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Design and engineering of an artificial disulfide bond in human cytochrome c to regulate the protein structure and function

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成果类型:
期刊论文
作者:
Yan-Yan Li;Yu Feng;Lu Yu;Shuang-Shuang Long;Shu-Qin Gao;...
通讯作者:
Shuang-Shuang Long<&wdkj&>Ying-Wu Lin
作者机构:
School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China +86 743 8282375
[Lu Yu] High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, Anhui 230031, China
[Shu-Qin Gao] Key Lab of Protein Structure and Function of Universities in Hunan Province, University of South China, Hengyang 421001, China
[Yan-Yan Li; Yu Feng; Shuang-Shuang Long] School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China
[Ying-Wu Lin] School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China<&wdkj&>Key Lab of Protein Structure and Function of Universities in Hunan Province, University of South China, Hengyang 421001, China
通讯机构:
[Shuang-Shuang Long; Ying-Wu Lin] S
School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China<&wdkj&>Key Lab of Protein Structure and Function of Universities in Hunan Province, University of South China, Hengyang 421001, China<&wdkj&>School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China
语种:
英文
期刊:
Molecular Systems Design & Engineering
ISSN:
2058-9689
年:
2024
机构署名:
本校为第一且通讯机构
院系归属:
化学化工学院
摘要:
Human cytochrome c (hCyt c) contains a covalently attached heme group with six-coordination (Met/His) and plays vital biological functions, including electron transfer and peroxidase activity by structural alterations, as well as other functions by interactions with partners such as neuroglobin (Ngb). In this study, we designed and engineered an artificial disulfide bond in hCyt c via double mutations (A51C/G77C) which bridges the Ω-loops C and D. The formation of the intramolecular disulfide bond (Cys51–Cys77) was confirmed by mass spectrome...

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