期刊论文

Cai, R;Huang, KJ;Yang, HF

英文

Chemical Engineering Journal

1385-8947

2023

468

143732

10.1016/j.cej.2023.143732

In summary, a “on–off” self-powered biosensor was designed for sequential ultra-sensitive detection of miRNA-21 and miRNA-155 with detection limits of 0.17 fM and 0.37 fM, respectively. The detection method of this biosensor was a target-controlled capture and release process of the single-bioanode enzyme. The miRNA-21 stabilized the ability of the DNA2-GOD to provide electronic donors and showed a “on” effect on the E1OCV. Meanwhile, the strong interaction between miRNA-155 and DNA1 destroyed

本校为通讯机构

A "on-off" self-powered biosensor was developed based on enzymatic biofuel cells (EBFCs) for sequential detection of microRNA-21 (miRNA-21) and miRNA-155 by capture and release of a single-bioanode enzyme, i.e., glucose oxidase. When miRNA-21 is present, it hybridizes with DNA1 and the glucose oxidase (GOD)-modified DNA2, i.e., DNA2-GOD. Then, GOD oxidizes glucose to produce a large number of electrons, and a significantly increased open-circuit voltage (E1OCV) is observed, corresponding to the "on" state. If miRNA-155 is present, it hybridizes with DNA1 and DNA3-functionalized SiO2 nanosphere...