作者：
Liu, Ai Ling*;Liao, Hong Qing;Li, Zhi Liang;Liu, Jun;Zhou, Cui Lan;...
期刊：
Asian Pacific Journal of Cancer Prevention,2016年17(12):5087-5094 ISSN：1513-7368
通讯作者：
Liu, Ai Ling
作者机构：
[Xie, Hong Yan; Li, Zhi Liang; Peng, Cui Ying; Liu, Ai Ling; Liu, Jun] Institute of Biological Science, The Key Laboratory of Biological Toxicology and Ecological Restoration of Hengyang City, School of Pharmaceutical and Biological Science, Heng Yang, Hunan Province, 421001, China;[Liao, Hong Qing] Reproductive Health Hospital of Nanhua-Xinghui in Hengyang City, Heng Yang, Hunan Province, 421001, China;[Zhou, Cui Lan] Department of Anatomy, University of South China, Heng Yang, Hunan Province, 421001, China;[Guo, Zi Fen] Institute of Pharmacy and Pharmacology, University of South China, Heng Yang, Hunan Province, 421001, China
通讯机构：
[Liu, Ai Ling] I;Institute of Biological Science, The Key Laboratory of Biological Toxicology and Ecological Restoration of Hengyang City, School of Pharmaceutical and Biological Science, University of South China, Heng yang 421001, Hunan Province, China. Email:
关键词：
mTOR signaling pathway;PCOS;ovarian cancer
摘要：
mTOR, the mammalian target of rapamycin, is a conserved serine/threonine kinase which belongs to the phosphatidyl-linositol kinase-related kinase (PIKK) family. It has two complexes called mTORC1 and mTORC2. It is well established that mTOR plays important roles in cell growth, proliferation and differentiation. Over-activation of the mTOR pathway is considered to have a relationship with the development of many types of diseases, including polycystic ovary syndrome (PCOS) and ovarian cancer (OC). mTOR pathway inhibitors, such as rapamycin and its derivatives, can directly or indirectly treat or relieve the symptoms of patients suffering from PCOS or OC. Moreover, mTOR inhibitors in combination with other chemical-molecular agents may have extraordinary efficacy. This paper will discuss links between mTOR signaling and PCOS and OC, and explore the mechanisms of mTOR inhibitors in treating these two diseases, with conclusions regarding the most effective therapeutic approaches.
摘要：
Doxorubicin (DOX) is a potent and available antitumor therapeutic agent; however, its clinical application is limited due to its cardiotoxicity. Preliminary evidence suggests that hydrogen sulfide (H2S) may exert protective effects on DOX-induced cardiotoxicity. Therefore, the aim of the present study was to investigate whether the extracellular signal-regulated kinase (ERK) 1/2 signaling pathway is involved in the cardioprotection of H2S against DOX-induced cardiotoxicity. The present study demonstrated that pretreatment with sodium hydrosulfide (NaHS; a donor of H2S) prior to DOX exposure attenuated the decreased cell viability, the increased apoptosis rate and the intracellular accumulation of reactive oxygen species (ROS) in H9c2 cardiac myocytes. Exposure of H9c2 cardiac myocytes to DOX upregulated the expression levels of phosphorylated ERK1/2, which had been reduced by pretreatment with NaHS or N-acetyl-L-cysteine, a ROS scavenger. In addition, H2S upregulated the anti-apoptotic protein, Bcl-2 and downregulated the pro-apoptotic protein, Bax. Notably, U0126, a selective inhibitor of ERK1/2, was observed to mimic the above-mentioned cytoprotective activity of H2S. In conclusion, these findings indicate that H(2)5 attenuates DOX-induced cardiotoxicity by inhibiting ROS-mediated activation of ERK1/2 in H9c2 cardiac myocytes.
摘要：
Finite-difference time-domain (FDTD) method is employed to study the optical properties of a novel kind of periodic subwavelength hole arrays composed of Tai-chi-shaped holes in silver film, and the optical transmission properties of femtosecond optical pulse excitation is numerically calculated. We find that this Tai-chi-shaped device has better optical band-pass filtering properties, such as narrower pass band and higher transmissivity in visible wavelengths range, than other devices under consideration. Based on the generation of surface plasmons resonance mode in the dielectric-metal interface, the center wavelength of transmission can be tuned by changing the array periodicities. We observe that the tune ability mainly depends on the space period along the direction parallel to that of the incident pulse polarization. It is also found that both the strength and the wavelength of the transmission peaks of rectangularly distributed metal hole arrays are determined by the polarization of incident light. Additionally, we demonstrate the typical band-pass filtering properties of this Tai-Chi-shaped holes structure. The full-width at half-maximum (FWHM) of the narrow pass band is about 20 nm in visible wavelengths range. (C) 2014 Elsevier B.V. All rights reserved.
摘要：
Background: The posterior interosseous artery (PIA) perforator flap can be used for reconstruction of soft-tissue defects of fingers. Based on the multiple perforators from the posterior interosseous artery, we describe a technique to reconstruct the multi-finger defect in the use of the free multilobed PIA perforator flap. Methods: PIA perforators from different areas of the forearm were used to design a free multilobed skin paddle for multi-finger skin defect reconstruction. Each paddle without the deep fascia had separate perforators. To increase the perforator pedicle length, the courses of the PIA perforators were dissected from the superficial layer of the deep fascia to the subcutaneous layer. Results: The flap was raised as a unilateral free bilobed PIA perforator flap in 10 cases of two-finger defects, a free trilobed PIA perforator flap in two cases of three-finger defects, and a bilateral free bilobed PIA perforator flap in one case of four-finger defects. The average effective vascular pedicle length and trunk pedicle length were 8.3 and 3.1 cm, respectively, for the bilobed flap, and 6.3 and 4.0 cm, respectively, for the trilobed flap. All flaps survived except one paddle with tip necrosis. At 10.8 months (range, 4-27 months) after surgery, 10 cases showed satisfactory cosmetic appearance, while the fingers were bulky in the remaining three cases. The average score of static two-point discrimination in 10 innervated paddles was 12.9 mm. The remaining 20 paddles recovered only protective sensation. The average total active motion (TAM) of each finger was 164 degrees before surgery and 187 degrees at the latest follow-up. Conclusions: Free multilobed PIA perforator flap is a good candidate for reconstruction of multi-finger skin defect. Clinical question/level of evidence: Therapeutic, IV. (C) 2014 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.
摘要：
The present study aimed to investigate the effect of hydrogen sulfide (H2S) on kidney injury induced by urinary-derived sepsis. Rabbits were randomly divided into control, sham, sepsis, NaHS 2.8 mumol/kg and NaHS 8.4 mumol/kg groups, with six rabbits in each group. Upper urinary tract obstruction and acute infection was induced to establish the sepsis model. Blood was collected to carry out a white blood cell (WBC) count, and creatinine (Cr) and blood urea nitrogen (BUN) analysis. Morphological changes were observed by hematoxylin and eosin (H&E) staining and transmission electron microscopy. Immunohistochemical staining was used to detect the expression levels of tumor necrosis factor (TNF)-alpha, interleukin (IL)-10 and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB). Cystathionine-gamma-lyase (CSE) activity was measured by the spectrophotometric methylene blue method and the blood H2S concentration was measured by deproteinization. WBC, Cr and BUN levels were significantly elevated in the sepsis group compared with those in the control group (P<0.05). Following treatment with NaHS, the WBC, Cr and BUN levels were significantly decreased in the NaHS groups compared with those in the sepsis group (P<0.05). The pathological features of kidney injury were also alleviated by NaHS. In the sepsis group, the levels of TNF-alpha, IL-10 and NF-kappaB were significantly increased compared with those in the control group (P<0.05). In the NaHS groups, the TNF-alpha and NF-kappaB levels were significantly reduced whereas the IL-10 level was significantly increased compared with the respective levels in the sepsis group (P<0.05). The H2S concentration was significantly decreased in the sepsis group and this reduction was attenuated in the NaHS groups (P<0.05). Furthermore, the NaHS 8.4 mumol/kg dose revealed a more potent effect than the NaHS 2.8 mumol/kg dose. Thus, exogenous H2S reduced kidney injury from urinary-derived sepsis by decreasing the levels of NF-kappaB and TNF-alpha, and increasing the level of IL-10.
