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Determination of trace metallothioneins at nanogram levels with Eosin Y by resonance light scattering method

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成果类型:
期刊论文
作者:
Xiao, Xilin*;Xue, Jinhua;Ding, Dexin;Liao, Lifu;Meng, Xialing;...
通讯作者:
Xiao, Xilin
作者机构:
[Ding, Dexin; Zhou, Bin; Meng, Xialing; Liu, Liangzhuan; Xiao, Xilin; Liao, Lifu; Xue, Jinhua; He, Bo] Univ South China, Key Discipline Lab Natl Def Biotechnol Uranium Mi, Coll Chem & Chem Engn, Coll Publ Hlth,Inst Pathogen Biol, Hengyang City 421001, Peoples R China.
通讯机构:
[Xiao, Xilin] U
Univ South China, Key Discipline Lab Natl Def Biotechnol Uranium Mi, Coll Chem & Chem Engn, Coll Publ Hlth,Inst Pathogen Biol, Hengyang City 421001, Peoples R China.
语种:
英文
关键词:
Eosin Y;interactions;absorption spectra;resonance light scattering (RLS);metallothioneins (MTs)
期刊:
International Journal of Environmental Analytical Chemistry
ISSN:
0306-7319
年:
2015
卷:
95
期:
6
页码:
520-530
基金类别:
Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31200140, 11475079]; Defense Industrial Technology Development Program [B3720132001]; construct program of Innovation Team in USC [NHCXTD15]
机构署名:
本校为第一且通讯机构
院系归属:
化学化工学院
核资源工程学院
医学院
公共卫生学院
摘要:
The interactions of metallothioneins with Eosin Y were studied by the absorption spectra and resonance light scattering (RLS) spectra methods. A direct RLS spectra method was applied for the determination of trace metallothioneins. The interaction of Eosin Y and metallothioneins enhanced the RLS intensity of system in Britton-Robinson buffer (pH 3.91), with the help of anionic surfactant (sodium dodecyl benzene sulphate). The mechanism was studied and discussed in terms of the RLS and UV-absorption spectra. Under optimal experimental conditions, at 366 nm, there was a linear relationship ...

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