN IMMUNOLOGY,2024年15:1289644 ISSN：1664-3224
通讯作者：
Li, ZY
作者机构：
[Li, Zhongyu; Fang, Chunxia; Li, ZY; Wang, Xinglv; Wu, Hongrong] Univ South China, Inst Pathogen Biol, Hengyang Med Coll, Sch Nursing,Hunan Prov Key Lab Special Pathogens P, Hengyang, Peoples R China.
通讯机构：
[Li, ZY ] U;Univ South China, Inst Pathogen Biol, Hengyang Med Coll, Sch Nursing,Hunan Prov Key Lab Special Pathogens P, Hengyang, Peoples R China.
关键词：
Chlamydia trachomatis;immune evasion;innate immune cells;innate immunity;survival and growth
摘要：
Chlamydia trachomatis, is a kind of obligate intracellular pathogen. The removal of C. trachomatis relies primarily on specific cellular immunity. It is currently considered that CD4(+) Th1 cytokine responses are the major protective immunity against C. trachomatis infection and reinfection rather than CD8(+) T cells. The non-specific immunity (innate immunity) also plays an important role in the infection process. To survive inside the cells, the first process that C. trachomatis faces is the innate immune response. As the "sentry" of the body, mast cells attempt to engulf and remove C. trachomatis. Dendritic cells present antigen of C. trachomatis to the "commanders" (T cells) through MHC-I and MHC-II. IFN-γ produced by activated T cells and natural killer cells (NK) further activates macrophages. They form the body's "combat troops" and produce immunity against C. trachomatis in the tissues and blood. In addition, the role of eosinophils, basophils, innate lymphoid cells (ILCs), natural killer T (NKT) cells, γδT cells and B-1 cells should not be underestimated in the infection of C. trachomatis. The protective role of innate immunity is insufficient, and sexually transmitted diseases (STDs) caused by C. trachomatis infections tend to be insidious and recalcitrant. As a consequence, C. trachomatis has developed a unique evasion mechanism that triggers inflammatory immunopathology and acts as a bridge to protective to pathological adaptive immunity. This review focuses on the recent advances in how C. trachomatis evades various innate immune cells, which contributes to vaccine development and our understanding of the pathophysiologic consequences of C. trachomatis infection.
摘要：
The electrocatalytic nitrogen reduction reaction (eNRR) under ambient conditions is deemed a promising alternative for NH(3) synthesis. In this paper, an FeP-Fe(3)O(4) nanocomposite electrocatalyst was prepared by phosphating annealing using Fe(2)O(3) as a precursor, and the resulting FeP-Fe(3)O(4) exhibited excellent N(2)-to-NH(3)-producing activity over a wide potential window. The highest faradaic efficiency of FeP-Fe(3)O(4) is 11.02% at -0.1 V vs. reversible hydrogen electrode (RHE), and the maximum NH(3) yield reaches 12.73 μg h(-1) mg(cat)(-1), comparable to or exceeding the reported values in this field. Furthermore, the FeP-Fe(3)O(4) nanocomposite electrocatalyst presents high electrochemical stability, selectivity, and durability.
摘要：
Geopolymer is an amorphous cementitious material produced by geopolymerization using minerals or industrial waste as the main raw material under the action of activators. It can be used for safe treatment of heavy metal and radioactive pollution. This study applied molecular dynamics simulation to investigate the transport behaviour of uranyl nitrate in nanochannel of geopolymer, and focused on the influence of changes in the Si/Al ratio of geopolymer on the adsorption and diffusion of ions and water molecules. The simulation results described by density distribution, radial distribution function (RDF), hydrogen bonding network structure, mean square displacement (MSD) and diffusion coefficient. The density distribution shows that geopolymer has the strongest adsorption capacity for UO22+, followed by NO3-, and finally H2O. The results also indicate that the changes in molecular composition of geopolymer have a significant impact on kinetic characteristics of UO22+, NO3- and H2O. As the Si/Al ratio decreases, the adsorption capacity of geopolymer shows an upward trend, with the most significant improvement in the adsorption of UO22+. In addition, when the Si/Al ratio reduces, the migration rate of UO22+ and NO3- slows down. It could hinder the diffusion of ions in nanopores of geopolymer. The work could optimize the preparation of geopolymer adsorbent, and reveal the transport mechanism of uranyl nitrate in nanopore of geopolymer with different Si/Al ratios at the atomic scale.
摘要：
Chronic obstructive pulmonary disease (COPD) is a heterogeneous lung disease and a major health burden worldwide. Extracellular vesicles (EVs) are nanosized vesicles which possess a lipid bilayer structure that are secreted by various cells. They contain a variety of bioactive substances, which can regulate various physiological and pathological processes and are closely related to the development of diseases. Recently, EVs have emerged as a novel tool for intercellular crosstalk, which plays an essential role in COPD development. This paper reviews the role of EVs in the development of COPD and their potential clinical value, in order to provide a reference for further research on COPD.
摘要：
The emergence of multi-drug-resistant bacteria is threatening to human health and life around the world. In particular, methicillin-resistant Staphylococcus aureus (MRSA) causes fatal injuries to human beings and serious economic losses to animal husbandry due to its easy transmission and difficult treatment. Currently, the development of novel, highly effective, and low-toxicity antimicrobials is important to combat MRSA infections. Thiazole-containing compounds with good biological activity are widely used in clinical practice, and appropriate structural modifications make it possible to develop new antimicrobials. Here, we review thiazole-containing compounds and their antibacterial effects against MRSA reported in the past two decades and discuss their structure-activity relationships as well as the corresponding antimicrobial mechanisms. Some thiazole-containing compounds exhibit potent antibacterial efficacy in vitro and in vivo after appropriate structural modifications and could be used as antibacterial candidates. This Review provides insights into the development of thiazole-containing compounds as antimicrobials to combat MRSA infections.
摘要：
RATIONALE: Retroperitoneal hematomas are relatively common in patients undergoing nephrectomy. Herein, we report an unusual case involving a giant retroperitoneal hematoma and subsequent duodenal ulcerative bleeding following a radical nephrectomy. PATIENT CONCERNS: A 77-year-old woman was admitted to our hospital for lower back pain, and she had severe right hydronephrosis and a urinary tract infection. DIAGNOSES: The patient was diagnosed and confirmed as high-grade urothelial carcinoma. INTERVENTIONS: After ineffective conservative treatments, a right radical nephrectomy and ureteral stump resection were performed. The patient received proton pump inhibitors to prevent stress ulcer formation and bleeding. On the first day post-surgery, she had normal gastrointestinal (GI) endoscopy findings. On the second day post-surgery, abdominal computed tomography revealed a retroperitoneal hematoma. Notably, 14 days post-surgery, massive GI bleeding occurred, and GI endoscopy identified an almost perforated ulcer in the bulbar and descending duodenum. OUTCOMES: The patient died on day 15 after surgery. LESSONS: Duodenal ulceration and bleeding might occur following a retroperitoneal hematoma in patients treated with nephrectomy. Timely intervention may prevent duodenal ulcers and complications, and thus could be a promising life-saving intercession.
作者机构：
[Tang, Xian; Han, Hai] Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.;[Li, Le] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Wang, Hongqing] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Wang, Hongqing] Univ South China, Hunan key Lab Design & Applicat Actinide Complexes, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Tang, X ; Li, L ] U;Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
摘要：
Uranium, highly chemically toxic and radioactive with a long half-life, poses environmental risks due to activities like uranium mining, refining, and nuclear power plant operations. Detecting uranyl ion in real-time is crucial to assess human health risks and control uranium pollution. Electrochemical sensing offers advantages such as sensitivity, selectivity, simplicity, rapidity, and cost-effectiveness, making it ideal for on-site detection of uranyl ion. This paper summarizes recent research progress in electrochemical detection of uranyl. Various techniques including cyclic voltammetry, differential pulse voltammetry, stripping voltammetry, and electrochemical impedance spectroscopy are discussed along with their respective advantages. Detection methods utilizing electrode-modified functional materials like biomaterials, polymers, and nanomaterials are investigated. Probes like DNAzyme, ion-imprinted polymers, and 2D materials are highlighted for their detection mechanism and effectiveness. Electrochemical sensing with these probes achieves nanomolar-level detection limits (nM) with excellent stability and selectivity, suggesting practical applications. Lastly, predictions are made regarding sensing mechanisms, electrode material selection, and adaptability to field conditions for electrochemical detection of uranyl ion.
摘要：
BACKGROUND: In Asian patients with severe ptosis,the use of conjoint fascia sheath (CFS) suspension or levator aponeurosis fascia complex shortening surgery can correct the ptosis. During these surgery, a significant amount of levator aponeurosis fascia shortening is performed, which often leads to serious complications such as conjunctival prolapse.This study compares two surgical approaches for correcting severe blepharoptosis:Conjoint fascial sheath (CFS) + levator aponeurosis and muller's muscle complex (LM complex) suspension and conjoint fascial sheath (CFS) + LM complex+conjunctival suspension.The postoperative efficacy and the incidence of complications such as conjunctival prolapse are investigated for both procedures. METHODS: This study retrospectively analyzed 70 patients (77eyes) with severe blepharoptosis from January 2019 to December 2021. The patients were divided into the experimental group (34 cases, 38 eyes) and the control group (36 cases, 39 eyes). The experimental group was treated with CFS+LM complex + conjunctival suspension, and the control group was treated with CFS+LM complex suspension.The curative effect of blepharoptosis, the incidence of complications such as conjunctival prolapse and patient satisfaction were compared between the two different surgical methods. RESULTS: There was no significant difference in the correction effective rate between the experimental group (84.21%) and the control group (82.05%) (P > 0.05). There was no significant difference in the total incidence of complications between the experimental group (23.68%) and the control group (38.46%) (P > 0.05), but in the complication of conjunctival prolapse, the incidence of conjunctival prolapse in the experimental group was significantly lower than that in the control group. The difference was statistically significant (P < 0.05). In the survey of patient satisfaction rate, the satisfaction rate of the experimental group was significantly higher than that of the control group,which was statistically significant (P < 0.05). CONCLUSIONS: Compared to CFS+LM complex suspension surgery, the CFS+LM complex + conjunctival suspension has a definite effect in preventing postoperative conjunctival prolapse .The procedure has a high feasibility, good corrective effect, and improves patient satisfaction after surgery.
摘要：
Disulfidptosis is a recently identified type of programmed cell death. It is characterized by aberrant accumulation of intracellular disulfides. The clinical implications of disulfidptosis in clear cell renal cell carcinoma (ccRCC) remain unclear. A series of bioinformatics approaches were employed to analyze ten disulfidptosis-related molecules. Firstly, the expression patterns of the disulfidptosis-related molecules were different between normal and ccRCC tissues. A comprehensive cohort of patients with ccRCC was then assembled from three public databases and subjected to cluster analysis based on disulfidptosis-related molecules. Consensus cluster analysis revealed three distinct disulfidptosis clusters. We then conducted weighted gene co-expression network analysis (WGCNA) to identify highly correlated genes. 267 hub genes were screened out through WGCNA, and three gene clusters were then determined. Finally, we identified 87 genes with prognostic value and then used them to develop a disulfidptosis scoring (DSscore) system, which was proven to independently predict survival in ccRCC. Patients in the high-DSscore group exhibited a significant survival advantage and better immunotherapeutic responses compared with those in the low-DSscore group. However, the patients in the low-DSscore group exhibited a greater degree of chemotherapeutic response. In addition, the expression of disulfidptosis-related molecules was validated by qRT-PCR, and the potential of disulfidptosis-related molecules to indicate distinct cell subtypes were validated by single-cell RNA-sequencing. In conclusion, DSscore is a promising index for predicting the prognosis and efficacy of immunotherapy in patients with ccRCC and may provide a basis for novel strategies for future studies.
