Sortilin调控ABCA1蛋白水平影响荷脂巨噬细胞内脂质流出
作者:
钟丽园;张明鑫;吕运成;陶水英;胡杨;...
期刊:
中国临床解剖学杂志 ,2020年38(5):568-573 ISSN:1001-165X
作者机构:
南华大学肿瘤研究所湖南省肿瘤细胞与分子病理学重点实验室湖南省胃癌研究中心,湖南衡阳421000;南华大学衡阳医学院应用解剖与生殖医学研究所,湖南衡阳421000;[胡杨; 陶水英; 吕运成; 彭田红; 王茜; 张明鑫; 钟丽园; 唐志桦] 南华大学
关键词:
巨噬细胞;胆固醇流出
摘要:
目的探讨Sortilin对荷脂THP-1巨噬细胞ABCA1蛋白表达及胞内脂质流出的影响。方法采用Western blot和qRT-PCR检测细胞内ABCA1蛋白和mRNA表达水平;Co-IP实验检测Sortilin和ABCA1的结合情况;高效液相色谱法检测细胞内总胆固醇、游离胆固醇、胆固醇酯含量变化;油红O染色观察细胞内脂滴情况。结果 Sortilin下调细胞内ABCA1蛋白水平,但对其mRNA水平无显著影响;Co-IP结果表明Sortilin能与ABCA1结合;与对照组相比,Sortilin过表达后荷脂巨噬细胞内胆固醇流出减少,胞内脂质含量增加且脂滴肥大,数量明显增多,Sortilin沉默后荷脂巨噬细胞胆固醇流出增加,胞内脂质含量减少,脂滴瘦小,数量减少;溶酶体抑制剂氯喹共处理可部分逆转Sortilin过表达对巨噬细胞ABCA1蛋白和胆固醇流出的抑制作用。结论 Sortilin下调巨噬细胞ABCA1蛋白水平,抑制胆固醇流出,促进胞内脂质蓄积。
语种:
中文
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关于提高医学留学生解剖学实验课教学效果的探讨
作者:
吕运成;陈安英;孙莎;陈胜华;陈熙;...
期刊:
教育现代化 ,2019年(09):33-35 ISSN:2095-8420
作者机构:
1. 南华大学衡阳医学院解剖学教研室;2. 南华大学语言文学学院第一外语教研室
关键词:
留学生教育;解剖实验课;教学改革;教学效果
摘要:
为提高医学留学生解剖学实验教学效果,从加强教师素质培养、改革教学模式和实验室建设三方面入手,全面提高留学生解剖实验课的学习兴趣和实践动手能力,培养适应国际化竞争需求的医药卫生人才。
语种:
中文
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浅谈如何充分发挥人体标本在医学留学生解剖实验教学中的作用
作者:
吕运成;陈安英;孙莎;彭田红;谭建国;...
期刊:
教育现代化 ,2019年(18):156-158 ISSN:2095-8420
作者机构:
1. 南华大学衡阳医学院解剖学教研室;2. 南华大学语言文学学院第一外语教研室;3. 南华大学衡阳医学院生理学教研室
关键词:
人体标本;解剖实验教学;动手能力培养;医学留学生教育
摘要:
人体标本在医学留学生解剖实验教学中发挥着重要作用,本文从加强实验室标本建设,提高教师素质和改革课程考核体制三方面展开讨论,以期充分发挥人体标本在医学留学生解剖实验教学中的作用,激发留学生对解剖实验课的兴趣,提高留学生解剖学实验课的学习效率。
语种:
中文
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浅谈如何科学合理地规划留学生局部解剖学的标本操作
作者:
吕运成;欧阳曙晖;孙莎;彭田红;刘政海;...
期刊:
教育现代化 ,2019年(16):161-163 ISSN:2095-8420
作者机构:
1. 南华大学医学院解剖学教研室;2. 南华大学语言文学学院第一外语教研室
关键词:
局部解剖学;标本操作;医学留学生教育
摘要:
为了科学合理地规划留学生标本操作,通过加强对留学生的筛选、加强标本的收集与管理、改善实验室环境、加快学校数字化信息教学的建设、合理安排实验课时、努力提高教师业务素质等措施,切实提高留学生解剖操作的科学性与合理性。
语种:
中文
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EIF2α promotes vascular remodeling via autophagy in monocrotaline-induced pulmonary arterial hypertension rats
作者:
Guo, Linya;Li, Yanbing;Tian, Ying;Gong, Shaoxin;Chen, Xi;...
期刊:
Drug Design, Development and Therapy ,2019年13:2799-2809 ISSN:1177-8881
通讯作者:
Jiang, Zhisheng;Wang, Aiping
作者机构:
[Guo, Linya; Chen, Xi; Wang, Aiping; Peng, Tianhong] Univ South China, Sch Med, Clin Anat & Reprod Med Applicat Inst, Hengyang 421001, Peoples R China.;[Li, Yanbing] Southern Med Univ, Natl Key Discipline Human Anat, Guangzhou 510000, Guangdong, Peoples R China.;[Li, Yanbing] Guangdong Engn Res Ctr Translat Med 3D Printing A, Guangzhou 510000, Guangdong, Peoples R China.;[Wang, Aiping; Tian, Ying] Univ South China, Affiliated Nanhua Hosp, Inst Clin Res, Hengyang 421002, Hunan, Peoples R China.;[Wang, Aiping; Tian, Ying] Univ South China, Postdoctoral Res Inst Basic Med, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Jiang, Zhisheng; Wang, Aiping] U;Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Nanhua Hosp, Inst Clin Res, Hengyang 421001, Hunan, Peoples R China.
关键词:
eIF2α;autophagy;PASMCs;PAH;monocrotaline
摘要:
Purpose: Eukaryotic initiation factor 2α (eIF2α) plays important roles in the proliferation and survival of pulmonary artery smooth muscle cells (PASMCs) in animal hypoxia-induced pulmonary hypertension models. However, the underlying mechanism remains unknown at large. Autophagy has been reported to play a key role in the vascular remodeling in pulmonary arterial hypertension (PAH). The purposes of this study are to determine the functions of eIF2α and autophagy in the vascular remodeling of the monocrotaline-induced PAH rats and to clarify the correlation between eIF2α and autophagy. Methods: We established a rat model of monocrotaline-induced PAH, and we established a cell model of platelet derived growth factor (PDGF)-induced PASMCs proliferation. The vascular morphology and the expression of eIF2α, LC3B, and p62 were assessed in the pulmonary arterial tissue of Sprague-Dawleyrats and PDGF-induced PASMCs. Results: Autophagy was significantly active in monocrotaline model group (MCT)-induced PAH rats, which obviously promotes vascular remodeling in MCT-induced PAH rats. Furthermore, the proliferation of PASMCs was induced by PDGF in vitro. The expression of LC3B, eIF2α was increased in the PDGF-induced PASMCs proliferation, and the expression of p62 was reduced in the PDGF-induced PASMCs proliferation. Moreover, eIF2α siRNA downregulated the expression of eIF2α and LC3B, and upregulated the expression of p62 in PDGF-induced PASMCs proliferation. eIF2α siRNA inhibited the PDGF-induced PASMCs proliferation. Finally, chloroquine can upregulate the protein expression of LC3B and p62, it also can inhibit proliferation in PDGF-induced PASMCs. Conclusion: Based on these observations, we conclude that eIF2α promotes the proliferation of PASMCs and vascular remodeling in monocrotaline-induced PAH rats through accelerating autophagy pathway. © 2019 Guo et al.
语种:
英文
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整合医学理念下医学创新人才培养的初步探索
作者:
吕运成;钟丽园;王爱平;陈胜华;李彩;...
期刊:
高教学刊 ,2019年(8):33-34,37 ISSN:2096-000X
作者机构:
南华大学 衡阳医学院 解剖学教研室,湖南 衡阳,421001;[王爱平; 吕运成; 彭田红; 李彩; 陈胜华; 高安博; 钟丽园; 陈熙] 南华大学
关键词:
整合医学;医学创新人才;医学教学改革
摘要:
医学创新人才培养是医学教育的核心内容。通过对医学创新人才内涵的阐述,分析创新人才培养的现状和基本问题;以整合医学理念为向导,探索医学创新人才培养的有效途径,即树立创新型教学理念、应用整合型创新教学模式、优化创新人才综合考核制度。
语种:
中文
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第1及第2骶椎骶髂螺钉置钉的基础研究与临床应用进展
作者:
刘方;周子嘉;王爱平;彭田红;郭林亚;...
期刊:
中国临床解剖学杂志 ,2018年36(1):111-113 ISSN:1001-165X
作者机构:
[刘方; 周子嘉; 王爱平; 彭田红; 郭林亚; 李严兵] 南华大学医学院解剖学教研室,医学院临床应用解剖研究室, 湖南, 衡阳, 421001
关键词:
骶椎;骶髂;螺钉
摘要:
如何通过腰骶固定融合手术实现稳定的骨性融合一直是脊柱外科面临的难题。各种融合至骶骨的骨盆固定技术已广泛应用,它们在稳固腰骶部,提高融合率,纠正脊柱畸形、骨盆倾斜及减少假性关节和并发症发生等方面起到至关重要的作用。随着腰骶椎疾病发病率逐年上升和患者对生活质量要求的不断提高,传统的骶骨骨盆固定技术如Galveston棒固定、髂骨螺钉固定及S_1或/和S_2螺钉内固定已不能满足临床科学技术发展的需要。2007年Sponseller提出了经第2骶椎骶髂(second sacral ala-iliac,S_2AI)螺钉固定新技术,此后报道了第1骶椎骶髂(first sacral ala-iliac,S_1AI)螺钉固定。由于此两种螺钉固定术具有良好的生物力学性能、无需交叉连接器、易于经皮或微创手术放置、并发症较少以及不妨碍髂骨翼植骨取材等优点,已越来越多地成为取代骶骨骨盆固定术的优先选择。然而,由于S_1AI、S_2AI钉道较狭长,骶骨骨质薄弱,腰骶部需承受较大的载荷以及腰骶角引起的局部剪切应力等因素,以致螺钉置入相对困难,且易发生固定融合失败;加之术后假关节形成,内置物松动或断裂等并发症时有发生,致使S_1AI、S_2AI螺钉置钉尚难达到理想的状态。因此,许多学者进行了大量相关的基础和临床应用研究。现综述如下。
语种:
中文
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“互联网+”背景下人体解剖学专业硕士研究生培养新模式的思考与探索
作者:
高安博;钟丽园;吕运成;陈胜华;彭田红
期刊:
教育现代化 ,2018年5(01):1-3 ISSN:2095-8420
作者机构:
南华大学医学院应用解剖与生殖医学研究所,湖南 衡阳,421001;[吕运成; 彭田红; 陈胜华; 高安博; 钟丽园] 南华大学
关键词:
互联网+;人体解剖学;硕士研究生;教学改革
摘要:
"互联网+"模式是医学高等教育改革发展的必然趋势,将成为人体解剖学专业研究生培养新模式的必然选择.本文旨在以"互联网+"为契机,从我校人体解剖学专业研究生的培养现状出发,探讨解剖学专业研究生的培养新思路,以"互联网+"硬件配置和软件建设为手段,通过逐渐完善"互联网+"评价制度,最终构建基于"互联网+"背景下解剖学专业研究生培养的新模式,引领人体解剖学专业研究生教学改革新方向.
语种:
中文
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核因子κB通过Sortilin促进荷脂THP-1巨噬细胞泡沫化
作者:
高安博;彭田红;钟丽园;孙莎;陈熙;...
期刊:
中国动脉硬化杂志 ,2018年26(7):666-671 ISSN:1007-3949
作者机构:
南华大学医学院应用解剖与生殖医学研究所,湖南省衡阳市,421001;[李素云; 万炜; 吕运成; 彭田红; 高安博; 谢巍; 钟丽园; 陈熙; 孙莎] 南华大学
关键词:
核因子κB;巨噬细胞;脂质蓄积;泡沫化
摘要:
目的探究核因子κB(NF-κB)是否通过分拣蛋白Sortilin影响荷脂THP-1巨噬细胞脂质代谢和泡沫化。方法 NF-κB激活剂PMA或其特异性抑制剂PDTC孵育荷脂THP-1巨噬细胞,qRT-PCR检测Sortilin mRNA水平,Western blot检测Sortilin蛋白表达;沉默荷脂THP-1巨噬细胞Sortilin同时加入PMA孵育,胞内胆固醇流出行液体闪烁计数器检测,胞内脂质含量行高效液相色谱法检测,胞内脂滴情况用油红O染色显示。结果 PMA孵育处理荷脂THP-1巨噬细胞后Sortilin mRNA水平及蛋白表达上调,PDTC孵育处理后Sortilin mRNA水平及蛋白表达下调;PMA孵育处理荷脂THP-1巨噬细胞内脂质流出减少,脂质蓄积程度增加,泡沫细胞形成加剧,而PMA与Sortilin shRNA共处理后,其胞内脂质流出增多,胞内脂质蓄积程度减轻,泡沫细胞形成受抑。结论 NF-κB通过促进Sortilin表达,导致巨噬细胞内脂质流出受抑,胞内脂质蓄积程度加重,从而加剧泡沫细胞形成。
语种:
中文
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The zonal pattern of arterial supply to the brachial plexus and its clinical significance
作者:
Gao, An-bo;Lv, Yun-cheng;Wang, Ai-ping;Zhong, Li-yuan;Tang, Mao-lin;...
期刊:
SURGICAL AND RADIOLOGIC ANATOMY ,2018年40(7):815-822 ISSN:0930-1038
通讯作者:
Peng, Tian-hong;Thomas, Binu-prathap
作者机构:
[Wang, Ai-ping; Lv, Yun-cheng; Gao, An-bo; Zhong, Li-yuan; Peng, Tian-hong] Univ South China, Sch Med, Clin Anat & Reprod Med Applicat Inst, Hengyang 421001, Peoples R China.;[Thomas, Binu-prathap] Christian Med Coll & Hosp, Dr Paul Brand Ctr Hand Surg & Peripheral Nerve Su, Ida Scudder Rd, Vellore 632004, Tamil Nadu, India.;[Tang, Mao-lin] Wenzhou Med Univ, Dept Anat, Wenzhou 325035, Peoples R China.
通讯机构:
[Peng, Tian-hong] U;[Thomas, Binu-prathap] C;Univ South China, Sch Med, Clin Anat & Reprod Med Applicat Inst, Hengyang 421001, Peoples R China.;Christian Med Coll & Hosp, Dr Paul Brand Ctr Hand Surg & Peripheral Nerve Su, Ida Scudder Rd, Vellore 632004, Tamil Nadu, India.
关键词:
Brachial plexus;Subclavian artery;Axillary artery;Microsurgical techniques
摘要:
Purpose: To provide the anatomical basis of blood supply of brachial plexus for the clinical microsurgical treatment of brachial plexus injury. Methods: Thirteen adult anticorrosive cadaveric specimens (8 males, 5 females) were dissected in this study. 3 fresh cases (2 males, 1 female) were used to observe the zonal pattern of arteries supplying brachial plexus, and 10 cases (6 males, 4 females) were used to observe the source and distribution of the brachial plexus arteries under microscope. Results: The brachial plexus is supplied by branches of the subclavian–axillary axis (SAA), and these branches anastomose each other. According to distribution feature, blood supply of the brachial plexus could be divided into three zones. The first zone was from the nerve roots of intervertebral foramina to its proximal trunks, which was supplied by the vertebral artery and the deep cervical artery. The second zone was from the distal nerve trunks of the brachial plexus, encompassing the divisions to its proximal cords, which was supplied by direct branches of the subclavian artery or by branches originating from the dorsal scapular artery. The third zone was from the distal portion of the cords to terminal branches of the brachial plexus, which was supplied by direct branches of the axillary artery. Conclusions: The zonal pattern of arterial supply to the brachial plexus is a systematic and comprehensive modality to improve anatomical basis for the clinical microsurgical treatment for brachial plexus injury. © 2018, Springer-Verlag France SAS, part of Springer Nature.
语种:
英文
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分拣受体Sortilin促进巨噬细胞内脂质蓄积
作者:
钟丽园;彭田红;高安博;万炜;陈熙;...
期刊:
中国动脉硬化杂志 ,2018年26(2):139-143 ISSN:1007-3949
作者机构:
南华大学医学院应用解剖与生殖医学研究所,湖南省衡阳市,421001;南华大学医学院心血管疾病研究所,湖南省衡阳市,421001;[王莉; 万炜; 唐志晗; 吕运成; 王佐; 彭田红; 高安博; 钟丽园; 陈熙; 欧阳宇甲] 南华大学
关键词:
巨噬细胞;脂质蓄积;氧化型低密度脂蛋白;动脉粥样硬化
摘要:
目的 探讨分拣受体Sortilin对THP-1巨噬细胞内脂质代谢的影响.方法 用氧化型低密度脂蛋白(ox-LDL)孵育THP-1巨噬细胞.Western blot检测荷脂后THP-1巨噬细胞中Sortilin表达变化;THP-1巨噬细胞Sortilin高表达和沉默后,液体闪烁计数仪检测胞内胆固醇流出,高效液相色谱法检测细胞内脂质含量,油红O染色观察胞内脂滴情况.结果 THP-1巨噬细胞Sortilin表达呈ox-LDL剂量和时间依赖性增加;以50 mg/L ox-LDL处理24 h后THP-1巨噬细胞Sortilin表达水平达到高峰.与对照组相比,Sortilin过表达后巨噬细胞胆固醇流出减少,胞内脂质含量增加且脂滴明显增多,而Sortilin沉默后则刚好出现相反的结果.结论 Sortilin抑制巨噬细胞内胆固醇流出,促进胞内脂质蓄积.
语种:
中文
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Sortilin promotes macrophage cholesterol accumulation and aortic atherosclerosis through lysosomal degradation of ATP-binding cassette transporter A1 protein
作者:
Lv, Yuncheng;Yang, Jing;Gao, Anbo;Sun, Sha;Zheng, Xilong;...
期刊:
生物化学与生物物理学报 ,2018年51(5):471-483 ISSN:1672-9145
通讯作者:
Peng, Tianhong;Zhong, Liyuan;Zhao, Guojun
作者机构:
[Chen, Xi; Sun, Sha; Lv, Yuncheng; Wan, Wei; Gao, Anbo; Xie, Wei; Peng, Tianhong; Guo, Dongming; Zhong, Liyuan; Li, Suyun; Peng, TH] Univ South China, Clin Anat & Reprod Med Applicat Inst, Hengyang 421001, Peoples R China.;[Yang, Jing] Univ South China, Affiliated Hosp 1, Clin Med Res Inst, Hengyang 421001, Peoples R China.;[Zheng, Xilong] Univ Calgary, Libin Cardiovasc Inst Alberta, Dept Biochem & Mol Biol, Hlth Sci Ctr, Calgary T2N 4N1, AB, Canada.;[Tang, Chaoke] Univ South China, Inst Cardiovasc Res, Med Res Ctr, Hengyang 421001, Peoples R China.;[Zhao, Guojun] Guilin Med Univ, Dept Histol & Embryol, Guilin 541004, Peoples R China.
通讯机构:
[Peng, TH; Zhong, LY] U;[Zhao, Guojun] G;Univ South China, Clin Anat & Reprod Med Applicat Inst, Hengyang 421001, Peoples R China.;Guilin Med Univ, Dept Histol & Embryol, Guilin 541004, Peoples R China.
关键词:
sortilin;ABCA1;macrophage;cholesterol efflux;atherosclerosis
摘要:
Sortilin is closely associated with hyperlipidemia and the risk of atherosclerosis (AS). The role of sortilin and the underlying mechanism in peripheral macrophage are not fully understood. In this study, we investigated the effect of macrophage sortilin on ATP-binding cassette transporter A1 (ABCA1) expression, ABCA1-mediated cholesterol efflux, and aortic AS. Macrophage sortilin expression was upregulated by oxidized low-density lipoproteins (ox-LDLs) in both concentrationand time-dependent manners. Its expression reached the peak level when cells were incubated with 50 ?g/ml ox-LDL for 24 h. Overexpression of sortilin in macrophage reduced cholesterol efflux, leading to an increase in intracellular total cholesterol, free cholesterol, and cholesterol ester. Sortilinwas found to bind with ABCA1 protein and suppress macrophage ABCA1 expression, resulting in a decrease in cholesterol efflux from macrophages. The inhibitory effect of sortilin in cholesterol efflux was partially reversed by treatment with chloroquine, a lysosomal inhibitor. On the contrary, the ABCA1 protein level and ABCA1-mediated cholesterol efflux is increased by sortilin short hairpin RNA transfection. The fecal and biliary cholesterol 3H-sterol from cholesterolladen mouse peritoneal macrophage was reduced by sortilin overexpression through lentivirus vector (LV)-sortilin in low-density lipoprotein receptor knockout mice, which was prevented by cotreatment with chloroquine. Treatment with LV-sortilin reduced plasma high-density lipoprotein and increased plasma ox-LDL levels. Accordingly, aortic lipid deposition and plaque area were exacerbated, and ABCA1 expression was reduced in mice in response to infection with LVsortilin alone. These effects of LV-sortilin were partially reversed by chloroquine. Sortilin enhances lysosomal degradation of ABCA1 protein and suppresses ABCA1-mediated cholesterol efflux from macrophages, leading to foam cell formation and AS development. © 2019 The Author(s) .
语种:
英文
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基础与临床全方位相结合的护理专业系统解剖学教学模式探讨
作者:
王爱平;唐谟建;李严兵;陈胜华;彭田红;...
期刊:
山西医科大学学报 ,2017年19(3):173-175 ISSN:1007-6611
作者机构:
南华大学医学院解剖学教研室,衡阳,421001;[王爱平; 彭田红; 陈胜华; 谭建国; 李严兵; 陈熙; 唐谟建] 南华大学
关键词:
系统解剖学;基础与临床;教学改革;护理专业
摘要:
文章就护理专业基础与临床全方位相结合的系统解剖学教学模式进行论述,围绕护理专业系统解剖学教学为何要将基础与临床全方位相结合以及如何做到这一点作了详细探讨。通过精选教材内容、PBL教学模式、体表标志的触摸、实验教学学生自主观察并课后绘图以及课后反馈等方式可以使系统解剖学与临床护理全方位地结合在一起。相比传统护理专业系统解剖学教学模式,新的教学模式教学重点突出、学生参与积极性高涨、学生掌握知识结构更完整,对于护理专业学生学好系统解剖学并提高其自学能力、实践能力和创新能力具有重要作用。
语种:
中文
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医学人文教育在解剖学教学中的应用
作者:
徐皓南;王爱平;安高;彭田红;徐菁;...
期刊:
教育现代化 ,2017年(11):102-103 ISSN:2095-8420
作者机构:
南华大学 医学院解剖学教研室,湖南 衡阳,421001;[王爱平; 徐菁; 彭田红; 徐皓南; 陈熙; 安高] 南华大学
关键词:
医学人文教育;解剖学教学;应用
摘要:
解剖学是医学重要的基础学科,是涉及生命体的结构和组织的生物学分支学.医学人文教育与解剖学教学相结合,将会有力地变革现有的解剖学教学氛围,创造崭新的教学体系,呼应教改大潮,提高医学生的人文素养,为将来的国家培养一批有能力,更有情感的合格医生.
语种:
中文
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7-二氟亚甲基-5,4’-二甲氧基染料木黄酮通过下调Caspase-3的表达拮抗H2O2诱导的血管内皮细胞凋亡
作者:
王莉;李素云;彭田红;何慧;吕运成;...
期刊:
中国动脉硬化杂志 ,2017年25(12):1219-1224 ISSN:1007-3949
作者机构:
南华大学医学院解剖学教研室,湖南省衡阳市,421001;湖南师范大学医学院,湖南省长沙市,410013;[王莉; 李素云; 吕运成; 彭田红; 何慧] 南华大学;[符晓华; 曹建国] 湖南师范大学
关键词:
7-二氟亚甲基-5,4’-二甲氧基染料木黄酮;过氧化氢;细胞凋亡
摘要:
目的 研究7-二氟亚甲基-5,4'-二甲氧基染料木黄酮对H2O2诱导血管内皮细胞凋亡的影响,并初步探讨其作用机制.方法 用1 mmoL/L H2O2诱导人脐静脉内皮细胞建立氧化应激损伤模型.实验分为空白对照组、H2O2损伤组、溶媒对照组、先导化合物组(100 mol/L染料木黄酮)、不同浓度(0.1、0.3、1、3、10 μmol/L) 7-二氟亚甲基-5,4'-二甲氧基染料木黄酮组.DCFH-DA激活荧光流式细胞术测定活性氧的生成,吖啶橙染色荧光显微镜观察细胞凋亡形态,PI染色流式细胞术检测细胞凋亡率,Western blot测定Caspase-3的表达.结果 1 mmol/LH2O2孵育血管内皮细胞24h,活性氧的生成显著增加,吖啶橙染色荧光显微镜下细胞呈现明显的凋亡形态学改变,细胞凋亡率升高,Caspase-3的表达上调.用7-二氟亚甲基-5,4'-二甲氧基染料木黄酮预处理血管内皮细胞后可见活性氧的生成减少,细胞凋亡率降低,Caspase-3的表达下调.结论 7-二氟亚甲基-5,4'-二甲氧基染料木黄酮对H2O2诱导血管内皮细胞凋亡有明显的抑制作用,其作用机制可能是通过降低活性氧的生成,下调Caspase-3的表达,从而拮抗H2O2诱导的血管内皮细胞凋亡.
语种:
中文
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Implications of sortilin in lipid metabolism and lipid disorder diseases
作者:
Gao, Anbo;Cayabyab, Francisco S.;Chen, Xi;Yang, Jing;Wang, Li;...
期刊:
DNA AND CELL BIOLOGY ,2017年36(12):1050-1061 ISSN:1044-5498
通讯作者:
Peng, Tianhong;Lv, Yuncheng
作者机构:
[Chen, Xi; Wang, Li; Gao, Anbo; Peng, Tianhong; Lv, Yuncheng; Peng, TH; Lv, YC] Univ South China, Clin Anat & Reprod Med Applicat Inst, Hengyang 421001, Peoples R China.;[Cayabyab, Francisco S.; Lv, Yuncheng] Univ Saskatchewan, Dept Surg, Coll Med, Saskatoon, SK, Canada.;[Yang, Jing] Univ South China, Affiliated Hosp 1, Dept Endocrinol & Metab, Hengyang, Peoples R China.
通讯机构:
[Peng, TH; Lv, YC] U;Univ South China, Clin Anat & Reprod Med Applicat Inst, Hengyang 421001, Peoples R China.
关键词:
atherosclerosis;lipid metabolism;nonalcoholic fatty liver disease;sortilin;type 2 diabetes mellitus
摘要:
The present review provides a summary of recent evidence of sortilin expression, function, and regulation and its implications in lipid metabolism and development of lipid disorder diseases. As a member of the vacuolar protein sorting 10 protein (Vps10p) receptor family, sortilin mediates intracellular trafficking of diverse endogenous or exogenous protein substrates between the trans-Golgi network (TGN) and plasma membrane compartments. Recent studies reveal that sortilin regulates the expression of lipid genes, plasma lipid level, and the development of lipid disorder diseases. Sortilin promotes atherogenesis by regulating hepatic very low density lipoprotein (VLDL) secretion and plasma lipid level and subsequently macrophage lipid accumulation. Sortilin deficiency is caused by accelerated proteasome degradation under insulin resistance conditions and is thereby implicated in the hyperlipidemia of type 2 diabetes mellitus (T2DM). Sortilin facilitates hepatic cholesterol accumulation by inhibiting hepatic cholesterol catabolism, which promotes the development of nonalcoholic fatty liver disease (NAFLD). Sortilin plays an important role in lipid metabolism and represents a promising therapeutic target for lipid disorder diseases.
语种:
英文
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Microanatomy of the brachial plexus roots and its clinical significance
作者:
Zhong, Li-yuan;Wang, Ai-ping;Hong, Li;Chen, Sheng-hua;Wang, Xian-qin;...
期刊:
SURGICAL AND RADIOLOGIC ANATOMY ,2017年39(6):601-610 ISSN:0930-1038
通讯作者:
Lv, Yun-cheng;Peng, Tian-hong
作者机构:
[Wang, Ai-ping; Lv, Yun-cheng; Wang, Xian-qin; Zhong, Li-yuan; Peng, Tian-hong; Lv, YC; Peng, TH; Hong, Li; Chen, Sheng-hua] Univ South China, Lab Clin Anat, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Lv, YC; Peng, TH] U;Univ South China, Lab Clin Anat, Hengyang 421001, Hunan, Peoples R China.
关键词:
Roots of the brachial plexus;Avulsion of the brachial plexus;Microanatomy;Blood supply of the brachial plexus
摘要:
To provide the anatomical basis of brachial plexus roots for the diagnosis and treatment of brachial plexus root avulsion injury. The morphological features of brachial plexus roots were observed and measured on 15 cervicothoracic spine of adult cadavers. The relationship of brachial plexus nerve roots and the surrounding tissues also were observed, as well as the blood supply of anterior and posterior roots of the brachial plexus. Origination of the nerve roots in the dorsal–ventral direction from the midline was fine-tuned at each level along the spinal cord. The minimum distance of the origin of the nerve root to midline was 2.2 mm at C
5, while the maximum was 3.1 mm at T
1. Inversely, the distance between the origin of the posterior root and the midline of the spinal cord gradually decreased, the maximum being 4.2 mm at C
5 and minimum 2.7 mm at T
1. Meanwhile, there was complicated fibrous connection among posterior roots of the brachial plexus. The C
5–6 nerve roots interlaced with tendons of the scalenus anterior and scalenus medius and fused with the transverse-radicular ligaments in the intervertebral foramina. However, these ligaments were not seen in C
7–8, and T
1. The blood supply of the anterior and posterior roots of the brachial plexus was from the segmental branches of the vertebral artery, deep cervical artery and ascending cervical artery, with a mean outer diameter of 0.61 mm. The systematic and comprehensive anatomic data of the brachial plexus roots provides the anatomical basis to diagnose and treat the brachial plexus root avulsion injury.
语种:
英文
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构建人体解剖学实验开放平台培养创新人才
作者:
王爱平;李严兵;周小兵;谢巍;彭田红;...
期刊:
教育教学论坛 ,2016年(08):264-266 ISSN:1674-9324
作者机构:
南华大学医学院人体解剖学教研室,湖南衡阳,421001;南华大学医学院,湖南衡阳,421001;[王爱平; 彭田红; 谢巍; 周小兵; 李严兵; 田英] 南华大学
关键词:
人体解剖学;开放实践;平台构建;创新人才
摘要:
人体解剖学是医学教育中的重要学科,是医学各学科的基础。解剖学的学习效果对进一步学习其他课程及今后的临床实践起着非常重要的作用,而实验教学担负着提升医学生动手能力及创新意识的关键作用。研究表明,综合院校构建开放实验室是提高医学生的创新和实践能力的有效途径。因此,构建人体解剖学实验开放平台能够使人体解剖学实验教学与培养医学生综合科研素养及创新能力紧密结合,旨在增强学生的主体地位、实践创新能力、科学探索精神及团队合作意识均具有非常重要的现实意义。
语种:
中文
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三维数字化模型在人体解剖学教学中应用的探讨
作者:
李美秀立;彭田红;曹妍群;黄铠;易志勇;...
期刊:
Kexue Jishu Chuangxin ,2016年(32):107 ISSN:1673-1328
作者机构:
邵阳学院基础课部人体解剖与组织胚胎学教研室,湖南邵阳,422000;南华大学医学院人体解剖学教研室,湖南衡阳,418000;[曹妍群; 黄铠; 易志勇; 李美秀立; 郭兴; 陆曲折] 邵阳学院;[彭田红] 南华大学
关键词:
三维数字化模型;人体解剖
摘要:
人体解剖学一直是学生学习的重点和难点。基于人体解剖学的教学现状,对比了传统的教学模式与三维数字化模型在人体解剖学教学中应用的优劣性。论述了将三维重建数字化模型应用到人体解剖学教学实践中的必要性。
语种:
中文
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双通道定位与盲分离结合的噪声分离方法
作者:
彭珍山;彭田红;肖建忠;陈胜华;谭建国
期刊:
噪声与振动控制 ,2016年36(3): 137-141,163 ISSN:1006-1355
作者机构:
[彭珍山; 彭田红; 肖建忠; 陈胜华; 谭建国] 南华大学医学院解剖学教研室
关键词:
声学;双通道定位;延时;衰减;盲源分离;噪声分离
摘要:
探索双通道定位算法与盲源分离相结合进行噪声分离的可能。根据双通道空间定位理论,以声音传播过程中在双通道间产生的延时差和强度差为线索对混合信号进行分离。当待分离的辐射噪声中存在时频混叠或同一位置多个源时,双通道定位分离算法能分离出不同位置处源信号,然后利用盲源分离方法对同一位置处的多个源信号进行再分离。在Roomsim中进行分离方案的可行性验证,假设房间六个壁能100%吸收所有频率信号,不存在反射与混响,对比分析声源位置及个数对分离结果的影响。结果表明,当源位置的个数不大于3时,双通道定位分离算法能有效分离出不同位置的声源信号,用盲源分离方法可进一步分离出相同位置处的不同源信号,但当源位置个数大于等于4时双通道定位分离算法的分离性能降低。
语种:
中文
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