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Isolation and expression of a novel alligator gene belonging to the Sox gene family

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成果类型:
期刊论文
作者:
Zheng, Jifang*;Hu, Nan;Zhu, Muyuan;Nu, Yaqing;Liu, Zhen
通讯作者:
Zheng, Jifang
作者机构:
[Zheng, Jifang; Hu, Nan; Nu, Yaqing; Liu, Zhen] Nanhua Univ, Coll Life Sci & Technol, Inst Genet, Hengyang 421001, Peoples R China.
[Zhu, Muyuan] Zhejiang Univ, Coll Life Sci, Inst Genet, Hangzhou 310012, Zhejiang, Peoples R China.
通讯机构:
[Zheng, Jifang] N
Nanhua Univ, Coll Life Sci & Technol, Inst Genet, Hengyang 421001, Peoples R China.
语种:
英文
关键词:
Alligator (Alligator sinensis);SRY;Sox gene;Cloning;Expression
关键词(中文):
鼍属和鳄属;氨基酸序列;动物;碱基序列;分子序列数据;序列排列
期刊:
Biochemical Genetics
ISSN:
0006-2928
年:
2009
卷:
47
期:
1-2
页码:
137-146
基金类别:
Acknowledgments We would like to acknowledge the staff of the Research Center for Chinese Alligator Reproduction in China for providing the experimental material, and the staff of Shanghai Biolink Informatics Inc. for expert technical assistance. This work was supported by the Natural Science Foundation of Hunan Province (Grant No. 04JJ40021) and the Scientific Research Foundation for Doctor in Nanhua University (Grant No. 5-03-XJQ-03-044).
机构署名:
本校为第一且通讯机构
院系归属:
药学与生物科学学院
摘要:
Sox genes share a highly conserved DNA-binding motif, the HMG (high mobility group)-box domain, and have diverse roles in vertebrate embryonic development. A novel SRY-related cDNA (temporarily called Sox33) isolated from the Chinese alligator (Alligator sinensis) is 1,819 bp in length, with an open reading frame from 220 to 1113 bp, encoding a protein of 298 amino acids. Two putative polyadenylation signal sequences (AATAAA) are present upstream of the poly(A) tail in the 3' UTR (at 1255-1260 and 1774-1779). The putative protein contains an HMG-box domain most closely related to hSox12, mSox4...

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