基础化学课程建设改革探索
作者：
廖力夫;肖锡林;林英武;聂长明
期刊：
当代教育理论与实践 ,2015年(4):52-54 ISSN：1674-5884
作者机构：
南华大学 化学化工学院,湖南 衡阳,421001;[聂长明; 林英武; 肖锡林; 廖力夫] 南华大学
关键词：
地方高校;基础化学;教学改革
摘要：
基础化学是医学生的一门重要基础课程。以地方高校基础化学教学特点和长期教学实践为基础,为适应高等教育大众化和创新型人才培养的要求,从地方高校基础化学课程教学内容、课堂教学及实践教学等环节的改革和创新入手,突出医学教育的特点,培养医学生从化学角度理解医学问题的能力,为地方培养高素质医学人才。
语种：
中文
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湘江衡阳段镉污染分析及环境风险评价
作者：
肖锡林;薛金花;程健琳;尹纪成;廖力夫;...
期刊：
应用化工 ,2015年44(2):214-216 ISSN：1671-3206
作者机构：
[肖锡林; 薛金花; 程健琳; 尹纪成; 廖力夫; 周斌; 何博] 南华大学化学化工学院公共卫生学院
关键词：
湘江衡阳段;镉污染;石墨炉原子吸收法;环境风险评价
摘要：
用石墨炉原子吸收法检测了湘江衡阳市蒸湘区段和常宁市水口山矿区段各60名男性人体尿样和两个地区不同环境水体的镉含量, 进行环境风险评价。结果表明, 衡阳市蒸湘区60名男性人体尿样中Cd的平均浓度为2. 74 ng /mL, 水中Cd的平均浓度为0. 42 ng /mL;水口山矿区60名男性矿工尿样中Cd的平均浓度为40. 26 ng /mL;河水中Cd的平均浓度为7. 02 ng /mL。水口山矿区水样中Cd的浓度和60名男性矿工尿样中Cd的浓度都高于衡阳市蒸湘区水样和居民尿样中Cd的浓度。这表明, 在重金属矿区由于环境的影响, 无论是环境水中还是职业接触者体内, 镉的含量都高于一般地区和普通人群。
语种：
中文
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Resonance light scattering for detecting fluoride ions based on the formation of a uranyl coordination supramolecular polymer
作者：
Shen, Xing;Liao, Lifu* ;Chen, Lin;Xu, Canhui;He, Yunfei;...
期刊：
ANALYTICAL METHODS ,2014年6(13):4818-4822 ISSN：1759-9660
通讯作者：
Liao, Lifu
作者机构：
[Xu, Canhui; Chen, Lin; Xiao, Xilin; Liao, Lifu; Nie, Changming; Lin, Yingwu; Shen, Xing; He, Yunfei] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, Lifu] U;Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
摘要：
A resonance light scattering (RLS) method for the detection of fluoride ions in aqueous solution is reported in this paper. Uranyl-bis-salophen (UBS), a binuclear uranyl complex prepared by the reaction of uranyl with a ditopic tetradentate Schiff base ligand bis-salophen, was used for the experiment. It is found that after UBS self-assembled with pyrophosphate to form a uranyl coordination supramolecular polymer (SP), the system produced a strong RLS signal. When fluoride ions coexisted with UBS, the formation of SP was inhibited due to which the fluoride ions occupied the coordination sites in UBS. This resulted in RLS quenching. Based on these findings, we established a RLS method for the detection of fluoride ions in aqueous solution. Under optimal conditions, the linear range was found to be 0.005 to 1.5 nmol mL-1 with a detection limit of 0.0028 nmol mL-1. The method was applied to determine fluoride ions in environmental water samples with relative standard deviations of 1.3 to 3.1% and recoveries of 98.7-101.3%. © the Partner Organisations 2014.
语种：
英文
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水中痕量镉的曙红Y-中性红能量转移荧光猝灭法测定
作者：
肖锡林;薛金花;廖力夫;肖明春;杜可杰;...
期刊：
南华大学学报(自然科学版) ,2014年(1):91-96 ISSN：1673-0062
作者机构：
南华大学 化学化工学院,湖南 衡阳,421001;南华大学 公共卫生学院,湖南 衡阳,421001;[肖明春; 薛金花; 邓昌爱; 何博; 杜可杰; 肖锡林; 廖力夫] 南华大学
关键词：
曙红Y;中性红;镉离子;能量转移荧光猝灭法
摘要：
在pH 7.9的BR缓冲液中,曙红Y-中性红和镉离子相互作用,在535 nm处荧光强度随着镉浓度的增加,体系的荧光逐渐降低,其荧光强度降低值和镉离子含量在一定范围内有相关关系.据此建立了一种测定痕量镉的曙红Y-中性红体系能量转移荧光猝灭新方法,并优化了实验条件.在优化的实验条件下,体系在535 nm处的荧光强度降低值与镉的摩尔浓度在1.6＆#215;10-8~3.2＆#215;10-6 mol/L范围内线性关系良好,其线性回归方程为：？F=936.60-2425.2c（＆#215;10-6mol/L）,相关系数r=0.998,检出限为4.7＆#215;10-9 mol/L,相对标准偏差是0.45%,加标回收率是90.75%.本方法操作简便、灵敏、选择性好,用于实际样品中镉的测定,其测定结果同石墨炉原子吸收法一致（t检验法）.
语种：
中文
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基于磺基Salophen配位反应荧光法测定铀酰
作者：
陈琳;沈杏;何云飞;徐灿辉;廖力夫
期刊：
应用化工 ,2014年43(5):936-938 ISSN：1671-3206
作者机构：
[陈琳; 沈杏; 何云飞; 徐灿辉; 廖力夫] 南华大学化学化工学院
关键词：
铀酰;磺基Salophen;荧光;配位反应
摘要：
用5-磺基水杨醛与邻苯二胺反应，合成了水溶性良好的四齿西佛碱配体磺基 Salophen。研究了磺基 Salo-phen 与铀酰离子发生配位反应生成配合物铀酰-磺基 Salophen 过程的荧光光谱。结果表明，磺基 Salophen 与铀酰配位后体系的荧光显著增强，最大发射波长位于428 nm。应用该反应建立了荧光分析法测定水溶液中铀酰离子的新方法。在优化条件下测定铀酰的线性范围为0.03~4.0 nmol/ mL，检出限为0.015 nmol/ mL。应用建立的荧光法测定了实际水样中的铀酰，回收率为97.0％~104.0％。
语种：
中文
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Detection of Mycoplasma genitalium Using a Quantum dot Sensitization Wireless Magnetoelastic Immunosensor
作者：
Bo He;Xilin Xiao;Yanhua Zeng;Lifu Liao;Yimou Wu;...
作者机构：
[Bo He; Xilin Xiao; Lifu Liao; Shuqin Gao] College of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China;[Yanhua Zeng; Yimou Wu] Institute of Pathogenic Bio1ogy, Medical College, University of South China, Hengyang 421001, China
会议名称：
The 6th Meeting of the Asian Organization for Mycoplasmology (AOM)The 7th Meeting of the Chinese Society for Mycoplasmology (CSM)(第七届全国支原体学术会议暨第六届亚洲支原体学术会议)
会议时间：
2014-8-22
会议地点：
张家界
会议主办单位：
中华医学会微生物与免疫学分会;亚洲支原体学组织
会议论文集名称：
The 6th Meeting of the Asian Organization for Mycoplasmology (AOM)The 7th Meeting of the Chinese Society for Mycoplasmology (CSM)(第七届全国支原体学术会议暨第六届亚洲支原体学术会议)论文集
关键词：
Mycoplasma genitalium;Wireless;Quantum dot
摘要：
A wireless immunosensor for the detection of Mycoplasma genitalium was fabricated by immobilizing polyclonal antibody onto the surface of a magnetostrictive strip.In response to a time-varying magnetic field, the immunosensor longitudinally vibrates at a resonance frequency, emitting magnetic flux that can be remotely detected by a pickup coil.
语种：
英文
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水中痕量铜的中性红－曙红Y同步荧光猝灭法检测
作者：
肖锡林;薛金花;廖力夫;贺冬秀;王芳;...
期刊：
分析仪器 ,2014年(6):24-29 ISSN：1001-232X
作者机构：
南华大学化学化工学院，南华大学公共卫生学院，衡阳 421001;江西中医学院现代中药制剂教育部重点实验室,南昌,330004;[薛金花; 何博; 王芳; 肖锡林; 贺冬秀; 廖力夫] 南华大学;[袁金斌] 江西中医学院
关键词：
曙红Y;中性红;铜;同步荧光猝灭
摘要：
在pH8.5的T ris－HCl缓冲液中，曙红Y －中性红和Cu（Ⅱ）相互作用，在538nm处随着Cu（Ⅱ）浓度的增加，体系同步荧光强度逐渐降低，同步荧光强度降低值和Cu（Ⅱ）含量在一定范围内有相关关系。据此应用中性红－曙红Y同步荧光猝灭法建立一种测定铜的新方法。在优化的实验条件下，体系在538nm处的荧光强度降低值与铜的摩尔浓度在7.61＆#215;10－8－3.60＆#215;10－6 mol ＆#183; L －1范围内线性关系良好，其线性回归方程为：△ F＝－0.414＋314.89c（＆#215;10－6 mol ＆#183; L －1），相关系数 r＝0.998，检出限为2.48＆#215;10－8 mol ＆#183; L －1，相对标准偏差是0.26％，加标回收率是101.12％。本方法操作简便、灵敏、选择性好，可用于实际样品中铜的测定。
语种：
中文
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A resonance light scattering method for the determination of uranium based on a water-soluble salophen and oxalate
作者：
Chen, Lin;Liao, Lifu* ;Shen, Xing;He, Yunfei;Xu, Canhui;...
期刊：
Journal of Radioanalytical and Nuclear Chemistry ,2014年301(3):863-869 ISSN：0236-5731
通讯作者：
Liao, Lifu
作者机构：
[Xu, Canhui; Chen, Lin; Xiao, Xilin; Liao, Lifu; Nie, Changming; Lin, Yingwu; Shen, Xing; He, Yunfei] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, Lifu] U;Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
会议名称：
Biennial Symposium on Nuclear and Radiochemistry
会议时间：
FEB 19-23, 2013
会议地点：
Jabalpur, INDIA
会议主办单位：
[Chen, Lin;Liao, Lifu;Shen, Xing;He, Yunfei;Xu, Canhui;Xiao, Xilin;Lin, Yingwu;Nie, Changming] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Uranium;Resonance light scattering;Water-soluble salophen;Oxalate
摘要：
A resonance light scattering (RLS) method for the direct detection of uranium (VI) or uranyl in aqueous solution without separation procedure has been reported in this paper. Sulfo-salophen, a water-soluble tetradentate Schiff base ligand of uranyl, reacted with uranyl to form a complex. The complex reacted further with oxalate to form supramolecular dimer with large molecular volume, resulting in a production of strong RLS signal. The amount of uranium (VI) was detected through measuring the RLS intensity. A linear range was found to be 0.2-30.0 ng/mL under optimal conditions with a detection limit of 0.15 ng/mL. The method has been applied to determine uranium (VI) in environmental water samples with the relative standard deviations of less than 5 % and the recoveries of 98.8-105.8 %. The present technique is suitable for the assay of uranium (VI) in environmental water samples collected from different sources. © 2014 Akadémiai Kiadó.
语种：
英文
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基于卓越工程师培养的“分析化学”教学改革探讨
作者：
廖力夫;肖锡林;王宏青;聂长明;林英武;...
期刊：
中国电力教育 ,2014年(23):86-87 ISSN：1007-0079
作者机构：
南华大学化学化工学院,湖南 衡阳,421001;[刘文娟; 聂长明; 王宏青; 林英武; 肖锡林; 王平; 廖力夫] 南华大学
关键词：
地方高校;分析化学;小组讨论式教学;双语教学;卓越工程师
摘要：
“分析化学”是化学工程与工艺等专业的必修学科基础课程，在工科人才培养中起着重要作用。目前中外教育交流日益频繁，教育国际化趋势明显，如何合理借鉴海外先进教学经验，是一个值得探索的课题。以所在地方高校为例，坚持以学生为中心，进行“分析化学”小组讨论式双语教学模式改革，将课内与课外、科学与人文、教学与研究结合起来，形成独具特色的卓越工程师人才培养模式，满足时代发展的需求，为实现国际化人才培养目标打下良好基础。
语种：
中文
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地方高校分析化学实验教学改革与实践
作者：
肖锡林;廖力夫;王宏青;聂长明;刘文娟
期刊：
大学教育 ,2014年(16):128-129 ISSN：2095-3437
作者机构：
南华大学 化学化工学院,湖南 衡阳,421001;[刘文娟; 聂长明; 王宏青; 肖锡林; 廖力夫] 南华大学
关键词：
地方高校;分析化学实验;教学改革
摘要：
为提升毕业生的培养质量，针对不同“核”专业的具体特点而进行分析化学实验教学改革是一个良好的途径和有效措施。应针对本科分析化学实验教学存在的不足之处，开展全方位的教学改革。改革以紧密结合学生、学校和专业特点为主要特征，对现有的分析化学实验教学内容、教学方法等相关方面进行改革实践，建立新的开放式实验教学新模式，为培养建设社会主义的高级专门人才打下良好的基础。
语种：
中文
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水中痕量镉(Ⅱ)的荧光素-曙红Y能量转移荧光猝灭测定法
作者：
肖锡林;薛金花;廖力夫;赵睿;周斌;...
期刊：
环境与健康杂志 ,2014年31(6):525-527 ISSN：1001-5914
作者机构：
南华大学化学化工学院,湖南衡阳421001;[薛金花; 赵睿; 周斌] 南华大学公共卫生学院;[肖锡林; 廖力夫; 何博] 南华大学化学化工学院
关键词：
荧光素;曙红Y;能量转移;荧光猝灭法;镉(Ⅱ)
摘要：
目的建立水中痕量镉(Ⅱ)的荧光素-曙红Y能量转移荧光猝灭测定法。方法取0.50ml的水样置于5.0 ml的比色管中,分别加入1×10~(-4) mol/L荧光素溶液0.30 ml,1×10~(-5) mol/L曙红Y溶液0.10 ml,BR缓冲液(pH 6.5)0.50 ml,定容后在502 nm处测定溶液荧光强度(F)值,以内标法定量。结果在3.43×10~(-7) ~1.246×10~(-5) mol/L的线性范围为,该方法所得镉(Ⅱ)的线性回归方程为ΔF=14.221+546.81×10~(-5)c,r=0.999 5。该方法的检出限为1.03×10~(-8) mol/L,平均回收率为94.53%~98.15%,RSD为2.44%~4.67%。结论该方法操作简便、灵敏、选择性好,适用于水中镉(Ⅱ)的测定。
语种：
中文
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Detection of Mycoplasma Genitalium by using a Wireless Magnetoelastic Biosensor
作者：
Xilin Xiao;Bo He;Yanhua Zeng;Lifu Liao;Yimou Wu;...
作者机构：
[Xilin Xiao; Bo He; Lifu Liao; Shuqin Gao] College of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China;[Yanhua Zeng; Yimou Wu; Youcong Zhu] Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, China
会议名称：
The 6th Meeting of the Asian Organization for Mycoplasmology (AOM)The 7th Meeting of the Chinese Society for Mycoplasmology (CSM)(第七届全国支原体学术会议暨第六届亚洲支原体学术会议)
会议时间：
2014-8-22
会议地点：
张家界
会议主办单位：
中华医学会微生物与免疫学分会;亚洲支原体学组织
会议论文集名称：
The 6th Meeting of the Asian Organization for Mycoplasmology (AOM)The 7th Meeting of the Chinese Society for Mycoplasmology (CSM)(第七届全国支原体学术会议暨第六届亚洲支原体学术会议)论文集
关键词：
Magnetoelastic biosensor;Detection;Mycoplasma genitalium
摘要：
Magnetoelastic biosensors are wireless passive sensors and develop rapidly in recent years.Magnetoelastic materials are made of amorphous ferromagnetic alloys composed of iron, nickel,molybdenum and boron.
语种：
英文
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地方高校研究生课程双语教学实践与探索
作者：
肖锡林;邓健;廖力夫;赵玉宝;袁亚莉;...
期刊：
化工高等教育 ,2014年31(1):27-30 ISSN：1000-6168
作者机构：
南华大学化学化工学院,湖南衡阳,421001;[聂长明; 王宏青; 袁亚莉; 邓健; 赵玉宝; 林英武; 肖锡林; 廖力夫] 南华大学
关键词：
地方高校;研究生;双语教学;小组讨论式教学;化学与生物传感器
摘要：
随着中外教育交流的日益频繁和教育国际化趋势的逐渐增强,研究生英语教学之外的专业英语交流及应用越来越受到人们的重视。研究生是否具备专业英语素养和专业英语应用能力已成为评判复合型研究生人才的一项重要指标。素质教育背景下的研究生双语教学体现了时代发展的需求,是实现国际化人才培养目标的有效途径。小组讨论式教学法是目前世界上许多国家普遍采用的一种富有创意的教学理论与方法。本文试以所在地方高校研究生课程化学与生物传感器教学实践为例,结合小组讨论式教学法,探索地方高校研究生双语教学改革的思路与实践模式。
语种：
中文
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Spectroscopic study on the reactions of bis-salophen with uranyl and then with fructose 1,6-bisphosphate and the analytical application
作者：
Shen, Xing;Liao, Lifu* ;Chen, Lin;He, Yunfei;Xu, Canhui;...
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2014年123:110-116 ISSN：1386-1425
通讯作者：
Liao, Lifu
作者机构：
[Xu, Canhui; Chen, Lin; Xiao, Xilin; Liao, Lifu; Nie, Changming; Lin, Yingwu; Shen, Xing; He, Yunfei] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, Lifu] U;Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Bis-salophen;Fluorescence;Fructose 1,6-bisphosphate;Resonance light scattering;Uranyl
摘要：
The chelating reaction of bis-salophen with uranyl to form binuclear complex uranyl-bis-salophen (UBS) was studied by fluorescence spectroscopy. The coordination reaction of UBS with fructose 1,6-bisphosphate (F-1,6-BP) to form supramolecular polymer was then studied by resonance light scattering (RLS) spectroscopy. The reaction of bis-salophen with uranyl results in a remarkable enhancement of fluorescence intensity. The maximum emission wavelength of the fluorescence is at 471 nm. The reaction of UBS with F-1,6-BP results in a remarkable enhancement of RLS intensity. The maximum scattering wavelength of the RLS is at 460 nm. The two reactions were used to establish fluorescence method for the determination of uranium (VI) and RLS method for the determination of F-1,6-BP, respectively. Under optimum conditions, the linear ranges for the detection of uranium (VI) and F-1,6-BP are 0.003-0.35 nmol/mL and 0.05-5.0 nmol/mL, respectively. The detection limits are 0.0017 nmol/mL and 0.020 nmol/mL, respectively. The proposed fluorescence method has been successfully applied for the determination of uranium (VI) in environmental water samples with the recoveries of 97.0-104.0%. The proposed RLS method has also been successfully applied for the determination of F-1,6-BP in medicine injection samples with the recoveries of 98.5-102.3%. ©2013 Elsevier B.V. All rights reserved.
语种：
英文
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Determination of trace metallothioneins at nanomolar levels using phenanthroline-copper coordination by fluorescence spectra
作者：
Xiao, Xilin* ;Xue, Jinhua;Liao, Lifu;Chen, Xiangcheng;Zeng, Yanhua;...
期刊：
Analytical Sciences ,2014年30(10):999-1004 ISSN：0910-6340
通讯作者：
Xiao, Xilin
作者机构：
[Chen, Xiangcheng; Wu, Yimou; Xiao, Xilin; Liao, Lifu; Xue, Jinhua; Zeng, Yanhua] Univ South China, Coll Chem & Chem Engn, Coll Publ Hlth, Inst Pathogen Biol, Hengyang City 421001, Hunan, Peoples R China.
通讯机构：
[Xiao, Xilin] U;Univ South China, Coll Chem & Chem Engn, Coll Publ Hlth, Inst Pathogen Biol, Hengyang City 421001, Hunan, Peoples R China.
关键词：
Metallothioneins;copper;1,10-phenanthroline;fluorescence
摘要：
A direct fluorescence spectra method was applied for the determination of metallothioneins at nanomolar levels. In Britton-Robison (B-R) buffer (pH 7.0), the interaction of bis(1,10-phenanthroline)copper(II) complex cation [Cu(phen)2]2+ and metallothioneins enhanced the fluorescence intensity of system. The fluorescence enhancement at 365 nm was proportional to the concentration of metallothioneins. The mechanism was studied and discussed in terms of the fluorescence and UV-absorption spectra. Under the optimal experimental conditions, at 365 nm, there was a linear relationship between the fluorescence intensity and the concentration of the metallothioneins in the range of 8.30 × 10-9 - 7.70 × 10-7 mol L-1. The linear regression equation was ΔF = 8.96 + 38.01c (mol L-1), with a correlation coefficient of r = 0.998 and detection limit 2.50 × 10-9 mol L-1. The relative standard deviation was 0.47% (n = 11), and the average recovery 97.2%. The proposed method was successfully reliable, selective and sensitive in determining trace metallothioneins in fish visceral organ samples with the results in good agreement with those obtained by HPLC. © The Japan Society for Analytical Chemistry.
语种：
英文
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基于信息技术与化学学科有效整合的创新人才培养研究
作者：
聂长明;廖力夫;林英武;肖新荣;邓健;...
期刊：
中国电力教育 ,2013年(23):20-21 ISSN：1007-0079
作者机构：
南华大学化学化工学院 湖南 衡阳 421001;[聂长明; 邓健; 肖新荣; 肖锡林; 林英武; 廖力夫; 周昕] 南华大学
关键词：
信息技术;化学学科;有效整合;创新人才
摘要：
信息技术是人们利用信息器官功能的各种方法、工具与技能的总和。基于化学学科的特点和实际,对信息技术与化学学科有效整合的教学平台设计、创新意识、创新思维培养及整合实践效果进行了系统探讨和分析。
语种：
中文
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Determination of fructose 1,6-bisphosphate using a double-receptor sandwich type fluorescence sensing method based on uranyl–salophen complexes
作者：
Zhang, Guangliang;Liao, Lifu* ;Lin, Yingwu;Yang, Miao;Xiao, Xilin;...
期刊：
Analytica Chimica Acta ,2013年784:47-52 ISSN：0003-2670
通讯作者：
Liao, Lifu
作者机构：
[Yang, Miao; Xiao, Xilin; Zhang, Guangliang; Liao, Lifu; Nie, Changming; Lin, Yingwu] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, Lifu] U;Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Double-receptor;Fluorescence;Fructose 1,6-bisphosphate;Salophen;Sandwich;Uranyl
摘要：
In this paper, we report a double-receptor sandwich type fluorescence sensing method for the determination of fructose bisphosphates (FBPs) using fructose 1,6-bisphosphate (F-1,6-BP) as a model analyte based on uranyl-salophen complexes. The solid phase receptor is an immobilized uranyl-salophen (IUS) complex which is bound on the surface of glass slides by covalent bonds. The labeled receptor is another uranyl-salophen complex containing a fluorescence group, or uranyl-salophen-fluorescein (USF). In the procedure of determining F-1,6-BP in sample solution, F-1,6-BP is first adsorbed on the surface of the glass slide through the coordination reaction of F-1,6-BP with IUS. It then binds USF through another coordination reaction to form a sandwich-type structure of IUS-F-1,6-BP-USF. The amount of F-1,6-BP is detected by the determination of the fluorescence intensity of IUS-F-1,6-BP-USF bound on the glass slide. Under optimal conditions, the linear range for the detection of F-1,6-BP is 0.05-5.0nmolmL-1 with a detection limit of 0.027nmolmL-1. The proposed method has been successfully applied for the determination of F-1,6-BP in real samples with satisfactory results. © 2013 Elsevier B.V.
语种：
英文
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Determination of ATP using a double-receptor sandwich method based on molecularly imprinted membrane and fluorescence-labeled uranyl-salophen complex
作者：
Yang, Miao;Liao, Lifu* ;Zhang, Guangliang;Xiao, Xilin;Lin, Yingwu;...
期刊：
Analytical and Bioanalytical Chemistry ,2013年405(23):7545-7551 ISSN：1618-2642
通讯作者：
Liao, Lifu
作者机构：
[Yang, Miao; Xiao, Xilin; Liao, Lifu; Zhang, Guangliang; Nie, Changming; Lin, Yingwu] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, Lifu] U;Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
ATP;Double-receptor;Fluorescence;Molecularly imprinted membrane;Sandwich;Uranyl-salophen complex
摘要：
A double-receptor sandwich method for the fluorescence determination of adenosine triphosphate (ATP) is proposed in this paper. The solid phase receptor on the surface of glass slides is a molecularly imprinted membrane (MIM) containing an artificial nanocavity. It is constructed by a molecular imprinting technique using adenosine monophosphate (AMP) as a template molecule. The labeled receptor is a uranyl-salophen complex containing a fluorescent group or uranyl-salophen-fluorescein (USF). It is synthesized with salophen, 5-aminofluorescein, and uranyl. In a procedure of determining ATP, ATP in sample solution is first adsorbed on the surface of the glass slide through the combination of the AMP group in ATP with the nanocavity in MIM. Then, the adsorbed ATP binds USF through the coordination reaction of the phosphate group in ATP with uranyl in USF to form a sandwich-type structure of MIM-ATP-USF. The amount of ATP is detected through the fluorescence determination of USF bound on the slide. Under optimal conditions, the linear range for the determination of ATP is 0.3 to 4.8 nmol/mL with a detection limit of 0.041 nmol/mL. The proposed method has been successfully employed for the determination of ATP in real samples with the recoveries of 98.5 to 102.5 %. © 2013 Springer-Verlag Berlin Heidelberg.
语种：
英文
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Simultaneous determination of copper, lead and zinc in alloy by potentiometric titration coupled with multivariate calibration using a flow injection technique
作者：
Deng, Changai* ;Liao, Lifu;Long, Wei
期刊：
Advanced Materials Research ,2013年785-786:1353-1358 ISSN：1022-6680
通讯作者：
Deng, Changai
作者机构：
[Long, Wei; Liao, Lifu; Deng, Changai] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Deng, Changai] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
会议名称：
3rd International Conference on Chemical Engineering and Advanced Materials (CEAM 2013)
会议时间：
JUL 06-07, 2013
会议地点：
Guangzhou, PEOPLES R CHINA
会议主办单位：
[Deng, Changai;Liao, Lifu;Long, Wei] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
会议论文集名称：
Advanced Materials Research
关键词：
Alloy;Complexometric titration;Flow injection technique;Multivariate calibration
摘要：
A new potentiometric titration method coupled with multivariate calibration using a flow injection technique for the simultaneous determination of multi-components in alloy has been developed. the titrant is a mixture of EDTA and sodium fluoride, both mercury film electrode and fluorinion selective electrode are inserted in the flow cell. In a process of titration, both the potentiometric of mercury ion and the mixed proportion of reactants at each titration point can be obtained simultaneously from the two electrodes, and then the concentration of each component in the mixture can be obtained from the titration curves by multivariate calibration method. The method is free of volumetric and time measurements, and reduces the consumption of reagents and samples. The method is also very quick in analysis rapidity. The method has been applied for the simultaneous determination of copper, lead and zinc in alloy samples with the relative standard deviation of 0.18%~0.45% and the recovery of 99.5%~101.6%.
语种：
英文
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磷酸化聚乙烯醇修饰磁性纳米粒子对铀酰的吸附性能研究
作者：
杨淼;张光亮;沈杏;陈琳;廖力夫
期刊：
广东化工 ,2013年40(4):5-6+134 ISSN：1007-1865
作者机构：
南华大学化学化工学院,湖南衡阳,421001;[陈琳; 张光亮; 杨淼; 廖力夫; 沈杏] 南华大学
关键词：
铀酰;吸附;磁性纳米粒子;磷酸化聚乙烯醇
摘要：
在Fe3O4磁性纳米粒子表面复盖一层磷酸化聚乙烯醇制备了一种用于从水相选择性分离铀酰的复合型磁性纳米粒子.用平衡吸附法研究了纳米粒子对铀酰的吸附性能和选择识别能力,且探讨了实验条件对纳米粒子吸附性能的影响.结果表明在竞争金属离子存在下,纳米粒子对铀酰具有较高的吸附能力和选择识别能力,且可以循环使用.
语种：
中文
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