Nanoparticle-modified chitosan-agarose-gelatin scaffold for sustained release of SDF-1 and BMP-2
作者：
Wang, Bin;Guo, Yuanwei;Chen, Xiaofeng;Zeng, Chao;Hu, Qikang;...
期刊：
INTERNATIONAL JOURNAL OF NANOMEDICINE ,2018年13:7395-7408 ISSN：1178-2013
通讯作者：
Yu, Fenglei
作者机构：
[Hu, Qikang; Zeng, Chao; Li, Wei; Huang, Xingchun; Zhang, Bingyu; Yin, Wei; Xie, Hui; Wang, Bin; Yu, Fenglei] Cent S Univ, Xiangya Hosp 2, Dept Thorac Surg, 139 Renmin Rd, Changsha 410011, Hunan, Peoples R China.;[Guo, Yuanwei] Univ South China, Peoples Hosp Chenzhou 1, Ctr Clin Pathol, Chenzhou 432000, Peoples R China.;[Chen, Xiaofeng] Cent S Univ, Xiangya Hosp 2, Dept Anesthesiol, Changsha 410011, Hunan, Peoples R China.
通讯机构：
[Yu, Fenglei] C;Cent S Univ, Xiangya Hosp 2, Dept Thorac Surg, 139 Renmin Rd, Changsha 410011, Hunan, Peoples R China.
关键词：
cytokine delivery system;nanoparticles;chitosan-agarose-gelatin scaffold;stromal cell-derived factor-1;bone morphogenetic protein-2
摘要：
Bin Wang,1 Yuanwei Guo,2 Xiaofeng Chen,3 Chao Zeng,1 Qikang Hu,1 Wei Yin,1 Wei Li,1 Hui Xie,1 Bingyu Zhang,1 Xingchun Huang,1 Fenglei Yu1 1Department of Thoracic Surgery, The Second Xiangya Hospital of Central South University, Changsha 410011, People’s Republic of China; 2Center for Clinical Pathology, Affiliated to The First People’s Hospital of Chenzhou, University of South China, Chenzhou 432000, People’s Republic of China; 3Department of Anesthesiology, The Second Xiangya Hospital of Central South University, Changsha 410011, People’s Republic of China Background: Stromal cell-derived factor 1 (SDF-1) is an important chemokine for stem cell mobilization, and plays a critical role in mobilization of mesenchymal stem cells (MSCs). Bone morphogenetic protein 2 (BMP-2) plays a critical role in osteogenesis of MSCs. However, the use of SDF-1 and BMP-2 in bone tissue engineering is limited by their short half-lives and rapid degradation in vitro and in vivo. Methods: The chitosan oligosaccharide/ heparin nanoparticles (CSO/H NPs) were first prepared via self-assembly. Chitosan-agarose-gelatin (CAG) Scaffolds were then synthesized via gelation technology using cross-linked chitosan, agarose, and gelatin, and were modified by CSO/H NPs. The encapsulation efficiency and release kinetics of SDF-1 and BMP-2 were quantified using an enzyme-linked immunosorbent assay. A CCK-8 assays were used to evaluate biocompatibility of NP-modified scaffolds. The biological activity of the loaded SDF-1 and BMP-2 was evaluated using the transwell migration assay and osteogenic induction assay. An animal MSC recruitment model was used to study the ability of SDF-1 released from NP-modified scaffolds to induce migration of MSCs. Results: In this study, we developed a novel nanoparticle-modified CAG scaffold for the delivery of SDF-1 and BMP-2. CCK-8 assays demonstrated excellent biocompatibility of NP-modified scaffolds. In addition, we investigated the release of SDF-1 and BMP-2 from NP-modified scaffolds, and evaluated the effect of released SDF-1 on MSC migration. The effect of released BMP-2 on MSC osteogenesis was also examined. In vitro cell migration assays showed that SDF-1 released from NP-modified scaffolds retained its migration activity; osteogenesis studies demonstrated that released BMP-2 exhibited a strong ability to induce differentiation towards osteoblasts. Our in vivo recruitment assays showed continuous chemotactic response of MSCs to SDF-1 released from the NP-modified scaffold.Conclusion: The simplicity of synthesizing CSO/H NP-modified CAG scaffolds, combined with its high cytokine loading capacity and sustained release effect, renders NP-modified CAG scaffold an attractive candidate for sustained release of SDF-1 and BMP-2 to promote bone repair and regeneration. Keywords: cytokine delivery system, nanoparticles, chitosan-agarose-gelatin scaffold, stromal cell-derived factor-1, bone morphogenetic protein-2
语种：
英文
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核因子κB通过Sortilin促进荷脂THP-1巨噬细胞泡沫化
作者：
高安博;彭田红;钟丽园;孙莎;陈熙;...
期刊：
中国动脉硬化杂志 ,2018年26(7):666-671 ISSN：1007-3949
作者机构：
南华大学医学院应用解剖与生殖医学研究所,湖南省衡阳市,421001;[李素云; 万炜; 吕运成; 彭田红; 高安博; 谢巍; 钟丽园; 陈熙; 孙莎] 南华大学
关键词：
核因子κB;巨噬细胞;脂质蓄积;泡沫化
摘要：
目的探究核因子κB（NF-κB）是否通过分拣蛋白Sortilin影响荷脂THP-1巨噬细胞脂质代谢和泡沫化。方法 NF-κB激活剂PMA或其特异性抑制剂PDTC孵育荷脂THP-1巨噬细胞,qRT-PCR检测Sortilin mRNA水平,Western blot检测Sortilin蛋白表达;沉默荷脂THP-1巨噬细胞Sortilin同时加入PMA孵育,胞内胆固醇流出行液体闪烁计数器检测,胞内脂质含量行高效液相色谱法检测,胞内脂滴情况用油红O染色显示。结果 PMA孵育处理荷脂THP-1巨噬细胞后Sortilin mRNA水平及蛋白表达上调,PDTC孵育处理后Sortilin mRNA水平及蛋白表达下调;PMA孵育处理荷脂THP-1巨噬细胞内脂质流出减少,脂质蓄积程度增加,泡沫细胞形成加剧,而PMA与Sortilin shRNA共处理后,其胞内脂质流出增多,胞内脂质蓄积程度减轻,泡沫细胞形成受抑。结论 NF-κB通过促进Sortilin表达,导致巨噬细胞内脂质流出受抑,胞内脂质蓄积程度加重,从而加剧泡沫细胞形成。
语种：
中文
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丰富环境对辐射诱导小鼠认知障碍的保护作用及可能机制
作者：
乃爱桃;王贞;曹文宇;徐杨;刘政海;...
期刊：
中华放射医学与防护杂志 ,2018年38(6):401-406 ISSN：0254-5098
作者机构：
南华大学附属第一医院肿瘤放疗科, 衡阳, 421001;南华大学医学院应用解剖与生殖医学研究所, 衡阳, 421001;南华大学医学院生理教研室, 衡阳, 421001;南华大学医学院病理教研室, 衡阳, 421001;南华大学公共卫生学院, 衡阳, 421001
关键词：
电离辐射;认知功能障碍;丰富环境;小胶质细胞激活;突触
摘要：
目的研究丰富环境对辐射诱导小鼠认知功能障碍的保护作用及其可能机制。方法将45只2月龄雌性昆明小鼠采用随机数表法分为对照组、照射组和照射丰富环境组,每组15只。照射组和照射丰富环境组予以单次4 Gy全身~(137)Cs γ射线照射,照射丰富环境组辐射后连续35 d给予丰富环境刺激。新旧事物识别实验检测小鼠认知功能;免疫组织化学方法检测小鼠海马区小胶质细胞标记物IBA-1的表达;Western blot方法检测小胶质细胞激活标记物CD68及突触囊泡素(SYP)的表达。结果与对照组相比,照射组小鼠在新旧事物识别实验中新事物分辨率降低,海马区IBA-1阳性细胞数目增加,CD68蛋白表达升高,SYP蛋白表达降低(t=3.66、 6.83、 5.79、 6.84,P<0.05)。与照射组相比,照射丰富环境组小鼠新事物分辨率升高,海马区IBA-1阳性细胞数目减少,CD68蛋白表达降低,SYP蛋白表达增加(t=3.56、 7.69、 4.59、 4.06,P<0.05)。结论4 Gy单次全身~(137)Cs γ射线照射可构建放射性认知功能障碍模型,丰富环境可改善模型小鼠认知功能,其机制可能与抑制海马区小胶质细胞激活以及减少神经元突触丢失有关。
语种：
中文
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卓越医师临床技能教学改革的探讨
作者：
康颖;文格波;姜志胜;陈熙;唐志晗;...
期刊：
西部素质教育 ,2018年4(1):161-162 ISSN：2095-6401
作者机构：
南华大学医学院,湖南 衡阳,421001;南华大学附属第一医院,湖南 衡阳,421001;[唐志晗; 文格波; 康颖; 姜志胜; 陈熙] 南华大学;[刘红光] 南华大学第一附属医院
关键词：
卓越医师;临床技能;教学改革
摘要：
文章从卓越医师临床技能授课团队的优化及授课方式的改革;卓越医师人文素质的培养;提高医师英语沟通能力;提升科研文献阅读能力、加强科研思路培养四个方面探讨了卓越医师临床技能教学改革。
语种：
中文
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中期因子与动脉粥样硬化
作者：
阳镇;欧含笑;刘建;陈熙;莫中成
期刊：
生命的化学 ,2018年38(4):631-637 ISSN：1000-1336
作者机构：
南华大学应用解剖与生殖医学研究所,衡阳421001;南华大学医学院 衡阳421001;南华大学组织与胚胎学教研室,衡阳421001;南华大学解剖学教研室,衡阳421001;[阳镇; 刘建] 南华大学应用解剖与生殖医学研究所, 衡阳, 421001 南华大学医学院2016级临床医学专业, 衡阳, 421001
关键词：
动脉粥样硬化;中期因子;脂质代谢;炎症反应;氧化应激
摘要：
中期因子（midkine）是一种分泌型肝素结合生长因子,在炎症、肿瘤、代谢类疾病及胚胎的早期发育方面发挥着重要的作用。动脉粥样硬化（atherosclerosis）是一种常见的心血管疾病,迄今为止,其具体发病机制尚未完全阐明。研究表明,动脉粥样硬化是一种血管壁受损的慢性炎症性疾病,并与遗传、环境、脂质代谢相关。中期因子在脂质代谢、氧化应激及炎症等方面起重要作用,并与动脉粥样硬化密切相关。本文对中期因子在动脉粥样硬化发生发展中的作用的研究进展进行综述。
语种：
中文
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PBL教学模式在人体寄生虫学理论教学中的运用
作者：
王可耕;梁瑜;邹菊;李忠玉;陈熙;...
期刊：
山西医科大学学报 ,2018年20(3):165-167 ISSN：1007-6611
作者机构：
南华大学医学院寄生虫学教研室,衡阳,421001;南华大学医学院;[邹菊; 李忠玉; 刘彦; 王可耕; 陈熙; 梁瑜] 南华大学
关键词：
人体寄生虫学;教学模式;问题中心式学习法
摘要：
问题中心式学习法（problem-based learning,PBL）是现代医学教育中不同于传统教学模式的一种全新的教学模式,该教学模式以其独特的优越性在国外医学教育改革中取得了重要成效。文章介绍了PBL教学模式在人体寄生虫学理论教学中的运用,研究并探讨在人体寄生虫学教学中推行PBL教学方法的可能性。
语种：
中文
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“互联网+”背景下虚拟现实技术在卓越医生培养中的应用
作者：
李忠玉;刘彦;赵飞骏;向宇燕;陈熙;...
期刊：
山西医科大学学报 ,2018年20(9):799-801 ISSN：1007-6611
作者机构：
南华大学衡阳医学院病原生物研究所,衡阳,421001;南华大学衡阳医学院解剖学教研室;南华大学衡阳医学院病理生理学教研室;[李忠玉; 刘彦; 姜志胜; 向宇燕; 赵飞骏; 陈熙] 南华大学
关键词：
卓越医生教育;虚拟现实技术;互联网+
摘要：
“互联网＋”的便捷性、开放性和时效性的特性,以及虚拟现实技术交互性、沉浸性、构想性等优势,促进了两者与医学教育的深度融合。卓越医生教育是国家为培养高水平医学拔尖创新人才所实施的重大医学教育改革。文章分析了高校卓越医生培养教学存在的问题后,提出了加强虚拟仿真实验中心建设,完善“互联网＋”虚拟技术平台,加强师资队伍建设和优化互联网教育资源是提高卓越医生教育教学质量和效果的重要举措,为其他医学高等院校卓越医生培养计划的实施提供参考。
语种：
中文
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Axl inhibitors as novel cancer therapeutic agents
作者：
Shen, Yingying;Chen, Xiguang;He, Jun;Liao, Duanfang;Zu, Xuyu*
期刊：
Life Sciences ,2018年198:99-111 ISSN：0024-3205
通讯作者：
Zu, Xuyu
作者机构：
[Chen, Xiguang; Zu, Xuyu; Shen, Yingying] Univ South China, Affiliated Hosp 1, Inst Clin Med, 69 Chuanshan Rd, Hengyang 421001, Hunan, Peoples R China.;[He, Jun] Univ South China, Affiliated Nanhua Hosp, Dept Spine Surg, Hengyang 421001, Hunan, Peoples R China.;[Liao, Duanfang] Hunan Univ Chinese Med, Div Stem Cell Regulat & Applicat, Key Lab Qual Evaluat Bulk Herbs Hunan Prov, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Zu, Xuyu] U;Univ South China, Affiliated Hosp 1, Inst Clin Med, 69 Chuanshan Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
Axl inhibitors;Cancer;Receptor tyrosine kinase;Small chemicals;Targeted therapy
摘要：
Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers. Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Therefore, the research on Axl is promising and it is worthy of further investigations. In this review, we present an update on the Axl inhibitors and provide new insights into their latent application.
语种：
英文
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丰富环境对单眼剥夺小鼠视皮质与外侧膝状体Sortilin表达的影响
作者：
罗诗诗;高明;王昶仑;牛磊;罗丹;...
期刊：
神经解剖学杂志 ,2018年34(6):727-731 ISSN：1000-7547
作者机构：
南华大学医学院应用解剖与生殖医学研究所, 衡阳, 421001;南华大学医学院生理学教研室和神经科学研究所, 衡阳, 421001;[罗诗诗; 高明; 王昶仑; 牛磊; 罗丹; 李彩; 曹文宇; 万炜; 陈熙; 刘政海] 南华大学医学院应用解剖与生殖医学研究所, 衡阳, 421001;[徐杨] 南华大学医学院生理学教研室和神经科学研究所, 衡阳, 421001
关键词：
丰富环境;弱视;外侧膝状体;初级视皮质;小鼠
摘要：
目的:探讨丰富环境(EE)对弱视小鼠初级视皮质和外侧膝状体Sortilin的表达影响。方法:选择36只21 d昆明小鼠,雌雄不限,随机分为正常组(Control) 、单眼剥夺+标准环境组(MD + SE)和单眼剥夺+丰富环境组(MD + EE),通过行为学检测各组小鼠视功能改变、免疫组织化学方法检测各组小鼠视皮质和外侧膝状体Sortilin的表达。结果:行为学结果显示:与Control组相比,MD + SE组探爪触地成功率明显降低(P < 0.05),MD + EE组探爪触地成功率显著高于MD + SE组(P < 0.05);免疫组织化学结果显示:与Control组相比,MD + SE组外侧膝状体背内侧Sortilin阳性神经元数目显著升高(P < 0.001); MD + EE组显著低于MD + SE组,但高于Control组(P < 0.001);与Control组相比,MD + SE组小鼠视皮质Sortilin阳性神经元数目显著降低(P < 0.001); MD + EE组显著高于MD + SE组(P < 0.001),但显著低于Control组(P < 0.001) 。结论:丰富环境改善弱视小鼠视功能可能通过调节视皮质和外侧膝状体Sortilin的表达实现。
语种：
中文
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三七总皂苷对脂多糖诱导小鼠抑郁样行为及脑内小胶质细胞表达的影响
作者：
曾佳玉;王贞;牛磊;罗诗诗;刘政海;...
期刊：
解剖学报 ,2018年49(2):166-171 ISSN：0529-1356
作者机构：
南华大学医学院应用解剖学与生殖医学研究所, 湖南, 衡阳, 421001;南华大学医学院生理学教研室和神经科学研究所, 湖南, 衡阳, 421001;[曾佳玉; 王贞; 牛磊; 罗诗诗; 刘政海; 曹文宇; 万炜; 陈熙] 南华大学医学院应用解剖学与生殖医学研究所, 湖南, 衡阳, 421001;[徐杨] 南华大学医学院生理学教研室和神经科学研究所, 湖南, 衡阳, 421001
关键词：
三七总皂苷;脂多糖;抑郁;炎症;小胶质细胞;免疫组织化学;小鼠
摘要：
目的探讨三七总皂苷(PNS)对脂多糖(LPS)诱导的小鼠抑郁样行为及脑内小胶质细胞活化的影响。方法24只雄性昆明小鼠随机分为溶媒对照(NS)组、LPS组、三七总皂苷预处理(PNS + LPS)组,采用新奇环境抑制摄食实验、糖水偏好实验、悬尾实验、强迫游泳实验观察小鼠的抑郁样行为;采用免疫组织化学法检测海马、杏仁核小胶质细胞标记物离子钙接头蛋白分子1(IBA-1)的表达变化。结果LPS导致小鼠出现抑郁样行为,包括新奇环境摄食潜伏期延长(P < 0.05),糖水偏好百分比下降(P < 0.05),悬尾不动时间延长(P < 0.05),强迫游泳不动时间增加(P < 0.01),PNS干预可逆转上述抑郁行为表现(P < 0.05); LPS导致小鼠海马和杏仁核IBA-1阳性细胞数目显著增多(P < 0.0001),PNS预处理能明显减少IBA-1的阳性细胞数(P < 0.05) 。结论三七总皂苷能有效缓解LPS诱导的小鼠抑郁行为,其机制可能与抑制脑内小胶质细胞活化有关。
语种：
中文
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MiRNA-200a induce cell apoptosis in renal cell carcinoma by directly targeting SIRT1
作者：
Fu, Hao;Song, Wenke;Chen, Xuancai;Guo, Tao;Duan, Bin;...
期刊：
Molecular and Cellular Biochemistry ,2018年437(1-2):143-152 ISSN：0300-8177
通讯作者：
Huang, Liang;Zhang, Chi
作者机构：
[Duan, Bin; Chen, Xuancai; Wang, Xinxi; Fu, Hao; Tang, Yachun; Guo, Tao] Univ South China, Affiliated Nanhua Hosp, Dept Urol, Hengyang 421002, Hunan, Peoples R China.;[Song, Wenke] Univ South China, Affiliated Nanhua Hosp, Dept Obstet & Gynecol, Hengyang 421002, Hunan, Peoples R China.;[Huang, Liang] Univ South China, Sch Med, Dept Operat Surg, Hengyang 421002, Hunan, Peoples R China.;[Zhang, Chi] Univ South China, Sch Med, Funct Lab Ctr, Dept Pathophisiol, Hengyang 421002, Hunan, Peoples R China.
通讯机构：
[Huang, Liang; Zhang, Chi] U;Univ South China, Sch Med, Dept Operat Surg, Hengyang 421002, Hunan, Peoples R China.;Univ South China, Sch Med, Funct Lab Ctr, Dept Pathophisiol, Hengyang 421002, Hunan, Peoples R China.
关键词：
MiR-200a;SIRT1;Renal cell carcinoma;Apoptosis
摘要：
Accumulating evidence indicates that microRNAs are implicated in tumor initiation and progression through negatively regulating oncogenes or tumor suppressor genes. In the present study, we report that the expression of miR-200a was significantly lower in renal cell carcinoma (RCC) specimens and RCC cell lines. Restoration of miR-200a suppressed cell growth, arrested cell cycle progression, and promoted cell apoptosis in RCC cell lines. We next used qRT-PCR array technology to identify Sirtuin 1 (SIRT1) as one of the downregulated proteins during miR-200a overexpression in 786-O cells. Following a further assay by luciferase reporter system, SIRT1 was validated as a direct target of miR-200a. Moreover, siRNA-mediated knockdown of SIRT1 could partially phenocopy the effects of miR-200a overexpression. In contrast, overexpression of truncated SIRT1 (without an endogenous 3′-UTR) could rescue the effect of miR-200a overexpression on 786-O cells, which suggested that SIRT1 3′-UTR is targeted by miR-200a specifically. These observations provide further evidence for a critical tumor-suppressive role of the miR-200a in RCC in addition to identifying a novel regulatory mechanism, which may contribute to SIRT1 upregulation in RCC. © 2017, Springer Science+Business Media, LLC.
语种：
英文
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分拣受体Sortilin促进巨噬细胞内脂质蓄积
作者：
钟丽园;彭田红;高安博;万炜;陈熙;...
期刊：
中国动脉硬化杂志 ,2018年26(2):139-143 ISSN：1007-3949
作者机构：
南华大学医学院应用解剖与生殖医学研究所,湖南省衡阳市,421001;南华大学医学院心血管疾病研究所,湖南省衡阳市,421001;[王莉; 万炜; 唐志晗; 吕运成; 王佐; 彭田红; 高安博; 钟丽园; 陈熙; 欧阳宇甲] 南华大学
关键词：
巨噬细胞;脂质蓄积;氧化型低密度脂蛋白;动脉粥样硬化
摘要：
目的 探讨分拣受体Sortilin对THP-1巨噬细胞内脂质代谢的影响.方法 用氧化型低密度脂蛋白(ox-LDL)孵育THP-1巨噬细胞.Western blot检测荷脂后THP-1巨噬细胞中Sortilin表达变化;THP-1巨噬细胞Sortilin高表达和沉默后,液体闪烁计数仪检测胞内胆固醇流出,高效液相色谱法检测细胞内脂质含量,油红O染色观察胞内脂滴情况.结果 THP-1巨噬细胞Sortilin表达呈ox-LDL剂量和时间依赖性增加;以50 mg/L ox-LDL处理24 h后THP-1巨噬细胞Sortilin表达水平达到高峰.与对照组相比,Sortilin过表达后巨噬细胞胆固醇流出减少,胞内脂质含量增加且脂滴明显增多,而Sortilin沉默后则刚好出现相反的结果.结论 Sortilin抑制巨噬细胞内胆固醇流出,促进胞内脂质蓄积.
语种：
中文
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Sortilin promotes macrophage cholesterol accumulation and aortic atherosclerosis through lysosomal degradation of ATP-binding cassette transporter A1 protein
作者：
Lv, Yuncheng;Yang, Jing;Gao, Anbo;Sun, Sha;Zheng, Xilong;...
期刊：
生物化学与生物物理学报 ,2018年51(5):471-483 ISSN：1672-9145
通讯作者：
Peng, Tianhong;Zhong, Liyuan;Zhao, Guojun
作者机构：
[Chen, Xi; Sun, Sha; Lv, Yuncheng; Wan, Wei; Gao, Anbo; Xie, Wei; Peng, Tianhong; Guo, Dongming; Zhong, Liyuan; Li, Suyun; Peng, TH] Univ South China, Clin Anat & Reprod Med Applicat Inst, Hengyang 421001, Peoples R China.;[Yang, Jing] Univ South China, Affiliated Hosp 1, Clin Med Res Inst, Hengyang 421001, Peoples R China.;[Zheng, Xilong] Univ Calgary, Libin Cardiovasc Inst Alberta, Dept Biochem & Mol Biol, Hlth Sci Ctr, Calgary T2N 4N1, AB, Canada.;[Tang, Chaoke] Univ South China, Inst Cardiovasc Res, Med Res Ctr, Hengyang 421001, Peoples R China.;[Zhao, Guojun] Guilin Med Univ, Dept Histol & Embryol, Guilin 541004, Peoples R China.
通讯机构：
[Peng, TH; Zhong, LY] U;[Zhao, Guojun] G;Univ South China, Clin Anat & Reprod Med Applicat Inst, Hengyang 421001, Peoples R China.;Guilin Med Univ, Dept Histol & Embryol, Guilin 541004, Peoples R China.
关键词：
sortilin;ABCA1;macrophage;cholesterol efflux;atherosclerosis
摘要：
Sortilin is closely associated with hyperlipidemia and the risk of atherosclerosis (AS). The role of sortilin and the underlying mechanism in peripheral macrophage are not fully understood. In this study, we investigated the effect of macrophage sortilin on ATP-binding cassette transporter A1 (ABCA1) expression, ABCA1-mediated cholesterol efflux, and aortic AS. Macrophage sortilin expression was upregulated by oxidized low-density lipoproteins (ox-LDLs) in both concentrationand time-dependent manners. Its expression reached the peak level when cells were incubated with 50 ?g/ml ox-LDL for 24 h. Overexpression of sortilin in macrophage reduced cholesterol efflux, leading to an increase in intracellular total cholesterol, free cholesterol, and cholesterol ester. Sortilinwas found to bind with ABCA1 protein and suppress macrophage ABCA1 expression, resulting in a decrease in cholesterol efflux from macrophages. The inhibitory effect of sortilin in cholesterol efflux was partially reversed by treatment with chloroquine, a lysosomal inhibitor. On the contrary, the ABCA1 protein level and ABCA1-mediated cholesterol efflux is increased by sortilin short hairpin RNA transfection. The fecal and biliary cholesterol 3H-sterol from cholesterolladen mouse peritoneal macrophage was reduced by sortilin overexpression through lentivirus vector (LV)-sortilin in low-density lipoprotein receptor knockout mice, which was prevented by cotreatment with chloroquine. Treatment with LV-sortilin reduced plasma high-density lipoprotein and increased plasma ox-LDL levels. Accordingly, aortic lipid deposition and plaque area were exacerbated, and ABCA1 expression was reduced in mice in response to infection with LVsortilin alone. These effects of LV-sortilin were partially reversed by chloroquine. Sortilin enhances lysosomal degradation of ABCA1 protein and suppresses ABCA1-mediated cholesterol efflux from macrophages, leading to foam cell formation and AS development. © 2019 The Author(s) .
语种：
英文
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丰富环境改善小鼠放射性认知功能障碍
作者：
唐丽云;乃爱桃;王贞;徐杨;刘政海;...
期刊：
辐射研究与辐射工艺学报 ,2018年36(6):1-7 ISSN：1000-3436
作者机构：
南华大学附属第一医院肿瘤放疗科, 衡阳, 421001;南华大学医学院应用解剖与生殖医学研究所, 衡阳, 421001;南华大学医学院生理教研室, 衡阳, 421001;南华大学医学院病理教研室, 衡阳, 421001;南华大学公共卫生学院放射医学教研室, 衡阳, 421001
关键词：
丰富环境;海马区;环磷酸腺苷应答元件结合蛋白(CREB);认知功能障碍;神经发生;电离辐射
摘要：
探讨丰富环境(Environmental Enrichment, EE)对电离辐射所致小鼠认知功能障碍的保护作用及其可能机制。将36只雌性昆明小鼠随机分为对照组(Control)、辐射组(IR)和辐射丰富环境组(IR+EE)。IR组和IR+EE组小鼠采用137Cs γ射线进行全身辐照至吸收剂量4 Gy;IR+EE组小鼠辐照后给予连续35 d EE刺激。采用新物体识别实验检测小鼠认知功能;免疫组织化学方法检测海马区神经发生标记物双皮质素(Doublecortin, DCX)及磷酸化环磷酸腺苷应答元件结合蛋白(Phosphorylated cyclic adenosine monophosphate (cAMP) response element-binding protein, p-CREB)的表达;Western blot方法检测海马区CREB及p-CREB蛋白的表达。结果表明:与对照组相比,IR组小鼠新物体分辨率明显降低(45.55 ± 5.80 vs. 2.99 ± 6.18,p<0.05),海马区DCX阳性细胞数明显减少(123.8 ± 9.3 vs. 70.2 ± 5.9,p<0.05),p-CREB/CREB表达下调(1.007 ± 0.058 vs. 0.772 ± 0.039, p<0.05);而予以EE刺激后小鼠新物体分辨率明显回升(2.99 ± 6.18 vs. 28.31 ± 7.30,p<0.05),海马区DCX阳性细胞数明显增加(70.2 ± 5.9 vs. 95.7 ±6.5,p<0.05),p-CREB/CREB表达上调(0.772 ± 0.039 vs. 1.014 ± 0.093, p<0.05)。结果提示EE可能是通过上调海马区p-CREB的表达,保护海马神经发生,进而改善辐射所致的小鼠认知功能障碍。
语种：
中文
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基础与临床全方位相结合的护理专业系统解剖学教学模式探讨
作者：
王爱平;唐谟建;李严兵;陈胜华;彭田红;...
期刊：
山西医科大学学报 ,2017年19(3):173-175 ISSN：1007-6611
作者机构：
南华大学医学院解剖学教研室,衡阳,421001;[王爱平; 彭田红; 陈胜华; 谭建国; 李严兵; 陈熙; 唐谟建] 南华大学
关键词：
系统解剖学;基础与临床;教学改革;护理专业
摘要：
文章就护理专业基础与临床全方位相结合的系统解剖学教学模式进行论述,围绕护理专业系统解剖学教学为何要将基础与临床全方位相结合以及如何做到这一点作了详细探讨。通过精选教材内容、PBL教学模式、体表标志的触摸、实验教学学生自主观察并课后绘图以及课后反馈等方式可以使系统解剖学与临床护理全方位地结合在一起。相比传统护理专业系统解剖学教学模式,新的教学模式教学重点突出、学生参与积极性高涨、学生掌握知识结构更完整,对于护理专业学生学好系统解剖学并提高其自学能力、实践能力和创新能力具有重要作用。
语种：
中文
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医学人文教育在解剖学教学中的应用
作者：
徐皓南;王爱平;安高;彭田红;徐菁;...
期刊：
教育现代化 ,2017年(11):102-103 ISSN：2095-8420
作者机构：
南华大学 医学院解剖学教研室,湖南 衡阳,421001;[王爱平; 徐菁; 彭田红; 徐皓南; 陈熙; 安高] 南华大学
关键词：
医学人文教育;解剖学教学;应用
摘要：
解剖学是医学重要的基础学科,是涉及生命体的结构和组织的生物学分支学.医学人文教育与解剖学教学相结合,将会有力地变革现有的解剖学教学氛围,创造崭新的教学体系,呼应教改大潮,提高医学生的人文素养,为将来的国家培养一批有能力,更有情感的合格医生.
语种：
中文
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Implications of sortilin in lipid metabolism and lipid disorder diseases
作者：
Gao, Anbo;Cayabyab, Francisco S.;Chen, Xi;Yang, Jing;Wang, Li;...
期刊：
DNA AND CELL BIOLOGY ,2017年36(12):1050-1061 ISSN：1044-5498
通讯作者：
Peng, Tianhong;Lv, Yuncheng
作者机构：
[Chen, Xi; Wang, Li; Gao, Anbo; Peng, Tianhong; Lv, Yuncheng; Peng, TH; Lv, YC] Univ South China, Clin Anat & Reprod Med Applicat Inst, Hengyang 421001, Peoples R China.;[Cayabyab, Francisco S.; Lv, Yuncheng] Univ Saskatchewan, Dept Surg, Coll Med, Saskatoon, SK, Canada.;[Yang, Jing] Univ South China, Affiliated Hosp 1, Dept Endocrinol & Metab, Hengyang, Peoples R China.
通讯机构：
[Peng, TH; Lv, YC] U;Univ South China, Clin Anat & Reprod Med Applicat Inst, Hengyang 421001, Peoples R China.
关键词：
atherosclerosis;lipid metabolism;nonalcoholic fatty liver disease;sortilin;type 2 diabetes mellitus
摘要：
The present review provides a summary of recent evidence of sortilin expression, function, and regulation and its implications in lipid metabolism and development of lipid disorder diseases. As a member of the vacuolar protein sorting 10 protein (Vps10p) receptor family, sortilin mediates intracellular trafficking of diverse endogenous or exogenous protein substrates between the trans-Golgi network (TGN) and plasma membrane compartments. Recent studies reveal that sortilin regulates the expression of lipid genes, plasma lipid level, and the development of lipid disorder diseases. Sortilin promotes atherogenesis by regulating hepatic very low density lipoprotein (VLDL) secretion and plasma lipid level and subsequently macrophage lipid accumulation. Sortilin deficiency is caused by accelerated proteasome degradation under insulin resistance conditions and is thereby implicated in the hyperlipidemia of type 2 diabetes mellitus (T2DM). Sortilin facilitates hepatic cholesterol accumulation by inhibiting hepatic cholesterol catabolism, which promotes the development of nonalcoholic fatty liver disease (NAFLD). Sortilin plays an important role in lipid metabolism and represents a promising therapeutic target for lipid disorder diseases.
语种：
英文
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Morphometric measurement of the cervical spine for minimally invasive pedicle screw fixation using reverse engineering and three-dimensional reconstruction
作者：
Zhou, Zi-Jia;Wen, Chao-Lun;Sun, Qin-Min;Wang, Ai-Ping;Yan, Zheng-Guang;...
期刊：
INTERNATIONAL JOURNAL OF MEDICAL ROBOTICS AND COMPUTER ASSISTED SURGERY ,2017年13(3):e1765- ISSN：1478-5951
通讯作者：
Li, Yan-Bing
作者机构：
[Chen, Xi; Wang, Ai-Ping; Li, Yan-Bing; Zhou, Zi-Jia; Sun, Qin-Min; Zhou, Xiao-Bing; Tan, Jian-Guo; Liu, Fang] Univ South China, Dept Anat, 28 W Changsheng Rd, Hengyang City 421001, Hunan, Peoples R China.;[Wen, Chao-Lun] PLA, Hosp 169, Spinal Surg Dept, Hengyang City 421001, Hunan, Peoples R China.;[Yan, Zheng-Guang] PLA, Hosp 169, Dept Radiol, Hengyang City 421001, Hunan, Peoples R China.;[Cao, Qi] Univ South China, Affiliated Hosp 2, Spinal Surg Dept, Hengyang City 421001, Hunan, Peoples R China.
通讯机构：
[Li, Yan-Bing] U;Univ South China, Dept Anat, 28 W Changsheng Rd, Hengyang City 421001, Hunan, Peoples R China.
关键词：
spinous process;cervical spine;pedicle;computed tomography reconstruction;reverse engineering
摘要：
Background: Percutaneous cervical pedicle screw fixation has been proven to be an effective method of cervical screw instrumentation, which has the advantages of less invasiveness and low blood loss. Emerging evidence has indicated that the cervical spinous process plays an important role in percutaneous spine surgery. However, there is a limited amount of information on the fundamental research of pedicle and its associated imaging parameter measurement. The purpose of this study was to measure the anatomic data of the pedicle screw channel (PSC) using reverse engineering and three-dimensional reconstruction, and also to discuss the three-dimensional relationship between the cervical spinous process and the pedicle screw channel. Methods: Twenty adult subjects (10 males, 10 females, age range 19–46 years) were studied using the method of three-dimensional CT reconstruction and reverse engineering. The centrum was divided into 10 equal parts from front to back. The bisectors were defined as borderline depths of the centrum, from front to back, 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10%, 0% of borderline depths were presented. Then, a 3D coordinate system was constructed to measure all the data, including the radius of the inscribed circle, the length of the PSC, the insertion angle, the distances from entry point to cervical spinous process and skin depth. All the indexes were measured from 70% to 90% borderline depth. Results: The radius of the inscribed circles from C3 to C7 at 90% borderline depth were 2.94 ± 0.55 mm, 3.04 ± 0.40 mm, 3.15 ± 0.36 mm, 3.28 ± 0.47 mm, 3.89 ± 0.54 mm, respectively. The lengths of the PSC were between 25 and 32 mm. The insertion angles for 70% to 90% borderline depth were 28.33°, 34.28°, 37.92°, respectively. The relationship between the PSC and spinous process was measured as the distance from the entry point to the end of the spinous process, which were, respectively, 26.91 mm, 28.18 mm, 30.03 mm, 35.67 mm, 41.99 mm from C3 to C7.The distance from the skin to the entry point of C3-7 increased gradually. Conclusions: The measurements of this study could provide detailed information for percutaneous cervical screw fixation. The data of the relationship between the cervical spinous process and the pedicle screw channel present valuable technical information for the design, optimization and clinical application of the aiming device for percutaneous cervical pedicle screw fixation. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.
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英文
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Identification of novel mutations in the HbF repressor gene BCL11A in patients with autism and intelligence disabilities
作者：
Cai, Tao* ;Chen, Xiang;Li, Jinchen;Xiang, Bingwu;Yang, Liu;...
期刊：
American Journal of Hematology ,2017年92(12):E653-E656 ISSN：0361-8609
通讯作者：
Cai, Tao;Liu, P. Paul
作者机构：
[Cai, Tao; Li, Jinchen] Wenzhou Med Univ, Inst Genom Med, Wenzhou, Zhejiang, Peoples R China.;[Cai, Tao] Natl Inst Dent & Craniofacial Res, NIH, Bethesda, MD 20892 USA.;[Liu, Yidian; Xiang, Bingwu; Yang, Liu; Chen, Xiang] Wenzhou Med Univ, Phys Med & Rehabil Ctr, Affiliated Hosp 2, Wenzhou, Zhejiang, Peoples R China.;[Liu, Yidian; Xiang, Bingwu; Yang, Liu; Chen, Xiang] Yuying Childrens Hosp, Wenzhou, Zhejiang, Peoples R China.;[Chen, Qiuli] Maternal & Child Hlth Hosp Guangxi Zhuang Autonom, Genet Metab CentraLand Lab, Nanning, Guangxi, Peoples R China.
通讯机构：
[Cai, Tao; Liu, P. Paul] N;Natl Inst Dent & Craniofacial Res, NIH, Bethesda, MD 20892 USA.;NHGRI, NIH, Bethesda, MD 20892 USA.
语种：
英文
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8-Bromo-7-methoxychrysin-blocked STAT3/ Twist axis inhibits the stemness of cancer stem cell-like cell originated from SMMC-7721 cells
作者：
Luo, Yimin;Cui, Yinghong;Cao, Xiaocheng;Li, Xiang;Chen, A.;...
期刊：
生物化学与生物物理学报 ,2017年49(5):458-464 ISSN：1672-9145
通讯作者：
Cao, Jianguo
作者机构：
[Luo, Yimin] Univ South China, Coll Med, Inst Canc, Hengyang 421001, Peoples R China.;[Luo, Yimin] Univ South China, Key Lab Tumor Cellular & Mol Pathol, Hengyang 421001, Peoples R China.;[Luo, Yimin] Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.;[Zhang, Jiansong; Cui, Yinghong; Li, Xiang; Cao, Jianguo; Chen, A.] Hunan Normal Univ, Coll Med, Dept Pharmaceut Sci, Changsha 410013, Hunan, Peoples R China.;[Chen, Xiangding; Cao, Xiaocheng] Hunan Normal Univ, Lab Mol & Stat Genet, Changsha 410081, Hunan, Peoples R China.
通讯机构：
[Cao, Jianguo] H;Hunan Normal Univ, Coll Med, Dept Pharmaceut Sci, Changsha 410013, Hunan, Peoples R China.
关键词：
liver cell carcinoma;cancer stem cell;8-bromo-7-methoxychysin;STAT3;Twist
摘要：
Signal transducer and activator of transcription 3 (STAT3) is a member of the family of latent cytoplasmic transcriptional factors that could regulate cell proliferation, survival, and development. It has been reported that Twist is a target gene of STAT3, and STAT3/Twist signaling plays an important role in regulating cancer progress. Here, to explore whether 8-bromo-7-methoxychrysin (BrMC) inhibits liver cancer stem-like cell (LCSLC) properties via disrupting STAT3/Twist signaling, we cultured SMMC-7721 cells in vitro, and evaluated the effects of BrMC on the stemness of spheroids by determining the sphere-forming capability and migration. The sphere formation assay results showed a concentration-dependent decrease of sphere-forming capacity in LCSLCs (P < 0.05) treated with different concentrations of BrMC. Wound-healing assays results demonstrated a concentration-dependent decline in cell migration of LCSLCs treated with different concentrations of BrMC. In addition, CD133, CD44, and ALDH1 levels were decreased in LCSLCs treated with BrMC. Treatment with different concentrations of BrMC also reduced the expressions of p-STAT3 and Twist1 proteins. The effect of BrMC was substantially enhanced by co-treatment with JSI-124, a specific inhibitor of STAT3. Ectopic expression of Twist1 attenuated the inhibitory effects of BrMC on sphere formation, migration, and expression of the markers in LCSLCs. However, it had no affect on p-STAT3 expression in LCSLCs. These results demonstrated that BrMC inhibits the stemness of LCSLCs originated from SMMC-7721 cell line by inhibiting STAT3/ Twist signal axis.
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英文
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