作者机构：
[Qin, Xinru; Liu, Xiaocheng; Li, Guojuan; Tang, Yonghong; Rang, Ouyan; Wang, Mu; Cao, Lin] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Clin Mass Spectrometry Lab,Clin Res Inst, Hengyang 421001, Hunan, Peoples R China.;[Qin, Xinru; Liu, Xiaocheng; Li, Guojuan; Tang, Yonghong; Rang, Ouyan; Wang, Mu; Cao, Lin] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Basic Med,Nucl Ind Hyg Sch, Hengyang 421001, Hunan, Peoples R China.;[Qin, Xinru] Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Zhong, Jing; Zhong, J] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Canc Res Inst,Inst Clin Med, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Zhong, J ; Wang, M ] U;Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Clin Mass Spectrometry Lab,Clin Res Inst, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Basic Med,Nucl Ind Hyg Sch, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Canc Res Inst,Inst Clin Med, Hengyang 421001, Hunan, Peoples R China.
摘要：
The consumption of fructose has increased dramaticly during the last few decades, inducing a great increase in the risk of intrahepatic lipid accumulation, hypertriglyceridemia, hyperuricemia and cancer. However, the underlying mechanism has not yet been fully elucidated. Amino acid metabolism may play an important role in the process of the diseases caused by fructose, but there is still a lack of corresponding evidence. In present study, we provide an evidence of how fructose affects amino acids metabolism in 1895 ordinary residents in Chinese community using UPLC-QqQMS based amino acid targeted metabolomics and the underlying mechanism of fructose exposure how interferes with amino acid metabolism related genes and acetylated modification of proteome in the liver of rats model. We found people with high fructose exposure had higher levels of Asa, EtN, Asp, and Glu, and lower levels of 1MHis, PEtN, Arg, Gln, GABA, Aad, Hyl and Cys. The further mechanism study displayed amino acid metabolic genes of Aspa, Cndp1, Dbt, Dmgdh, and toxic metabolites such as N-acetylethanolamines accumulation, interference of urea cycle, as well as acetylated modification of key enzymes in glutamine metabolic network and glutamine derived NEAAs synthesis pathway in liver may play important roles in fructose caused reprogramming in amino acid metabolism. This research provides novel insights of the mechanism of amino acid metabolic disorder caused by fructose and supplies new targets for clinical therapy.
摘要：
Holmium (Ho) modified TiO2 (Ho-TiO2) nanocomposites have been prepared by a sol-gel process, and Copyright: American Scientific Publishers were used as an effective adsorbent and matrix for the erichment and analysis of bisphenol S and Indigo Delivered by Ingenta by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The low detection limits of bisphenol S (BPS) and Indigo (ID) are 0.1 pg center dot mL-1 and 5 pg center dot mL-1, respectively. Extremely BPS detection could be achieved in tea samples with good recovery (81.5-93.0%).
通讯机构：
[Tao, L.; Luo, C.] D;[Wang, F.] T;Department of Forensic Medicine, School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, 215123, China
摘要：
Although traumatic brain injury (TBI) is a common cause of death and disability worldwide, there is currently a lack of effective therapeutic drugs and targets. To reveal the complex pathophysiologic mechanisms of TBI, we performed transcriptome analysis of the mouse cerebral cortex and immunohistochemical analysis of human cerebral tissues. The genes Mt1, Mt2, Il33, and Fth1 were upregulated post-TBI and enriched in pathways associated with the inflammatory response, oxidative phosphorylation, and ferroptosis. As an agonist of MT1/2, melatonin (MLT) confers anti-oxidant, anti-inflammatory, and anti-ferroptosis effects after TBI. However, whether these upregulated genes and their corresponding pathways are involved in the neuroprotective effect of MLT remains unclear. In this study, interventions to inhibit MT1/2, IL-33, and ferroptosis (i.e., ferritin H (Fth)-KO) were applied post-TBI. The results showed that MLT attenuated TBI-induced cerebral edema and neurological outcomes by inhibiting inflammation and ferroptosis. Mechanistically, MLT mainly suppressed inflammatory responses and ferroptosis via the activation of MT2 and IL-33 pathways. Building on the previous finding that Fth deletion increases susceptibility to ferroptosis post-TBI, we demonstrated that Fth depletion remarkably exacerbated the post-TBI inflammatory response, and abolished the anti-inflammatory effects of MLT both in vivo and in vitro. Furthermore, the post-TBI anti-inflammatory effect of MLT, which occurs by promoting the polarization of CD206(+) macrophages, was dependent on Fth. Taken together, these results clarified that MLT alleviates inflammation- and ferroptosis-mediated brain edema and neurological deficits by activating the MT2/IL-33/Fth pathway, which provides a novel target and theoretical basis for MLT to treat TBI patients.
摘要：
The main types of functional nucleic acids, signal amplification elements, and signal amplification technologies for detecting foodborne hazardous substances are described. Chen and coworkers have summarized the advantages and disadvantages of current detection methods based on functional nuclear acids biosensors, and put forward some suggestions for developing more effective detection methods of foodborne hazardous substances, which provide some significant references for the efficient detection of foodborne hazardous substances based on functional nucleic acids in the future. Abstract With the further improvement of food safety requirements, the development of fast, highly sensitive, and portable methods for the determination of foodborne hazardous substances has become a new trend in the food industry. In recent years, biosensors and platforms based on functional nucleic acids, along with a range of signal amplification devices and methods, have been established to enable rapid and sensitive determination of specific substances in samples, opening up a new avenue of analysis and detection. In this paper, functional nucleic acid types including aptamers, deoxyribozymes, and G‐quadruplexes which are commonly used in the detection of food source pollutants are introduced. Signal amplification elements include quantum dots, noble metal nanoparticles, magnetic nanoparticles, DNA walkers, and DNA logic gates. Signal amplification technologies including nucleic acid isothermal amplification, hybridization chain reaction, catalytic hairpin assembly, biological barcodes, and microfluidic system are combined with functional nucleic acids sensors and applied to the detection of many foodborne hazardous substances, such as foodborne pathogens, mycotoxins, residual antibiotics, residual pesticides, industrial pollutants, heavy metals, and allergens. Finally, the potential opportunities and broad prospects of functional nucleic acids biosensors in the field of food analysis are discussed.
作者机构：
[Yang, Shengyuan; Tan, Yan; Su, Wenen; Liu, Jinquan; Fan, Pengfei; Li, Qianji] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.;[Wu, Xiwen] Yueyang Cty Ctr Dis Control & Prevent, Yueyang 414100, Hunan, Peoples R China.;[Zhang, Xiaohuan] Yongding Dist Ctr Dis Control & Prevent, Zhangjiajie 427000, Hunan, Peoples R China.
通讯机构：
[Li, Qianji] D;Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, People's Republic of China.
关键词：
"On-off";Self-powered biosensor;Enzymatic biofuel cells;Capture and release
摘要：
A "on-off" self-powered biosensor was developed based on enzymatic biofuel cells (EBFCs) for sequential detection of microRNA-21 (miRNA-21) and miRNA-155 by capture and release of a single-bioanode enzyme, i.e., glucose oxidase. When miRNA-21 is present, it hybridizes with DNA1 and the glucose oxidase (GOD)-modified DNA2, i.e., DNA2-GOD. Then, GOD oxidizes glucose to produce a large number of electrons, and a significantly increased open-circuit voltage (E1OCV) is observed, corresponding to the "on" state. If miRNA-155 is present, it hybridizes with DNA1 and DNA3-functionalized SiO2 nanosphere@gold nanoparticles, i.e., SiO2@AuNPs-DNA3, and replaces miRNA-21 to release GOD at the bioanode, thus leading to decreased E2OCV, corresponding to the "off" state. The "on-off" self-powered biosensor shows ultra-sensitive detection of miRNA-21 and miRNA-155 with detection limits of 0.17 fM and 0.37 fM, respectively. It is believed that this study provides a feasible model for designing self-powered biosensors for multi-targets detection.
作者：
Cao, Wandi;Liu, Mingkang;Wang, Canyang;Jing, Guoxing;Cao, Yi
期刊：
Journal of Applied Toxicology,2023年43(5):706-718 ISSN：0260-437X
通讯作者：
Guoxing Jing<&wdkj&>Yi Cao
作者机构：
[Liu, Mingkang; Jing, Guoxing; Cao, Wandi] Xiangtan Univ, Sch Chem Engn, Xiangtan, Peoples R China.;[Wang, Canyang; Cao, Yi] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang, Peoples R China.;[Jing, Guoxing] Xiangtan Univ, Sch Chem Engn, Xiangtan 511105, Peoples R China.;[Cao, Yi] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.
通讯机构：
[Guoxing Jing] S;[Yi Cao] H;School of Chemical Engineering, Xiangtan University, Xiangtan, China<&wdkj&>Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China
关键词：
3D Caco-2 spheroids;Kruppel-like factors (KLFs);mouse intestines;RNA-sequencing;TiO2 nanoparticles (NPs)
摘要：
Kruppel-like factors (KLFs) are a set of transcription factors (TFs) involved in the regulation of many basic biological processes, and recent studies suggested that nanoparticles (NPs) were capable to change KLFs in different models even at non-cytotoxic concentrations. In this study, we repeatedly exposed 3D Caco-2 spheroids and mice to TiO(2) NPs, one of the most frequently used metal oxide NPs, and investigated the changes of KLF-signaling pathways based on RNA-sequencing. Although the internalization of TiO(2) NPs did not induce cytotoxicity in vitro, repeated exposure (three times within 7 days) to 15.7 ng/ml TiO(2) NPs increased KLF4 but decreased KLF6. Consistently, KLF4/KLF6-regulated gene ontology terms were altered, including those involved in the regulation of gene expression. We further verified that repeated exposure to 15.7 ng/ml TiO(2) NPs increased the expression of KLF4 and proto-oncogene, bHLH transcription factor (MYC), but decreased the expression of KLF6 and activating transcription factor 3 (ATF3). But with the increase of NP concentrations, the expression of these genes was decreased. In mice following intragastrical exposure to 4.39 and 43.9 mg/kg TiO(2) NPs (once a day for 5 continuous days), we observed increased expression of klf4, klf6, myc, and atf3, along with morphological changes of intestines. We concluded that repeated exposure to low levels of TiO(2) NPs altered KLF-signaling pathways in intestinal cells both in vitro and in vivo.
作者机构：
[Yang, Fei; Veerabadhran, Maruthanayagam] Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang, Peoples R China.;[Veerabadhran, Maruthanayagam] Cent South Univ, Xiangya Sch Publ Hlth, Hunan Prov Key Lab Clin Epidemiol, Hunan 410078, Peoples R China.;[Manivel, Nagarajan] ICAR Cent Marine Fisheries Res Inst, Chennai 6000028, India.;[Sarvalingam, Barathkumar] Minist Earth Sci, Natl Ctr Coastal Res NCCR, NIOT Campus, Chennai 600100, India.;[Seenivasan, Boopathi] SRM Inst Sci & Technol, Coll Sci & Humanities, Dept Biotechnol, Chennai, India.
通讯机构：
[MubarakAli Davoodbasha] S;[Fei Yang] H;School of Life Sciences, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai 600 0048, India<&wdkj&>Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, University of South China, Hengyang, China
摘要：
目的了解2021年衡阳市生畜肉及熟肉制品中沙门菌污染状况,分析沙门菌血清型分布、分子分型特征及耐药情况。方法采集衡阳市各县区生畜肉及熟肉制品样品,依照GB4789.4进行沙门菌分离鉴定和血清分型;采用美国临床和实验室标准...展开更多 目的了解2021年衡阳市生畜肉及熟肉制品中沙门菌污染状况,分析沙门菌血清型分布、分子分型特征及耐药情况。方法采集衡阳市各县区生畜肉及熟肉制品样品,依照GB4789.4进行沙门菌分离鉴定和血清分型;采用美国临床和实验室标准化协会(Clinical and Laboratory Standards Institute,CLSI)推荐的微量肉汤稀释法进行药物敏感试验;用脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)进行分子分型,结果用BioNumerics软件聚类分析。结果从52份生畜肉中检出18株沙门菌,检出率为34.62%(18/52),52份熟肉制品中检出3株沙门菌,检出率为5.77%(3/52);21株沙门菌可分为7个血清型,其中伦敦沙门菌42.86%(9/21)和罗森沙门菌19.05%(4/21)占主要优势;21株沙门菌均对亚胺培南(IPM)和多粘菌素E(CT)敏感,对萘啶酸(NAL)、头孢噻肟(CTX)、头孢西丁(CFX)、头孢他啶(CAZ)敏感度均达90.00%以上,对四环素(TET)、氨苄西林(AMP)、甲氧苄啶/磺胺甲噁唑(SXT)耐药达50.00%以上,多重耐药严重,最常见的耐药谱为四环素-氨苄西林-甲氧苄啶/磺胺甲噁唑(TET-AMP-SXT),耐药率为52.38%;21株沙门菌PFGE聚类分析相似度为48.20%,罗森、伦敦沙门菌带型最为集中,但鼠伤寒沙门菌呈现多样性;各带型与耐药谱之间未见明显相关。结论衡阳市生畜肉及熟肉制品沙门菌污染及耐药问题严重,血清型分布广泛,同一血清型大部分菌株PFGE型可聚集成簇。收起
