期刊：
Dyes and Pigments,2023年211:111083 ISSN：0143-7208
通讯作者：
Li, S.;He, L.
作者机构：
[Wang, Peipei; Li, Songjiao] Univ South China, Ctr Mol Imaging Probe, Hunan Prov Key Lab Tumor Cellular & Mol Pathol, Hengyang Med Sch,Canc Res Inst, Hengyang 421001, Hunan, Peoples R China.;[Liu, Ying; Yang, Ke; He, Longwei; Li, Songjiao] Univ South China, Hengyang Med Sch, Dept Pharm & Pharmacol, Hengyang, Peoples R China.;[Cheng, Dan] Univ South China, Affiliated Nanhua Hosp, Clin Res Inst, Hengyang Med Sch, Hengyang, Peoples R China.
通讯机构：
[Songjiao Li] C;[Longwei He] D;Center for Molecular Imaging Probe, Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China<&wdkj&>Department of Pharmacy and Pharmacology, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>Department of Pharmacy and Pharmacology, Hengyang Medical School, University of South China, Hengyang, China
摘要：
A near-infrared methylene blue-based fluorogenic probe with an on/off activation ability has emerged as an important means to detect and monitor biological analytes and to understand the biological processes involved in understanding these analytes within cells and organisms. By using biomarker-activated methylene blue-based probes, it is possible to produce more precise results with a lower risk of photodamage to biological samples and fewer biological interferences in the background. This brief review aims to give a rigorous but concise account of recent developments in activatable methylene blue-based fluorogenic probes by reporting the significant accomplishments in that field. Several strategies for detecting and imaging disease-related biomarkers (such as ROS, RRS, enzymes, ions, and other related species) have been discussed and analyzed. Additionally, methylene blue-based fluoroscopic probes are emphasized for use in developing new methods to detect and image biomarkers to address potential challenges and opportunities.
摘要：
The histone deacetylase HDAC3 is associated with the NCoR/SMRT co-repressor complex, and its canonical function is in transcriptional repression, but it can also activate transcription. Here, we show that the repressor and activator functions of HDAC3 can be genetically separated in Drosophila. A lysine substitution in the N terminus (K26A) disrupts its catalytic activity and activator function, whereas a combination of substitutions (HEBI) abrogating the interaction with SMRTER enhances repressor activity beyond wild type in the early embryo. We conclude that the crucial functions of HDAC3 in embryo development involve catalytic-dependent gene activation and non-enzymatic repression by several mechanisms, including tethering of loci to the nuclear periphery.
通讯机构：
[Zhizhong Xie] I;Institute of Pharmacy and Pharmacology, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, University of South China, Hengyang, China
摘要：
Dysmenorrhea is a prevalent gynecological disease among women at reproductive age. It is classified as the primary dysmenorrhea and the secondary dysmenorrhea according to the etiology. The primary dysmenorrhea is caused by uterine hypercontraction without any identifiable pelvic lesions, while the secondary dysmenorrhea is incurred by gynecological disorder with pelvic organic lesions. However, the underlying mechanism of dysmenorrhea is not completely clear. Animal models of dysmenorrhea, especially mouse and rat model, are helpful to explore the pathophysiological mechanism of dysmenorrhea, clarify the therapeutic effect of compounds, and guide clinical treatment. The murine model of primary dysmenorrhea is commonly induced by oxytocin or prostaglandin F(2α), while the secondary dysmenorrhea murine model was further created by injecting oxytocin on the basis of the established primary disease model. This review summarizes the current progress of dysmenorrhea models in rodent, including experimental methods, corresponding evaluation indexes, and the advantages and disadvantages of various murine dysmenorrhea models, in order to provide a reference for the selection of murine dysmenorrhea models and the further study of the pathophysiological mechanism of dysmenorrhea.
期刊：
INTERNATIONAL JOURNAL OF CLINICAL PHARMACOLOGY AND THERAPEUTICS,2023年61(3):129-138 ISSN：0946-1965
作者机构：
[Long, Hui-Zhi; Cheng, Yan; Xu, Shuo-Guo; Deng, Ping; Luo, Hong-Yu; Gao, Li-Chen; Li, Feng-Jiao; Zhou, Zi-Wei; Wen, Dan -Dan] Univ South China, Changsha Cent Hosp, Sch Pharm, Phase Clin Trial Ctr 1,Dept Pharm, Hengyang, Peoples R China.;[Long, Hui-Zhi; Cheng, Yan; Xu, Shuo-Guo; Deng, Ping; Luo, Hong-Yu; Gao, Li-Chen; Li, Feng-Jiao; Zhou, Zi-Wei; Wen, Dan -Dan] Hunan Prov Key Lab Tumor Microenvironm Respons Dru, Changsha, Peoples R China.;[Yao, Zhen-Jiang; Guan, Yue-Qing] Ningbo High Tech Zone Minova Pharmaceut Innovat Re, Ningbo, Peoples R China.;[Gao, Li-Chen] Univ South China, Changsha Cent Hosp, Sch Pharm, Dept Pharm,Phase Clin Trial Ctr 1, Changsha 410004, Peoples R China.
关键词：
apixaban;bioequivalence;pharmacokinetics;safety-;safety- fasting and fed conditions
摘要：
Objective: To evaluate the pharmacokinetics (PK), safety, and bioequiv-alence of two formulations of apixaban in healthy Chinese subjects under fasting and fed conditions. Materials and methods: A single-center, randomized, open, single-dose, two-period crossover PK study was carried out under fasting and fed conditions in 64 healthy subjects enrolled in either the fasting (36 subjects) or the fed (28 subjects) arms of the study. Subjects received a sin-gle oral dose of 2.5 mg apixaban tablets as test (T) or reference (R) formulation. The primary PK parameters determined were the area under the plasma concentration-time curve from zero to t and infinity (AUC0-t and AUC0-infinity) and the maximal plasma concentra-tion (Cmax). Safety was assessed mainly from the occurrence of adverse events (AEs). Results: A single drop-out in the fed arm of the trial was excluded from the statistical evaluation. The 90% confidence intervals (CIs) for the geometric mean ratio (GMR) for T/R using AUC0-t were 95.4 - 100.9% and 97.8 - 103.8%, and for AUC0-infinity were 95.3 - 100.6% and 98.3 - 104.3% under fast -ing (36 subjects) and fed (27 subjects) condi-tions, respectively. Similarly, the 90% CIs for Cmax were 94.6 - 103.1% and 88.8 - 102.0% under fasting (36 subjects) and the fed (27 subjects) conditions, respectively. There-fore, the 90% CIs for the T/R AUC and Cmax ra -tios were within the standard range for bio-equivalence (80.0 - 125.0%). There were no serious adverse events (SAEs). Conclusion: The test and reference 2.5 mg apixaban tab-lets were bioequivalent and both showed good tolerability and safety.
通讯机构：
[Yunmei Liu] I;Institute of Pharmacy & Pharmacology, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China
关键词：
Chrysin;3D-QSAR;CoMFA;CoMSIA
摘要：
In this paper, 3D-QSAR models were constructed to conduct a preliminary study on the structure-activity relationship of chrysin. The three-dimensional structures of the selected 54 chrysin derivatives were constructed by SYBYL-X 2.0 software, molecular mechanics procedures for conformational optimization, and molecular alignment. The 3D-QSAR model of these compounds was constructed by comparative molecular force field analysis (CoMFA) and comparative molecular similarity index analysis (CoMSIA), and the structure-activity relationships of chrysin compounds was preliminarily discussed. CoMFA and CoMSIA models are all constructed reasonably and reliably (CoMFA: q(2) = 0.703, r(2) = 0.886, SEE = 0.217, F = 113.572; CoMSIA: q(2) = 0.647, r(2) = 0.841, SEE = 0.255, F = 111.740), a series of three-dimensional contour maps were obtained to visualize the influence of various fields around the compound molecules on the drug activity. Groups with more hydrogen bonding acceptors attached to the end of the 7-O-alkane chain of chrysin are favorable for the molecular activity, such as amino acids. However, excessively long 7-O-alkane chains or the introduction of bulky hydrophobic groups on the 7-O-alkane chain will reduce the activity of the molecule. In contrast, the introduction of bulky hydrophobic groups on the side chains of amino acids will enhance the molecular activity.
期刊：
CLINICAL AND EXPERIMENTAL MEDICINE,2023年23(7):3113-3124 ISSN：1591-8890
通讯作者：
She, MH
作者机构：
[Zeng, Qun; Zhang, Jiawei; She, Meihua; She, MH] Univ South China, Hengyang Med Sch, Dept Biochem & Mol Biol, Changsheng West Rd 28, Hengyang 421001, Peoples R China.
通讯机构：
[She, MH ] U;Univ South China, Hengyang Med Sch, Dept Biochem & Mol Biol, Changsheng West Rd 28, Hengyang 421001, Peoples R China.
关键词：
Four and a half LIM protein 2 (FHL2);LIM-only protein family;Tumor progression;Cancer treatment;Signaling pathways
摘要：
LIM domain protein 2, also known as LIM protein FHL2, is a member of the LIM-only family. Due to its LIM domain protein characteristics, FHL2 is capable of interacting with various proteins and plays a crucial role in regulating gene expression, cell growth, and signal transduction in muscle and cardiac tissue. In recent years, mounting evidence has indicated that the FHLs protein family is closely associated with the development and occurrence of human tumors. On the one hand, FHL2 acts as a tumor suppressor by down-regulating in tumor tissue and effectively inhibiting tumor development by limiting cell proliferation. On the other hand, FHL2 serves as an oncoprotein by up-regulating in tumor tissue and binding to multiple transcription factors to suppress cell apoptosis, stimulate cell proliferation and migration, and promote tumor progression. Therefore, FHL2 is considered a double-edged sword in tumors with independent and complex functions. This article reviews the role of FHL2 in tumor occurrence and development, discusses FHL2 interaction with other proteins and transcription factors, and its involvement in multiple cell signaling pathways. Finally, the clinical significance of FHL2 as a potential target in tumor therapy is examined.
作者机构：
[Zhang, Taolan; Li, Zhuo; Hu, Haihong; Zhan, Wendi; Lei, Xiaoyong; Zhu, Hongxia; Tang, Liyang] Univ South China, Hengyang Med Coll, Sch Pharm, 28 Western Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.;[Zhang, Taolan; Li, Zhuo; Hu, Haihong; Zhan, Wendi; Zhang, TL; Zhu, Hongxia; Tang, Liyang] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Pharm, 69 Chuanshan Rd, Hengyang 421001, Hunan, Peoples R China.;[Zhang, Taolan; Li, Zhuo; Hu, Haihong; Zhan, Wendi; Zhu, Hongxia; Tang, Liyang] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Chinese Tradit Med TCM Res Platform Major Epidem T, 69 Chuanshan Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Zhang, TL ] U;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Pharm, 69 Chuanshan Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
Breast cancer;Fatty acid metabolism;Unsupervised clustering;Tumor immune microenvironment;Immunotherapy
摘要：
Breast cancer (BRCA) is a major global health issue, characterized by high mortality and low early diagnosis rates. The tumor immune microenvironment (TME) of BRCA is closely linked to fatty acid metabolism (FAM). This study aimed to identify FAM-related subtypes in BRCA based on gene expression and clinical data from the Cancer Genome Atlas (TCGA) database. The study found two distinct FAM-related subtypes, each with unique immune characteristics and prognostic implications. A FAM-related risk score prognostic model was developed and validated using TCGA and International Cancer Genome Consortium (GEO) cohorts, showing potential clinical applications for chemotherapy and immunotherapy. Additionally, a nomogram was established to facilitate clinical use of the risk score. These results highlight the significant correlation between FAM genes and TME in BRCA, and demonstrate the potential clinical utility of the FAM-related risk score in informing treatment decisions for BRCA patients.
期刊：
BRIEFINGS IN BIOINFORMATICS,2023年24(6) ISSN：1467-5463
通讯作者：
Chen, YP;Lin, WB
作者机构：
[Li, Zhe; Tu, Xinyi] Univ South China, Sch Comp, Hengyang, Peoples R China.;[Chen, Yuping; Chen, YP] Univ South China, Sch Pharm, Hengyang 421001, Peoples R China.;[Lin, Wenbin; Lin, WB] Univ South China, Sch Math & Phys, Hengyang 421001, Peoples R China.
通讯机构：
[Lin, WB ; Chen, YP ] U;Univ South China, Sch Pharm, Hengyang 421001, Peoples R China.;Univ South China, Sch Math & Phys, Hengyang 421001, Peoples R China.
关键词：
drug–drug interaction;pre-taining;heterogeneous graph neural network;multi-source information
摘要：
The simultaneous use of two or more drugs due to multi-disease comorbidity continues to increase, which may cause adverse reactions between drugs that seriously threaten public health. Therefore, the prediction of drug-drug interaction (DDI) has become a hot topic not only in clinics but also in bioinformatics. In this study, we propose a novel pre-trained heterogeneous graph neural network (HGNN) model named HetDDI, which aggregates the structural information in drug molecule graphs and rich semantic information in biomedical knowledge graph to predict DDIs. In HetDDI, we first initialize the parameters of the model with different pre-training methods. Then we apply the pre-trained HGNN to learn the feature representation of drugs from multi-source heterogeneous information, which can more effectively utilize drugs' internal structure and abundant external biomedical knowledge, thus leading to better DDI prediction. We evaluate our model on three DDI prediction tasks (binary-class, multi-class and multi-label) with three datasets and further assess its performance on three scenarios (S1, S2 and S3). The results show that the accuracy of HetDDI can achieve 98.82% in the binary-class task, 98.13% in the multi-class task and 96.66% in the multi-label one on S1, which outperforms the state-of-the-art methods by at least 2%. On S2 and S3, our method also achieves exciting performance. Furthermore, the case studies confirm that our model performs well in predicting unknown DDIs. Source codes are available at https://github.com/LinsLab/HetDDI.
摘要：
This study presents the first-ever synthesis of samarium-doped indium vanadate nanosheets (IVONSs:Sm) via microemulsion-mediated solvothermal method. The nanosheets were subsequently utilized as a nano-matrix in laser desorption/ionization mass spectrometry (LDI-MS). It was discovered that the as-synthesized IVONSs:Sm possessed the following advantages: improved mass spectrometry signal, minimal matrix-related background, and exceptional stability in negative-ion mode. These qualities overcame the limitations of conventional matrices and enabled the sensitive detection of small biomolecules such as fatty acids. The negative-ion LDI mechanism of IVONSs:Sm was examined through the implementation of density functional theory simulation. Using IVONSs:Sm-assisted LDI-MS, fingerprint recognitions based on morphology and chemical profiles of endogenous/exogenous compounds were also achieved. Notably, crucial characteristics such as the age of an individual’s fingerprints and their physical state could be assessed through the longitudinal monitoring of particular biomolecules (e.g., ascorbic acid, fatty acid) or the specific biomarker bilirubin glucuronide. Critical information pertinent to the identification of an individual would thus be facilitated by the analysis of the compounds underlying the fingerprint patterns.
摘要：
Photodynamic therapy (PDT) is attractive in cancer treatment because of its non-invasive and low side effects. In this study, we synthesized 20 porphyrin-coumarin compounds via condensation reactions of porphyrins with coumarin derivatives. In the singlet oxygen (1O2) assay, DPBF fluorescence was quenched by 85% after 10 s of light irradiation; the fluorescence of DPBF almost disappeared at 30 s by light irradiation. This indicates that the porphyrin-coumarin compounds have excellent 1O2 generation efficiency. Moreover, porphyrin-coumarin compounds showed different inhibitory effects on A549 and HepG2 cells under light irradiation. Whereas in dark conditions, compounds with shorter alkyl chains showed antitumor activity, the extension of branched and alkyl chains decreased the activity of the compounds. Therefore, accessing coumarin derivatives with shorter alkyl chains to porphyrins achieves PDT and chemotherapy. Additionally, the insertion of metal Zn into porphyrins also enhances the PDT effect of the compounds.
期刊：
JOURNAL OF CELLULAR BIOCHEMISTRY,2023年124(4):586-605 ISSN：0730-2312
通讯作者：
Haoliang Hu<&wdkj&>Lanfang Li<&wdkj&>Linxi Chen<&wdkj&>Haoliang Hu Haoliang Hu Haoliang Hu<&wdkj&>Lanfang Li Lanfang Li Lanfang Li<&wdkj&>Linxi Chen Linxi Chen Linxi Chen
作者机构：
[Huang, Shifang; Luo, Xuling; Jiang, Jinyong; Huang, Zhen; Liu, Meiqing; Hu, Haoliang; Li, Yao; Li, Lanfang; Wang, Lingzhi; He, Lu; Chen, Zhe; Cao, Jiangang; Chen, Linxi] Univ South China, Inst Pharm & Pharmacol, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Hengyang Med Sch,Hunan Prov Key Lab tumor Microenv, Hengyang, Peoples R China.;[Hu, Haoliang] Hunan Univ Arts & Sci, Coll Life & Environm Sci, Changde Res Ctr Artificial Intelligence & Biomed, Changde, Hunan, Peoples R China.;[Hu, Haoliang; Li, Lanfang; Chen, Linxi] Univ South China, Inst Pharm & Pharmacol, Coll Basic Med Sci, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Hengyang 421001, Peoples R China.
通讯机构：
[Haoliang Hu; Lanfang Li; Linxi Chen; Haoliang Hu Haoliang Hu Haoliang Hu; Lanfang Li Lanfang Li Lanfang Li; Linxi Chen Linxi Chen Linxi Chen] H;Hunan Provincial Key Laboratory of tumor microenvironment responsive drug research, Hengyang Medical School, Institute of Pharmacy and Pharmacology, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China<&wdkj&>Changde Research Center for Artificial Intelligence and Biomedicine, College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde, Hunan, China<&wdkj&>Hunan Provincial Key Laboratory of tumor microenvironment responsive drug research, Hengyang Medical School, Institute of Pharmacy and Pharmacology, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China
摘要：
Abstract The elabela‐apelin/angiotensin domain type 1 receptor‐associated protein (APJ) system is an important regulator in certain thrombosis‐related diseases such as atherosclerosis, myocardial infarction, and cerebral infarction. Our previous reports have revealed that apelin exacerbates atherosclerotic lesions. However, the relationship between the elabela‐apelin/APJ system and platelet aggregation and atherothrombosis is unclear. The results of the present study demonstrate that elabela and other endogenous ligands such as apelin‐12, ‐17, and ‐36 induce platelet aggregation and thrombosis by activating the pannexin1(PANX1)‐P2X7 signaling pathway. Interestingly, the diuretic, spironolactone, a novel PANX1 inhibitor, alleviated elabela‐ and apelin isoforms‐induced platelet aggregation and thrombosis. Significantly, two potential antithrombotic drugs were screened out by targeting APJ receptors, including the anti‐HIV ancillary drug cobicistat and the traditional Chinese medicine monomer Schisandrin A. Both cobicistat and Schisandrin A abolished the effects of elabela and apelin isoforms on platelet aggregation, thrombosis, and cerebral infarction. In addition, cobicistat significantly attenuated thrombosis in a ponatinib‐induced zebrafish trunk model. Overall, the elabela–apelin/APJ axis mediated platelet aggregation and thrombosis via the PANX1‐P2X7 signaling pathway in vitro and in vivo. Blocking the APJ receptor with cobicistat/Schisandrin A or inhibiting PANX1 with spironolactone may provide novel therapeutic strategies against thrombosis.
通讯机构：
[Cui-Yun Yu; Hua Wei; Cui-Yun Yu Cui-Yun Yu Cui-Yun Yu; Hua Wei Hua Wei Hua Wei] H;Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study & School of Pharmaceutical Science, Hengyang Medical School, University of South China, 28 W Changsheng Road, Hengyang, Hunan, 421001 China
关键词：
angiogenesis;injectable hydrogels;mitochondrial targeting;myocardial infarction;reactive oxygen species scavenging
摘要：
Mitochondrial dysfunction of cardiomyocytes (CMs) has been identified as a significant pathogenesis of early myocardial infarction (MI). However, only a few agents or strategies have been developed to improve mitochondrial dysfunction for the effective MI treatment. Herein, a reactive oxygen species (ROS)-responsive PAMB-G-TK/4-arm-PEG-SG hydrogel is developed for localized drug-loaded liposome delivery. Notably, the liposomes contain both elamipretide (SS-31) and sphingosine-1-phosphate (S1P), where SS-31 acts as an inhibitor of mitochondrial oxidative damage and S1P as a signaling molecule for activating angiogenesis. Liposome-encapsulated PAMB-G-TK/4-arm-PEG-SG hydrogels demonstrate myocardium-like mechanical strength and electrical conductivity, and ROS-sensitive release of SS-31 and S1P-loaded liposomes. Further liposomal release of SS-31, which can target cytochrome c in the mitochondrial inner membrane of damaged CMs, inhibits pathological ROS production, improving mitochondrial dysfunction. Meanwhile, S1P released from the liposome induces endothelial cell angiogenesis by activating the S1PR1/PI3K/Akt pathway. In a rat MI model, the resulting liposomal composite hydrogel improves cardiac function by scavenging excess ROS, improving mitochondrial dysfunction, and promoting angiogenesis. This study reports for the first time a liposomal composite hydrogel that can directly target mitochondria of damaged CMs for a feedback-regulated release of encapsulated liposomes to consume the overproduced pathological ROS for improved CM activity and enhanced MI treatment.
摘要：
Multi-drug resistance (MDR) is characterized by the resistance of tumor cells to some antitumor drugs with different structures and mechanisms after the use of a single chemotherapy drug or even the first use of the drug. Notably, MDR has become the largest obstacle to the success of gastric cancer chemotherapies. Non-coding RNAs are defined as a class of RNAs that do not have the ability to code proteins. They are widely involved in important biological functions in life activities. Multiple lines of evidence demonstrated that ncRNAs are closely related to human cancers, including gastric cancer. However, the relationship between ncRNAs and MDR in gastric cancer has been reported, yet the mechanisms are not fully clarified. Therefore, in this review, we systematically summarized the detailed molecular mechanisms of lncRNAs (long noncoding RNAs) and miRNAs (microRNAs) associated with MDR in gastric cancer. Additionally, we speculate that the abnormal expression of ncRNAs is likely to be a novel potential therapeutic target reversing MDR for gastric cancer. Future therapeutics for gastric cancer will most likely be based on noncoding RNAs (ncRNAs) that regulate MDR-related genes.
通讯机构：
[Shengsong Tang] H;Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, and Institute of Pharmacy and Pharmacology, University of South China , Hengyang421001 , China<&wdkj&>Hunan Province Key Laboratory for Antibody-Based Drug and Intelligent Delivery System, School of Pharmaceutical Sciences, Hunan University of Medicine , Huaihua418000 , China<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University , Changsha 410128 , China
摘要：
Extrachromosomal DNA, referred to as extrachromosomal DNA (ecDNA), was found in most cancers and nearly absent in normal cells. The properties of ecDNA enable tumor cells to be more responsive to various environments. The non-Mendelian genetic mechanism of ecDNA could arouse increasing tumor heterogeneity. Besides, ecDNA would promote tumor invasiveness and provide resistance mechanisms associated with poorer survival consequences. Furthermore, ecDNA could profoundly impact oncogene activation, genome instability, tumor heterogeneity, etc. Consequently, they may offer potential possibilities for tumor diagnosis and therapeutics. We primarily reviewed the classification, several primary formation mechanisms, homeostasis maintenance and frontier progress of ecDNA and late emphasized its fundamental roles in tumorigenesis and put forward some new insights. We identified that miR-3168 was increased in liver CSCs and inhibited liver CSC expansion via directly targeting p53. We also illustrated that miR-3168 may predict survival benefit from TACE in HCC patients.
作者机构：
[Wan, Teng; Mei, Zubing; Tan, Haifeng; Cao, Qi; Guo, Weiming; Xiao, Zhihong; Chen, Liangyuan; Tang, Guojun] Univ South China, Affiliated Hosp 2, Dept Spine Surg, Hengyang, Hunan, Peoples R China.;[Wan, Teng; Tan, Haifeng] Univ South China, Hengyang Med Coll, Dept Physiol, Hengyang, Hunan, Peoples R China.;[Wan, Teng; Tan, Haifeng] Univ South China, Hengyang Med Coll, Dept Biochem, Hengyang, Hunan, Peoples R China.;[Wang, Xitao] Binhai New Area Hosp Tradit Chinese Med, Dept Surg, Tianjin, Peoples R China.;[Mei, Zubing] Shanghai Univ Tradit Chinese Med, Shuguang Hosp, Dept Anorectal Surg, Shanghai, Peoples R China.
摘要：
BACKGROUND: Sarcopenia is a progressive age-related skeletal muscle disorder characterized by decreased muscle mass and loss of muscle function. Recent studies have shown that sarcopenia is able to predict a variety of clinical outcomes after spinal surgery. Controversy still exists among previous reports in terms of the definition and measurement of sarcopenia, these findings are heterogeneous so far. Therefore, the aim of the current study is to assess the up-to-date evidence of sarcopenia for postoperative outcomes among people undergoing spinal surgery. METHODS AND ANALYSIS: This protocol was carried out based on the preferred reporting items for systematic review and meta-analysis protocols (PRISMA-P) statement. It has been pre-registered in PROSPERO with the registration number of CRD42021260459. Three databases (including Pubmed, EMBASE, and Cochrane Library) will be searched from inception through May 10, 2021 to determine related cohort studies examining sarcopenia on multidimensional outcomes in patients undergoing spinal surgery. Major outcomes will be involved including mortality, morbidity, length of stay, postoperative complications or adverse events. DerSimonian & Laird random-effects meta-analysis will be used to calculate pooled odds ratio (OR) for binary data and pooled weighted mean differences (WMDs) or standardized mean differences (SMDs) for continuous data. The Newcastle-Ottawa Scale (NOS) will be used to assess the risk of bias of included studies. Narrative synthesis will be carried out if a pooled analysis is not possible. ETHICS AND DISSEMINATION: Ethical approval is not required for this study as the data involved are from the published literatures. We intend to disseminate or share the results of the study in a peer-reviewed journal or at relevant conferences. PROSPERO REGISTRATION NUMBER: CRD42021260459.
作者机构：
[He, LW; Zeng, Jiayu; Yang, Ke; He, Longwei; Li, Songjiao] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang Med Sch, Canc Res Inst,Dept Pharm & Pharmacol, Hengyang, Peoples R China.;[Cheng, Dan; Xia, Yuqing] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Clin Res Inst, Hengyang, Peoples R China.;[Cheng, Dan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha, Peoples R China.
通讯机构：
[He, LW ; Cheng, D ] U;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang Med Sch, Canc Res Inst,Dept Pharm & Pharmacol, Hengyang, Peoples R China.;Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Clin Res Inst, Hengyang, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha, Peoples R China.
摘要：
Carbon monoxide (CO) is regarded as one of the most important gaseous transmitters, playing a vital role in biological systems; meanwhile, abnormal levels of CO can be correlated with conditions such as lung disease, Alzheimer's disease, and cardiovascular disease. CO-releasing molecules (CORMs) are chemical agents used to release CO as an endogenous, biologically active molecule in order to treat diseases. CO-releasing molecule-3 (CORM-3), as a convenient and safe CO donor and therapeutic drug molecule, has been widely used to release exogenous CO in living cells to study the physiological and pathological roles of CO in living systems. Herein, we designed a NIR-emitting probe (NIR-CORM-3) with a large Stokes shift based on a 4-(dimethylamino)cinnamaldehyde lepidine derived fluorophore. A 4-nitrobenzyl group was selected as the CORM-3 recognizing moiety, and the probe is able to selectively and sensitively respond to CORM-3 (within only 15 min). Upon encountering CORM-3, NIR-CORM-3 releases a fluorophore with a response at 670 nm, and it shows a remarkable Stokes shift (up to 250 nm). In addition, NIR-CORM-3 has low cytotoxicity and exhibits outstanding NIR imaging abilities in living cells and mice.
作者机构：
[Ma, Q.; Liu, H.; Liu, Y.; Guo, Z.] Univ South China, Sch Nursing, Hengyang, Peoples R China.;[Guo, Z.] Univ South China, Inst Pharm & Pharmacol, Hengyang, Peoples R China.
通讯机构：
[Z. Guo] S;School of Nursing, University of South China, Hengyang, China<&wdkj&>Institute of Pharmacy and Pharmacology, University of South China, Hengyang, China
摘要：
Objective: This study aimed to systematically clarify attitudes and influencing factors of the public toward COVID-19 vaccination for children or adolescents. Study design: This was a scoping review. Methods: This scoping review screened, included, sorted, and analyzed relevant studies on COVID-19 vaccination for children or adolescents before December 31, 2021, in databases, including PubMed, Elsevier, Web of Science, Cochrane Library, and Wiley. Results: A total of 34 studies were included. The results showed that the public's acceptance rate toward COVID-19 vaccination for children or adolescents ranged from 4.9% (southeast Nigerian mothers) to 91% (Brazilian parents). Parents' or adolescents' age, gender, education level, and cognition and behavior characteristics for the vaccines were the central factors affecting vaccination. The vaccine's safety, effectiveness, and potential side-effects were the main reasons affecting vaccination. Conclusions: Realizing current public attitudes of COVID-19 vaccination for adolescents or children can effectively develop intervention measures and control the pandemic as soon as possible through herd immunity. (c) 2022 The Royal Society for Public Health. Published by Elsevier Ltd. All rights reserved.
