作者机构：
[Yang, Haibing; Fu, Xiaolei; Luo, Xin; Wang, Chengming] Department of Cardiovascular Medicine Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University Zhuzhou Hunan China;[Chen, Zhu] Hengyang Medical School, University of South China Hengyang Hunan China;[Chen, Fengling; Liu, Zhi; Chen, Yajie; Xu, Ting; Guo, Yuan] Department of Cardiovascular Medicine Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University Zhuzhou Hunan China<&wdkj&>Hengyang Medical School, University of South China Hengyang Hunan China
通讯机构：
[Xin Luo; Chengming Wang; Yuan Guo] D;Department of Cardiovascular Medicine Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University Zhuzhou Hunan China<&wdkj&>Department of Cardiovascular Medicine Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University Zhuzhou Hunan China<&wdkj&>Hengyang Medical School, University of South China Hengyang Hunan China
摘要：
Heart failure represents the terminal stage of cardiovascular disease, with cardiac remodeling as a key pathological feature. As a core circadian clock gene, brain and muscle aryl hydrocarbon receptor nuclear translocator-like 1 (BMAL1)not only regulates circadian rhythms but also plays a critical role in cardiac remodeling. Studies have shown that BMAL1 deficiency leads to myocardial metabolic dysfunction, cardiomyocyte hypertrophy, activation of the inflammatory response, fibrosis, and impaired cardiac function, thereby promoting the onset and progression of heart failure. This review systematically summarizes the mechanisms by which BMAL1 influences heart failure, with a focus on its regulatory roles in metabolism, inflammation, and fibrosis. Furthermore, we explore clinical translational potential and future prospects of correcting circadian rhythm disruption and chronotherapy in heart failure, aiming to provide new insights and directions for its treatment.
通讯机构：
[Yang, G ] C;Cent South Univ, Affiliated Childrens Hosp, Hunan Childrens Hosp, Dept Pediat Orthoped,Xiangya Sch Med, 86 Ziyuan Rd, Changsha 410007, Hunan, Peoples R China.
关键词：
long non-coding RNA;osteosarcoma;prognostic model;tumor immune infiltration
摘要：
Osteosarcoma (OS) is the most frequent type of cancer, as well as a common malignant tumor in orthopedics. Multiple long non-coding RNAs (lncRNAs) are implicated in immune infiltration in numerous types of cancer. Using single-sample gene set enrichment analysis to assign patients with OS, two immunotypes of OS were identified. Using K-means, spectral and PCA-K-means clustering two immunotypes of OS were demonstrated to be reproducible and represent a biologically meaningful classification of patients with OS based on their tumor immune microenvironment. Cluster 1 was an immune-infiltrating type, while Cluster 2 was an immune 'desert' type (low immune cell infiltration). Between Cluster 1 and 2, 29 common differentially expressed lncRNAs (DELs) were identified and univariate Cox regression (UCR) analysis was performed to identify DELs associated with overall survival. A risk score model was established by performing UCR and the iterative Lasso Cox regression analyses based on two immune infiltration-related lncRNA (IIRLs; LINC01094 and RP11-15K2.2). The risk model was a novel independent prognostic factor for patients with OS. A mutual influence between the expression of two IIRLs and mRNAs (LINC01094 and RP11-15K2.2) was also identified. Gene Ontology demonstrated that these mRNAs were abundant in immune-associated functions and pathways. Cytoscape was used to construct lncRNA-mRNA networks. The discrepancy in tumor-infiltrating immune cell abundance demonstrated that the high-risk group was associated with infiltration of diverse immune cell types. Furthermore, the expression levels of immune checkpoint inhibitors were markedly upregulated in the high- compared with that in the low-risk-cohort. Gene set enrichment analysis revealed that the risk score was associated with nucleotide oligomerization domain-like receptor signaling pathways. In summary, two IIRLs were developed in the present study to predict prognosis in patients with OS. The molecular mechanisms in the high-risk group may influence immune infiltration-linked biological processes.
期刊：
Chemical Communications,2025年61(85):16498-16511 ISSN：1359-7345
通讯作者：
Xing, H
作者机构：
[Huang, Xiaowei; Lin, Yingwu] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Xie, Wenxue; Huang, Xiaowei; Du, Jiajun; Li, Haokun; Xing, Hang] Hunan Univ, Inst Chem Biol & Nanomed, State Key Lab Chemo & Biosensing, Coll Chem & Chem Engn,Hunan Prov Key Lab Biomacrom, Changsha 410082, Peoples R China.;[Liu, Yuanyuan] Hunan Inst Drug Control, Changsha 410001, Hunan, Peoples R China.;[Xing, Hang] Hunan Univ Chongqing, Res Inst, Chongqing 401100, Peoples R China.
通讯机构：
[Xing, H ] H;Hunan Univ, Inst Chem Biol & Nanomed, State Key Lab Chemo & Biosensing, Coll Chem & Chem Engn,Hunan Prov Key Lab Biomacrom, Changsha 410082, Peoples R China.;Hunan Univ Chongqing, Res Inst, Chongqing 401100, Peoples R China.
摘要：
This highlight review article summarizes recent advances in employing HUH endonucleases as self-labeling protein tags for the sequence-specific covalent conjugation of unmodified ssDNA and examines their applications in cellular studies via engineered DNA-protein conjugates. We outline the structural basis and catalytic mechanism of the conserved HUH and Y motifs, which enable high selectivity, bioorthogonality, and robust conjugation under physiological conditions. Recent applications demonstrate the versatility of HUH-based DNA-protein conjugates in programmable cellular interface engineering, targeted therapeutic delivery, and enhancement of genome editing systems such as CRISPR-Cas. In the perspective section, we further highlight two emerging directions: computational tools such as the HUHgle platform for predictive substrate design, and directed evolution strategies extending HUH reactivity toward RNA substrates. Together, these advancements establish HUH endonucleases as powerful, programmable tools for generating DNA-protein conjugates that enable innovations in chemical biology, synthetic biology, and therapeutics.
作者机构：
School of Public Health, University of South China, Hengyang, 421001, China;The First Affiliated Hospital of University of South China, Hengyang, 421001, China;[Wu; Rang; Zhao; Zhang; Yao] 南华大学公共卫生学院;[Xiong] 南华大学公共卫生学院<&wdkj&>南华大学附属第一医院
摘要：
Persistent and maladaptive drug-related memories represent a key component in drug addiction. Converging evidence from both preclinical and clinical studies has demonstrated the potential efficacy of the memory reconsolidation updating procedure (MRUP), a non-pharmacological strategy intertwining two distinct memory processes: reconsolidation and extinction-alternatively termed "the memory retrieval-extinction procedure". This procedure presents a promising approach to attenuate, if not erase, entrenched drug memories and prevent relapse. The present review delineates the applications, molecular underpinnings, and operational boundaries of MRUP in the context of various forms of substance dependence. Furthermore, we critically examine the methodological limitations of MRUP, postulating potential refinement to optimize its therapeutic efficacy. In addition, we also look at the potential integration of MRUP and neurostimulation treatments in the domain of substance addiction. Overall, existing studies underscore the significant potential of MRUP, suggesting that interventions predicated on it could herald a promising avenue to enhance clinical outcomes in substance addiction therapy.
摘要：
Background: The non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio (NHHR) is a recently developed composite indicator of atherogenic lipids. Nevertheless, few studies concern the relationship between NHHR and early-onset post-stroke depression (PSD). Methods: After two weeks of acute ischemic stroke (AIS), early-onset PSD was identified. The Hamilton Depression Scale-17 items (HAMD-17) was used to assess the severity of depression. Patients with HAMD-17 scores ≥7 were divided into an early-onset PSD group. Spearman rank correlation analysis was employed to evaluate the associations between NHHR and HAMD scores across all patients. Logistic regression analysis was conducted to investigate the associations between the NHHR and early-onset PSD. Sensitivity analyses were performed to test the robustness of our findings. Receiver operating characteristic curve (ROC) analysis was used to determine the predictive value of the NHHR for early-onset PSD. Results: Among the 846 patients who were enrolled prospectively, a total of 283 (33.45%) patients were diagnosed with early-onset PSD. The NHHR showed a positive correlation with the HAMD-17 scores (r=0.498, P<0.001). A binary logistic regression model demonstrated that the NHHR (odds ratio [OR], 1.796; 95% confidence interval [CI] 1.452-1.996, P<0.001) was an independent factor for early-onset PSD. The NHHR for early-onset PSD had an area under the curve (AUC) value of 0.798. Conclusions: The findings suggest that the NHHR may be an independent risk factor for early-onset PSD, providing valuable insights for prevention and prognostic management in affected patients.
期刊：
Particulate Science and Technology,2025年43(4):637-650 ISSN：0272-6351
通讯作者：
Deng, R
作者机构：
[Deng, Rong; Luo, Yufeng; Deng, R] Univ South China, Sch Mech Engn, Hengyang 421000, Hunan, Peoples R China.;[Li, Sizhong] Hunan Hergelix intelligent Technol Co LTD, Shachang, Hunan, Peoples R China.
通讯机构：
[Deng, R ] U;Univ South China, Sch Mech Engn, Hengyang 421000, Hunan, Peoples R China.
关键词：
Fresh concrete;DEM;B m;shape and content of coarse aggregate;specific surface area
摘要：
In this paper, the effect of the coarse aggregate (CA) shape and content on the flow performance index of fresh concrete in the L-box test (B m ) was investigated based on Discrete Element Method (DEM). The Hertz-Mindlin-JKR (Johnson-Kendall-Roberts) contact model was used to characterize the constitutive relationships of fresh concrete. The parameters of contact model were calibrated by the experiment and simulation of the slump test, L-box flow test and V-funnel test. The shape of CA was quantitatively characterized, and a functional relationship between the specific surface area of concrete (S C ) and surface energy was established. The effect of CA shape and content on B m was studied numerically. The simulated results of single-shape CA concrete in L-box test show that the shape of CA affects the resultant force and flow velocity of the tracked concrete in the x direction, thus affecting the B m . The smaller the sphericity index of CA, the larger the specific surface area and the smaller the B m . The results of orthogonal tests show that the CA content of different shape in concrete affects B m with different degree. The larger the specific surface area of coarse aggregate (S CA ), the greater the negative effect on B m . A linear relationship between S C and B m was established. An equation for predicting B m based on shapes and contents of CA was presented. It shows that the more complex the shape of CA and the higher its content, the smaller the B m .
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2025年73(26):16011–16027 ISSN：0021-8561
通讯作者：
Bo Su
作者机构：
[Xinyi Guo; Bo Su] Institute of Pharmacy and Pharmacology, School of Pharmacy, Hengyang Medical School, University of South China, Chang Sheng Xi Avenue 28, Hengyang, Hunan 421001, China
通讯机构：
[Bo Su] I;Institute of Pharmacy and Pharmacology, School of Pharmacy, Hengyang Medical School, University of South China, Chang Sheng Xi Avenue 28, Hengyang, Hunan 421001, China
摘要：
Diallyl trisulfide (DATS), an organosulfur compound from the common food garlic, has been attracting attention by exhibiting low toxicity, tumor susceptibility, and, especially, multiple antitumor activities, despite its simple molecular structure. The mounting data suggest that the effects of DATS are related to its induction of oxidative stress cell death in various forms via reactive oxygen species (ROS), such as apoptosis, ferroptosis, autophagy, and pyroptosis. Disruption of redox equilibrium by DATS involves not only the induction of ROS generation but also the inhibition of ROS scavenging through modulation of antioxidant systems, which in turn affects the redox-dependent key molecules in the signaling pathways, consequently curbing the malignant behavior of cancers. Retracing the progress of DATS antitumor research in the past decade, we conduct an in-depth analysis of the multitarget mechanisms of DATS intervening in redox homeostasis. This review provides clues for future studies to further reveal its potential antitumor effects.
摘要：
Mycoplasma represents a unique genus of prokaryotic bacteria distinguished by the absence of a cell wall, a characteristic that sets it apart from other bacteria. Within the Mollicutes class, phylogenetic analysis reveals three distinct categories: Spiroplasma, Mycoplasma and Acholeplasma. Mycoplasmas within Pneumoniae are recognized for their capacity to induce a range of diseases in both humans and animals, frequently impacting respiratory and reproductive health. The representative strains in Pneumoniae group, particularly the M. pneumoniae clusters, have garnered significant attention due to their remarkable ability to adhere to, invade, and traverse host cells. This ability is facilitated by specialized structures known as attachment organelles, which possess a unique cytoskeletal structure that supports a distinctive gliding motility mechanism. This mode of motility is distinct from that observed in eukaryotes and the majority of bacteria. The gliding machinery of Mycoplasma is a complex assembly consisting of both surface and internal components, including a terminal button, paired plates, and a structure resembling a bowl or wheel. The internal architecture of the attachment organelles provides the essential scaffold for the operation of this sophisticated motility system. Mycoplasma's gliding motility is crucial for its infection process and its capacity to evade the host immune defenses. Understanding the role of this motility to immune evasion can offer profound insights into the pathogenesis of these bacteria, could pave the way for the development of more effective therapeutic strategies against diseases caused by Mycoplasma and related species.
摘要：
Acute kidney injury (AKI) is associated with poor prognosis. New biomarkers, like neutrophil gelatinase-associated lipocalin (NGAL), are helpful for early warning of AKI. This study aims to investigate the accuracy of NGAL in evaluating the perioperative AKI of liver transplantation. The four databases, PubMed, Web of Science, Embase, and Cochrane Library, were searched for relevant studies published from database inception to August 2023. Results were pooled using random-effects models, and heterogeneity was examined. A total of 16 case-control studies with 1271 patients were included. The results showed that both preoperative [standardized mean difference (SMD) = 0.53; 95% confidence interval (CI): 0.15, 0.91; P < 0.001] and postoperative NGAL levels (SMD = 0.63; 95% CI: 0.24, 1.03; P < 0.001) were higher in the AKI group compared with the non-AKI group. Subgroup analysis by continents showed higher preoperative NGAL levels in AKI patients in the European population (SMD = 1.63; 95% CI: 0.55, 0.27; P = 0.003), but no differences in Asian, African, North American, and South American. Subgroup analysis by continents revealed higher postoperative NGAL levels in the European (SMD = 1.63; 95% CI: 0.55, 0.27; P = 0.002) and Asian populations (SMD = 0.42; 95% CI: 0.04, 0.81; P = 0.039). Higher postoperative NGAL levels in plasma and urine were observed in AKI patients compared with non-AKI patients [plasma (SMD = 1.29; 95% CI: 0.21, 2.38; P = 0.011), urine (SMD = 0.88; 95% CI: 0.18, 1.59; P = 0.035)], while there was no difference in African, North American, South American, and serum NGAL. NGAL level may be an important biomarker for early detection of AKI in the perioperative period of liver transplantation.
