期刊：
Clinical and Experimental Pharmacology and Physiology,2009年36(9):e32-e39 ISSN：0305-1870
通讯作者：
Liao, Duan-Fang
作者机构：
[Ling, Hong-Yan; Liao, Duan-Fang] Cent S Univ, Dept Pharmacol, Sch Pharmaceut Sci, Changsha, Hunan, Peoples R China.;[Tang, Cao-Ke; Zhang, Liang; Yin, Wei-Dong; Liao, Duan-Fang] Univ S China, Res Ctr Life Sci, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.;[Chen, Lin-Xi; Gao, Zhi-Ping; Zhang, Xiao-Ying; Zhu, Bing-Yang; Ou, He-Sheng; Ling, Hong-Yan; Tuo, Qin-Hui; Liao, Duan-Fang] Univ S China, Res Ctr Life Sci, Key Lab Pharmacoprote Hunan Prov, Inst Pharm & Pharmacol, Hengyang 421001, Hunan, Peoples R China.;[Ling, Hong-Yan] Univ S China, Res Ctr Life Sci, Dept Physiol, Sch Med, Hengyang 421001, Hunan, Peoples R China.;[Feng, Shui-Dong] Univ S China, Res Ctr Life Sci, Sch Publ Hlth, Dept Epidemiol, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, Duan-Fang] U;Univ S China, Res Ctr Life Sci, Key Lab Atherosclerol Hunan Prov, 28 W Chengsheng Rd, Hengyang 421001, Hunan, Peoples R China.
摘要：
Evidences indicate that a complex relationship exists among sleep disorders, obesity and insulin resistance. NEU-P11 is a novel melatonin agonist used in treatment of psychophysiological insomnia, and in animal studies NEU-P11 showed sleep-promoting effect. In this study, we applied NEU-P11 on obese rats to assess its potential melatoninergic effects in vivo. Obese models were established using high-fat/high-sucrose-fed for 5 months. NEU-P11 (10 mg/kg)/melatonin (4 mg/kg)/vehicle were administered by a daily intraperitoneal injection respectively for 8 weeks. Our results showed that NEU-P11 or melatonin inhibited both body weight gain and deposit of abdominal fat with no influence on food intake. The impaired insulin sensitivity and antioxidative potency were improved and the levels of plasma glucose, total cholesterol (TC), triglycerides (TG) decreased with an increased in HDL-cholesterol (HDL-c) after NEU-P11 or melatonin administration. These data suggest that NEU-P11, like melatonin, decreased body weight gain and improved insulin sensitivity and metabolic profiles in obese rats. We conclude that NEU-P11 has a melatoninergic effect on regulating body weight in obese rats and also improving metabolic profiles and efficiently enhancing insulin sensitivity. (C) 2009 Elsevier Ltd. All rights reserved.
摘要：
Sox genes share a highly conserved DNA-binding motif, the HMG (high mobility group)-box domain, and have diverse roles in vertebrate embryonic development. A novel SRY-related cDNA (temporarily called Sox33) isolated from the Chinese alligator (Alligator sinensis) is 1,819 bp in length, with an open reading frame from 220 to 1113 bp, encoding a protein of 298 amino acids. Two putative polyadenylation signal sequences (AATAAA) are present upstream of the poly(A) tail in the 3' UTR (at 1255-1260 and 1774-1779). The putative protein contains an HMG-box domain most closely related to hSox12, mSox4, rtSox11, and mSox11 homologs, indicating that alligator Sox33 belongs to group C in the Sox gene family. Alligator adult and developing tissues were tested for Sox33 mRNA by independent Northern blots using a 336-bp probe (at 907-1243) between the HMG-box and the poly(A) site I and a 277-bp probe (at 1477-1754) between the two polyadenylation sites. Two transcripts (1.3 kb and 1.8 kb) in developing brain and one (1.8 kb) in adult brain were identified by the 336-bp probe; only one transcript (1.8 kb) in developing and adult brains was detected by the 277-bp probe. The results suggest that alligator Sox33 may use a different polyadenylation mechanism in the developing brain and play a role in the development and maintenance of the nervous system.
期刊：
Journal of Pharmaceutical and Biomedical Analysis,2009年49(4):1123-1127 ISSN：0731-7085
通讯作者：
Chen, Bo
作者机构：
[Tian, Qingqing; He, Dongxiu; Yao, Shouzhou; Chen, Bo] Hunan Normal Univ, Minist Educ, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Peoples R China.;[He, Dongxiu] Univ S China, Sch Life Sci & Technol, Hengyang 421001, Peoples R China.
通讯机构：
[Chen, Bo] H;Hunan Normal Univ, Minist Educ, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Peoples R China.
关键词：
Anthraquinones;HPLC-fluorescence detection;HPLC-UV detection;Traditional Chinese medicine;Pharmaceutical preparation
摘要：
A reversed-phase high-performance liquid chromatography (RP-HPLC) method with fluorescence detection for simultaneous determination of five anthraquinones in Rhubarb collected from nine different locations in China, Polygonum cuspidatum, Polygoni multiflori and three pharmaceutical preparations is proposed and validated. Chromatography was carried out at 25 degrees C on a Hypersil C18 column with the isocratic mobile phase of methanol-0.1% aqueous formic acid (85:15, v/v) at a flow rate of 1.0 ml/min. The excitation and emission wavelengths were set at 440 and 540 nm, respectively. A comprehensive validation of the method included tests of sensitivity, linearity, precision and accuracy. The linear regressions were acquired with r > 0.999. Satisfactory intra- and inter-day precisions were achieved with R.S.D.s less than 3.95% and the average recovery factors obtained were in the range of 93.2-103.8%. (C) 2009 Elsevier B.V. All rights reserved.
摘要：
AIM: To investigate the effects of macrophage migration inhibitory factor (MIF) on proliferation of human gastric cancer MGC-803 cells and expression of cyclin D1 and p27~(Kip1) in them, and further determine whether the effects are related to the PI3K/Akt signal transduction pathway. METHODS: Gastric cancer MGC-803 cells were cultured and then treated with 50 μg/L recombinant human MIF (rhMIF) with and without a PI3K inhibitor, LY294002 (25 μmol/L). MTT assay was used to detect the proliferation of MGC-803 cells. Cell cycle was detected by flow cytometry. Expression of cyclin D1 and p27~(Kip1) mRNA was by reverse transcription-polymerase chain reaction. Protein expression of phosphorylated Akt (p-Akt), Akt, cyclin D1 and p27~(Kip1) was examined by immunocytochemistry and Western blotting. RESULTS: rhMIF significantly stimulated the proliferation of MGC-803 cells and cell cycle progression from G1 phase to S phase in a concentration- and time-dependent manner. After the MGC-803 cells were treated with rhMIF for 24 h, the expression of cyclin D1 was significantly up-regulated compared with the cells not treated with rhMIF at both mRNA and protein levels (0.97± 0.02 vs 0.74±0.01,P = 0.002;0.98±0.05 vs 0.69±0.04,P =0.003). The p27~(Kip1) was downregulated but only statistically significant at the protein level. rhMIF significantly increased the expression of p-Akt, which reached the peak at 30 min, but did not affect the expression of Akt. However, LY294002 inhibited all the effects of rhMIF. CONCLUSION: Macrophage MIF increases the proliferation of gastric cancer cells, induces the expression of cyclin D1 at the transcriptional level and inhibits the expression of p27~(Kip1) at the post-transcriptional level via the PI3K/Akt pathway.
作者机构：
[Tang, Chao-ke; Li, Xiao-xu; Hu, Yan-wei; Liu, Xie-hong; Cao, Dong-li; Hao, Xin-rui; Xiao, Ji] Nanhua Univ, Univ S China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov,Life Sci Res Ctr, Hengyang 421001, Hunan, Peoples R China.;[Liao, Duan-fang] Univ S China, Inst Pharm & Pharmacol, Life Sci Res Ctr, Hengyang 421001, Hunan, Peoples R China.;[Xiang, Jim] Univ Saskatchewan, Dept Oncol, Res Unit, Hlth Res Div,Saskatchewan Canc Agcy, Saskatoon, SK S7N 4H4, Canada.
通讯机构：
[Tang, Chao-ke] N;Nanhua Univ, Univ S China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov,Life Sci Res Ctr, Hengyang 421001, Hunan, Peoples R China.
关键词：
ATP-binding cassette transporter A1;IFN-gamma;JAK/STAT1;Atherosclerosis;Reverse cholesterol transport
摘要：
Interferon gamma (IFN-gamma) is an immunomodulatory and anti-microbial cytokine, which has a variety of proatherogenic effects. It has been reported that IFN-gamma can down-regulate ABCA1 expression. However, its mechanism is elusive. In the present Study, we have investigated the effect of IFN-gamma on ABCA1 expression and cholesterol efflux in THP-1 macrophage-derived foam cells. IFN-gamma decreased ABCA1 expression at both transcriptional and translational levels in a dose-dependent manner. Cellular cholesterol content was increased while cholesterol efflux was decreased by IFN-gamma treatment. Liver X receptor a (LXR alpha), which can regulate the expression of ABCA1, was also down-regulated by IFN-gamma treatment. LXR alpha-specific activation by LXR alpha agonist almost compensated the down-regulation of ABCA1 expression by IFN-gamma, while siRNA of LXR alpha led to down-regulation of ABCA1 expression more significantly than IFN-gamma, IFN-gamma induced phosphorylation of STAT1 and expression of STAT1 alpha a in the nucleus, which was inhibited by a JAK inhibitor AG-490. Treatment with STAT1 siRNA further enhanced down-regulation of LXR alpha mRNA by IFN-gamma. Furthermore, AG-490 and STAT1 siRNA almost compensated the effect of IFN-gamma on ABCA1 expression and cholesterol efflux. In conclusion, IFN-gamma may first down-regulate expression of LXR alpha through the JAK/STAT1 signaling pathway and then decrease expression of ABCA1 and cholesterol efflux in THP-1 macrophage-derived foam cells. Therefore, our study may be useful in understanding the critical effect of IFN-gamma in pathogenesis of atherosclerosis. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
摘要：
Sox genes encode a family of transcription factors which are characterized by the conserved HMG domain and are involved in a diverse range of developmental processes. Using degenerate primer PCR, 7 different sequences encoding the HMG domains of Sox1, Sox9 and Sox1 1b were cloned and sequenced from genomic DNA in the red crucian carp (Carassius carassius red variety). In the case of Sox1 and Sox1 1b, we found evidence of gene duplication. In the phylogenetic tree, two paralogs of Sox1 1b (Sox1 1b1 and Sox1 1b2) fit within the clade of Sox11, especially in the Sox 1b subfamily, not in the Sox1 1a subfamily. Three Sox1 sequences (Soxl-1, Soxl-2 and Sox1-3) which shared the same amino acid sequence were identified in the red crucian carp. At the nucleotide level, Soxl-1 shared the highest sequence similarity to the zebrafish Sox1a, and Sox1-2 and Sox1-3 showed the highest similarity to the zebrafish Sox1b. The phylogenetic tree clearly demonstrated that the red crucian carp Sox1-1 clustered together with the zebrafish Sox1a, and the red crucian carp Sox1-2 and Sox1-3 with the zebrafish Sox1b. The result suggested that the red crucian carp Sox1-1, Soxl-2 and Sox1-3 were resulted from duplication of Sox1 gene rather than polymorphisms of the same gene.
作者：
Scaringe, William A.;Li, Kai;Gu, Dongqing;Gonzalez, Kelly D.;Chen, Zhenbin;...
期刊：
HUMAN MOLECULAR GENETICS,2008年17(18):2910-2918 ISSN：0964-6906
通讯作者：
Sommer, Steve S.
作者机构：
[Sommer, Steve S.] City Hope Natl Med Ctr, Beckman Res Inst, Dept Mol Genet, Duarte, CA 91010 USA.;[Li, Kai] Nanhua Univ, SNP Inst, Hengyang, Hunan, Peoples R China.;[Hill, Kathleen A.] Univ Western Ontario, Dept Biol, London, ON N6A 5B7, Canada.;[Sommer, Steve S.] City Hope Natl Med Ctr, Beckman Res Inst, Dept Mol Genet, 1500 E Duarte Rd, Duarte, CA 91010 USA.
通讯机构：
[Sommer, Steve S.] C;City Hope Natl Med Ctr, Beckman Res Inst, Dept Mol Genet, 1500 E Duarte Rd, Duarte, CA 91010 USA.
摘要：
Somatic microindels (microdeletions with microinsertions) have been studied in normal mouse tissues using the Big Blue lacI transgenic mutation detection system. Here we analyze microindels in human cancers using an endogenous and transcribed gene, the TP53 gene. Microindel frequency, the enhancement of 1–2 microindels and other features are generally similar to that observed in the non-transcribed lacI gene in normal mouse tissues. The current larger sample of somatic microindels reveals recurroids: mutations in which deletions are identical and the co-localized insertion is similar. The data reveal that the inserted sequences derive from nearby but not adjacent sequences in contrast to the slippage that characterizes the great majority of pure microinsertions. The microindel inserted sequences derive from a template on the sense or antisense strand with similar frequency. The estimated error rate of the insertion process of 13% per bp is by far the largest reported in vivo, with the possible exception of somatic hypermutation in the immunoglobulin gene. The data constrain possible mechanisms of microindels and raise the question of whether microindels are ‘scars’ from the bypass of large DNA adducts by a translesional polymerase, e.g. the ‘Tarzan model’ presented herein.
摘要：
1. Hydrogen sulphide (H(2)S) is a well-known cytotoxic gas. Recently, H(2)S has been shown to protect neurons against oxidative stress caused by glutamate, peroxynitrite and HOCl. Considerably lower H(2)S levels have been reported in the brain of Alzheimer's disease (AD) patients with accumulation of beta-amyloid (A beta). 2. The aim of present study was to explore the cytoprotection by H(2)S against A beta(25-35)-induced apoptosis and the molecular mechanisms underlying this effect in PC12 cells. 3. Our findings indicated that A beta(25-35) significantly reduced cell viability and induced apoptosis of PC12 cells, along with dissipation of the mitochondrial membrane potential (MMP) and overproduction of reactive oxygen species (ROS). 4. Sodium hydrosulphide (NaHS), an H(2)S donor, protected PC12 cells against A beta(25-35)-induced cytotoxicity and apoptosis not only by reducing the loss of MMP, but also by attenuating the increase in intracellular ROS. 5. The results of the present study suggest that the cytoprotection by H(2)S is related to the preservation of MMP and attenuation of A beta(25-35)-induced intracellular ROS generation. These findings could significantly advance therapeutic approaches to the neurodegenerative diseases that are associated with oxidative stress, such as AD.
作者机构：
[Xiao, L.; Li, Kai] Suzhou Univ, Mol Med Ctr, Suzhou 215006, Peoples R China.;[Xiao, L.; Li, Kai] Suzhou Univ, Second Affilated Hosp, Suzhou 215006, Peoples R China.;[Xiao, L.; Zhang, J.; Chen, C. L.; Li, Kai] Nanhua Univ, SNP Inst, Hengyang, Hunan, Peoples R China.;[Zhang, J.] Novartis Res Fdn, Genom Inst, San Diego, CA USA.;[Yin, Y. F.] City Hope Natl Med Ctr, Duarte, CA 91010 USA.
通讯机构：
[Zhang, I ] ;Nanhua Univ, SNP Inst, Hengyang, Hunan, Peoples R China.
摘要：
Alginate based microparticle drug delivery systems were prepared for the sustained release of antineoplastic drugs. Two drugs, 5-fluorouracil (5-FU) and tegafur, were encapsulated into the microparticles. The drug loaded microparticles were fabricated using a very convenient method under very mild conditions, i.e., directly shredding the drug loaded beads into microparticles in a commercial food processor. The mean sizes of the obtained microparticles were between 100 and 200 mu m. To effectively sustain the drug release, alginate microparticles were reinforced by chitosan during gelation. The drug release from the chitosan-reinforced alginate microparticles was obviously slower than that from the unreinforced microparticles. The effect of the reinforcement conditions on the drug release property of the microparticles was studied, and the optimized concentration of chitosan solution for reinforcement was identified. The effects of drug feeding concentration and pH value of the release medium on the drug release were investigated. (C) 2008 Elsevier B.V. All rights reserved.
作者机构：
[Liang, Lei; Zhu, Bing-Yang; Tuo, Qin-Hui; Liao, Duan-Fang; Cao, Xuan] Univ S China, Sch Life Sci & Technol, Div Pharmacoproteom, Hengyang 421001, Hunan Prov, Peoples R China.;[Tuo, Qin-Hui; Liao, Duan-Fang] Cent S Univ, Sch Pharmaceut Sci, Dept Pharmacol, Changsha 410078, Hunan Prov, Peoples R China.;[Liao, Duan-Fang] Univ S China, Sch Life Sci & Technol, Div Pharmacoproteom, Western Changsheng Rd 28, Hengyang 421001, Hunan Prov, Peoples R China.
通讯机构：
[Liao, Duan-Fang] U;Univ S China, Sch Life Sci & Technol, Div Pharmacoproteom, Western Changsheng Rd 28, Hengyang 421001, Hunan Prov, Peoples R China.
关键词：
Caveolin-1;Cholesterol;Daxx;Hepatic cells;Sterol regulatory element-binding protein
摘要：
AIM: To study the effect of Daxx on cholesterol accumulation in hepatic cells. METHODS: Sprague Dawley (SD) rats were fed a normal or high fat diet for 6 wk, and serum lipids and Daxx expression of hepatic tissues were measured by immunoblot assays. HepG(2) cells were transfected with the pEGFP-C1/Daxx or pEGFP-C1 plasmid. Cells stably transfected with Daxx were identified by RTPCR analysis. Total cholesterol levels were determined by high performance liquid chromatography. Activated-SREBP and caveolin-1 were assayed by western blotting. RESULTS: Hepatic Daxx protein was higher in normal rats than in high fat diet-fed rats. Noticeable negative correlations were seen between Daxx and LDL-C (gamma -7.56, P = 0.018), and between Daxx and TC (gamma -9.07, P = 0.01), respectively. The total cholesterol of HepG(2)/GFP-Daxx cells was lower than that of control cells or HepG(2)/GFP cells (9.28 +/- 0.19 vs 14.36 +/- 4.45 or 13.94 +/- 2.62, both P < 0.05). Furthermore, in HepG2/GFP cells, the expression of activated SREBP was lower than that of control cells, whereas caveolin-1 expression was higher. CONCLUSION: Overexpression of Daxx in HepG2 cells decreased intracellular cholesterol accumulation, which might be associated with inhibition of SREBP activity and an increase in caveolin-1 expression. (c) 2008 WJG. All rights reserved.
