摘要：
Polysaccharide chitosan and L-histidine were applied to synthesize chitosan-based carbon dots (CA-CDs) by a simple laser ablation method. After characterization of the CA-CDs by FT-IR, UV-vis, Raman, XRD, TEM, and XPS, the CA-CDs were introduced as an eco-friendly and high-performance corrosion inhibitor for mild steel (MS) in 1.0M HCl solution. The inhibition action and mechanism of CA-CDs were determined by weight loss and electrochemical measurements, in combination with SEM, AFM, and XPS. The results show that CA-CDs as mixed-type inhibitors could effectively weaken the corrosion of MS in 1.0M HCl solution, and their maximum inhibition efficiency reaches 97.4% at 40mgL(-1). The adsorption behavior of CA-CDs well obeys the Langmuir adsorption isotherm containing both chemisorption and physisorption. The chemisorption mainly results from the multiple adsorption sites in the CA-CDs, and the physical adsorption is due to the blocking and barrier effect of CA-CD nanoparticles. Both adsorption behaviors were proposed to elucidate the corrosion inhibition mechanism of CA-CDs.
作者机构：
[童学智; 杨胜园] Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China;[陈东洋; 范翔; 张昊; 冯家力] Hunan Provincial Center for Disease Control and Prevention, Changsha 410005, China
通讯机构：
[Dongyang, C.] H;Hunan Provincial Center for Disease Control and PreventionChina
作者机构：
[Hu, Fangyan; Long, Dingxin; Hu, Zehui; Ou, Yiquan; Shi, Shengyuan] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Long, Dingxin] Univ South China, Sch Publ Hlth, Hengyang, Peoples R China.
通讯机构：
[Long, Dingxin] H;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, PR China. Electronic address:
摘要：
Mitochondria-associated endoplasmic reticulum membranes (MAMs) are dynamic suborganelle membranes that physically couple endoplasmic reticulum (ER) and mitochondria to provide a platform for exchange of intracellular molecules and crosstalk between the two organelles. Dysfunctions of mitochondria and ER and imbalance of intracellular homeostasis have been discovered in the research of toxics. Cellular activities such as oxidative stress, ER stress, Ca2+ transport, autophagy, mitochondrial fusion and fission, and apoptosis mediated by MAMs are closely related to the toxicological effects of various toxicants. These cellular activities mediated by MAMs crosstalk with each other. Regulating the structure and function of MAMs can alleviate the damage caused by toxicants to some extent. In this review, we discuss the relationships between MAMs and the mechanisms of toxicological effects, and highlight MAMs as a potential target for protection against toxicants.
期刊：
Bioinorganic Chemistry and Applications,2023年2023 ISSN：1565-3633
通讯作者：
Li, R.;Feng, W.
作者机构：
[Song, Jingfang; Feng, Wei] Univ South China, Affiliated Hosp 2, Inst Pathogen Biol, Hengyang Med Sch,Dept Pediat, Hengyang 421001, Hunan, Peoples R China.;[Song, Jingfang; Feng, Wei; Li, Ranhui] Hunan Prov Key Lab Special Pathogens Prevent & Con, Hengyang 421001, Hunan, Peoples R China.;[Wang, Weiguo; Yang, Lin; He, Jian] Univ South China, Inst Pharm & Pharmacol, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Bai, Qinqin] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.;[Li, Ranhui] Univ South China, Inst Pathogen Biol, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Qinqin Bai] D;[Wei Feng; Jingfang Song] T;[Jian He; Ranhui Li; Weiguo Wang; Lin Yang] I;Institute of Pharmacy and Pharmacology,Hengyang Medical School,Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study,China<&wdkj&>Institute of Pathogenic Biology,Hengyang Medical School,University of South China and Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control,China<&wdkj&>College of Basic Medical Sciences,Youjiang Medical University for Nationalities,Baise Guangxi 533000,China<&wdkj&>Department of Public Health Laboratory Science,School of Public Health,Hengyang Medical School,China<&wdkj&>The Second Affiliated Hospital,Department of Pediatrics,Institute of Pathogenic Biology,China
关键词：
Introduction;Materials and Methods;Results;Discussion;Conclusion;Abstract;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interests;Authors’ Contributions;Funding Statement;Acknowledgements;Acknowledgments;Supplementary Materials;Reference;Dataset Description;Dataset Files;Abstract;Introduction;Introduction and Materials;Introduction and Methods;Materials;Materials and Methods;Methods;Results;Discussion;Results and Discussion;Discussion and Conclusion;Results and Conclusion;Conclusion;Conclusions;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interest;Authors’ Contributions;Funding Statement;Acknowledgements;Supplementary Materials;References;Appendix;Abbreviations;Preliminaries;Introduction and Preliminaries;Notation;Proof of Theorem;Proofs;Analysis of Results;Examples;Numerical Example;Applications;Numerical Simulation;Model;Model Formulation;Systematic Palaeontology;Nomenclatural Acts;Taxonomic Implications;Experimental;Synthesis;Overview;Characterization;Background;Experimental;Theories;Calculations;Model Verification;Model Implementation;Geographic location;Study Area;Geological setting;Data Collection;Field Testing;Data and Sampling;Dataset;Literature Review;Related Works;Related Work;System Model;Methods and Data;Experimental Results;Results and Analysis;Evaluation;Implementation;Case Presentation;Case Report;Search Terms;Case Description;Case Series;Background;Limitations;Additional Points;Case;Case 1;Case 2 etc.;Concern Details;Retraction Details;Copyright;Related Articles
摘要：
The widespread use of antibiotics results in harmful residues, posing a serious threat to ecosystems and human health. Given synergetic or antagonistic toxicological features often exist among different antibiotics, it is vital to develop methods to synchronously determine these contaminants. Herein, we develop a hand-held photo-luminescent sensor array based on "turn-off" bimetallic lanthanide metal-organic frameworks (MOFs) to realize the identification and quantification of six common antibiotics. Three Tb/Eu-MOFs with different bimetallic proportions were synthesized and employed to provide signals toward multiple antibiotics based on their dif-ferential contributions to the photoluminescence quenching of these probes. By designing a 3D-printed smartphone-equipped signal capturing platform, tiny luminescence variations induced by different antibiotics were recorded and processed for the classification and identification of these targets via pattern recognition analysis. Compared to conventional sensor arrays requiring cumbersome signal reading instruments and complex sensing units, our platform really enables instrument-free in-field detection of multiple targets with only a basic material. The platform could identify not only single antibiotics in a wide level scope but also various combi-nations of two, three or even four antibiotics with good discrimination capacity. Differentiation of multiple antibiotics in river water, milk, and urine matrices was also demonstrated. Furthermore, over 90% accuracy was gained for unknown samples in double-blind tests. Our work offers a portable, low-cost, and high-performance tool to determine multiple antibiotics simultaneously, which will stimulate future exploration of hand-held sensor arrays with simple sensing elements for on-site analysis and point-of-care diagnosis.
作者机构：
[Tang, Peng; Wang, Yue; Yao, Xueqiong; Wang, Yangcan; Song, Fengmei; Deng, Shuxiang; Yang, Fei; Tang, Yan] Department of Epidemiology and Health Statistics, The Key Laboratory of Typical Environmental Pollution and Health Hazards of Hunan Province, School of Basic Medicine, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China;[Tang, Peng] Department of Epidemiology and Health Statistics, School of Public Health, Guangxi Medical University, Nanning, 530021, Guangxi, China;[Tang, Peng] Department of Maternal and Child Health, School of Public Health, Peking University, Beijing, 100191, China;[Liao, Qian] Department of Epidemiology and Health Statistics, School of Public Health, Guangxi Medical University, Nanning, 530021, Guangxi, China;[Du, Can] Hunan Provincial Key Laboratory of Clinical Epidemiology, Xiangya School of Public Health, Central South University, Changsha, 410078, China
通讯机构：
[Xiaoqiang Qiu; Fei Yang] D;Department of Epidemiology and Health Statistics, School of Public Health, Guangxi Medical University, Nanning, 530021, Guangxi, China<&wdkj&>Department of Epidemiology and Health Statistics, The Key Laboratory of Typical Environmental Pollution and Health Hazards of Hunan Province, School of Basic Medicine, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China<&wdkj&>Hunan Provincial Key Laboratory of Clinical Epidemiology, Xiangya School of Public Health, Central South University, Changsha, 410078, China
摘要：
BACKGROUND: Heavy metals entering the human body could cause damage to a variety of organs. However, the combined harmful effects of exposure to various metals on liver function are not well understood. The purpose of the study was to investigate the independent and joint relationships between heavy metal exposure and liver function in adults. METHODS: The study involved 3589 adults from the National Health and Nutrition Examination Survey. Concentrations of urinary metals, including arsenic (As), cadmium (Cd), lead (Pb), antimony (Sb), barium (Ba), thallium (Tl), tungsten (W), uranium (U), were determined in urine using inductively coupled plasma mass spectrometry. Data for liver function biomarkers included alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transaminase (GGT), and alkaline phosphatase (ALP). Survey-weighted linear regression and quantile g-computation (qgcomp) were employed to evaluate the relationship of urinary metals with the markers of liver injury. RESULTS: Cd, U and Ba were found to have positive correlations with ALT, AST, GGT, and ALP in the survey-weighted linear regression analyses. According to the qgcomp analyses, the total metal mixture was positively correlated with ALT (percent change: 8.15; 95% CI: 3.84, 12.64), AST (percent change: 5.55; 95% CI: 2.39, 8.82), GGT (percent change: 14.30; 95% CI: 7.81, 21.18), and ALP (percent change: 5.59; 95% CI: 2.65, 8.62), and Cd, U, and Ba were the main contributors to the combined effects. Positive joint effects were observed between Cd and U on ALT, AST, GGT and ALP, and U and Ba had positive joint effects on ALT, AST and GGT. CONCLUSION: Exposures to Cd, U, and Ba were individually associated with multiple markers of liver injury. Mixed metal exposure might be adversely correlated with markers of liver function. The findings indicated the potential harmful effect of metal exposure on liver function.
期刊：
Journal of Medical Virology,2023年95(5):e28797- ISSN：0146-6615
通讯作者：
Yang, ZR;Feng, SD
作者机构：
[Zheng, Chenli; Wang, Xiaohui; Gan, Yongxia; Zhao, Jin; Yang, Zhengrong; Zeng, Guang; Li, Guilian] Shenzhen Ctr Dis Control & Prevent, Dept HIV AIDS Prevent & Control, Shenzhen, Peoples R China.;[Zeng, Guang; Feng, Shuidong; Tang, Jie] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Peoples R China.;[He, Fei] Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Stomatol, Shenzhen, Peoples R China.;[Zhang, Xiaomin] Shenzhen Ctr Dis Control & Prevent, Inst Pathogen Biol, Shenzhen, Peoples R China.;[Xu, Liumei] Southern Univ Sci & Technol, Peoples Hosp Shenzhen 3, Affiliated Hosp 2, Natl Clin Res Ctr Infect Dis, Shenzhen, Peoples R China.
通讯机构：
[Yang, ZR ] S;[Feng, SD ] U;Shenzhen Ctr Dis Control & Prevent, Dept HIV AIDS Prevent & Control, Shenzhen, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Peoples R China.
摘要：
The immunogenicity induced by the third dose of inactivated coronavirus disease 2019 (COVID-19) vaccines in people living with HIV (PLWH) is unclear, and relevant literature is extremely scarce. It is important to add evidence on the humoral immune response induced by the third dose of inactivated COVID-19 vaccine in PLWH. We collected peripheral venous blood for spike receptor binding domain-protein specific immunoglobulin G (S-RBD-IgG) antibody tests at 28 days after the second dose (T(1) ), 180 days after the second dose (T(2) ) and 35 days after the third dose (T(3) ) of inactivated COVID-19 vaccines in PLWH. The differences in S-RBD-IgG antibody levels and specific seroprevalence among T(1) , T(2) , and T(3) time periods were analyzed, and the effects of age, vaccine brand, and CD4(+) T cell count on the levels and specific seroprevalence of S-RBD-IgG antibody induced by the third dose in PLWH were examined. The third dose of inactivated COVID-19 vaccines induced strong S-RBD-IgG antibody responses in PLWH. The levels and specific seroprevalence of S-RBD-IgG antibody were significantly higher than those at 28 and 180 days after the second dose and were not affected by vaccine brand or CD4(+) T cell count. Younger PLWH produced higher levels of S-RBD-IgG antibody. The third dose of inactivated COVID-19 vaccine showed good immunogenicity in PLWH. It is necessary to popularize the third dose in the PLWH population, especially PLWH who do not respond to two doses of inactivated COVID-19 vaccines. Meanwhile, the durability of the protection provided by the third dose in PLWH must be continuously monitored.
期刊：
FRONTIERS IN IMMUNOLOGY,2023年14:1138818 ISSN：1664-3224
作者机构：
[Cao, Bin; Yuan, Xiuqin; Yu, Jinjie] Univ South China, Sch Publ Hlth, Hengyang, Peoples R China.;[Qian, Chengyu; Cao, Bin; Zhao, Xiuqin; Wang, Ruihuan; Liu, Haican; Yu, Jinjie; Fan, Xueting; Luan, Xiuli; Li, Guilian; Li, Machao; Wan, Kanglin] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing, Peoples R China.;[Qian, Chengyu] Wenzhou Med Univ, Coll Lab Med, Sch Life Sci, Wenzhou, Peoples R China.
关键词：
Mycobacterium tuberculosis;ESAT-6;CFP-10;nPstS1;adjuvant;Recombinant subunit protein vaccine
摘要：
Tuberculosis (TB) is an infectious disease that seriously affects human health. Until now, the only anti-TB vaccine approved for use is the live attenuated Mycobacterium bovis (M. bovis) vaccine - BCG vaccine, but its protective efficacy is relatively low and does not provide satisfactory protection against TB in adults. Therefore, there is an urgent need for more effective vaccines to reduce the global TB epidemic. In this study, ESAT-6, CFP-10, two antigens full-length and the T-cell epitope polypeptide antigen of PstS1, named nPstS1, were selected to form one multi-component protein antigens, named ECP001, which include two types, one is a mixed protein antigen named ECP001m, the other is a fusion expression protein antigen named ECP001f, as candidates for protein subunit vaccines. were prepared by constructing one novel subunit vaccine by mixing or fusing the three proteins and combining them with aluminum hydroxide adjuvant, and the immunogenicity and protective properties of the vaccine was evaluated in mice. The results showed that ECP001 stimulated mice to produce high titre levels of IgG, IgG1 and IgG2a antibodies; meanwhile, high levels of IFN-gamma and a broad range of specific cytokines were secreted by mouse splenocytes; in addition, ECP001 inhibited the proliferation of Mycobacterium tuberculosis in vitro with a capacity comparable to that of BCG. It can be concluded that ECP001 is a novel effective multicomponent subunit vaccine candidate with potential as BCG Initial Immunisation-ECP001 Booster Immunisation or therapeutic vaccine for M. tuberculosis infection.
作者机构：
[Fang, Danang; Qian, Chengyu; Lou, Yongliang] Wenzhou Med Univ, Sch Lab Med & Life Sci, Wenzhou 325035, Peoples R China.;[Zhao, Xiuqin; Wang, Ruihuan; Liu, Haican; Jiang, Yi; Fan, Xueting; Luan, Xiuli; Qian, Chengyu; Li, Guilian; Li, Machao; Wan, Kanglin] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China.;[Yu, Jinjie; Cao, Bin] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Kanglin Wan; Haican Liu; Yongliang Lou] A;Authors to whom correspondence should be addressed.<&wdkj&>School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
摘要：
Tuberculosis (TB) remains a serious global health problem. Despite the widespread use of the Mycobacterium bovis bacillus Calmette-Guerin (BCG) vaccine, the primary factor for the TB pandemic and deaths is adult TB, which mainly result from endogenous reactivation of latent Mycobacterium tuberculosis (MTB) infection. Improved new TB vaccines with eligible safety and long-lasting protective efficacy remains a crucial step toward the prevention and control of TB. In this study, five immunodominant antigens, including three early secreted antigens and two latency associated antigens, were used to construct a single recombinant fusion protein (Epera013f) and a protein mixture (Epera013m). When formulated with aluminum adjuvant, the two subunit vaccines Epera013m and Epera013f were administered to BALB/c mice. The humoral immune responses, cellular responses and MTB growth inhibiting capacity elicited after Epera013m and Epera013f immunization were analyzed. In the present study, we demonstrated that both the Epera013f and Epera013m were capable of inducing a considerable immune response and protective efficacy against H37Rv infection compared with BCG groups. In addition, Epera013f generated a more comprehensive and balanced immune status, including Th1, Th2 and innate immune response, over Epera013f and BCG. The multistage antigen complex Epera013f possesses considerable immunogenicity and protective efficacy against MTB infection ex vivo indicating its potential and promising applications in further TB vaccine development.
摘要：
The beta-NaYF4:Yb,Tm,Gd/Ni-MOF (UCNR/Ni-MOF, UNM) nanocomposites were successfully synthesized via a simple two-step hydrothermal method. Excited by a 980 nm laser, UCNRs produce ultraviolet and visible light, activating the Ni-MOF and generating a significant quantity of electron/hole pairs (e(-)/h(+)). These e(-)/h(+) pairs can then react with O-2 and H2O to create reactive oxygen species (ROS), which can be used for antibacterial purposes. Characterization techniques including transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) were utilized to analyze the structure, optical properties, composition, and morphology of the UNM nanocomposites. The photocatalytic performance of UNM was evaluated by testing its ability to kill E. coli and S. aureus as well as degrade rhodamine B (RhB) under 980 nm near-infrared light irradiation (1.0 W/cm(2)). After 18 min of reaction, the bactericidal rates against E. coli and S. aureus were observed to be roughly 100 and 99.99%, respectively. Similarly, similar to 98.49% of RhB was degraded within 180 min. Free radical capture experiments were conducted to further investigate the mechanism of UNM photocatalysis. The main active species involved were determined to be <middle dot>O-2(-) and h(+). In addition, UNM was able to retain similar to 86.25% of its degradation rate toward RhB after four cycles of cycling experiments, which demonstrated its good stability. Therefore, this study provides a potential strategy to eliminate bacteria and degrade hazardous pollutants in order to mitigate environmental pollution.
