作者机构：
[Zeng, Huilan] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Ophthalmol, Hengyang 421001, Hunan, Peoples R China.;[Wadkins, David; Mayberry, Jordan E.; Kuehn, Markus H.; Chen, Nathan] Univ Iowa, Dept Ophthalmol & Visual Sci, Iowa City, IA 52242 USA.;[Wadkins, David; Mayberry, Jordan E.; Kuehn, Markus H.; Chen, Nathan] Iowa City VA Ctr Prevent & Treatment Visual Loss, Iowa City, IA 52246 USA.;[Summers, Daniel W.] Univ Iowa, Dept Biol, Iowa City, IA 52242 USA.
通讯机构：
[Kuehn, MH ] U;Univ Iowa, Dept Ophthalmol & Visual Sci, Iowa City, IA 52242 USA.;Iowa City VA Ctr Prevent & Treatment Visual Loss, Iowa City, IA 52246 USA.
摘要：
Glaucoma is one of the leading causes of irreversible blindness worldwide and vision loss in the disease results from the deterioration of retinal ganglion cells (RGC) and their axons. Metabolic dysfunction of RGC plays a significant role in the onset and progression of the disease in both human patients and rodent models, highlighting the need to better define the mechanisms regulating cellular energy metabolism in glaucoma. This study sought to determine if Sarm1, a gene involved in axonal degeneration and NAD+ metabolism, contributes to glaucomatous RGC loss in a mouse model with chronic elevated intraocular pressure (IOP). Our data demonstrate that after 16 weeks of elevated IOP, Sarm1 knockout (KO) mice retain significantly more RGC than control animals. Sarm1 KO mice also performed significantly better when compared to control mice during optomotor testing, indicating that visual function is preserved in this group. Our findings also indicate that Sarm1 KO mice display mild ocular developmental abnormalities, including reduced optic nerve axon diameter and lower visual acuity than controls. Finally, we present data to indicate that SARM1 expression in the optic nerve is most prominently associated with oligodendrocytes. Taken together, these data suggest that attenuating Sarm1 activity through gene therapy, pharmacologic inhibition, or NAD+ supplementation, may be a novel therapeutic approach for patients with glaucoma.
期刊：
International Journal of Hydrogen Energy,2024年61:415-423 ISSN：0360-3199
通讯作者：
Wang, Shaorong;Jiang, RL;Dai, ZR
作者机构：
[Gao, Yuan; Zhou, Qi; Jiang, Rongli; Wang, Shaorong; Liu, Bo; Zhang, Qiong; Xu, Hanlu; Jiang, RL] China Univ Min & Technol, Sch Chem Engn & Technol, Xuzhou 221116, Jiangsu, Peoples R China.;[Dai, Zhongran] Univ South China, Sch Resource Environm & Safety Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, SR; Jiang, RL ] C;[Dai, ZR ] U;China Univ Min & Technol, Sch Chem Engn & Technol, Xuzhou 221116, Jiangsu, Peoples R China.;Univ South China, Sch Resource Environm & Safety Engn, Hengyang 421001, Peoples R China.
摘要：
The proton exchange membrane (PEM) is vital in proton exchange membrane fuel cells (PEMFCs), but current PEMs face issues including low conductivity, poor durability, and instability in maintaining proton transport across varying temperatures. This study proposes an innovative approach to address this issue by incorporating UiO-66-(COOH)2 as a filler in a polymeric matrix comprising polyvinylpyrrolidone and polyvinylidene fluoride (abbreviated as PP). Significantly, this facilitates forming hydrogen bonds between UiO-66-(COOH)2 and the PP matrix. The UiO-66-(COOH)2@PP composite membranes are comprehensively characterized through PXRD, TGA-DSC, FTIR, and SEM analyses. The remarkable water adsorption performance observed in the UiO-66-(COOH)2@PP-X series creates favourable conditions for enhancing proton conductivity. Proton conductivity studies highlight exceptional performance, particularly UiO-66-(COOH)2@PP-30 achieving a remarkable 5.8 × 10−3 S cm−1 at 98% RH, with sustained conductivity over one week. At 32% RH, UiO-66-(COOH)2@PP-30 exhibits a significantly reduced activation energy of 0.09 eV, indicating a stable proton transport pathway across a wide temperature range. Mechanism studies unveil that the collaborative interaction between UiO-66-(COOH)2 and PP establishes a continuous network of hydrogen bonds, strengthened by PP's hydrophilic properties. Additionally, the stabilization of water within UiO-66-(COOH)2 channels significantly contributes to the overall efficiency of proton conduction.
作者机构：
[Fu, Lichun; Zhen, Deshuai; Yang, Jing; Xiong, Lihao; Luo, Chunhua] Hengyang Market Supervis Inspect & Testing Ctr, Hengyang 421001, Peoples R China.;[Zhen, Deshuai; Xiong, Lihao; Yang, Fei] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Gao, Linxiao; Zhen, Deshuai; Luo, Xiaohu] Qiannan Normal Univ Nationalities, Engn Res Ctr Loss Efficacy & Anticorros Mat Guizho, Sch Chem & Chem Engn, Duyun 558000, Peoples R China.;[Zhou, Huang] Huaihua Univ, Coll Chem & Mat Engn, Huaihua 418000, Peoples R China.;[Yang, Lixia] Nanchang Hangkong Univ, Key Lab Jiangxi Prov Persistent Pollutants Control, Nanchang 330063, Peoples R China.
通讯机构：
[Gao, LX] Q;[Zhen, DS ] H;Hengyang Market Supervis Inspect & Testing Ctr, Hengyang 421001, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;Qiannan Normal Univ Nationalities, Engn Res Ctr Loss Efficacy & Anticorros Mat Guizho, Sch Chem & Chem Engn, Duyun 558000, Peoples R China.
关键词：
Yb@TiO2;Perfluorooctane Sulfonate;Tetrabromobisphenol A;Contaminants;SELDI-TOF MS
摘要：
Ytterbium modified TiO2 nanoparticles (Yb@TiO2), synthesized by a sol-gel process, were employed as an efficient adsorbent and matrix for the analysis of Perfluorooctane sulfonate (PFOS) and Tetrabromobisphenol A (TBBPA) via surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). The detection limits for PFOS and TBBPA are achievement with 0.01 pg center dot mL-1. The results of the analysis of camellia oil samples show good recovery (80.3%-86.8%) with a low detection limit for TBBPA.
摘要：
BACKGROUND: With the burgeoning advancements in disease modeling, drug development, and precision medicine, organ-on-a-chip has risen to the forefront of biomedical research. Specifically in tumor research, this technology has exhibited exceptional potential in elucidating the dynamics of metastasis within the tumor microenvironment. Recognizing the significance of this field, our study aims to provide a comprehensive bibliometric analysis of global scientific contributions related to organ-on-a-chip. METHODS: Publications pertaining to organ-on-a-chip from 2014 to 2023 were retrieved at the Web of Science Core Collection database. Rigorous analyses of 2305 articles were conducted using tools including VOSviewer, CiteSpace, and R-bibliometrix. RESULTS: Over the 10-year span, global publications exhibited a consistent uptrend, anticipating continued growth. The United States and China were identified as dominant contributors, characterized by strong collaborative networks and substantial research investments. Predominant institutions encompass Harvard University, MIT, and the Chinese Academy of Sciences. Leading figures in the domain, such as Dr. Donald Ingber and Dr. Yu Shrike Zhang, emerge as pivotal collaboration prospects. Lab on a Chip, Micromachines, and Frontiers in Bioengineering and Biotechnology were the principal publishing journals. Pertinent keywords encompassed Microfluidic, Microphysiological System, Tissue Engineering, Organoid, In Vitro, Drug Screening, Hydrogel, Tumor Microenvironment, and Bioprinting. Emerging research avenues were identified as "Tumor Microenvironment and Metastasis," "Application of organ-on-a-chip in drug discovery and testing" and "Advancements in personalized medicine applications". CONCLUSION: The organ-on-a-chip domain has demonstrated a transformative impact on understanding disease mechanisms and drug interactions, particularly within the tumor microenvironment. This bibliometric analysis underscores the ever-increasing importance of this field, guiding researchers and clinicians towards potential collaborative avenues and research directions.
摘要：
Iron metabolism plays a crucial role in cell viability, but its relationship with adult stem cells and cancer stem cells is not fully understood. The ferritin complex, responsible for intracellular iron storage, is important in this process. We report that conditional deletion of ferritin heavy chain 1 (Fth1) in the hematopoietic system reduced the number and repopulation capacity of hematopoietic stem cells (HSCs). These effects were associated with a decrease in cellular iron level, leading to impaired mitochondrial function and the initiation of apoptosis. Iron supplementation, antioxidant, and apoptosis inhibitors reversed the reduced cell viability of Fth1-deleted hematopoietic stem and progenitor cells (HSPCs). Importantly, leukemic stem cells (LSCs) derived from MLL-AF9-induced acute myeloid leukemia (AML) mice exhibited reduced Fth1 expression, rendering them more susceptible to apoptosis induced by the iron chelation compared to normal HSPCs. Modulating FTH1 expression using mono-methyl fumarate increased LSCs resistance to iron chelator-induced apoptosis. Additionally, iron supplementation, antioxidant, and apoptosis inhibitors protected LSCs from iron chelator-induced cell death. Fth1 deletion also extended the survival of AML mice. These findings unveil a novel mechanism by which ferritin-mediated iron homeostasis regulates the survival of both HSCs and LSCs, suggesting potential therapeutic strategies for blood cancer with iron dysregulation.
通讯机构：
[Li, T ] U;Univ South China, Sch Resource Environm & Safety Engn, Hengyang 421001, Peoples R China.
摘要：
Low-concentration electrolyte demonstrates significant advantages in terms of low cost, low viscosity, and high wettability. However, its high solvent ratio usually induces a fragile organic-rich solid electrolyte interphase (SEI), which is not compatible with high-voltage lithium metal batteries (LMBs). Herein, a screening rule for diluents has been established based on the electrostatic potential, which guides the rational design of diluted low-concentration electrolyte (DLCE) with a high anion-to-solvent ratio. Specifically, the anti-solvating power of a diluent is positively correlated with its value of the most positive surface potential (ESPmax), and thus diluents with a high ESPmax value easily cause salt precipitation when diluting concentrated electrolyte to low concentration (<1 M). In light of this observation, a diluent with a low ESPmax value is selected to ensure the preparation of a transparent DLCE, thus maintaining a high anion-to-solvent ratio in DLCE. As an exemplary study, a 0.5 M ether-based DLCE is developed for high-voltage LMBs. Due to the high anion-to-solvent ratio, a robust LiF-rich SEI is formed and enables the stable operation of LMBs under high voltage (4.5 V) and a wide temperature range (-20 to 55 C-degrees). This work offers a guideline for screening diluents to design high-performance LCEs for high-voltage batteries.
摘要：
BACKGROUND: Depression is a highly prevalent comorbidity arising in patients with Parkinson's disease (PD). However, depression in patients with PD is poorly treated. Hydrogen sulfide (H(2)S), a neuromodulator, has the potential to relieve depression. OBJECTIVE: To investigate whether H(2)S attenuates depression-like behaviours in a rat model of PD and examine the underlying mechanisms. METHODS: We utilised rotenone to develop a PD model with subcutaneous injections in the dorsal cervical region of Sprague-Dawley rats. The depression-like behaviours in the rotenone-induced PD model rats were assessed through forced swimming, tail suspension, open field, novelty-suppressed feeding, and elevated plus-maze tests. The expression of postsynaptic density protein-95 and synapsin-1, related to synaptic plasticity, was detected using Western blot in the hippocampus. The hippocampal ultrastructure, including the synaptic density, length of the synaptic active zone, postsynaptic density thickness, and synaptic gap width, was detected using transmission electron microscopy. RESULTS: We proved that sodium hydrosulfide (NaHS; a donor of H(2)S) significantly attenuated the depression-like behaviours and disorders of hippocampal synaptic plasticity in rotenone-induced PD rats. Furthermore, inhibition of the hippocampal Warburg effect by 2-deoxyglucose abolished NaHS-enhanced hippocampal synaptic plasticity and reversed NaHS-attenuated depression-like behaviours in the rotenone-induced PD rats. CONCLUSION: H(2)S attenuates PD-associated depression by improving the hippocampal synaptic plasticity in a hippocampal Warburg effect-dependent manner.
摘要：
In Particle-In-Cell simulation, macro-particles represent clusters of numerous physical particles. Rational merging or splitting of these macro-particle clusters can significantly improve the efficiency of simulation and reduce unnecessary computational requirements and memory consumption. Here, we propose a dynamical particle merging and splitting algorithm. For macro-particle in different density regions, regional macro-particle clusters are obtained by dividing them with the Minkowski metric function. Dynamic merging and splitting of macro-particles is achieved by dynamically changing the number of macro-particle clusters to reduce the number of macro-particles without substantially distorting the physical description of the system. To test the capability of the algorithm, we also compare its performance under three types of classical plasma cases: two-stream instability (1D), QED cascades (2D), and magnetic shower (3D). The results show a good agreement with expectations. (c) 2023 Elsevier B.V. All rights reserved.
关键词：
Germanium;Tartaric acid;Selective separation;Column separation;D201 x 7 resin
摘要：
Germanium (Ge) exhibits excellent optical and electrical properties. Due to the low reserves of Ge and lack of independent mineral resources, zinc refinery residues have become an important source of Ge. In this work, a D201 x 7 resin has been employed in the selective separation of Ge from a sulfuric solution using tartaric acid as a complexing agent. In the sulfuric acid system, Ge(OH)4 was converted to an anionic complex [GeO2(OH)2C4H4O4]2- at a 1:1 tartaric acid to Ge molar ratio. The anion exchange D201 x 7 resin adsorbed the Ge tartrate anionic complex. The adsorption process exhibited pseudo-second-order kinetic behavior and adhered to the Langmuir isothermal adsorption model. The maximum adsorption capacity of D201 x 7 resin for Ge was 214 mg g-1. Selective Ge uptake and purification could proceed by column separation The results showed that enrichment factor was 74.7, and the desorption concentration of Ge was increased from 500 mg L-1 to 36.6 g L-1. The D201 x 7 resin exhibited high selectivity, excellent adsorption performance and good stability, making it a viable candidate in industrial applications.
期刊：
European Journal of Pharmacology,2024年972:176558 ISSN：0014-2999
通讯作者：
Hu, Jinyue
作者机构：
[Wang, Yufei; Xiang, Wei; Jiang, Manli; Liu, Xueting] Medical Research Center, Affiliated Changsha Central Hospital of Hengyang Medical School, University of South China, Changsha, 410004, China;[Wang, Guihua] Department of Oncology, Affiliated Changsha Central Hospital of Hengyang Medical School, University of South China, Changsha, 410004, China;[Hu, Jinyue] Medical Research Center, Affiliated Changsha Central Hospital of Hengyang Medical School, University of South China, Changsha, 410004, China. Electronic address: 2018050703@usc.edu.cn
通讯机构：
[Hu, Jinyue] M;Medical Research Center, Affiliated Changsha Central Hospital of Hengyang Medical School, University of South China, Changsha, 410004, China. Electronic address:
摘要：
Inhibitors of polo-like kinase (PLK) are currently being evaluated as anticancer drugs. However, the molecular mechanism of PLK inhibitor-induced cell death is not fully understood. In this study, we found that GW843682X and BI2536, two inhibitors of PLK1, significantly induced cell death in multiple type cells. The induction of cell death was related to the preferring expression of PLK1. However, in human umbilical vascular endothelial cells (HUVEC) and human colorectal carcinoma cells, which expressed higher levels of both PLK1 and PLK2, PLK1 inhibitors induced very low levels of cell death. Clinical analysis reveals PLK1 presence in 26 of 30 NPC tumor tissues. In in vivo NPC lung metastasis nude mouse models, PLK1 inhibitors decreased NPC progress. Mechanistically, the PLK1 inhibitor did not activate p53, and the cell death was not reversed by p53 inhibition. Moreover, PLK1 inhibitor-induced cell death was PARP- and caspase-independent. Although PLK1 inhibitors induced down-regulation of calpain inhibitor calpastatin and calpain was activated by PLK1 inhibition, calpain blocking did not reverse cell death induced by PLK1 inhibitors, suggesting the non-involvement of calpain. Surprisingly, we found that PLK1 inhibitors induced the activation of proteasome, and the treatment of cells with PLK1 inhibitors reduced the levels of ubiquitinated proteins. And proteasome inhibitors reversed cell death induced by PLK1 inhibitors in various cell types in which PLK1 was preferentially expressed. Moreover, PLK1 inhibition reversed the degradation of proteins including p53, caspase 8, PARP and calpastatin. These results suggest that the activation of proteasome is critical for cell death induced by PLK1 inhibition.
摘要：
Metal halide perovskite (MHP) detectors are highly esteemed for their outstanding photoelectric properties and versatility in applications. However, they are unfortunately prone to degradation, which constitutes a significant barrier to their sustained performance. This review meticulously delves into the causes leading to their instability, predominantly attributable to factors such as humidity, temperature, and electric fields and, notably, to various radiation factors such as X-rays, gamma-rays, electron beams, and proton beams. Furthermore, it outlines recent advancements in strategies aimed at mitigating these detrimental effects, emphasizing breakthroughs in composition engineering, heterostructure construction, and encapsulation methodologies. At last, this review underscores the needs for future improvements in theoretical studies, material design, and standard testing protocols. In the pursuit of optimizing the chemical stability of MHP detectors, collaborative efforts are in an imperative need. In this way, broad industrial applications of MHP detectors could be achieved.
作者机构：
[Peiguan Huang; Xiaojun Tang] Second Affiliated Hospital, Department of Joint Surgery, Hengyang Medical School, University of South China, Hengyang, Hunan, China
通讯机构：
[Peiguan Huang] S;Second Affiliated Hospital, Department of Joint Surgery, Hengyang Medical School, University of South China, Hengyang, Hunan, China
摘要：
At present, the suture bridge is a widely used surgical pattern in the treatment of supraspinatus tendon tear, but the shortcomings of a suture bridge, including expensive lateral-row anchor and increased type 2 retear rate, is obvious. The double-pulley suture-bridge described in this Technical Note uses a double-loaded suture anchor (medial-row anchor) as lateral-row anchor instead of traditional lateral-row anchor, combined with double-pulley technology forming suture-bridge in treatment of supraspinatus tendon tears. The surgical technique is described in pearls, pitfalls, advantages, and disadvantages.
作者机构：
[Liu, Li; He, Jingzhe; Wang, Jiaren; Li, Ruining; Li, Qimei; Zeng, Lin; Xiao, Lushan; Li, Yan; Hong, Chang; Cui, Hao] Southern Med Univ, Nanfang Hosp, Hepatol Unit, Guangdong Prov Key Lab Viral Hepatitis Res, Guangzhou, Peoples R China.;[Liu, Li; He, Jingzhe; Wang, Jiaren; Li, Ruining; Li, Qimei; Zeng, Lin; Xiao, Lushan; Li, Yan; Hong, Chang; Cui, Hao] Southern Med Univ, Nanfang Hosp, Dept Infect Dis, Guangzhou, Peoples R China.;[Liu, Li; Wang, Jiaren; Xiao, Lushan] Southern Med Univ, Nanfang Hosp, Big Data Ctr, Guangzhou, Peoples R China.;[Wang, Weizhen] Southern Med Univ, Nanfang Hosp, Dept Ultrasound, Guangzhou, Peoples R China.;[Zhu, Hongbo] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Med Oncol, Hengyang, Hunan, Peoples R China.
通讯机构：
[Xiao, LS ; Liu, L ; Zhu, H ] S;Southern Med Univ, Nanfang Hosp, Guangzhou 510515, Peoples R China.;Southern Med Univ, Nanfang Hosp, Big Data Ctr, Guangzhou 510515, Peoples R China.;Southern Med Univ, Nanfang Hosp, Hepatol Unit, Guangdong Prov Key Lab Viral Hepatitis Res, Guangzhou 510515, Peoples R China.;Southern Med Univ, Nanfang Hosp, Dept Infect Dis, Guangzhou 510515, Peoples R China.
关键词：
CCR;MSFLD;risk factor;UK Biobank
摘要：
OBJECTIVE: Metabolic dysfunction-associated steatotic liver disease (MASLD) affects many populations, and screening out the high-risk populations at an early stage is a challenge. As a sarcopenia index, the relationship between creatinine to cystatin C ratio (CCR) and MASLD remains unclear. This cross-sectional, prospective study aimed to explore the relationship between CCR and MASLD. Design Firstly, explored the correlation between CCR and MASLD in cross-sectional analyses. Then excluded the population with baseeline diagnosis of MASLD and analyzed the association with baseline CCR levels and the onset of MASLD in the population with available follow-up data. Univariate and multivariate logistic regression analyses were used to calculate odds ratios (ORs) to evaluate the association between CCR levels and MASLD. PATIENTS AND MEASUREMENTS: This study included 368,634 participants from the UK Biobank for cross-sectional and prospective analyses. The demographic characteristics and laboratory measurements of all participants were obtained from the UK Biobank. MASLD was diagnosed according to the multi-society consensus nomenclature. Hepatic steatosis was defined as FLI ≥60. RESULTS: We grouped the study participants according to CCR tertiles. In cross-sectional analyses, participants in CCR tertile 1 had the highest MASLD risk (OR: 1.070, 95%CI: 1.053-1.088, p < .001). And the similar association was observed in the prospective analyses (CCR tertile 1 OR: 1.340, 95%CI: 1.077-1.660, p = .009; CCR tertile 2 OR: 1.217, 95%CI: 1.021-1.450, p = .029, respectively). After stratification by gender, the significant association between CCR and the onset of MASLD was only observed in males (CCR tertile 1 OR: 1.639, 95%CI: 1.160-2.317, p = .005; CCR tertile 2 OR: 1.322, 95%CI: 1.073-1.628, p = .005, respectively). CONCLUSION: Our results indicated that lower CCR was significantly associated with higher risk of MASLD, based on which predictive models can be developed to screen populations at high risk of developing MASLD.
通讯机构：
[Chen, GD; Wu, DC ] U;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Hepatopancreatobiliary Surg,Lab Struct Immuno, Hengyang 421001, Hunan, Peoples R China.
关键词：
Combination therapy;Therapeutic cancer vaccine;Tumor antigen
摘要：
Therapeutic cancer vaccines have shown promising efficacy in helping immunotherapy for cancer patients, but the systematic characterization of the clinical application and the method for improving efficacy is lacking. Here, we mainly summarize the classification of therapeutic cancer vaccines, including protein vaccines, nucleic acid vaccines, cellular vaccines and anti-idiotypic antibody vaccines, and subdivide the above vaccines according to different types and delivery forms. Additionally, we outline the clinical efficacy and safety of vaccines, as well as the combination strategies of therapeutic cancer vaccines with other therapies. This review will provide a detailed overview and rationale for the future clinical application and development of therapeutic cancer vaccines.
作者机构：
[Ya Guo] Southwest Jiaotong University, 111 Second Ring, North 1st Section, Chengdu, Sichuan, 610031, CHINA;[Hiroaki Nakajima; Wenbin Lin] University of South China, 28 Changsheng West Road, Hengyang, Hunan, 421001, CHINA
摘要：
We study the wave equations with various spins on the background of the general spherically symmetric spacetime. We obtain the unified expression of the Teukolsky-like master equations and the corresponding radial equations with the general spins. We also discuss the gauge dependence in the gravitational-wave equations, which have appeared in the previous studies.
