摘要：
A kind of functional plant composite adsorbent was prepared through diethylenetriamine modifing the Cycas revoluta leaves for low concentration of U(VI) in waste water. The dynamic behavior of the adsorption, the Langmuir and Freundlich equations and the thermodynamic parameters was were also examined in the present study. The experimental results showed that diethylenetriamine functionalized Cycas revoluta leaves used for low concentration of uranium possessed many advantages such as widely raw material sources, simple and economy for preparation, less adsorbent dosage, short balance time, high adsorption efficiency, good chemical and mechanical stability, and can be effective regeneration or reused.
会议论文集名称：
International Conference on Materials, Environmental and Biological Engineering, Part 1: Guilin(CN).28-30 March 2015
关键词：
Staphylococcus aureus ssp. anaerobius (S. aureus);Penicillin G;Targeted drug delivery;Bone;Ti-Al intermetallic compounds porous material
摘要：
Bacterial infection is one of the most common problems after orthopedic implant surgery. If not prevented, bacterial infection can result in serious and life threatening conditions such as osteomyelitis. Thus, in order to reduce chances of such serious complication, patients are often subjected to antibiotic drug therapy for 6-8 weeks after initial surgery. The antibiotics are systemically delivered either intravenously, intramuscularly or topically. Systemic antibiotic delivery entails certain drawbacks such as systemic toxicity and limited bioavailability. Further, in order for the drug to be effective at the site of implantation, high doses are required, which can result in undesired side effects in patients. Thus, local antibiotic therapy is the preferred way of administering drugs. To that end, we have developed Ti-Al intermetallic compounds porous material for local delivery of antibiotics off-implant at the site of implantation. The Ti-Al intermetallic compounds porous materials were fabricated with reactively synthesizing. The fabrication strategies allow us to precisely control the dimension of aperture, thus enabling us to load different amounts of drugs and control the release rates. In this work we have fabricated Ti-Al intermetallic compounds porous material with 40 similar to 200 nm and 2 similar to 5 mu m apertures. We have loaded these tubes with 180, 360 and 540 mu g of penicillin G. The penicillin G release kinetics from the apertures and its effect on Staphylococcus aureus ssp. anaerobius (S. aureus) adhesion were investigated. Further, an osteosarcoma cell line called MG-63 was cultured on penicillin G-loaded aperture to evaluate the effect of Ti-Al intermetallic compounds porous material on cell functionality. Our results indicate that we can effectively fill the apertures with the drug and the drug eluting apertures significantly reduced bacterial adhesion on the surface. Also, there is enhanced MG-63 differentiation on apertures filled with penicillin G.
摘要：
A Schiff base-type fluorescence probe was prepared for the detection of copper (II) in foodstuffs. The probe is N,N'-bis(pyridoxal phosphate)-o-phenylenediamine (BPPP). It was synthesized by utilizing the Schiff base condensation reaction of pyridoxal 5-phosphate with 1,2-phenylenediamine. BPPP has the properties of high fluorescence stability, good water solubility and low toxicity. Its maximum excitation wavelength and maximum fluorescence emission wavelength are at 389 and 448 nm, respectively. When BPPP coexists with copper (II), its fluorescence is dramatically quenched. Under a certain condition, the fluorescence intensity decreased proportionally to the concentration of copper (II) by the quenching effect. Based on this fact, we established a fluorescence quenching method for the determination of copper (II). Under optimal conditions a linear range was found to be 0.5-50 ng/mL with a detection limit of 0.2 ng/mL. The method has been applied to determine copper (II) in foodstuff samples and the analytical results show good agreement with that obtained from atomic absorption spectrometry method. (C) 2015 Elsevier B.V. All rights reserved.
摘要：
We describe a resonance light scattering (RLS) method for the direct detection of adenosine triphosphate (ATP) in aqueous solution without the need for separation. The binuclear uranyl complex bis-uranyl-bis-sulfosalophen (BUBSS) was synthesized by the coordination reaction of two uranyl ions with the ditopic tetradentate ligand bis-sulfosalophen. In parallel, gold nanoparticles were modified with anti-ATP aptamer (Apt-AuNPs) to serve as RLS probes. The detection scheme is based on the specific reaction of a BUBSS molecule with two ATP molecules to form a linear supramolecule. This, in turn, binds two Apt-AuNPs molecules, and this is accompanied by the aggregation of the Apt-AuNPs and results in a strong RLS signal. A RLS method was developed on this basis that has a linear response to ATP in the 5 to 500 nM concentration range, with a 4.5 nM detection limit under optimal conditions. The method has been successfully applied to the detection of ATP in spiked real samples and gave recoveries between 96.3 and 102.5 %. Graphical Abstract We describe a resonance light scattering (RLS) method for the direct detection of adenosine triphosphate (ATP) in aqueous. Gold nanoparticles were modified with anti-ATP aptamer (Apt-AuNPs) to serve as RLS probes. The detection scheme is based on the specific reaction of binuclear uranyl complex molecule with two ATP molecules to form a linear supramolecule.
摘要：
The fabrication of tiron-copper complex as a novel fluorescence probe for the sensitive directly detection of metallothioneins at nanomolar levels was demonstrated. In Britton-Robinson (B-R) buffer (pH 7.50), the interaction of bis(firon)copper(II) complex cation [Cu(tiron)(2)](2+) and metallothioneins enhanced the fluorescence intensity of the system. The fluorescence enhancement at 347 nm was proportional to the concentration of metallothioneins. The mechanism was studied and discussed in terms of the fluorescence spectra. Under the optimal experimental conditions, at 347 nm, there was a linear relationship between the fluorescence intensity and the concentration of the metallothioneins in the range of 8.80 x 10(-9)-7.70 x 10(-7) mol L-1, with a correlation coefficient of r = 0.995 and detection limit 2.60 x 10(-9) mol L-1. The relative standard deviation was 0.77% (n = 11), and the average recovery 94.4%. The method proposed was successfully reliable, selective and sensitive in determining of trace metallothioneins in fish visceral organ samples with the results in good agreement with those obtained by HPLC. (C) 2015 Elsevier B.V. All rights reserved.
