赛尔能源三矿A4007工作面瓦斯涌出规律与治理
作者：
赵勇;罗才武;雷波;刘军
期刊：
南华大学学报(自然科学版) ,2017年31(3):34-38 ISSN：1673-0062
作者机构：
南华大学环境与安全工程学院;[刘军] 中煤科工集团重庆研究院有限公司;[罗才武; 赵勇; 雷波] 南华大学
关键词：
涌出量;瓦斯抽采;埋管抽放;预抽;边采边抽
摘要：
为降低赛尔能源三矿 A 4 0 0 7工作面瓦斯含量, 本文计算了巷道煤壁瓦斯涌出量、 落煤瓦斯涌出量, 以及开采层相对瓦斯涌出量, 并分析了工作面瓦斯涌出规律.得出回采工作面相对瓦斯涌出量为1. 5 4m3/ t , 绝对瓦斯涌出量为3. 1 1m3/m i n, 占涌出总量的1 5. 6%, 工作面瓦斯主要来源于采空区. 针对性的提出工作面采用采空区埋/插管抽放, 老空区封闭插管抽放, 本煤层预抽、 边采边抽、 强化抽放, 上隅角密闭抽放. 对抽采效果进行检验, 治理后上隅角瓦斯浓度基本控制在1%以下, 其平均瓦斯浓度为0. 6 6 8%, 极小值为0. 3%, 极大值为0. 8 4%. 回风流瓦斯浓度基本控制在0. 4%以下, 平均瓦斯浓度为0. 2 6 4%, 极小值为0. 1 2%, 极大值为0. 3 8%.
语种：
中文
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A wave-dominated heat transport mechanism for negative differential thermal resistance in graphene/hexagonal boron nitride heterostructures
作者：
Chen, Xue-Kun* ;Liu, Jun;Peng, Zhi-Hua;Du, Dan;Chen, Ke-Qiu*
期刊：
Applied Physics Letters ,2017年110(9):091907 ISSN：0003-6951
通讯作者：
Chen, Xue-Kun;Chen, Ke-Qiu
作者机构：
[Du, Dan; Peng, Zhi-Hua; Chen, Xue-Kun] Univ South China, Sch Math & Phys, Hengyang 421001, Peoples R China.;[Liu, Jun] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Chen, Ke-Qiu] Hunan Univ, Sch Phys & Elect, Dept Appl Phys, Changsha 410082, Hunan, Peoples R China.;[Chen, Ke-Qiu] Synerget Innovat Ctr Quantum Effects & Applicat H, Changsha 410081, Hunan, Peoples R China.
通讯机构：
[Chen, Xue-Kun] U;[Chen, Ke-Qiu] H;[Chen, Ke-Qiu] S;Univ South China, Sch Math & Phys, Hengyang 421001, Peoples R China.;Hunan Univ, Sch Phys & Elect, Dept Appl Phys, Changsha 410082, Hunan, Peoples R China.
关键词：
boron compounds;graphene;molecular dynamics method;semiconductor superlattices;thermal resistance;vibrational modes
摘要：
Nonlinear thermal transport in graphene/hexagonal boron nitride (h-BN) heterostructure is investigated by the nonequilibrium molecular dynamics method. It is found that negative differential thermal resistance (NDTR) will appear as the applied temperature difference increases. Detailed phonon spectra analysis reveals that the excited out-of-plane acoustic wave plays an important role in the heat transport across such interface. That is, the mechanical wave results in a significant mismatch between the lattice vibrations of graphene and h-BN domains and hinders interfacial thermal transport. In addition, NDTR can be tuned through the temperature parameter. Interestingly, the regime of NDTR becomes smaller and eventually vanishes with increasing the heterostructure length. However, NDTR is insensitive to the variation of system width. The work may be useful for nanoscale thermal managements utilizing the graphene/h-BN heterostructure. © 2017 Author(s).
语种：
英文
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肌酸作为运动营养补充剂的副作用与安全性
作者：
刘军
期刊：
科技经济导刊 ,2017年(21):187 ISSN：2096-1995
作者机构：
南华大学体育部;[刘军] 南华大学
关键词：
肌酸;副作用;安全性;疗效
摘要：
肌酸作为目前最有效的营养补充剂,它通过加速运动员体内磷酸肌酸合成发挥强身健体功效。我国多数运动员在训练过程中都使用肌酸,但关于肌酸的副作用及安全性还知之甚少。研究发现,健康成年人（除孕妇及哺乳期妇女外）每天摄取3g肌酸不会对身体产生不良影响。但长期大量摄入肌酸能引起胃肠道疾病、肌肉痉挛、体重增加等不良反应,并对肝、肾、心血管系统等产生不良影响,引发机体出现肌肉痉挛、脱水、头晕等副反应。本文主要综述肌酸作为营养补充剂的可能副作用与安全性,从而能更全面了解并合理使用肌酸。
语种：
中文
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Imbalance of Th1/Th2 and Th17/Treg promoting schistosome egg granuloma formation
作者：
Chen, Shanshan;Gao, Yongqiang;Liang, Yu;Hu, Li;Liu, Jun;...
期刊：
International Journal of Clinical and Experimental Medicine ,2017年10(10):14290-14300 ISSN：1940-5901
通讯作者：
Xiao, Jianhua
作者机构：
[Liang, Yu; Chen, Shanshan; Xiao, Jianhua; Peng, Li; Gao, Yongqiang; Feng, Angui; Hu, Li; Liu, Jun] Univ South China, Inst Pathogen Biol, Med Coll, Hengyang 421001, Peoples R China.;[Liang, Yu; Chen, Shanshan; Xiao, Jianhua; Peng, Li; Gao, Yongqiang; Feng, Angui; Hu, Li; Liu, Jun] Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang, Peoples R China.;[Xiao, Jianhua; Gao, Yongqiang; Hu, Li] Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang, Peoples R China.
通讯机构：
[Xiao, Jianhua] U;Univ South China, Inst Pathogen Biol, Med Coll, Hengyang 421001, Peoples R China.
关键词：
CD4(+) T cell;Th1/Th2;Th17/Treg;schistosoma japonicum;egg granuloma
摘要：
Objective: This research aimed to reveal the correlation between the liver pathology of schistosome egg granuloma and CD4+ T cell subsets through analyzing the dynamic changes of CD4+ T cell subset (Th1/Th2 and Th17/Treg) and their related cytokines. Methods: The BalB/c mice were infected with schistosoma japonicum cercariae to construct a model of schistosomiasis japonica. The blood samples and liver tissues were harvested at the 4th,6th, 8th, 10th, 12th, 16th, 20th and 24th week, respectively. Flow cytometry (FCM) was conducted to evaluate the proportion of CD4+ T cell subsets including Th1, Th2, Th17 and Treg. Enzyme-linked immunosorbent assay (ELISA) was performed to assess the serum level of cytokines including IL-2, IFN-γ, IL-4, IL-10, IL-17, IL-23, IL-6, TGF-β and IL- 1β; while the quantitative real-time PCR (qRT-PCR) was carried out to investigate the mRNA level of these cytokines in liver tissues. Finally, Haematoxylin and eosin (HE) staining and Masson staining were performed to assess the pathological characteristics of the liver. Results: Histopathological evaluation of liver in infected mice showed the dynamic changes of pathological features, such as inflammatory cell infiltration, schistosome egg granuloma formation, liquefactive necrosis of eggs and eventually a lot of cavities formation. Masson staining also demonstrated that the collagen deposition increased before the 12th week, ultimately developed into hepatic fibrosis from the 16th to 24th week. Both of Th1/Th2 and Th17/Treg ratios were distributed as a bimodal pattern with peaks at the 4th and 16th week. At the same time, the levels of CD4+ T cell related nuclear transcription factors and cytokines showed similar dynamic changes as the percentages of T cell subsets. Conclusion: The imbalance of Th1/Th2 and Th17/ Treg were closely related to the schistosome egg granuloma formation. The imbalance of CD4+ T cell subsets maybe play an important role in the schistosome egg granuloma formation. © 2017, E-Century Publishing Corporation. All rights reserved.
语种：
英文
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空气舵磨削加工手动夹紧夹具
发明/设计人：
唐德文;何彬;张家雨;彭聪;刘军
申请/专利权人：
南华大学
申请/专利号：
CN201710546750.2
申请时间：
2017-7-6
公开号：
CN107186623A
公开时间：
2017-9-22
主申请人地址：
421001 湖南省衡阳市蒸湘区常胜西路28号南华大学
摘要：
空气舵磨削加工手动夹紧夹具，包括底板、主板、中间板、锁止套筒、入口套、拉紧组件；主板竖直固接在底板上端；中间板平行于主板，并与主板相对固定，其上设有方孔；入口套安装在主板的安装孔中并与主板固接，其一端伸入到主板与中间板之间的区间内；锁止套筒位于主板与中间板之间的区间内，并通过其内孔中的光孔段活动套装在入口套的外壁上，其内孔与入口套的内孔连通并正对中间板的方孔；拉紧组件安装在中间板上并与锁止套筒关联，其可拉动锁止套筒向中间板移动，并使锁止套筒与中间板在彼此相对距离最近时保持相对固定。本发明只需一套夹具便可实现空气舵磨削加工时的装夹定位，解决了现有的空气舵磨削加工夹具成本偏高的问题。
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Anomalous thermal conductance of graphyne under lower temperature
作者：
Chen, Xue-Kun* ;Liu, Jun;Du, Dan;Chen, Ke-Qiu
期刊：
JOURNAL OF PHYSICS-CONDENSED MATTER ,2017年29(45):455702 (8pp) ISSN：0953-8984
通讯作者：
Chen, Xue-Kun
作者机构：
[Du, Dan; Chen, Xue-Kun] Univ South China, Sch Math & Phys, Hengyang 421001, Peoples R China.;[Liu, Jun] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Chen, Ke-Qiu] Hunan Univ, Dept Appl Phys, Sch Phys & Elect, Changsha 410082, Hunan, Peoples R China.;[Chen, Ke-Qiu] Synerget Innovat Ctr Quantum Effects & Applicat H, Changsha 410081, Hunan, Peoples R China.
通讯机构：
[Chen, Xue-Kun] U;Univ South China, Sch Math & Phys, Hengyang 421001, Peoples R China.
关键词：
graphyne;thermal conductance;molecular dynamics;coherent phonons
摘要：
The thermal transport properties of graphyne are investigated via equilibrium molecular dynamics (EMD) simulations and non-equilibrium Green's function (NEGF) method. It is found that the room-temperature thermal conductivity of graphyne is 93% lower than that of graphene with a similar size and decreases steeply with increasing the number of acetylenic linkages, which agrees with the results obtained by NEGF method qualitatively. Lattice dynamics calculations reveal that these phenomena can be attributed to the reduction of both phonon group velocities and phonon lifetimes in graphyne at low-frequency region. However, when the temperature is less than 30 K, the thermal conductance of graphyne exceeds that of graphene. Moreover, the anomalous thermal conductance behavior is not sensitive to the system lateral size. The underlying mechanisms for such phenomena are elaborated by the normal mode analysis.<br/> ©2017 IOP Publishing Ltd.
语种：
英文
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高校体育文化建设提升体育人文素养的路径探析
作者：
刘军
期刊：
长江丛刊 ,2017年(23):218-219 ISSN：2095-7483
作者机构：
南华大学体育学院;[刘军] 南华大学
关键词：
体育文化;人文素养;建设;提升
摘要：
高校体育文化对丰富校园文化,提升大学生的综合素质及文化素养具有积极影响。通过解读高校体育文化与体育人文素养内涵,分析了高校体育人文素养的要素主要体现方面,提出了高校体育文化提升人文素养的基本路径。
语种：
中文
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Remarkable reduction of thermal conductivity in graphyne nanotubes by local resonance
作者：
Chen, Xue-Kun;Chen, Chang-Yong;Liu, Jun;Chen, Ke-Qiu*
期刊：
Journal of Physics D: Applied Physics ,2017年50(34):345301 ISSN：0022-3727
通讯作者：
Chen, Ke-Qiu
作者机构：
[Chen, Xue-Kun; Chen, Ke-Qiu] Hunan Univ, Sch Phys & Elect, Dept Appl Phys, Changsha 410082, Hunan, Peoples R China.;[Chen, Xue-Kun] Univ South China, Sch Math & Phys, Hengyang 421001, Peoples R China.;[Chen, Chang-Yong] Shaoguan Univ, Dept Phys, Shaoguan 512005, Peoples R China.;[Liu, Jun] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Chen, Ke-Qiu] Synerget Innovat Ctr Quantum Effects & Applicat H, Changsha 410081, Hunan, Peoples R China.
通讯机构：
[Chen, Ke-Qiu] H;[Chen, Ke-Qiu] S;Hunan Univ, Sch Phys & Elect, Dept Appl Phys, Changsha 410082, Hunan, Peoples R China.;Synerget Innovat Ctr Quantum Effects & Applicat H, Changsha 410081, Hunan, Peoples R China.
关键词：
thermal conductivity;graphyne nanotubes;phonon lifetime
摘要：
By using equilibrium molecular dynamics simulations and lattice dynamic calculations, we investigate the thermal transport properties of graphyne nanotubes (GNTs). The results demonstrate that the GNTs exhibit relatively low thermal conductivity, which is approximately 10-25% of that in a carbon nanotube. Detailed lattice dynamic analyses indicate that the presence of sp carbon atoms can induce local resonance modes in the low frequency region, which results in the remarkable decrease of thermal conductivity. Meanwhile, the high-frequency phonon lifetimes also drop because of the vibrational mismatch between sp and sp<sup>2</sup>carbon atoms (enhanced phonon scattering). Our results show the local resonance of phonons is an important mechanism for the reduction of thermal conductivity in low-dimensional nanostructures. ©2017 IOP Publishing Ltd.
语种：
英文
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异形火箭舱段机加夹具
发明/设计人：
唐德文;何彬;彭聪;刘军;张家雨;...
申请/专利权人：
南华大学
申请/专利号：
CN201710604713.2
申请时间：
2017-7-24
公开号：
CN107159919A
公开时间：
2017-09-15
摘要：
异形火箭舱段机加夹具，包括基座、步进电机、回转支承、中间圆板、回转传动机构、端面接触台、径向夹紧装置及轴向压紧装置；回转传动机构设在步进电机与中间圆板之间，其将步进电机的动力传递至中间圆板；端面接触台固接在中间圆板的上端；径向夹紧装置有四组，分别安装在端面接触台的四个定位槽内；轴向压紧装置有四组，分别安装在四组径向夹紧装置的保护壳上。本发明以异形火箭舱段的内孔为基准，通过三组径向夹紧装置的三个卡爪实现快速自动定心，同时通过三组轴向压紧装置将工件压紧，再通过第一销锁定装置和第二销锁定装置实现工件端面上的销孔自动定位，从而达到快速装夹定位找正的目的。装夹过程自动化程度高，可靠性好。
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空气舵磨削加工手动夹紧夹具
发明/设计人：
何彬;唐德文;张家雨;彭聪;刘军
申请/专利权人：
南华大学
申请/专利号：
CN201720812950.3
申请时间：
2017-7-6
公开号：
CN207206167U
公开时间：
2018-4-10
主申请人地址：
421001 湖南省衡阳市蒸湘区常胜西路28号南华大学
摘要：
空气舵磨削加工手动夹紧夹具，包括底板、主板、中间板、锁止套筒、入口套、拉紧组件；主板竖直固接在底板上端；中间板平行于主板，并与主板相对固定，其上设有方孔；入口套安装在主板的安装孔中并与主板固接，其一端伸入到主板与中间板之间的区间内；锁止套筒位于主板与中间板之间的区间内，并通过其内孔中的光孔段活动套装在入口套的外壁上，其内孔与入口套的内孔连通并正对中间板的方孔；拉紧组件安装在中间板上并与锁止套筒关联，其可拉动锁止套筒向中间板移动，并使锁止套筒与中间板在彼此相对距离最近时保持相对固定。本实用新型只需一套夹具便可实现空气舵磨削加工时的装夹定位，解决了现有的空气舵磨削加工夹具成本偏高的问题。
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衡阳市本科院校公共体育课程教学现状的调查研究
作者：
刘军;黄诚
期刊：
湖南科技学院学报 ,2017年38(6):133-135 ISSN：1673-2219
作者机构：
南华大学 体育学院,湖南 衡阳,421001;[刘军; 黄诚] 南华大学
关键词：
衡阳;本科院校;公共体育;体育教学;现状;调查研究
摘要：
通过对衡阳市本科院校公共体育课程教学现状进行调查,针对学校的教学模式,场地器材及师资等多方面进行分析,探讨《全国普通高等学校体育课程教学指导纲要》的具体实施,为营造健康的校园体育教学,倡导"健康第一""终身体育"理念提供有效的理论参考依据.
语种：
中文
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浅谈小学生体育课的重要性与必要性
作者：
刘军;陈杰
期刊：
体育时空 ,2017年(6):93 ISSN：1009-9328
作者机构：
南华大学体育学院 湖南衡阳 421001;[刘军; 陈杰] 南华大学
关键词：
小学体育课;健康体质
摘要：
学生身体素质的提高,学生健康体魄的培养必须从小学开始抓起.小学体育课是学生时代最早接触体育运动的途径之一,让学生从小就养成热爱体育、热爱健身的好习惯,有助于学生健康体质的提高,有助于意志品质的培养,以及为个别体育专项运动的提高打下基础,为学生的终身体育打下基础.
语种：
中文
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Association between fat mass and obesity associated (FTO) gene rs9939609 A/T polymorphism and polycystic ovary syndrome: A systematic review and meta-analysis
作者：
Liu, Ai Ling;Xie, Hui Jun;Xie, Hong Yan;Liu, Jun;Yin, Jie;...
期刊：
BMC Medical Genetics ,2017年18(1):1-7 ISSN：1471-2350
通讯作者：
Peng, Cui Ying
作者机构：
[Yin, Jie; Xie, Hui Jun; Xie, Hong Yan; Hu, Jin Song; Peng, Cui Ying; Liu, Jun; Liu, Ai Ling] Univ South China, Inst Biol Sci, Sch Pharmaceut & Biol Sci, Hengyang 421001, Hunan, Peoples R China.;[Peng, Cui Ying; Liu, Ai Ling] Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.;[Peng, Cui Ying; Liu, Ai Ling] Key Lab Biol Toxicol & Ecol Restorat Hengyang Cit, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Peng, Cui Ying] U;[Peng, Cui Ying] H;Univ South China, Inst Biol Sci, Sch Pharmaceut & Biol Sci, Hengyang 421001, Hunan, Peoples R China.;Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.
关键词：
Polycystic ovary syndrome;FTO;Meta-analysis;Polymorphism
摘要：
Background: Up to now, numerous case-control studies have reported the associations between fat mass and obesity associated (FTO) gene rs9939609 A/T polymorphism and polycystic ovary syndrome (PCOS), however, without a consistent result. Hence we performed current systematic review and meta-analysis to clarify the controversial results. Methods: Case-control studies reporting the relationship of rs9939609 A/T polymorphism and PCOS published before April 2015 were searched in Pubmed database without language restriction. Data was analyzed by Review Manager 5.2. Results: A total of five studies involving 5010 PCOS patients and 5300 controls were included for further metaanalysis. The results of meta-analysis showed that the FTO gene rs9939609 A/T polymorphism was significantly different between PCOS group and control group in different gene models (For AA + AT vs. TT: OR = 1.41, 95% CI = 1.28-1.55, P < 0.00001. For AA vs. AT + TT: OR = 1.54, 95% CI = 1.25-1.89, P < 0.0001. For AA vs. TT: OR = 1. 74, 95% CI = 1.38-2.18, P < 0.00001. For A vs. T: OR = 1.36, 95% CI = 1.25-1.47, P < 0.00001, respectively) suggesting that A allele was a risk factor for PCOS susceptibility. Furthermore, subgroup analysis in Asian and Caucasian ethnicities also found significant association between rs9939609 A/T polymorphism and PCOS (In Asian subgroup: OR = 1.43, 95% CI = 1.29-1.59, P < 0.0001. In Caucasian subgroup: OR = 1.33, 95% CI = 1.08-1.64, P = 0.008) Conclusion: This meta-analysis suggests that rs9939609 A/T polymorphism of FTO gene is associated with PCOS risk, and that A allele is a risk factor for PCOS susceptibility simultaneously. © The Author(s).
语种：
英文
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浅析三分球投篮的教学与训练方法
作者：
刘军;彭春兰
期刊：
当代体育科技 ,2017年7(12):41,43 ISSN：2095-2813
作者机构：
南华大学体育学院 湖南衡阳 421001;[刘军; 彭春兰] 南华大学
关键词：
三分球;投篮;教学;训练方法
摘要：
篮球运动的技战术发展到今天,三分球在比赛中的重要性与日俱增。很多技战术都运用在三分球上,它的总分值也越来越高。在比赛决胜的关键时刻,如能及时成功地组织远投三分球战术配合,将对比赛的胜负趋势起着关键性作用。因此,在日常训练中有目的性地加强三分球的训练是不可或缺的。
语种：
中文
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Tripaddle Posterior Interosseous Artery Flap Design for 3-Finger Defects: An Evaluation of 3 Surgical Approaches
作者：
Li, Kuang-Wen;Song, Da-Jiang;Liu, Jun;Xie, Song-Lin*
期刊：
ANNALS OF PLASTIC SURGERY ,2016年77(4):406-412 ISSN：0148-7043
通讯作者：
Xie, Song-Lin
作者机构：
[Li, Kuang-Wen; Xie, Song-Lin; Liu, Jun] Univ South China, Affiliated Nanhua Hosp, Dept Hand Surg, Hengyang 421002, Hunan, Peoples R China.;[Song, Da-Jiang] Cent S Univ, Xiangya Med Sch, Hunan Prov Canc Hosp, Dept Head & Neck Surg, Changsha, Hunan, Peoples R China.;[Song, Da-Jiang] Cent S Univ, Xiangya Med Sch, Hunan Prov Canc Hosp, Dept Oncol Plast Surg, Changsha, Hunan, Peoples R China.
通讯机构：
[Xie, Song-Lin] U;Univ South China, Affiliated Nanhua Hosp, Dept Hand Surg, Hengyang 421002, Hunan, Peoples R China.
关键词：
Finger;Microsurgery;Perforator flap;Posterior interosseous artery;Tripaddle flap
摘要：
Introduction: The tripaddle posterior interosseous artery (PIA) flap can be used for multifinger defect resurfacing, but interpatient variations in perforator distribution remain an ongoing challenge when using this approach. This study aims to evaluate the efficacy of 3 different tripaddle PIA perforator flap designs according to the PIA perforator distribution for the repair of 3-finger defects. Methods: In accordance with the size of the 3-finger defects and the position of the perforators, a tripaddle flap was designed on the multiple perforators of the descending branch of the PIA in the distal two thirds of the forearm. Patients received 1 of 3 distinct tripaddle PIA perforator flap designs based on perforator distributions of the PIA. Results: Three cases of 3-finger defects were repaired with type A trefoil-shaped tripaddle flaps, whereas 4 cases were repaired with type B modified trefoilshaped tripaddle flaps, and the other 3 cases were repaired with type C chainshaped tripaddle flaps. All flaps survived except 2 paddleswith tip necrosis. After 9.1 months of mean follow-up, 9 of the 10 cases demonstrated satisfactory cosmetic appearance, whereas the last case required a debulking procedure in the second stage. Conclusions: The free tripaddle PIA perforator flap is an effective option for repairing 3-finger skin defects. Various flap designs based on the PIA perforator distribution allow for more individualized treatment approaches. Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.
语种：
英文
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埃兹蛋白(ezrin)基因沉默抑制人前列腺癌PC-3细胞增殖和侵袭
作者：
阳宁;王玲;陈仙;刘俊;罗志刚
期刊：
细胞与分子免疫学杂志 ,2016年32(6):821-824 ISSN：1007-8738
作者机构：
[陈仙; 刘俊; 阳宁] Department of Urology, Second Affiliated Hospital, University of South China, Hengyang 421001, China;[王玲] Hunan University of Environment and Biology, Hengyang 421001, China;[罗志刚] Department of Urology, Second Affiliated Hospital, University of South China, Hengyang 421001, China. *Corresponding author, E-mail: luo6398@sina.com
关键词：
前列腺癌;上皮钙黏素;神经钙黏素
摘要：
目的观察埃兹蛋白（ezrin）在前列腺癌组织中的表达,探讨ezrin基因沉默对人前列腺癌PC-3细胞增殖、侵袭的影响及相关机制。方法收集前列腺癌和良性前列腺增生患者的标本各20例,采用荧光实时定量PCR检测ezrin mRNA水平、Western blot法检测ezrin蛋白水平。培养PC-3细胞,分别用培养液、Scramble siRNA、ezrin siRNA处理48 h;MTT法检测细胞增殖情况,TranswellTM侵袭实验检测细胞侵袭能力,荧光实时定量PCR、Western blot法检测上皮钙黏素、神经钙黏素的mRNA和蛋白水平。结果与良性前列腺增生患者的组织相比,前列腺癌组织中ezrin mRNA、蛋白表达水平均明显增加。与Scramble siRNA处理的PC-3细胞相比,ezrin表达下调后,PC-3细胞增殖受到明显抑制,平均穿膜细胞数、神经钙黏素水平降低,上皮钙黏素水平增加。Scramble siRNA处理的PC-3细胞与单纯培养液处理的PC-3细胞上述指标无明显差异。结论 Ezrin在前列腺癌组织中高表达,ezrin基因沉默能抑制PC-3细胞的增殖和侵袭,同时上调上皮钙黏素表达及下调神经钙黏素表达。
语种：
中文
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基于转塔式快递分拣系统的快递分拣方法
发明/设计人：
胡良斌;李必文;高丽娟;彭聪;刘军
申请/专利权人：
南华大学
申请/专利号：
CN201610408546.X
申请时间：
2016-6-13
公开号：
CN105944969A
公开时间：
2016-9-21
主申请人地址：
421001 湖南省衡阳市蒸湘区常胜西路28号南华大学
摘要：
基于转塔式快递分拣系统的快递分拣方法，包括如下步骤：S01，操作人员扫描包裹的条形码，主控计算机获取目的地信息，然后规划出分拣路线；S02，包裹随着输送带移动，主控计算机接收到包裹感应器的信号后，控制电动缸将包裹推入对应的进料工位上；S03，主控计算机同时进行两项操作：1，控制电动缸使推送手缩回；2，启动并控制步进电机A，包裹被扫到转送工位上，然后启动并控制步进电机B，包裹通过包裹下落口落入包裹收集箱内，分拣结束。本发明优点是：主控计算机自动识别包裹目的地，然后规划快递分拣路径，再将包裹输送至与其地址相对应的包裹收集箱中。相比现有的自动分拣系统，其占地面积小，智能化程度高，分拣效率高。
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SOX10基因突变致Ⅱ型Waardenburg综合征发病的实验研究
作者：
张华;陈红胜;冯永;钱敏飞;李吉平;...
期刊：
中华医学遗传学杂志 ,2016年33(4):466-470 ISSN：1003-9406
作者机构：
新疆医科大学第一附属医院耳鼻咽喉科, 乌鲁木齐, 830011;中南大学湘雅医院耳鼻咽喉科, 长沙, 410008;上海交通大学医学院附属仁济医院耳鼻咽喉科, 200127;[张华] 新疆医科大学第一附属医院耳鼻咽喉科, 乌鲁木齐, 830011;[陈红胜; 冯永] 中南大学湘雅医院耳鼻咽喉科, 长沙, 410008
关键词：
Waardenburg综合征;SOX10基因;MITF基因;基因突变;体外实验
摘要：
目的通过体外实验探讨SOX10基因突变E248fs致Ⅱ型Waardenburg综合征(Waardenburg syndrome,WS)发病的分子机制。 方法野生SOX10及其致病突变E248fs表达质粒瞬时转染293T细胞,用荧光素酶活性检测系统观察野生/突变SOX10蛋白对其靶基因MITF转录活性的调控作用以及突变蛋白对野生SOX10蛋白功能的影响;用生物素标记的含序列cattgtc的DNA寡核苷酸链探针分别沉淀SOX10和E248fs蛋白,检测野生/突变SOX10蛋白与靶基因MITF启动子的结合力;免疫共沉淀检测观察并分析SOX10和E248fs蛋白稳定性变化。 结果E248fs突变蛋白完全失去调控MITF启动子转录活性作用(P<0.01),并对野生SOX10蛋白功能产生显性负效应作用(P<0.05),其与野生SOX10蛋白均可与MITF启动子特异DNA序列cattgtc结合,但较野生SOX10蛋白衰减加快。结论尽管E248fs具有显性负效应,但其稳定性降低,使得其影响靶基因MITF转录活性,导致黑色素合成减少,最终以单倍体剂量不足效应致Ⅱ型WS。
语种：
中文
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Resveratrol induces apoptosis through modulation of the Akt/FoxO3a/Bim pathway in HepG2 cells
作者：
Liu, Mi-Hua* ;Lin, Xiao-Long;Li, Jian;He, Jun;Tan, Tian-Ping;...
期刊：
MOLECULAR MEDICINE REPORTS ,2016年13(2):1689-1694 ISSN：1791-2997
通讯作者：
Liu, Mi-Hua;Zhang, Yuan
作者机构：
[Wu, Shao-Jian; Liu, Mi-Hua; He, Jun; Chen, Yu-Dan; Fu, Hong-Yun; Chen, Li; Yu, Shan; Tan, Tian-Ping; Tian, Wei; Liu, Jun] Univ South China, Affiliated Nanhua Hosp, Dept Clin Lab, 336 Dongfeng South Rd, Hengyang 421001, Hunan, Peoples R China.;[Lin, Xiao-Long] Guangzhou Med Univ, Peoples Hosp Huizhou 3, Dept Pathol, Huizhou 516002, Guangdong, Peoples R China.;[Li, Jian] BoAi Hosp Zhongshan, Dept Ultrason Diag, Zhongshan 528403, Guangdong, Peoples R China.;[Yuan, Cong] First Hosp Changsha, Dept Cardiol, Changsha 410005, Hunan, Peoples R China.;[Zhang, Yuan] Mawangdui Hosp, Dept Pathol, 89 Guhan Rd, Changsha 410016, Hunan, Peoples R China.
通讯机构：
[Liu, Mi-Hua] U;[Zhang, Yuan] M;Univ South China, Affiliated Nanhua Hosp, Dept Clin Lab, 336 Dongfeng South Rd, Hengyang 421001, Hunan, Peoples R China.;Mawangdui Hosp, Dept Pathol, 89 Guhan Rd, Changsha 410016, Hunan, Peoples R China.
关键词：
Apoptosis;Forkhead box O3a;High glucose;Oxidative stress;Resveratrol
摘要：
Resveratrol is a polyphenolic compound found in wine, which is mainly produced by the grapevine and exerts chemopreventive effects against hepatocellular carcinoma. However, the underlying molecular mechanisms have remained to be fully elucidated. The present study assessed whether resveratrol-induced apoptosis was mediated via the activation of the forkhead box O3a (FoxO3a) transcription factor. It was demonstrated that resveratrol treatment induced apoptosis in HepG2 cells, and that this pro-apoptotic effect was accompanied with increases in the expression of apoptotic protein Bim. Following resveratrol treatment, Akt-mediated phosphorylation of FoxO3a was observed to be diminished in HepG2 cells. Furthermore, resveratrol enhanced the nuclear levels of FoxO3a and mediated neuronal death via Bim. The present study demonstrated that resveratrol induced apoptosis in HepG2 cells through activation of the transcription factor FoxO3a and increasing the expression of Bim protein.
语种：
英文
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Hydrogen sulfide attenuates doxorubicin-induced cardiotoxicity. by inhibiting the expression of peroxiredoxin III in H9c2 cells
作者：
Liu, Mi-Hua* ;Lin, Xiao-Long;Yuan, Cong;He, Jun;Tan, Tian-Ping;...
期刊：
MOLECULAR MEDICINE REPORTS ,2016年13(1):367-372 ISSN：1791-2997
通讯作者：
Liu, Mi-Hua
作者机构：
[Wu, Shao-Jian; Liu, Mi-Hua; He, Jun; Chen, Yu-Dan; Fu, Hong-Yun; Chen, Li; Yu, Shan; Tan, Tian-Ping; Tian, Wei; Liu, Jun] Univ South China, Affiliated Nanhua Hosp, Dept Clin Lab, Hengyang 421001, Hunan, Peoples R China.;[Lin, Xiao-Long] Guangzhou Med Univ, Peoples Hosp Huizhou 3, Affiliated Huizhou Hosp, Dept Pathol, Huizhou 516002, Guangdong, Peoples R China.;[Yuan, Cong] First Hosp Changsha, Dept Cardiol, Changsha 410005, Hunan, Peoples R China.;[Li, Jian] Boai Hosp Zhongshan, Dept Ultrason Diag, Zhongshan 528403, Guangdong, Peoples R China.;[Zhang, Yuan] Mawangdui Hosp, Dept Pathol, Changsha 410016, Hunan, Peoples R China.
通讯机构：
[Liu, Mi-Hua] U;Univ South China, Affiliated Nanhua Hosp, Dept Clin Lab, 336 Dongfeng South Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
hydrogen sulfide;peroxiredoxin III;apoptosis;cardiomyocytes
摘要：
Doxorubicin (DOX) is a widely used chemotherapeutic agent, which can give rise to severe cardiotoxicity, limiting its clinical use. Preliminary evidence suggests that hydrogen sulfide (H2S) may exert protective effects on DOX-induced cardiotoxicity. Therefore, the aim of the present study was to investigate whether peroxiredoxin III is involved in the cardioprotection of H2S against DOX-induced cardiotoxicity. The results demonstrated that DOX not only markedly induced injuries, including cytotoxicity and apoptosis, it also increased the expression levels of peroxiredoxin III. Notably, pretreatment with sodium hydrosulfide significantly attenuated the DOX-induced decrease in cell viability and increase in apoptosis, and also reversed the increased expression levels of peroxiredoxin III in H9c2 cardiomyocytes. In addition, pretreatment of the H9c2 cells with N-acetyl-L-cysteine, a scavenger of reactive oxygen species, prior to exposure to DOX markedly decreased the expression levels of peroxiredoxin III. In conclusion, the results of the present study suggested that exogenous H2S attenuates DOX-induced cardiotoxicity by inhibiting the expression of peroxiredoxin III in H9c2 cells. In the present study, the apoptosis of H9c2 cardiomyocytes was assessed using an methyl thiazolyl tetrazolium assay and Hoechst staining. The levels of Prx III and cystathionine-gamma-lyase were examined by western blotting.
语种：
英文
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