期刊：
Molecular Systems Design & Engineering,2024年 ISSN：2058-9689
通讯作者：
Shuang-Shuang Long<&wdkj&>Ying-Wu Lin
作者机构：
School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China +86 743 8282375;[Lu Yu] High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, Anhui 230031, China;[Shu-Qin Gao] Key Lab of Protein Structure and Function of Universities in Hunan Province, University of South China, Hengyang 421001, China;[Yan-Yan Li; Yu Feng; Shuang-Shuang Long] School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China;[Ying-Wu Lin] School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China<&wdkj&>Key Lab of Protein Structure and Function of Universities in Hunan Province, University of South China, Hengyang 421001, China
通讯机构：
[Shuang-Shuang Long; Ying-Wu Lin] S;School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China<&wdkj&>Key Lab of Protein Structure and Function of Universities in Hunan Province, University of South China, Hengyang 421001, China<&wdkj&>School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China
摘要：
Human cytochrome c (hCyt c) contains a covalently attached heme group with six-coordination (Met/His) and plays vital biological functions, including electron transfer and peroxidase activity by structural alterations, as well as other functions by interactions with partners such as neuroglobin (Ngb). In this study, we designed and engineered an artificial disulfide bond in hCyt c via double mutations (A51C/G77C) which bridges the Ω-loops C and D. The formation of the intramolecular disulfide bond (Cys51–Cys77) was confirmed by mass spectrometry. The molecular modeling study showed that the disulfide bond did not alter the overall structure, and the local structure where Cys51 was located was well folded into an α-helix. Spectroscopic studies were also performed to probe the effects of the disulfide bond on the protein structure, which revealed that the heme coordination of Met80 was likely weakened. Consequently, the rate of ligand binding and the peroxidase activity were enhanced. Meanwhile, the interaction between hCyt c and Ngb was weakened, as suggested by titration studies. These observations indicate that the dynamic properties of Ω-loops C and D may favor the heme coordination and protein–protein interactions by conformational change, which supports the native functions of hCyt c.
摘要：
The effects of reaction temperature, reaction time, NaOH concentration and dosage on the hydrolysis rate of TBP were investigated. With the optimized conditions, the hydrolysis rate of TBP reached more than 99.5%. The reaction temperature is the greatest impact factor of TBP hydrolysis. As the hydrolysis reaction proceeded, TBP was first hydrolyzed into NaDBP, and then further hydrolyzed into Na2MBP. Three phases were formed after alkaline hydrolysis of TBP/n-dodecane, the n-dodecane (approximate to 70% v%) was at the top phase that could be recycled or reused.
摘要：
An innovative MnO/Ti3C2 MXene heterostructure, consisting of three-dimensional (3D) MnO nanosheet array directly assembled on two-dimensional (2D) Ti3C2 MXene nanosheets, was synthesized via a simple hydrothermal-annealing strategy for uranium elimination. This unique 3D/2D heterostructure derived substantially high specific surface area (311.9 m2/g) and exposed considerable amount of surface oxygen groups, including surface lattice M-O and adsorbed -OH. Through a comprehensive exploration encompassing adsorbent dosage, pH, ionic strength, initial U(VI) concentration, and environmental conditions, the MnO/Ti3C2 MXene heterostructure demonstrated an extraordinary proficiency and specificity in the removal of U(VI). This composite exhibited an outstanding adsorption capacity of 862.1 mg/g, with a removal efficiency of 99.7 % and rapid kinetics (above 90 % within 20 s). The kinetic study indicated the adsorption process followed chemisorption dynamics and single -layer adsorption behavior. Comprehensive analyses validated the structural integrity and confirmed the successful uranium adsorption via strong interaction between UO22+ and surface unsaturated oxygen groups. Notably, the MnO/Ti3C2 MXene maintained excellent recyclability, highlighting its potential for large-scale applications. It emerges as a promising candidate for uranium removal, offering insights for optimizing composite materials in diverse environmental conditions and fostering advancements in water purification technologies.
摘要：
LaFe1-xMnxO3 (0 <= x <= 0.9) samples were synthesized through the sol-gel method. According to X-ray diffraction (XRD) results, with the increase of Mn doping, the crystal structure of the sample changes from orthogonal Pnma structure to rhombohedral R3c structure, and a two-phase coexistence phenomenon exists within the range of 0.4 <= x <= 0.7. Using Mossbauer spectroscopy, the coexistence range of the two phases and the iron content in each phase were identified. At x=0.4, the Pnma phase contribution (91.1%) dominates, with only a slight R3c phase contribution (8.9%). As the amount of Mn doping gradually increases, the R3c phase becomes dominant at x=0.6 (74.6%), and the Pnma phase completely disappears at x=0.8. This work will provide an important perspective for future researchers studying similar phase coexistence systems.
摘要：
In order to improve the utilization of spent fuel, molten salt electrolysis is used to separate lanthanides from spent fuel. The electrochemical reduction mechanism of Ce(III) ions and dynamic properties of Ce(III)/Ce(0) were provided in the LiCl-KCl molten salt by diversified electrochemical techniques. The diffusion coefficient of Ce(III) and the exchange current densities of Ce(III)/Ce(0) on the W electrode were calculated by cyclic vol-tammetry and linear polarization, and the reaction activation energy was calculated to be 30.8 kJ center dot mol-1. The electrochemical behaviors were measured in the LiCl-KCl-CeCl3-K2ZrF6 molten salt on the W electrode and LiCl-KCl-CeCl3 molten salt on the Zr electrode at 753 K. The redox mechanism of Ce(III) ions was studied on the Zr electrode. Compared the reaction at different electrodes, the reduction potential of cerium on the Zr electrode is more correct than that on the W electrode, the underpotential displacement of cerium and zirconium due to the formation of alloy is 0.26 V, which was detecteted using cyclic voltammetry, square wave voltammetry and chronopotentiometry. In addition, the feasibility of extracting Ce on the Zr electrode by potentiostatic elec-trolysis at-1.9 V and-2.2 V in the LiCl-KCl molten salt. The cathodic deposition output was characterized by XRD and SEM-EDS, Ce-Zr solid solution was derived under the different conditions. Simultaneously, the Ce metal was discovered when the deposition potential at-2.2 V. The ICP-OES results showed that the extraction ratio of was about 94.02 % for Ce(III) after potentiostatic electrolysis at-2.2 V for 5 h.
摘要：
Reconstruction of pressure-temperature-time (P-T-t) path of volumetrically-dominant orthogneisses in deeply subducted continental crusts is challenging due to their simple amphibolite-facies mineral assemblages. To provide new insights into this issue, a detailed study of mineral inclusions, trace elements and U-Pb ages within robust zircon containers, together with titanite U-Pb dating and petrology were conducted on ultrahigh-pressure (UHP) orthogneisses from the northern Sulu belt. The following P-T-t conditions of three metamorphic stages were constrained: (i) cathodoluminescence (CL)-grey zircon cores (ca. 238-237 Ma) with flat heavy rare earth element (HREE) patterns include garnet, phengite and omphacite that yield estimated P-T conditions of 2.3-2.8 GPa/730-800 degrees C, corresponding to prograde high-pressure (HP) metamorphism before the UHP peak. (ii) CLdark zircon mantles (ca. 224 Ma) contain multiphase solid inclusions of plagioclase +K-feldspar + quartz and show extremely low Th/U ratios (<= 0.08) and negative Eu anomalies, demonstrating their crystallization from hydrous melt at 2.0-1.8 GPa/890-820 degrees C during HP eclogite-facies exhumation. In addition, titanite grains are characterized by high LREE contents (1939-5239 ppm), Th/U ratios (0.37-0.81) and crystallization temperatures (830-801 degrees C), suggesting their peritectic origin. The lower intercept age (220 +/- 4 Ma) of titanite is broadly consistent with the ages of the zircon mantles, indicating that they both crystallized from the melt at the same stage. (iii) CL-bright zircon rims (ca. 212-211 Ma) preserve low-pressure mineral inclusions of plagioclase, Kfeldspar and quartz, and they show low Th/U ratios (0.03-0.12) and steep HREE patterns with negative Eu anomalies, witnessing amphibolite-facies overprinting at 1.0-0.8 GPa/610-550 degrees C. Combined with the metamorphic peak conditions of 3.2 GPa/840 degrees C at ca. 232 Ma constrained by previous studies from the same area, the P-T-t path of the northern Sulu UHP belt is reconstructed. The data reflects two-stage exhumation of this terrane, including an early-stage (5.8 km/m.y.) and a later-stage slower (3.1 km/m.y.) exhumation processes. The faster exhumation during early decompression was probably enhanced by extensive melting activity recorded by the CL-dark zircon mantles from the orthogneisses.
作者机构：
[Jin, Liang; Zhai, Jinxia; Wang, Chao; Liu, Mingjie] Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, China;[Chen, Yongjun] Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, China. Electronic address: 2212680954@qq.com
通讯机构：
[Chen, Yongjun] D;Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, China. Electronic address:
摘要：
OBJECTIVE: This review aims to provide a comprehensive summary of the latest research progress regarding the relationship between epilepsy and circular RNA (circRNA). METHODS: Relevant literature from the PubMed database was meticulously searched and reviewed. The selected articles focused on investigating the association between epilepsy and circRNA, including studies on expression patterns, diagnostic markers, therapeutic targets, and functional mechanisms. RESULTS: Epilepsy, characterized by recurrent seizures, is a neurological disorder. Numerous studies have demonstrated significant alterations in the expression profiles of circRNA in epileptic brain tissues, animal models, and peripheral blood samples. These differential expressions of circRNA are believed to be closely linked with the occurrence and development of epilepsy. Moreover, circRNA has shown promising potential as diagnostic markers for epilepsy, as well as prognostic indicators for predicting disease outcomes. Furthermore, circRNA has emerged as a potential therapeutic target for epilepsy treatment, offering prospects for gene therapy interventions. CONCLUSION: The dysregulation of circRNA expression in epilepsy suggests its potential involvement in the pathogenesis and progression of this disorder. Identifying specific circRNA molecules associated with epilepsy may pave the way for novel diagnostic approaches and therapeutic strategies. However, further investigations are imperative to elucidate the precise functional mechanisms of circRNA in epilepsy and validate its clinical utility.
摘要：
Measurements of radon exhalation rate using traditional methods can be affected by back-diffusion or differential pressure in the accumulation chamber, resulting in deviations between the measured and the true values. To obtain an accurate radon exhalation rate for evaluation of radon-risk regions, two novel approaches of measurements based on traditional methods were proposed. Repeated experiments were implemented on a self-designed stainless cylindrical vessel filled with uranium tailings sand. The measured radon exhalation rates on average were 0.51±0.02 and 0.52±0.02Bqm(-2) s(-1) for the two proposed methods, with 0.02% and 0.04%, respectively, deviations from the theoretical value. In addition, numerical techniques were employed to interpret the defects of traditional methods and mechanisms of proposed approaches to measure accurate values. Two novel approaches have significantly reduced the impact of back diffusion and differential pressure inside the chamber and consumed less time.
作者机构：
[Zhang, Pengcheng; Zhou, Pengfei] Univ South China, Sch Elect Engn, Hengyang 421200, Peoples R China.
通讯机构：
[Zhang, PC ] U;Univ South China, Sch Elect Engn, Hengyang 421200, Peoples R China.
摘要：
The Li(2)Mg(3)Ti(1-x)Al4(x/3)O(6) (0 <= x <= 0.1) ceramics are synthesized using the solid phase method, showing excellent microwave dielectric properties with epsilon(r) = 14.05, Q x f = 144 500 GHz, and tau(f )= -43 ppm/C-degrees at x = 0.04. Rietveld refinements, based on the XRD data, reveal that the Li(2)Mg(3)Ti(1-x)Al4(x/3)O(6) ceramics crystallize in the rock salt structure with Fm-3m space group. The relationship between the crystal structure and intrinsic microwave dielectric properties is studied in detail. The variation in the dielectric constant is related to ionic polarization and relative density. The quality factor (Q x f) demonstrates a strong correlation with lattice energy, relative density, and concentration of Ti3+. The variation of tau f is influenced by the bond thermal expansion coefficient and bond valence. The dielectric resonant antenna, manufactured from Li2Mg3Ti0.96Al0.0533O6-0.09TiO(2) ceramic with near-zero tau f, shows a bandwidth of 230 MHz at 7.40 GHz and a high radiant efficiency, which indicates that high-quality factor Li2Mg3Ti0.96Al0.0533O6 ceramic has significant potential in relevant fields such as 5G and smart driving.
摘要：
Quinic acid (QA) and its derivatives have good lipid-lowering and hepatoprotective functions, but their role in atherosclerosis remains unknown. This study attempted to investigate the mechanism of QA on atherogenesis in Apoe−/− mice induced by HFD. HE staining and oil red O staining were used to observe the pathology. The PCSK9, Mac-3 and SM22a expressions were detected by IHC. Cholesterol, HMGB1, TIMP-1 and CXCL13 levels were measured by biochemical and ELISA. Lipid metabolism and the HMGB1-SREBP2-SR-BI pathway were detected by PCR and WB. 16 S and metabolomics were used to detect gut microbiota and serum metabolites. QA or low-frequency ABX inhibited weight gain and aortic tissue atherogenesis in HFD-induced Apoe−/− mice. QA inhibited the increase of cholesterol, TMA, TMAO, CXCL13, TIMP-1 and HMGB1 levels in peripheral blood of Apoe−/− mice induced by HFD. Meanwhile, QA or low-frequency ABX treatment inhibited the expression of CAV-1, ABCA1, Mac-3 and SM22α, and promoted the expression of SREBP-1 and LXR in the vascular tissues of HFD-induced Apoe−/− mice. QA reduced Streptococcus_danieliae abundance, and promoted Lactobacillus_intestinalis and Ileibacterium_valens abundance in HFD-induced Apoe−/− mice. QA altered serum galactose metabolism, promoted SREBP-2 and LDLR, inhibited IDOL, FMO3 and PCSK9 expression in liver of HFD-induced Apoe−/− mice. The combined treatment of QA and low-frequency ABX regulated microbe-related Glycoursodeoxycholic acid and GLYCOCHENODEOXYCHOLATE metabolism in HFD-induced Apoe−/− mice. QA inhibited TMAO or LDL-induced HCAECs damage and HMGB1/SREBP2 axis dysfunction, which was reversed by HMGB1 overexpression. QA regulated the gut-liver lipid metabolism and chronic vascular inflammation of TMA/TMAO through gut microbiota to inhibit the atherogenesis in Apoe−/− mice, and the mechanism may be related to the HMGB1/SREBP2 pathway.
期刊：
Journal of Materials Chemistry A,2024年12(6):3331-3339 ISSN：2050-7488
通讯作者：
Ju, J;Qian, XT
作者机构：
[Huang, Chenhui; Kang, Xiaomin; Zhang, Meng] Univ South China, Coll Mech Engn, Hengyang 421001, Peoples R China.;[Zhai, Zhaofeng] Chinese Acad Sci, Inst Met Res, Shenyang 110012, Peoples R China.;[Ju, Jiang] City Univ Hong Kong, Ctr Adv Nucl Safety & Sustainable Dev, Hong Kong 999077, Peoples R China.;[Qian, Xitang; Qian, XT] Hong Kong Univ Sci & Technol, Dept Chem & Biol Engn, Kowloon, Hong Kong, Peoples R China.
通讯机构：
[Qian, XT ] H;[Ju, J ] C;City Univ Hong Kong, Ctr Adv Nucl Safety & Sustainable Dev, Hong Kong 999077, Peoples R China.;Hong Kong Univ Sci & Technol, Dept Chem & Biol Engn, Kowloon, Hong Kong, Peoples R China.
摘要：
Nanofluidic ion regulation membranes have emerged as versatile platforms for applications in molecular/ion separation and energy conversion. The use of two-dimensional (2D) material-based membranes holds great potential for the regulation of nanofluidic ions owing to their unique properties of surface charges, nanochannels, and nanocapillary force. Herein, a class of 2D flexible ion-conductive membranes with surface charge-controllable and voltage-tunable ion transport properties, which are assembled with monolayered Cd vacancy-containing CdPS3 (vc-CdPS3)-based nanosheets, is reported. Importantly, the ion conductivity of the vc-CdPS3 membrane is several orders of magnitude higher than that of bulk salt solutions up to 0.1 M and reaches a plateau of similar to 10 mS cm(-1) in low concentrated solution (<= 1 mM), demonstrating typical charge-controllable nanofluidic ion transport behavior. This membrane exhibits excellent stability and maintains an ion conductivity of 23 and 20 mS cm(-1) under harsh acidic and alkaline conditions, respectively. By applying positive/negative gating voltage, ion transportation within the vc-CdPS3 membrane is tuned, resulting in low/high ion conductivity. The voltage-tunable behavior across a broad spectrum of cations with varying sizes and charges is observed, showcasing the ion-specific switch ratios of 12 and 10 for potassium and sodium ions, respectively, under an applied voltage of 2 V/-2 V. This work demonstrates the potential of vacancy-containing membranes for a variety of membrane separation applications and offer a strategy for preparing efficient ion transport devices.
通讯机构：
[Peng, AG ] U;Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.
关键词：
(137)Cs;(239+240)Pu;Distribution characteristics;Lake East dongting;Lake sediments
摘要：
Plutonium-239+240 and (137)Cs in the environment can usually be used to track the impact of nuclear activities on the environment, and have become important tools in environmental geochemical studies. In this study, nine sediment cores (E1-E9) in Lake East Dongting were collected and measured for the activity concentration of (239+240)Pu, (137)Cs and the atomic ratio of (240)Pu/(239)Pu, and then their vertical distribution characteristics were analyzed. The results show that: the activity concentrations of (137)Cs and (239+240)Pu in Lake East Dongting ranged from 5.26±0.43 to 28.6±2.23 Bq kg(-1) and 0.29±0.02 to 1.37±0.09 Bq kg(-1), with an average of 7.48±0.68 Bq kg(-1) and 0.39±0.03 Bq kg(-1), respectively. The atomic ratios of (240)Pu/(239)Pu are 0.168±0.012-0.211±0.015, which are basically consistent with the global atmospheric deposition. The vertical profiles of (137)Cs and (239+240)Pu in sediment cores show obvious single-peak distribution in E1-E6 and bimodal distribution in E7-E9. The results of sedimentation rates calculated by (137)Cs and (239+240)Pu method ranged from 0.59cm y(-1) to 1.99cm y(-1) with a mean of 1.18cm y(-1) and 0.61cm y(-1) to 2.18cm y(-1) with a mean of 1.26cm y(-1). The inventories of (137)Cs and (239+240)Pu in nine sediment cores are 5.87-10.8 kBq m(-2) and 307-545 Bq m(-2), which are about 8-14 and 9-15 times the inventory in the global average atmospheric deposition at the same latitude respectively. Comparing the results of the sedimentation rates and the inventories from different sampling points indicates that extreme climatic events and human activities have a significant impact on sediment environment of Lake East Dongting.
期刊：
Environmental Science and Pollution Research,2024年:1-14 ISSN：0944-1344
通讯作者：
Lu, Yue
作者机构：
[Liangqin Wang; Haichao Sha; Taotao Zeng; Haidu Nong] Hunan Province Key Laboratory of Pollution Control and Resources Reuse Technology, University of South China, Hengyang, 421001, Hunan, China;[Lin Tang; Taotao Zeng; Qingqing Xie] College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China;[Lin Tang; Taotao Zeng; Qingqing Xie] Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, Hunan, China;[Yue Lu] College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China. yuelu@hnu.edu.cn;[Yue Lu] Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, Hunan, China. yuelu@hnu.edu.cn
通讯机构：
[Lu, Yue] C;College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China.;Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, Hunan, China.
关键词：
Heavy metal;Lead–zinc mining;Microbial community;Microbial interaction;Mine soil
摘要：
Understanding the microbial community structure in soil contaminated with heavy metals (HMs) is a precondition to conduct bioremediation in mine soil. Samples were collected from a typical lead-zinc (Pb-Zn) mine to assess the microbial community structure of the HMs concentrated in the soil. The goal was to analyze the bacterial and fungal community structures and their interactions using the 16S rRNA genes and internal transcribed spacer high-throughput sequencing. Analyses at different sampling sites showed that contamination with HMs significantly reduced the bacterial richness and diversity but increased that of the fungi. The predominant bacteria genera of Acidobacteriales, Gaiellales, Anaerolineaceae, Sulfurifustis, and Gemmatimonadaceae, and predominant fungal genera of Sordariomycetes, Talaromyces, and Mortierella were assumed as HM resistant genera in Pb-Zn mining area. The pH effect on the bacterial and fungal communities was opposite to those of Cd, Pb, and Zn. This study offers comprehensive outlooks for bacterial and fungal community structures upon multiple HM stresses in the soil around a typical Pb-Zn mine area.
摘要：
Coronavirus Disease 2019 (COVID-19) is generally susceptible to the population, highly infectious, rapidly transmitted, and highly fatal. There is a lack of specific drugs against the virus at present and vaccination is the most effective strategy to prevent infection. However, studies have found that some groups, particularly patients with diabetes, show varying degrees of weak immune reactivity to various COVID-19 vaccines, resulting in poor preventive efficacy against the novel coronavirus in patients with diabetes. Therefore, in this study, patients with type 2 diabetes mellitus (T2DM) who had weak immune response to the COVID-19 vaccine in recent years were analyzed. This article reviews the phenomenon, preliminary mechanism, and related factors affecting weak vaccine response in patients with T2DM, which is expected to help in the development of new vaccines for high-risk groups for COVID-19.
作者机构：
[Hu, Linlin; Wei, Yanhong; Luo, Xiaoqiong; Liao, Meihua; Lu, Yiming; Wang, Junli] Reproductive Medicine, Guangxi Medical and Health Key Discipline Construction Project, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, China;[Wei, Yanhong; Lei, Xiaocan] Clinical Anatomy and Reproductive Medicine Application Institute, Hengyang Medical School, University of South China, Hengyang, 421001, China;[Lei, Xiaocan; Lu, Yiming; Wang, Junli] Industrial College of Biomedicine and Health Industry, Youjiang Medical University for Nationalities, Baise, 533000, China
摘要：
BACKGROUND: Due to changes in lifestyle and dietary habits, the global population with obesity is increasing gradually, resulting in a significant rise in the number of individuals having obesity. Obesity is caused by an imbalance between energy intake and consumption, leading to excessive fat accumulation, which interferes with normal human metabolism. It is also associated with cardiovascular disease, metabolic syndrome, male reproductive endocrine regulation disorders, systemic and local inflammatory reactions, excessive oxidative stress, and apoptosis. All these factors can damage the internal environment for sperm generation and maturation, resulting in male sexual dysfunction, a decline in sperm quality, and lower fertility. This study analyzes the trends and priorities of the effects of obesity on male reproductive disorders from a bibliometric perspective. METHODS: This study uses the Web of Science as the statistical source, covering all time spans. Tools like Web of Science, VOSviewer, and CiteSpace are used to analyze countries, institutions, authors, journals, and keywords in the field. Total publications, total citations, and average number of citations are selected for statistics. RESULTS: The results show that the research on the impact of obesity on male reproductive function can be roughly divided into three stages: the initial stage, the slow development stage, and the rapid development stage. Our statistical scope includes 463 highly relevant articles that we have screened. We found that the journal with the most publications in this field is Andrologia, and the institution with the highest total citations is the University of Utah. The most influential countries, institutions, and authors in this field are the United States, the University of Utah, and Carrell, Douglas. Currently, research related to the impact of obesity on male reproduction focuses mainly on three aspects: biochemistry, molecular biology, and reproductive biology. The keyword explosion results indicate that sperm, obesity, and male reproduction are at the forefront and trends of future research in this field. There has been a shift from basic biochemical and molecular research to research on molecular mechanisms relying on omics technologies. However, we have observed that the number of papers published in 2022 is lower than in 2021, indicating a growth interruption during this period. Considering that this deviation may be due to the impact of the COVID-19 pandemic, it may hinder the progress of certain experiments in 2022. In recent years, China has rapidly developed research in this field. However, the average citation rate is relatively low, indicating the need for Chinese scholars to improve the quality of their articles further. Based on our research and in the context of global obesity, men are at risk of increased infertility. Addressing this issue relies on our continued research into the mechanisms of obesity-related male reproductive disorders. Over the past forty-three years, with the contributions of scientists worldwide, research in this field has flourished. CONCLUSION: The impact of obesity on male reproductive disorders has been extensively studied. Currently, research in this field primarily focuses on male sperm function, sperm quality, and the effects or mechanisms of cells on male reproduction. Future trends in this field should concentrate on the relationship between male fertility and energy metabolism, as well as the endocrine function of adipose tissue. This study comprehensively analyzes the current research status and global trends in obesity and male reproductive disorders. We also discuss the future developments in this field, making it easier for researchers to understand its developmental history, current status, and trends, providing valuable reference for effective exploration in this area.
摘要：
This study aims to elucidate the effects of Platelet-rich plasma (PRP) in fibrosis development in intrauterine adhesion (IUA), and the associated underlying mechanisms are also explored, which are expected to be a potential therapeutic scheme for IUA. In this research, PRP was obtained and prepared from the peripheral venous blood of rats. A rat model was induced by mechanical injury. Further, PRP was directly injected into the uterus for treatment. The appearance and shape of the uterus were assessed based on the tissues harvested. The fibrosis biomarker levels were analyzed. The transforming growth factor beta 1 (TGF-β1) and Mothers against decapentaplegic homolog 7 (Smad7) levels, the phosphorylation of Smad2 (p-Smad2), and the phosphorylation of Smad3 (p-Smad3) were analyzed, and the molecular mechanism was investigated by rescue experiments. It was found that PRP improved the appearance and shape of the uterus in IUA and increased endometrial thickness and gland numbers. The administration of PRP resulted in a decrease in the expressions of fibrosis markers including collagen I, α-SMA, and fibronectin. Furthermore, PRP increased Smad7 levels and decreased TGF-β1 levels, p-Smad2, and p-Smad3. Meanwhile, administration of TGF-β1 activator reversed the therapeutic effects of PRP in IUA. Collectively, the intrauterine infusion of PRP can promote endometrial damage recovery and improve endometrial fibrosis via the TGF-β1/Smad pathway. Hence, PRP can be a potential therapeutic strategy for IUA.
